Gene description for GNS
Gene name glucosamine (N-acetyl)-6-sulfatase
Gene symbol GNS
Other names/aliases G6S
Species Homo sapiens
 Database cross references - GNS
ExoCarta ExoCarta_2799
Entrez Gene 2799
HGNC 4422
MIM 607664
UniProt P15586  
 GNS identified in exosomes derived from the following tissue/cell type
Ovarian cancer cells 23333927    
Ovarian cancer cells 23333927    
 Gene ontology annotations for GNS
Molecular Function
    protein binding GO:0005515 IPI
    sulfuric ester hydrolase activity GO:0008484 IDA
    N-acetylglucosamine-6-sulfatase activity GO:0008449 IEA
    metal ion binding GO:0046872 IEA
Biological Process
    carbohydrate metabolic process GO:0005975 TAS
    keratan sulfate catabolic process GO:0042340 TAS
    glycosaminoglycan catabolic process GO:0006027 TAS
    small molecule metabolic process GO:0044281 TAS
    glycosaminoglycan metabolic process GO:0030203 TAS
    keratan sulfate metabolic process GO:0042339 TAS
Subcellular Localization
    extracellular exosome GO:0070062 IDA
    lysosomal lumen GO:0043202 TAS
 Experiment description of studies that identified GNS in exosomes
1
Experiment ID 211
ISEV standards
✔
EM
EV Biophysical techniques
✔
TSG101|Alix
EV Cytosolic markers
✔
EpCAM|TFRC
EV Membrane markers
✔
cytochrome c|GOLGA2
EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K.
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name IGROV1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
2
Experiment ID 212
ISEV standards
✔
CEM
EV Biophysical techniques
✔
TSG101|Alix
EV Cytosolic markers
✔
EpCAM|TFRC
EV Membrane markers
✔
Cytochrome C|GOLGA2
EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K.
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name OVCAR-3
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for GNS
  Protein Interactor ExoCarta ID Identification method PubMed Species
No interactions are found.
 Pathways in which GNS is involved
PathwayEvidenceSource
Keratan sulfate degradation TAS Reactome
Lysosome Vesicle Biogenesis TAS Reactome
MPS IIID - Sanfilippo syndrome D TAS Reactome





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