ExoCarta: Gene summary

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Gene description for GNS
Gene name	glucosamine (N-acetyl)-6-sulfatase
Gene symbol	GNS
Other names/aliases	G6S
Species	Homo sapiens
Database cross references - GNS
ExoCarta	ExoCarta_2799
Vesiclepedia	VP_2799
Entrez Gene	2799
HGNC	4422
MIM	607664
UniProt	P15586
GNS identified in exosomes derived from the following tissue/cell type
Bone marrow mesenchymal stem cells	36408942
Bone marrow mesenchymal stem cells	36408942
Bone marrow mesenchymal stem cells	36408942
Bone marrow mesenchymal stem cells	36408942
Chondrocytes	35931686
Chondrocytes	35931686
Chondrocytes	35931686
Chondrocytes	35931686
Mesenchymal stem cells	36408942
Ovarian cancer cells	23333927
Ovarian cancer cells	23333927
Retinal pigment epithelial cells	35333565
Retinal pigment epithelial cells	35333565

Gene ontology annotations for GNS

Molecular Function
	protein binding	GO:0005515	IPI
	glycosaminoglycan binding	GO:0005539	IBA
	N-acetylglucosamine-6-sulfatase activity	GO:0008449	IBA
	N-acetylglucosamine-6-sulfatase activity	GO:0008449	TAS
	sulfuric ester hydrolase activity	GO:0008484	IDA
	sulfate binding	GO:0043199	IEA
	metal ion binding	GO:0046872	IEA
Biological Process
	glycosaminoglycan catabolic process	GO:0006027	TAS
	keratan sulfate catabolic process	GO:0042340	TAS
Subcellular Localization
	extracellular region	GO:0005576	TAS
	azurophil granule lumen	GO:0035578	TAS
	lysosomal lumen	GO:0043202	TAS
	extracellular exosome	GO:0070062	HDA
	ficolin-1-rich granule lumen	GO:1904813	TAS

Experiment description of studies that identified GNS in exosomes

Experiment ID

489

MISEV standards
✔ EM	Biophysical techniques
✔ Cd9\|Cd81\|Cd63\|Gapdh\|Sdcbp\|Lamp1\|Aqp1\|Rab5a\|Icam1\|Cd82\|Itga2b\|Tsg101\|Lamp2\|Rab35\|Flot1\|Flot2\|Cd151\|Rab5b\|Tfrc\|Uchl1	Enriched markers
✔ Canx	Negative markers
✔ NTA	Particle analysis

Identified molecule

Protein

Identification method

Mass spectrometry

PubMed ID

36408942

Organism

Rattus norvegicus

Experiment description

Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells

Authors

"Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."

Journal name

Proteomics

Publication year

2023

Sample

Bone marrow mesenchymal stem cells

Sample name

BMSC - Passage 6

Isolation/purification methods

Differential centrifugation
Filtration
Ultracentrifugation

Flotation density

Molecules identified in the study

Protein

Methods used in the study

Western blotting
Mass spectometry

Experiment ID

490

MISEV standards
✔ EM	Biophysical techniques
✔ Cd9\|Cd81\|Cd63\|Gapdh\|Sdcbp\|Lamp1\|Aqp1\|Rab5a\|Icam1\|Cd82\|Itga2b\|Tsg101\|Lamp2\|Rab35\|Flot1\|Flot2\|Cd151\|Rab5b\|Tfrc\|Uchl1	Enriched markers
✔ Canx	Negative markers
✔ NTA	Particle analysis

Identified molecule

Protein

Identification method

Mass spectrometry

PubMed ID

36408942

Organism

Rattus norvegicus

Experiment description

Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells

Authors

"Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."

Journal name

Proteomics

Publication year

2023

Sample

Bone marrow mesenchymal stem cells

Sample name

BMSC - Passage 7

Isolation/purification methods

Differential centrifugation
Filtration
Ultracentrifugation

Flotation density

Molecules identified in the study

Protein

Methods used in the study

Western blotting
Mass spectometry

Experiment ID

491

MISEV standards
✔ EM	Biophysical techniques
✔ Cd9\|Cd81\|Cd63\|Gapdh\|Sdcbp\|Lamp1\|Aqp1\|Rab5a\|Icam1\|Cd82\|Itga2b\|Tsg101\|Lamp2\|Rab35\|Flot1\|Flot2\|Cd151\|Rab5b\|Tfrc\|Uchl1	Enriched markers
✔ Canx	Negative markers
✔ NTA	Particle analysis

Identified molecule

Protein

Identification method

Mass spectrometry

PubMed ID

36408942

Organism

Rattus norvegicus

Experiment description

Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells

Authors

"Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."

