Gene description for FAM96B
Gene name family with sequence similarity 96, member B
Gene symbol FAM96B
Other names/aliases CGI-128
MIP18
Species Homo sapiens
 Database cross references - FAM96B
ExoCarta ExoCarta_51647
Vesiclepedia VP_51647
Entrez Gene 51647
HGNC 24261
MIM 614778
UniProt Q9Y3D0  
 FAM96B identified in exosomes derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Hepatocellular carcinoma cells 26054723    
 Gene ontology annotations for FAM96B
Molecular Function
    protein binding GO:0005515 IPI
Biological Process
    chromosome segregation GO:0007059 IMP
    iron-sulfur cluster assembly GO:0016226 IMP
    protein maturation by iron-sulfur cluster transfer GO:0097428 IBA
    protein maturation by iron-sulfur cluster transfer GO:0097428 IMP
    protein maturation by [4Fe-4S] cluster transfer GO:0106035 IEA
Subcellular Localization
    nucleus GO:0005634 IDA
    nucleoplasm GO:0005654 IDA
    cytoplasm GO:0005737 IDA
    spindle GO:0005819 IDA
    cytosol GO:0005829 IDA
    midbody GO:0030496 IEA
    MMXD complex GO:0071817 IDA
    cytosolic [4Fe-4S] assembly targeting complex GO:0097361 IBA
    cytosolic [4Fe-4S] assembly targeting complex GO:0097361 IDA
 Experiment description of studies that identified FAM96B in exosomes
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 235
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|Alix|HSC70|GAPDH
Enriched markers
✔
HSP90B1
Negative markers
✔
qNano
Particle analysis
Identified molecule mRNA
Identification method RNA Sequencing
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors "He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N"
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocellular carcinoma cells
Sample name HKCI-8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RT-PCR
RNA Sequencing
 Protein-protein interactions for FAM96B
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 UBE2H 7328
Affinity Capture-MS Homo sapiens
2 SLFN11 91607
Affinity Capture-MS Homo sapiens
3 PRIM2 5558
Affinity Capture-MS Homo sapiens
4 RIT1 6016
Negative Genetic Homo sapiens
5 UQCRFS1 7386
Affinity Capture-MS Homo sapiens
6 Mms19  
Affinity Capture-MS Mus musculus
7 PAXIP1  
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
8 ELP6 54859
Affinity Capture-MS Homo sapiens
9 USPL1  
Affinity Capture-MS Homo sapiens
10 WNK1 65125
Affinity Capture-MS Homo sapiens
11 ERCC2 2068
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
12 TRIM66  
Affinity Capture-MS Homo sapiens
13 TDRD3  
Affinity Capture-MS Homo sapiens
14 CNP 1267
Affinity Capture-MS Homo sapiens
15 PPAT 5471
Affinity Capture-MS Homo sapiens
16 PCBP3 54039
Affinity Capture-MS Homo sapiens
17 DNAJB6 10049
Affinity Capture-MS Homo sapiens
18 PPP1CC 5501
Affinity Capture-MS Homo sapiens
19 KIAA1429 25962
Affinity Capture-MS Homo sapiens
20 NARFL 64428
Affinity Capture-MS Homo sapiens
21 MCM2 4171
Affinity Capture-MS Homo sapiens
22 PRIM1  
Affinity Capture-MS Homo sapiens
23 FMNL2 114793
Affinity Capture-MS Homo sapiens
24 ABCE1 6059
Affinity Capture-MS Homo sapiens
25 ELP4  
Affinity Capture-MS Homo sapiens
26 MSL2  
Affinity Capture-MS Homo sapiens
27 CEP57  
Affinity Capture-MS Homo sapiens
28 SNX27 81609
Affinity Capture-MS Homo sapiens
29 MAK16  
