Gene description for GOLGA3
Gene name golgin A3
Gene symbol GOLGA3
Other names/aliases GCP170
MEA-2
Species Homo sapiens
 Database cross references - GOLGA3
ExoCarta ExoCarta_2802
Vesiclepedia VP_2802
Entrez Gene 2802
HGNC 4426
MIM 602581
UniProt Q08378  
 GOLGA3 identified in exosomes derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Colorectal cancer cells 23161513    
Colorectal cancer cells 23161513    
Colorectal cancer cells 23161513    
Mesenchymal stem cells 36408942    
Retinal pigment epithelial cells 35333565    
Retinal pigment epithelial cells 35333565    
Urine 21595033    
Urine 21595033    
Urine 21595033    
 Gene ontology annotations for GOLGA3
Molecular Function
    protein binding GO:0005515 IPI
    cadherin binding GO:0045296 HDA
Subcellular Localization
    Golgi membrane GO:0000139 IDA
    nucleoplasm GO:0005654 IDA
    nucleolus GO:0005730 IDA
    Golgi apparatus GO:0005794 IDA
    Golgi apparatus GO:0005794 TAS
    cytosol GO:0005829 IDA
    membrane GO:0016020 HDA
    Golgi cisterna membrane GO:0032580 IEA
 Experiment description of studies that identified GOLGA3 in exosomes
1
Experiment ID 489
MISEV standards
EM
Biophysical techniques
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
Canx
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
EM
Biophysical techniques
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
Canx
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
EM
Biophysical techniques
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
Canx
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
EM
Biophysical techniques
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
Canx
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 207
MISEV standards
EM
Biophysical techniques
TSG101|HSP70|FLOT1
Enriched markers
VDAC
Negative markers
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23161513    
Organism Homo sapiens
Experiment description Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS.
Authors "Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ."
Journal name Mol Cell Proteomics
Publication year 2012
Sample Colorectal cancer cells
Sample name DKO-1
Isolation/purification methods Differential centrifugation
Filtration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
6
Experiment ID 208
MISEV standards
EM
Biophysical techniques
TSG101|HSP70|FLOT1
Enriched markers
VDAC
Negative markers
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23161513    
Organism Homo sapiens
Experiment description Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS.
Authors "Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ."
Journal name Mol Cell Proteomics
Publication year 2012
Sample Colorectal cancer cells
Sample name Dks-8
Isolation/purification methods Differential centrifugation
Filtration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
7
Experiment ID 209
MISEV standards
Biophysical techniques
TSG101|HSP70|FLOT1
Enriched markers
VDAC
Negative markers
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23161513    
Organism Homo sapiens
Experiment description Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS.
Authors "Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ."
Journal name Mol Cell Proteomics
Publication year 2012
Sample Colorectal cancer cells
Sample name DLD-1
Isolation/purification methods Differential centrifugation
Filtration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
8
Experiment ID 488
MISEV standards
EM
Biophysical techniques
CD9|CD81|CD63|GAPDH|SDCBP|LAMP1|TFRC|UCHL1|FLOT2|LAMP2|FLOT1|ICAM1|RAB5B|CD151|RAB35|TSG101|RAB5A|CD82
Enriched markers
CANX
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Homo sapiens
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Mesenchymal stem cells
Sample name UCMSC
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
9
Experiment ID 834
MISEV standards
EM
Biophysical techniques
LAMP2|CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
Enriched markers
CANX
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P"
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
10
Experiment ID 835
MISEV standards
EM
Biophysical techniques
CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
Enriched markers
CANX
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P"
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
11
Experiment ID 193
MISEV standards
EM
Biophysical techniques
CD63|CD9
Enriched markers
PHB
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 21595033    
Organism Homo sapiens
Experiment description Proteomic analysis of urinary exosomes from patients of early IgA nephropathy and thin basement membrane nephropathy.
Authors "Moon PG, Lee JE, You S, Kim TK, Cho JH, Kim IS, Kwon TH, Kim CD, Park SH, Hwang D, Kim YL, Baek MC."
Journal name Proteomics
Publication year 2011
Sample Urine
Sample name Urine - Normal
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
12
Experiment ID 194
MISEV standards
Biophysical techniques
Enriched markers
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 21595033    
Organism Homo sapiens
Experiment description Proteomic analysis of urinary exosomes from patients of early IgA nephropathy and thin basement membrane nephropathy.
Authors "Moon PG, Lee JE, You S, Kim TK, Cho JH, Kim IS, Kwon TH, Kim CD, Park SH, Hwang D, Kim YL, Baek MC."
Journal name Proteomics
Publication year 2011
Sample Urine
Sample name Urine - Patients of basement membrane nephropathy
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
13
Experiment ID 195
MISEV standards
Biophysical techniques
Enriched markers
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 21595033    
Organism Homo sapiens
Experiment description Proteomic analysis of urinary exosomes from patients of early IgA nephropathy and thin basement membrane nephropathy.
