Gene description for NARFL
Gene name nuclear prelamin A recognition factor-like
Gene symbol NARFL
Other names/aliases HPRN
IOP1
LET1L
PRN
Species Homo sapiens
 Database cross references - NARFL
ExoCarta ExoCarta_64428
Vesiclepedia VP_64428
Entrez Gene 64428
HGNC 14179
MIM 611118
UniProt Q9H6Q4  
 NARFL identified in sEVs derived from the following tissue/cell type
Mesenchymal stem cells 36408942    
 Gene ontology annotations for NARFL
Molecular Function
    protein binding GO:0005515 IPI
    metal ion binding GO:0046872 IEA
    4 iron, 4 sulfur cluster binding GO:0051539 IEA
Biological Process
    response to hypoxia GO:0001666 IDA
    hematopoietic progenitor cell differentiation GO:0002244 IEA
    regulation of gene expression GO:0010468 IDA
    iron-sulfur cluster assembly GO:0016226 IMP
    intracellular oxygen homeostasis GO:0032364 IDA
Subcellular Localization
    cytosolic [4Fe-4S] assembly targeting complex GO:0097361 IBA
    cytosolic [4Fe-4S] assembly targeting complex GO:0097361 IDA
 Experiment description of studies that identified NARFL in sEVs
1
Experiment ID 488
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD81|CD63|GAPDH|SDCBP|LAMP1|TFRC|UCHL1|FLOT2|LAMP2|FLOT1|ICAM1|RAB5B|CD151|RAB35|TSG101|RAB5A|CD82
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Homo sapiens
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Mesenchymal stem cells
Sample name UCMSC
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
 Protein-protein interactions for NARFL
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 CCNO  
Affinity Capture-MS Homo sapiens
2 DDX6 1656
Co-fractionation Homo sapiens
3 TP53 7157
Affinity Capture-MS Homo sapiens
4 CIAO1 9391
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
5 NUBP2 10101
Affinity Capture-MS Homo sapiens
6 INPPL1 3636
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
7 FAM96A  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
8 ARNT 405
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
9 EGFR 1956
Negative Genetic Homo sapiens
10 DCAF16 54876
Affinity Capture-MS Homo sapiens
11 APP 351
Reconstituted Complex Homo sapiens
12 FAM96B 51647
Affinity Capture-MS Homo sapiens
13 APEX1 328
Affinity Capture-RNA Homo sapiens
14 GSTM3 2947
Co-fractionation Homo sapiens
15 MMS19 64210
Affinity Capture-MS Homo sapiens
16 DTX3  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
17 MYH9 4627
Affinity Capture-MS Homo sapiens
18 OGT 8473
Co-fractionation Homo sapiens
19 NAA11 84779
Affinity Capture-MS Homo sapiens
20 MOV10 4343
Affinity Capture-RNA Homo sapiens
21 RGS20 8601
Affinity Capture-MS Homo sapiens
22 KRAS 3845
Negative Genetic Homo sapiens
23 STAT1 6772
Co-fractionation Homo sapiens
24 ZBTB39  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
25 NAA10 8260
Affinity Capture-MS Homo sapiens
26 WDFY1 57590
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
View the network image/svg+xml
 Pathways in which NARFL is involved
PathwayEvidenceSource
Cytosolic iron-sulfur cluster assembly IEA Reactome
Metabolism IEA Reactome





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