Journal name

Proteomics

Publication year

2023

Sample

Bone marrow mesenchymal stem cells

Sample name

BMSC - Passage 8

Isolation/purification methods

Differential centrifugation
Filtration
Ultracentrifugation

Flotation density

Molecules identified in the study

Protein

Methods used in the study

Western blotting
Mass spectometry

Experiment ID

492

MISEV standards
✔ EM	Biophysical techniques
✔ Cd9\|Cd81\|Cd63\|Gapdh\|Sdcbp\|Lamp1\|Aqp1\|Rab5a\|Icam1\|Cd82\|Itga2b\|Tsg101\|Lamp2\|Rab35\|Flot1\|Flot2\|Cd151\|Rab5b\|Tfrc\|Uchl1	Enriched markers
✔ Canx	Negative markers
✔ NTA	Particle analysis

Identified molecule

Protein

Identification method

Mass spectrometry

PubMed ID

36408942

Organism

Rattus norvegicus

Experiment description

Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells

Authors

"Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."

Journal name

Proteomics

Publication year

2023

Sample

Bone marrow mesenchymal stem cells

Sample name

BMSC - Passage 9

Isolation/purification methods

Differential centrifugation
Filtration
Ultracentrifugation

Flotation density

Molecules identified in the study

Protein

Methods used in the study

Western blotting
Mass spectometry

Experiment ID

494

MISEV standards
✔ EM	Biophysical techniques
✔ CD9\|CD81\|CD151\|CD63\|CD82\|FLOT1\|FLOT2\|GAPDH\|LAMP1\|LAMP2\|SDCBP\|TFRC\|TSG101	Enriched markers
✘	Negative markers
✔ NTA	Particle analysis

Identified molecule

Protein

Identification method

Mass spectrometry

PubMed ID

35931686

Organism

Homo sapiens

Experiment description

Extracellular vesicles enriched in connexin 43 promote a senescent phenotype in bone and synovial cells contributing to osteoarthritis progression

Authors

"Varela-EirÃn M, Carpintero-FernÃ¡ndez P, GuitiÃ¡n-CaamaÃ±o A, Varela-VÃ¡zquez A, GarcÃa-Yuste A, SÃ¡nchez-Temprano A, Bravo-LÃ³pez SB, YaÃ±ez-Cabanas J, Fonseca E, Largo R, Mobasheri A, Caeiro JR, MayÃ¡n MD."

Journal name

Cell Death Dis

Publication year

2022

Sample

Chondrocytes

Sample name

Osteoarthritic cartilage

Isolation/purification methods

Differential centrifugation
Filtration
Ultracentrifugation

Flotation density

Molecules identified in the study

Protein

Methods used in the study

Western blotting
Mass spectometry

Experiment ID

496

MISEV standards
✘	Biophysical techniques
✔ CD9\|CD151\|CD63\|CD81\|CD82\|FLOT1\|FLOT2\|GAPDH\|LAMP1\|LAMP2\|SDCBP\|TFRC\|TSG101	Enriched markers
✘	Negative markers
✘	Particle analysis

Identified molecule

Protein

Identification method

Mass spectrometry

PubMed ID

35931686

Organism

Homo sapiens

Experiment description

Extracellular vesicles enriched in connexin 43 promote a senescent phenotype in bone and synovial cells contributing to osteoarthritis progression

Authors

Journal name

Cell Death Dis

Publication year

2022

Sample

Chondrocytes

Sample name

Healthy cartilage

Isolation/purification methods

Differential centrifugation
Filtration
Ultracentrifugation

Flotation density

Molecules identified in the study

Protein

Methods used in the study

Western blotting
Mass spectometry

Experiment ID

497

MISEV standards
✘	Biophysical techniques
✔ CD9\|CD151\|CD63\|CD81\|CD82\|FLOT1\|FLOT2\|GAPDH\|LAMP1\|LAMP2\|RAB5A\|SDCBP\|TFRC\|TSG101\|UCHL1	Enriched markers
✘	Negative markers
✔ NTA	Particle analysis

Identified molecule

Protein

Identification method

Mass spectrometry

PubMed ID

35931686

Organism

Homo sapiens

Experiment description

Extracellular vesicles enriched in connexin 43 promote a senescent phenotype in bone and synovial cells contributing to osteoarthritis progression