Authors "Moon PG, Lee JE, You S, Kim TK, Cho JH, Kim IS, Kwon TH, Kim CD, Park SH, Hwang D, Kim YL, Baek MC."
Journal name Proteomics
Publication year 2011
Sample Urine
Sample name Urine - Patients of early IgA nephropathy
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for GOLGA3
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 UBE2H 7328
Affinity Capture-MS Homo sapiens
2 GJD3  
Proximity Label-MS Homo sapiens
3 MTCH1 23787
Proximity Label-MS Homo sapiens
4 LGR4 55366
Affinity Capture-MS Homo sapiens
5 OCLN 100506658
Proximity Label-MS Homo sapiens
6 MARCKS 4082
Proximity Label-MS Homo sapiens
7 LAMP3  
Proximity Label-MS Homo sapiens
8 SYCE1  
Affinity Capture-MS Homo sapiens
9 GJA1 2697
Proximity Label-MS Homo sapiens
10 YWHAE 7531
Affinity Capture-MS Homo sapiens
11 FUOM  
Affinity Capture-MS Homo sapiens
12 GNS 2799
Co-fractionation Homo sapiens
13 UBE3A 7337
Affinity Capture-MS Homo sapiens
14 HSD17B11 51170
Proximity Label-MS Homo sapiens
15 LAMP2 3920
Proximity Label-MS Homo sapiens
16 DNAJC25 548645
Proximity Label-MS Homo sapiens
17 TROVE2 6738
Co-fractionation Homo sapiens
18 PTPN1 5770
Proximity Label-MS Homo sapiens
19 STX4 6810
Proximity Label-MS Homo sapiens
20 TGM2 7052
Co-fractionation Homo sapiens
21 GOLGA7 51125
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
22 TMA16  
Affinity Capture-MS Homo sapiens
23 CENPU  
Affinity Capture-MS Homo sapiens
24 PPP1R12A 4659
Co-fractionation Homo sapiens
25 B3GAT1  
Proximity Label-MS Homo sapiens
26 WHAMMP3  
Affinity Capture-MS Homo sapiens
27 TSNAX 7257
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
28 SPAG9 9043
Co-fractionation Homo sapiens
29 RAB3B 5865
Proximity Label-MS Homo sapiens
30 SPATA6L  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
31 CCDC53 51019
Affinity Capture-MS Homo sapiens
32 FBXW8 26259
Affinity Capture-MS Homo sapiens
33 SSR1 6745
Proximity Label-MS Homo sapiens
34 OBSL1 23363
Affinity Capture-MS Homo sapiens
35 SMC2 10592
Co-fractionation Homo sapiens
36 HMGA1 3159
Cross-Linking-MS (XL-MS) Homo sapiens
37 EXD2  
Proximity Label-MS Homo sapiens
38 GOPC 57120
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
39 SLC25A46 91137
Proximity Label-MS Homo sapiens
40 RAB35 11021
Proximity Label-MS Homo sapiens
41 LRRC36  
Affinity Capture-MS Homo sapiens
42 EBAG9 9166
Proximity Label-MS Homo sapiens
43 MARCH5  
Proximity Label-MS Homo sapiens
44 METTL7A 25840
Proximity Label-MS Homo sapiens
45 LAMTOR1 55004
Proximity Label-MS Homo sapiens
46 NUP155 9631
Proximity Label-MS Homo sapiens
47 RAB4A 5867
Proximity Label-MS Homo sapiens
48 PFN1 5216
Proximity Label-MS Homo sapiens
49 KRT8 3856
Proximity Label-MS Homo sapiens
50 S100A2 6273
Affinity Capture-MS Homo sapiens
51 MTCH2 23788
Proximity Label-MS Homo sapiens
52 EMD 2010
Proximity Label-MS Homo sapiens
53 TAF10 6881
Affinity Capture-MS Homo sapiens
54 WHSC1 7468
Affinity Capture-MS Homo sapiens
55 CXADR 1525
Proximity Label-MS Homo sapiens
56 STAU1 6780
Affinity Capture-MS Homo sapiens
57 PSMD14 10213
Affinity Capture-MS Homo sapiens
58 GOLGA2 2801
Proximity Label-MS Homo sapiens
59 DIABLO 56616
Proximity Label-MS Homo sapiens
60 DNAJC5 80331
Proximity Label-MS Homo sapiens
61 CDH1 999
Proximity Label-MS Homo sapiens
62 DIRAS3  
Proximity Label-MS Homo sapiens
63 NTRK1 4914
Affinity Capture-MS Homo sapiens
64 SYNC  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
65 FAM134A 79137
Affinity Capture-MS Homo sapiens
66 SYNCRIP 10492
Affinity Capture-MS Homo sapiens
67 LAMP1 3916
Proximity Label-MS Homo sapiens
68 DNAJA1 3301
Co-fractionation Homo sapiens
69 STX6 10228
Proximity Label-MS Homo sapiens
70 MYH10 4628
Co-fractionation Homo sapiens
71 RHOT2 89941
Proximity Label-MS Homo sapiens
