Gene description for SIRT5
Gene name sirtuin 5
Gene symbol SIRT5
Other names/aliases SIR2L5
Species Homo sapiens
 Database cross references - SIRT5
ExoCarta ExoCarta_23408
Vesiclepedia VP_23408
Entrez Gene 23408
HGNC 14933
MIM 604483
UniProt Q9NXA8  
 SIRT5 identified in exosomes derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Hepatocellular carcinoma cells 26054723    
Mesenchymal stem cells 36408942    
 Gene ontology annotations for SIRT5
Molecular Function
    NAD+-protein poly-ADP-ribosyltransferase activity GO:0003950 TAS
    zinc ion binding GO:0008270 IDA
    histone deacetylase activity, NAD-dependent GO:0017136 IBA
    NAD-dependent protein lysine deacetylase activity GO:0034979 TAS
    protein-malonyllysine demalonylase activity GO:0036054 IBA
    protein-malonyllysine demalonylase activity GO:0036054 IDA
    protein-succinyllysine desuccinylase activity GO:0036055 IBA
    protein-succinyllysine desuccinylase activity GO:0036055 IDA
    protein-glutaryllysine deglutarylase activity GO:0061697 IBA
    protein-glutaryllysine deglutarylase activity GO:0061697 IDA
    NAD+ binding GO:0070403 IBA
    NAD+ binding GO:0070403 IDA
    NAD+-protein ADP-ribosyltransferase activity GO:1990404 TAS
Biological Process
    protein deacetylation GO:0006476 IDA
    protein deacetylation GO:0006476 IDA
    mitochondrion organization GO:0007005 TAS
    regulation of ketone biosynthetic process GO:0010566 ISS
    negative regulation of cardiac muscle cell apoptotic process GO:0010667 IEA
    response to nutrient levels GO:0031667 IEA
    protein demalonylation GO:0036046 IDA
    peptidyl-lysine demalonylation GO:0036047 IDA
    protein desuccinylation GO:0036048 IDA
    peptidyl-lysine desuccinylation GO:0036049 IDA
    protein deglutarylation GO:0061698 IDA
    negative regulation of reactive oxygen species metabolic process GO:2000378 IDA
Subcellular Localization
    nucleus GO:0005634 IBA
    nucleus GO:0005634 TAS
    mitochondrion GO:0005739 HTP
    mitochondrion GO:0005739 IBA
    mitochondrion GO:0005739 IDA
    mitochondrial intermembrane space GO:0005758 IDA
    mitochondrial matrix GO:0005759 IBA
    mitochondrial matrix GO:0005759 IDA
    mitochondrial matrix GO:0005759 TAS
    cytosol GO:0005829 IBA
    cytosol GO:0005829 IDA
 Experiment description of studies that identified SIRT5 in exosomes
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 234
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|Alix|HSC70|GAPDH
Enriched markers
✔
HSP90B1
Negative markers
✔
qNano
Particle analysis
Identified molecule mRNA
Identification method RNA Sequencing
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors "He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N"
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocellular carcinoma cells
Sample name HKCI-C3
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RT-PCR
RNA Sequencing
6
Experiment ID 488
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD81|CD63|GAPDH|SDCBP|LAMP1|TFRC|UCHL1|FLOT2|LAMP2|FLOT1|ICAM1|RAB5B|CD151|RAB35|TSG101|RAB5A|CD82
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Homo sapiens
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Mesenchymal stem cells
Sample name UCMSC
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
 Protein-protein interactions for SIRT5
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 MPST 4357
Co-fractionation Homo sapiens
2 RELA 5970
Affinity Capture-Western Homo sapiens
3 ZSCAN18  
Affinity Capture-MS Homo sapiens
4 GLS 2744
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
5 GBP6 163351
Affinity Capture-MS Homo sapiens
6 RBM5  
Affinity Capture-MS Homo sapiens
7 VDR  
Affinity Capture-MS Homo sapiens
8 CKAP2L  
Affinity Capture-MS Homo sapiens
9 HOXB5  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
10 TAL1  
Affinity Capture-MS Homo sapiens
11 PARP12 64761
Affinity Capture-MS Homo sapiens
12 SNCG 6623
Affinity Capture-MS Homo sapiens
13 GYG2  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
14 THEM4  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
15 HNRNPD 3184
Affinity Capture-MS Homo sapiens
16 SEMA4A  
Affinity Capture-MS Homo sapiens
17 SIRT3  
Affinity Capture-Western Homo sapiens
18 GHRL  
Affinity Capture-MS Homo sapiens
19 Pik3r2  
Affinity Capture-MS Mus musculus
20 CWC15  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
21 APP 351
Reconstituted Complex Homo sapiens
22 BAG6 7917
Affinity Capture-MS Homo sapiens
23 ZMAT2  
Affinity Capture-MS Homo sapiens
24 AURKB 9212
Affinity Capture-MS Homo sapiens
25 RABL3 285282
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
26 CAPZB 832
Affinity Capture-MS Homo sapiens
27 IDH2 3418
Affinity Capture-Western Homo sapiens
28 HOXA4  
Affinity Capture-MS Homo sapiens
29 SGTB  
Affinity Capture-MS Homo sapiens
30 PCDH10 57575
Affinity Capture-MS Homo sapiens
31 HECW2  
Affinity Capture-MS Homo sapiens
32 SNX20  
Affinity Capture-MS Homo sapiens
33 HOXA5  
Affinity Capture-MS Homo sapiens
34 SORT1 6272
Affinity Capture-MS Homo sapiens
35 PCCB 5096
Affinity Capture-MS Homo sapiens
36 HOXC5  
Affinity Capture-MS Homo sapiens
37 ZNF776  
Affinity Capture-MS Homo sapiens
38 HDLBP 3069
Affinity Capture-MS Homo sapiens
39 CPLX3  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
40 TMEM184A  
Affinity Capture-MS Homo sapiens
41 DMD 1756
Affinity Capture-MS Homo sapiens
42 YBEY  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
43 MALSU1  
Affinity Capture-MS Homo sapiens
44 CDR2L  
Affinity Capture-MS Homo sapiens
45 METTL20  
Affinity Capture-MS Homo sapiens
46 DOCK3  
Affinity Capture-MS Homo sapiens
47 WDFY1 57590
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
48 HBZ  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
49 BRD4 23476
Affinity Capture-MS Homo sapiens
50 SP100 6672
Affinity Capture-MS Homo sapiens
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