Authors

Journal name

Cell Death Dis

Publication year

2022

Sample

Chondrocytes

Sample name

T/C-28a2

Isolation/purification methods

Differential centrifugation
Filtration
Ultracentrifugation

Flotation density

Molecules identified in the study

Protein

Methods used in the study

Western blotting
Mass spectometry

Experiment ID

498

MISEV standards
✔ EM	Biophysical techniques
✔ CD9\|CD151\|CD63\|CD81\|CD82\|FLOT1\|FLOT2\|GAPDH\|LAMP1\|LAMP2\|RAB5A\|SDCBP\|TFRC\|TSG101\|UCHL1	Enriched markers
✘	Negative markers
✔ NTA	Particle analysis

Identified molecule

Protein

Identification method

Mass spectrometry

PubMed ID

35931686

Organism

Homo sapiens

Experiment description

Extracellular vesicles enriched in connexin 43 promote a senescent phenotype in bone and synovial cells contributing to osteoarthritis progression

Authors

Journal name

Cell Death Dis

Publication year

2022

Sample

Chondrocytes

Sample name

T/C-28a2

Isolation/purification methods

Differential centrifugation
Filtration
Ultracentrifugation

Flotation density

Molecules identified in the study

Protein

Methods used in the study

Western blotting
Mass spectometry

Experiment ID

488

MISEV standards
✔ EM	Biophysical techniques
✔ CD9\|CD81\|CD63\|GAPDH\|SDCBP\|LAMP1\|TFRC\|UCHL1\|FLOT2\|LAMP2\|FLOT1\|ICAM1\|RAB5B\|CD151\|RAB35\|TSG101\|RAB5A\|CD82	Enriched markers
✔ CANX	Negative markers
✔ NTA	Particle analysis

Identified molecule

Protein

Identification method

Mass spectrometry

PubMed ID

36408942

Organism

Homo sapiens

Experiment description

Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells

Authors

"Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."

Journal name

Proteomics

Publication year

2023

Sample

Mesenchymal stem cells

Sample name

UCMSC

Isolation/purification methods

Differential centrifugation
Filtration
Ultracentrifugation

Flotation density

Molecules identified in the study

Protein

Methods used in the study

Western blotting
Mass spectometry

Experiment ID

211

MISEV standards
✔ EM	Biophysical techniques
✔ TSG101\|Alix\|EpCAM\|TFRC	Enriched markers
✔ cytochrome c\|GOLGA2	Negative markers
✘	Particle analysis

Identified molecule

protein

Identification method

Mass spectrometry

PubMed ID

23333927

Organism

Homo sapiens

Experiment description

Characterization and proteomic analysis of ovarian cancer-derived exosomes.

Authors

"Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K."

Journal name

J Proteomics

Publication year

2013

Sample

Ovarian cancer cells

Sample name

IGROV1

Isolation/purification methods

Differential centrifugation
Ultracentrifugation
Sucrose density gradient

Flotation density

1.09-1.15 g/mL

Molecules identified in the study

Protein

Methods used in the study

Mass spectrometry

Experiment ID

212

MISEV standards
✔ CEM	Biophysical techniques
✔ TSG101\|Alix\|EpCAM\|TFRC	Enriched markers
✔ Cytochrome C\|GOLGA2	Negative markers
✘	Particle analysis

Identified molecule

protein

Identification method

Mass spectrometry

PubMed ID

23333927

Organism

Homo sapiens

Experiment description

Characterization and proteomic analysis of ovarian cancer-derived exosomes.

Authors

"Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K."

Journal name

J Proteomics

Publication year

2013

Sample

Ovarian cancer cells

Sample name

OVCAR-3

Isolation/purification methods

Differential centrifugation
Ultracentrifugation
Sucrose density gradient

Flotation density

1.09-1.15 g/mL

Molecules identified in the study

Protein

Methods used in the study

Mass spectrometry

Experiment ID

834

MISEV standards
✔ EM	Biophysical techniques
✔ LAMP2\|CD63\|FLOT1\|ITGA2B\|ICAM1\|CD9\|CD151\|TFRC\|RAB5A\|GAPDH\|AQP1\|TSG101	Enriched markers
✔ CANX	Negative markers
✔ NTA	Particle analysis

Identified molecule

Protein

Identification method

Mass spectrometry

PubMed ID

35333565

Organism

Homo sapiens

Experiment description

LAMP2A regulates the loading of proteins into exosomes

Authors

"Ferreira JV, da Rosa Soares A, Ramalho J, MÃ¡ximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, GirÃ£o H, van Niel G, Pereira P"