72 ELOVL5 60481
Proximity Label-MS Homo sapiens
73 SEC61B 10952
Proximity Label-MS Homo sapiens
74 MYH9 4627
Co-fractionation Homo sapiens
75 KIF3B 9371
Co-fractionation Homo sapiens
76 REEP5 7905
Proximity Label-MS Homo sapiens
77 ANLN 54443
Affinity Capture-MS Homo sapiens
78 PER2  
Affinity Capture-MS Homo sapiens
79 GOLGA3 2802
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
80 RPN2 6185
Proximity Label-MS Homo sapiens
81 RAB7A 7879
Proximity Label-MS Homo sapiens
82 AHCYL1 10768
Co-fractionation Homo sapiens
83 CIAO1 9391
Affinity Capture-MS Homo sapiens
84 LRRC59 55379
Proximity Label-MS Homo sapiens
85 SMARCA5 8467
Co-fractionation Homo sapiens
86 ZSCAN1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
87 Cul3  
Affinity Capture-MS Mus musculus
88 MEX3A  
Affinity Capture-RNA Homo sapiens
89 U2AF2 11338
Co-fractionation Homo sapiens
90 HSD3B7 80270
Proximity Label-MS Homo sapiens
91 FKBP8 23770
Proximity Label-MS Homo sapiens
92 OCIAD1 54940
Proximity Label-MS Homo sapiens
93 CEP250 11190
Affinity Capture-MS Homo sapiens
94 FGFR2 2263
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
95 TPM1 7168
Affinity Capture-MS Homo sapiens
96 Uso1 56041
Affinity Capture-MS Mus musculus
97 CNTROB  
Affinity Capture-MS Homo sapiens
98 RAB9A 9367
Proximity Label-MS Homo sapiens
99 GOLGA1  
Proximity Label-MS Homo sapiens
100 NDC80 10403
Proximity Label-MS Homo sapiens
101 JUNB  
Co-fractionation Homo sapiens
102 CUL7 9820
Affinity Capture-MS Homo sapiens
103 MFN2 9927
Proximity Label-MS Homo sapiens
104 KRT37 8688
Affinity Capture-MS Homo sapiens
105 HSPA9 3313
Co-fractionation Homo sapiens
106 Prkar2a 19087
Affinity Capture-MS Mus musculus
107 AKAP1 8165
Proximity Label-MS Homo sapiens
108 Prkacb 18749
Affinity Capture-MS Mus musculus
109 MTA2 9219
Co-fractionation Homo sapiens
110 C17orf80 55028
Proximity Label-MS Homo sapiens
111 KRT27 342574
Affinity Capture-MS Homo sapiens
112 TRAF2 7186
Affinity Capture-MS Homo sapiens
113 BFSP1  
Affinity Capture-MS Homo sapiens
114 Itsn2  
Affinity Capture-MS Mus musculus
115 USP36  
Affinity Capture-MS Homo sapiens
116 ATXN7  
Two-hybrid Homo sapiens
117 PDHA1 5160
Affinity Capture-MS Homo sapiens
118 LCK 3932
Proximity Label-MS Homo sapiens
119 SMURF1 57154
Affinity Capture-MS Homo sapiens
120 ELAVL1 1994
Affinity Capture-RNA Homo sapiens
121 C15orf59  
Affinity Capture-MS Homo sapiens
122 GORASP1 64689
Proximity Label-MS Homo sapiens
123 ANAPC2 29882
Proximity Label-MS Homo sapiens
124 UFL1 23376
Affinity Capture-MS Homo sapiens
125 PXMP2  
Proximity Label-MS Homo sapiens
126 RAB5A 5868
Proximity Label-MS Homo sapiens
127 ERGIC2 51290
Proximity Label-MS Homo sapiens
128 RAB2A 5862
Proximity Label-MS Homo sapiens
129 SEC62 7095
Proximity Label-MS Homo sapiens
130 HNRNPC 3183
Co-fractionation Homo sapiens
131 SORT1 6272
Affinity Capture-MS Homo sapiens
132 RAB11A 8766
Proximity Label-MS Homo sapiens
133 KXD1 79036
Affinity Capture-MS Homo sapiens
134 EPHA2 1969
Proximity Label-MS Homo sapiens
135 EZR 7430
Proximity Label-MS Homo sapiens
136 DNAJC1 64215
Proximity Label-MS Homo sapiens
137 RAB5C 5878
Proximity Label-MS Homo sapiens
138 CCDC67  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
139 PRMT1 3276
Affinity Capture-MS Homo sapiens
140 CAV1 857
Proximity Label-MS Homo sapiens
141 SRR 63826
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
142 WDHD1  
Co-fractionation Homo sapiens
143 DERL1 79139
Proximity Label-MS Homo sapiens
144 TRAF1 7185
Affinity Capture-MS Homo sapiens
View the network image/svg+xml



Perform bioinformatics analysis of your extracellular vesicle data set using FunRich, a open access standalone tool. NEW UPDATED VERSION OF FunRich available for download (12/09/2016) from here