Gene description for CIAO1
Gene name cytosolic iron-sulfur assembly component 1
Gene symbol CIAO1
Other names/aliases CIA1
WDR39
Species Homo sapiens
 Database cross references - CIAO1
ExoCarta ExoCarta_9391
Entrez Gene 9391
HGNC 14280
MIM 604333
UniProt O76071  
 CIAO1 identified in exosomes derived from the following tissue/cell type
Hepatocellular carcinoma cells 26054723    
Squamous carcinoma cells 20124223    
 Gene ontology annotations for CIAO1
Molecular Function
    protein binding GO:0005515 IPI
Biological Process
    iron-sulfur cluster assembly GO:0016226 IGI
    positive regulation of cell proliferation GO:0008284 TAS
    chromosome segregation GO:0007059 IEA
    small molecule metabolic process GO:0044281 TAS
    regulation of transcription from RNA polymerase II promoter GO:0006357 TAS
Subcellular Localization
    CIA complex GO:0097361 IDA
    MMXD complex GO:0071817 IDA
 Experiment description of studies that identified CIAO1 in exosomes
1
Experiment ID 235
ISEV standards
✔
EM
EV Biophysical techniques
✔
TSG101|Alix|HSC70|GAPDH
EV Cytosolic markers
✘ EV Membrane markers
✔
HSP90B1
EV Negative markers
✔
qNano
EV Particle analysis
Identified molecule mRNA
Identification method RNA Sequencing
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocellular carcinoma cells
Sample name HKCI-8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RT-PCR
RNA Sequencing
2
Experiment ID 191
ISEV standards
✘ EV Biophysical techniques
✔
Alix
EV Cytosolic markers
✔
CD81|CD9
EV Membrane markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 20124223    
Organism Homo sapiens
Experiment description Hypoxic tumor cell modulates its microenvironment to enhance angiogenic and metastatic potential by secretion of proteins and exosomes.
Authors Park JE, Tan HS, Datta A, Lai RC, Zhang H, Meng W, Lim SK, Sze SK.
Journal name Mol Cell Proteomics
Publication year 2010
Sample Squamous carcinoma cells
Sample name Squamous carcinoma cell (A431)
Isolation/purification methods Differential centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for CIAO1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 FAM96B 51647
Two-hybrid Homo sapiens
2 MCM5 4174
Affinity Capture-MS Homo sapiens
3 USP11 8237
Affinity Capture-MS Homo sapiens
4 MYO1C 4641
Affinity Capture-MS Homo sapiens
5 DNAAF5 54919
Affinity Capture-MS Homo sapiens
6 GAS8  
Affinity Capture-MS Homo sapiens
7 MLF2 8079
Affinity Capture-MS Homo sapiens
8 FLJ22875  
Two-hybrid Homo sapiens
9 CCDC7  
Two-hybrid Homo sapiens
10 CCAR2 57805
Affinity Capture-MS Homo sapiens
View the network image/svg+xml
 Pathways in which CIAO1 is involved
PathwayEvidenceSource
Cytosolic iron-sulfur cluster assembly IEA Reactome





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