Journal name

Sci Adv

Publication year

2022

Sample

Retinal pigment epithelial cells

Sample name

ARPE-19

Isolation/purification methods

Differential centrifugation
Filtration
Ultracentrifugation

Flotation density

Molecules identified in the study

Protein

Methods used in the study

Western blotting
Mass spectrometry

Experiment ID

835

MISEV standards
✔ EM	Biophysical techniques
✔ CD63\|FLOT1\|ITGA2B\|ICAM1\|CD9\|CD151\|TFRC\|RAB5A\|GAPDH\|AQP1\|TSG101	Enriched markers
✔ CANX	Negative markers
✔ NTA	Particle analysis

Identified molecule

Protein

Identification method

Mass spectrometry

PubMed ID

35333565

Organism

Homo sapiens

Experiment description

LAMP2A regulates the loading of proteins into exosomes

Authors

"Ferreira JV, da Rosa Soares A, Ramalho J, MÃ¡ximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, GirÃ£o H, van Niel G, Pereira P"

Journal name

Sci Adv

Publication year

2022

Sample

Retinal pigment epithelial cells

Sample name

ARPE-19

Isolation/purification methods

Differential centrifugation
Filtration
Ultracentrifugation

Flotation density

Molecules identified in the study

Protein

Methods used in the study

Western blotting
Mass spectrometry

Protein-protein interactions for GNS

Protein Interactor

ExoCarta ID

Identification method

PubMed

Species

NCBP1

4686

Co-fractionation

Homo sapiens

HDAC2

3066

Co-fractionation

Homo sapiens

ATG9A

79065

Proximity Label-MS

Homo sapiens

ENGASE

64772

Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens

NAPA

8775

Co-fractionation

Homo sapiens

DYNC1I2

1781

Co-fractionation

Homo sapiens

PBK

Co-fractionation

Homo sapiens

EML4

27436

Co-fractionation

Homo sapiens

RANBP3

8498

Co-fractionation

Homo sapiens

GBP2

Co-fractionation

Homo sapiens

ACBD3

64746

Co-fractionation

Homo sapiens

EFNA3

Affinity Capture-MS

Homo sapiens

RPS20

6224

Co-fractionation

Homo sapiens

SULF2

55959

Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens

EFNA4

Affinity Capture-MS

Homo sapiens

GSPT1

2935

Co-fractionation

Homo sapiens

PEPD

5184

Co-fractionation

Homo sapiens

TMEM192

201931

Affinity Capture-MS

Homo sapiens

UCHL5

51377

Co-fractionation

Homo sapiens

MECP2

4204

Affinity Capture-MS

Homo sapiens

CUL1

8454

Affinity Capture-MS

Homo sapiens

RBM8A

9939

Co-fractionation

Homo sapiens

GDA

9615

Co-fractionation

Homo sapiens

YWHAB

7529

Co-fractionation

Homo sapiens

RBBP7

5931

Co-fractionation

Homo sapiens

TTC4

Co-fractionation

Homo sapiens

MRE11A

4361

Co-fractionation

Homo sapiens

PSMD4

5710

Co-fractionation

Homo sapiens

TGM2

7052

Co-fractionation

Homo sapiens

KLC1

3831

Co-fractionation

Homo sapiens

ACIN1

22985

Co-fractionation

Homo sapiens

ACACA

Co-fractionation

Homo sapiens

UBFD1

56061

Co-fractionation

Homo sapiens

DEK

7913

Co-fractionation

Homo sapiens

TUBA1B

10376

Co-fractionation

Homo sapiens

LRRFIP1

9208

Co-fractionation

Homo sapiens

PSMD2

5708

Co-fractionation

Homo sapiens

MYH9

4627

Co-fractionation

Homo sapiens

SUMF1

285362

Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens

SMS

6611

Co-fractionation

Homo sapiens

CAPN1

823

Co-fractionation

Homo sapiens

MYL12A

10627

Co-fractionation

Homo sapiens

FGFR1OP

Affinity Capture-MS

Homo sapiens

BZW1

9689

Co-fractionation

Homo sapiens

ST13

6767

Co-fractionation

Homo sapiens

RPS6KA1

6195

Co-fractionation

Homo sapiens

IPO4

79711

Co-fractionation

Homo sapiens

GOLGA3

2802

Co-fractionation

Homo sapiens

NASP

4678

Co-fractionation

Homo sapiens

AHCYL1

10768

Co-fractionation

Homo sapiens

UGGT1

56886

Affinity Capture-MS

Homo sapiens

ASAH1

427

