Gene description for WDFY1
Gene name WD repeat and FYVE domain containing 1
Gene symbol WDFY1
Other names/aliases FENS-1
WDF1
ZFYVE17
Species Homo sapiens
 Database cross references - WDFY1
ExoCarta ExoCarta_57590
Entrez Gene 57590
HGNC 20451
UniProt Q8IWB7  
 WDFY1 identified in exosomes derived from the following tissue/cell type
Endothelial cells 26027894    
Thymus 23844026    
 Gene ontology annotations for WDFY1
Molecular Function
    zinc ion binding GO:0008270 NAS
    1-phosphatidylinositol binding GO:0005545 IDA
Biological Process
    biological_process GO:0008150 ND
Subcellular Localization
    nucleus GO:0005634 IDA
    early endosome GO:0005769 IDA
    cytosol GO:0005829 IDA
 Experiment description of studies that identified WDFY1 in exosomes
1
Experiment ID 226
ISEV standards
EM
EV Biophysical techniques
GAPDH
EV Cytosolic markers
CD9|FLOT1
EV Membrane markers
LMNA|H2AFX|ATP5A1|TOMM20
EV Negative markers
EV Particle analysis
Identified molecule mRNA
Identification method Small RNA sequencing (Illumina HiSeq 2000 (Solexa)
PubMed ID 26027894    
Organism Homo sapiens
Experiment description Quantitative and qualitative analysis of small RNAs in human endothelial cells and exosomes provides insights into localized RNA processing, degradation and sorting
Authors Bas W. M. van Balkom, Almut S. Eisele, D. Michiel Pegtel, Sander Bervoets, Marianne C. Verhaar
Journal name Journal of Extracellular Vesicles
Publication year 2015
Sample Endothelial cells
Sample name HMEC-1
Isolation/purification methods Differential ultracentrifugation
Sucrose density gradient
Flotation density 1.10 g/mL
Molecules identified in the study miRNA
Protein
snoRNA
lncRNA
yRNA
snRNA
mRNA
ncRNA
mtRNA
vtRNA
scaRNA
lincRNA
Methods used in the study Small RNA sequencing (Illumina HiSeq 2000 (Solexa)
Western blotting
2
Experiment ID 217
ISEV standards
EM
EV Biophysical techniques
TSG101
EV Cytosolic markers
CD81|CD9|CD63
EV Membrane markers
EV Negative markers
NTA
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23844026    
Organism Homo sapiens
Experiment description Characterization of human thymic exosomes.
Authors Skogberg G, Gudmundsdottir J, van der Post S, Sandstrom K, Bruhn S, Benson M, Mincheva-Nilsson L, Baranov V, Telemo E, Ekwall O.
Journal name PLoS One
Publication year 2013
Sample Thymus
Sample name Normal-Thymus
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for WDFY1
  Protein Interactor ExoCarta ID Identification method PubMed Species
No interactions are found.
 Pathways in which WDFY1 is involved
No pathways found





Perform bioinformatics analysis of your extracellular vesicle data set using FunRich, a open access standalone tool. NEW UPDATED VERSION OF FunRich available for download (12/09/2016) from here