Gene description for SIRT5
Gene name sirtuin 5
Gene symbol SIRT5
Other names/aliases SIR2L5
Species Homo sapiens
 Database cross references - SIRT5
ExoCarta ExoCarta_23408
Entrez Gene 23408
HGNC 14933
MIM 604483
UniProt Q9NXA8  
 SIRT5 identified in exosomes derived from the following tissue/cell type
Hepatocellular carcinoma cells 26054723    
 Gene ontology annotations for SIRT5
Molecular Function
    zinc ion binding GO:0008270 IDA
    protein-succinyllysine desuccinylase activity GO:0036055 IDA
    NAD+ ADP-ribosyltransferase activity GO:0003950 TAS
    protein-malonyllysine demalonylase activity GO:0036054 IDA
    NAD+ binding GO:0070403 IDA
Biological Process
    protein desuccinylation GO:0036048 IDA
    protein deacetylation GO:0006476 IDA
    chromatin silencing GO:0006342 TAS
    protein ADP-ribosylation GO:0006471 TAS
    peptidyl-lysine desuccinylation GO:0036049 IDA
    negative regulation of reactive oxygen species metabolic process GO:2000378 IDA
    peptidyl-lysine demalonylation GO:0036047 IDA
Subcellular Localization
    mitochondrial intermembrane space GO:0005758 IDA
    nucleus GO:0005634 TAS
    mitochondrion GO:0005739 IDA
    cytosol GO:0005829 IDA
    mitochondrial matrix GO:0005759 IDA
 Experiment description of studies that identified SIRT5 in exosomes
1
Experiment ID 234
ISEV standards
EM
EV Biophysical techniques
TSG101|Alix|HSC70|GAPDH
EV Cytosolic markers
EV Membrane markers
HSP90B1
EV Negative markers
qNano
EV Particle analysis
Identified molecule mRNA
Identification method RNA Sequencing
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocellular carcinoma cells
Sample name HKCI-C3
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RT-PCR
RNA Sequencing
 Protein-protein interactions for SIRT5
  Protein Interactor ExoCarta ID Identification method PubMed Species
No interactions are found.
 Pathways in which SIRT5 is involved
No pathways found





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