Co-fractionation

Homo sapiens

TRIP6

7205

Co-fractionation

Homo sapiens

MYH14

79784

Co-fractionation

Homo sapiens

PRKAR2A

5576

Co-fractionation

Homo sapiens

TUBB4B

10383

Co-fractionation

Homo sapiens

SLK

9748

Co-fractionation

Homo sapiens

FBXO6

26270

Affinity Capture-MS

Homo sapiens

BCCIP

56647

Co-fractionation

Homo sapiens

PPP6R3

55291

Co-fractionation

Homo sapiens

RBBP4

5928

Co-fractionation

Homo sapiens

Cep152

Affinity Capture-MS

Mus musculus

ELMSAN1

Co-fractionation

Homo sapiens

G6PD

2539

Co-fractionation

Homo sapiens

STRAP

11171

Co-fractionation

Homo sapiens

PRKCI

5584

Co-fractionation

Homo sapiens

NRBP1

29959

Co-fractionation

Homo sapiens

VIM

7431

Co-fractionation

Homo sapiens

FBXO2

26232

Affinity Capture-MS

Homo sapiens

PRPF4

9128

Co-fractionation

Homo sapiens

NFKB1

4790

Co-fractionation

Homo sapiens

PPP6C

5537

Co-fractionation

Homo sapiens

GLA

2717

Co-fractionation

Homo sapiens

HSPD1

3329

Affinity Capture-MS

Homo sapiens

SCLY

Co-fractionation

Homo sapiens

COL17A1

1308

Co-fractionation

Homo sapiens

MGEA5

10724

Co-fractionation

Homo sapiens

TAF6

6878

Co-fractionation

Homo sapiens

GLS

2744

Co-fractionation

Homo sapiens

KIF2A

3796

Affinity Capture-MS

Homo sapiens

CASP7

Co-fractionation

Homo sapiens

USP48

Co-fractionation

Homo sapiens

LDHAL6B

92483

Co-fractionation

Homo sapiens

CLPX

10845

Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens

MVD

4597

Co-fractionation

Homo sapiens

C11orf58

Co-fractionation

Homo sapiens

TP53

7157

Co-fractionation

Homo sapiens

RUVBL2

10856

Co-fractionation

Homo sapiens

Msn

17698

Affinity Capture-MS

Mus musculus

EFNA1

Affinity Capture-MS

Homo sapiens

DDX58

23586

Affinity Capture-RNA

Homo sapiens

FKBP14

Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens

DNAJA2

10294

Co-fractionation

Homo sapiens

PARD6B

84612

Affinity Capture-MS		Homo sapiens
Co-fractionation		Homo sapiens

CAD

790

Co-fractionation

Homo sapiens

YBX3

8531

Co-fractionation

Homo sapiens

GAPVD1

26130

Co-fractionation

Homo sapiens

PRKCA

5578

Co-fractionation

Homo sapiens

IGF2R

3482

Affinity Capture-MS

Homo sapiens

100

KYNU

Co-fractionation

Homo sapiens

101

EPHA2

1969

Proximity Label-MS

Homo sapiens

102

EIF5A

1984

Co-fractionation

Homo sapiens

103

PSMD1

5707

Co-fractionation

Homo sapiens

104

Prkaa1

Affinity Capture-MS

Mus musculus

105

PPP2R2A

5520

Co-fractionation

Homo sapiens

106

ABCE1

6059

Affinity Capture-MS

Homo sapiens

107

CPSF6

11052

Co-fractionation

Homo sapiens

108

CNDP2

55748

Co-fractionation

Homo sapiens

109

PSMD3

5709

Co-fractionation

Homo sapiens

110

CAPN2

824

Affinity Capture-MS

Homo sapiens

View the network

Pathways in which GNS is involved

Pathway	Evidence	Source
Disease	TAS	Reactome
Diseases of carbohydrate metabolism	TAS	Reactome
Diseases of metabolism	TAS	Reactome
Glycosaminoglycan metabolism	TAS	Reactome
Immune System	TAS	Reactome
Innate Immune System	TAS	Reactome
Keratan sulfate degradation	TAS	Reactome
Keratan sulfate/keratin metabolism	TAS	Reactome
Lysosome Vesicle Biogenesis	TAS	Reactome
Membrane Trafficking	TAS	Reactome
Metabolism	TAS	Reactome
Metabolism of carbohydrates	TAS	Reactome
MPS IIID - Sanfilippo syndrome D	TAS	Reactome
Mucopolysaccharidoses	TAS	Reactome
Neutrophil degranulation	TAS	Reactome
trans-Golgi Network Vesicle Budding	TAS	Reactome
Vesicle-mediated transport	TAS	Reactome

Perform bioinformatics analysis of your extracellular vesicle data set using FunRich, a open access standalone tool. NEW UPDATED VERSION OF FunRich available for download (12/09/2016) from here