Affinity Capture-MS Homo sapiens
30 FBXL5  
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
31 BAG5 9529
Affinity Capture-MS Homo sapiens
32 FAM96A  
Affinity Capture-MS Homo sapiens
33 POLD1 5424
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
34 TOP3B 8940
Affinity Capture-MS Homo sapiens
35 CTU2 348180
Affinity Capture-MS Homo sapiens
36 GGT7 2686
Affinity Capture-MS Homo sapiens
37 SPIN2A  
Affinity Capture-MS Homo sapiens
38 ILVBL 10994
Affinity Capture-MS Homo sapiens
39 MPPED1  
Affinity Capture-MS Homo sapiens
40 COMTD1 118881
Affinity Capture-MS Homo sapiens
41 CDKAL1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
42 POLE  
Affinity Capture-MS Homo sapiens
43 CEP76  
Affinity Capture-MS Homo sapiens
44 SON 6651
Affinity Capture-MS Homo sapiens
45 MLF2 8079
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
46 RTEL1 51750
Affinity Capture-MS Homo sapiens
47 YAE1D1  
Affinity Capture-MS Homo sapiens
48 USP4 7375
Two-hybrid Homo sapiens
49 TUBB2A 7280
Affinity Capture-MS Homo sapiens
50 CTU1 90353
Affinity Capture-MS Homo sapiens
51 CIAO1 9391
Two-hybrid Homo sapiens
Co-fractionation Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
Co-fractionation Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
52 FAM60A  
Affinity Capture-MS Homo sapiens
53 CDC42EP1 11135
Affinity Capture-MS Homo sapiens
54 ELP3 55140
Affinity Capture-MS Homo sapiens
55 CUL3 8452
Affinity Capture-MS Homo sapiens
56 MTNR1A  
Two-hybrid Homo sapiens
57 RIPK4  
Affinity Capture-MS Homo sapiens
58 ELP2 55250
Affinity Capture-MS Homo sapiens
59 NTHL1  
Affinity Capture-MS Homo sapiens
60 MSL1 339287
Affinity Capture-MS Homo sapiens
61 DUSP9  
Affinity Capture-MS Homo sapiens
62 RAF1 5894
Affinity Capture-MS Homo sapiens
63 Mad2l1 56150
Affinity Capture-MS Mus musculus
64 CETN2 1069
Affinity Capture-MS Homo sapiens
65 C9orf72  
Affinity Capture-MS Homo sapiens
66 PAFAH1B1 5048
Affinity Capture-MS Homo sapiens
67 PLEKHA4 57664
Affinity Capture-MS Homo sapiens
68 SLC25A6 293
Affinity Capture-MS Homo sapiens
69 IREB2 3658
Affinity Capture-Western Homo sapiens
70 DNA2  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
71 ACAA2 10449
Affinity Capture-MS Homo sapiens
72 TYW1  
Affinity Capture-MS Homo sapiens
73 EIF5A2 56648
Affinity Capture-MS Homo sapiens
74 ZNF536  
Affinity Capture-MS Homo sapiens
75 EDC3 80153
Affinity Capture-MS Homo sapiens
76 NUP35 129401
Affinity Capture-MS Homo sapiens
77 FMNL3 91010
Affinity Capture-MS Homo sapiens
78 CDK1 983
Affinity Capture-MS Homo sapiens
79 MMS19 64210
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
80 ERRFI1 54206
Two-hybrid Homo sapiens
81 KIAA1644  
Affinity Capture-MS Homo sapiens
82 GLRX3 10539
Affinity Capture-MS Homo sapiens
83 ELP5 23587
Affinity Capture-MS Homo sapiens
84 KRAS 3845
Negative Genetic Homo sapiens
85 EIF3M 10480
Affinity Capture-MS Homo sapiens
86 DCP2  
Affinity Capture-MS Homo sapiens
87 DDX56  
Affinity Capture-MS Homo sapiens
88 EIF5AL1 143244
Affinity Capture-MS Homo sapiens
View the network image/svg+xml
 Pathways in which FAM96B is involved
PathwayEvidenceSource
Cytosolic iron-sulfur cluster assembly IEA Reactome
Metabolism IEA Reactome





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