Gene description for PLEKHA5
Gene name pleckstrin homology domain containing, family A member 5
Gene symbol PLEKHA5
Other names/aliases PEPP-2
PEPP2
Species Homo sapiens
 Database cross references - PLEKHA5
ExoCarta ExoCarta_54477
Vesiclepedia VP_54477
Entrez Gene 54477
HGNC 30036
MIM 607770
UniProt Q9HAU0  
 PLEKHA5 identified in exosomes derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Retinal pigment epithelial cells 35333565    
Retinal pigment epithelial cells 35333565    
 Gene ontology annotations for PLEKHA5
Molecular Function
    protein binding GO:0005515 IPI
    phosphatidylinositol-5-phosphate binding GO:0010314 IBA
    phosphatidylinositol-3-phosphate binding GO:0032266 IBA
    phosphatidylinositol-4-phosphate binding GO:0070273 IBA
    phosphatidylinositol-3,5-bisphosphate binding GO:0080025 IBA
Biological Process
    reproductive system development GO:0061458 IEA
Subcellular Localization
    nucleoplasm GO:0005654 IDA
    cytosol GO:0005829 IBA
    cytosol GO:0005829 IDA
    postsynaptic density GO:0014069 IEA
    membrane GO:0016020 HDA
    glutamatergic synapse GO:0098978 IEA
 Experiment description of studies that identified PLEKHA5 in exosomes
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 834
MISEV standards
✔
EM
Biophysical techniques
✔
LAMP2|CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P"
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
6
Experiment ID 835
MISEV standards
✔
EM
Biophysical techniques
✔
CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P"
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for PLEKHA5
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 GJD3  
Proximity Label-MS Homo sapiens
2 CEP250 11190
Two-hybrid Homo sapiens
3 CLIP1 6249
Proximity Label-MS Homo sapiens
4 BLOC1S6  
Two-hybrid Homo sapiens
5 OCLN 100506658
Proximity Label-MS Homo sapiens
6 KSR1  
Affinity Capture-MS Homo sapiens
7 MARCKS 4082
Proximity Label-MS Homo sapiens
8 GJA1 2697
Proximity Label-MS Homo sapiens
9 WASH2P  
Two-hybrid Homo sapiens
10 PARD3 56288
Proximity Label-MS Homo sapiens
11 ARHGEF9  
Two-hybrid Homo sapiens
12 MAP4 4134
Affinity Capture-MS Homo sapiens
13 LSM4 25804
Two-hybrid Homo sapiens
14 SRGAP2 23380
Affinity Capture-MS Homo sapiens
15 CALM1 801
Affinity Capture-MS Homo sapiens
16 CAMSAP2  
Proximity Label-MS Homo sapiens
17 CBY1  
Affinity Capture-MS Homo sapiens
18 STX4 6810
Proximity Label-MS Homo sapiens
19 CCDC92  
Two-hybrid Homo sapiens
20 EIF4E2  
Affinity Capture-MS Homo sapiens
21 KIF13B 23303
Affinity Capture-MS Homo sapiens
22 CCDC88B 283234
Two-hybrid Homo sapiens
23 MRFAP1L1  
Affinity Capture-MS Homo sapiens
24 MLLT4 4301
Proximity Label-MS Homo sapiens
25 DYRK1A 1859
Affinity Capture-MS Homo sapiens
26 ZBTB21  
Affinity Capture-MS Homo sapiens
27 SOGA1 140710
Two-hybrid Homo sapiens
28 ZNF638 27332
Affinity Capture-MS Homo sapiens
29 RAB3IP  
Affinity Capture-MS Homo sapiens
30 GINM1 116254
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
31 YWHAG 7532
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
32 KTN1 3895
Two-hybrid Homo sapiens
33 STX6 10228
Proximity Label-MS Homo sapiens
34 YWHAH 7533
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
35 LRFN1  
Affinity Capture-MS Homo sapiens
36 IL17RA 23765
Affinity Capture-MS Homo sapiens
37 RAB35 11021
Proximity Label-MS Homo sapiens
38 EBAG9 9166
Proximity Label-MS Homo sapiens
39 KIF3A 11127
Two-hybrid Homo sapiens
40 LAMP1 3916
Proximity Label-MS Homo sapiens
41 Tmed10 68581
Affinity Capture-MS Mus musculus
42 KCNE3  
Affinity Capture-MS Homo sapiens
43 YWHAB 7529
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
44 KANSL1  
Two-hybrid Homo sapiens
45 AGAP1  
Affinity Capture-MS Homo sapiens
46 PFN1 5216
Proximity Label-MS Homo sapiens
47 KRT8 3856
Proximity Label-MS Homo sapiens
48 CAMK2A 815
Affinity Capture-MS Homo sapiens
49 CEP350 9857
Two-hybrid Homo sapiens
50 CDK16 5127
Affinity Capture-MS Homo sapiens
51 MIB1 57534
Proximity Label-MS Homo sapiens
52 FHL3 2275
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
53 CXADR 1525
Proximity Label-MS Homo sapiens
54 SIPA1L1 26037
Affinity Capture-MS Homo sapiens
55 TESK2  
Affinity Capture-MS Homo sapiens
56 LYN 4067
Proximity Label-MS Homo sapiens
57 MAPRE1 22919
Proximity Label-MS Homo sapiens
58 MCAM 4162
Proximity Label-MS Homo sapiens
59 RAB4A 5867
Proximity Label-MS Homo sapiens
60 DNAJC5 80331
Proximity Label-MS Homo sapiens
61 CDH1 999
Proximity Label-MS Homo sapiens
62 DES 1674
Two-hybrid Homo sapiens
63 YWHAE 7531
Affinity Capture-MS Homo sapiens
64 DIRAS3  
Proximity Label-MS Homo sapiens
65 PNMA2  
Affinity Capture-MS Homo sapiens
66 DCTN1 1639
Proximity Label-MS Homo sapiens
67 KIF11 3832
Affinity Capture-MS Homo sapiens
68 EVPL 2125
Two-hybrid Homo sapiens
69 KCTD3  
Affinity Capture-MS Homo sapiens
70 PIPSL 266971
Affinity Capture-MS Homo sapiens
71 CALML3 810
Affinity Capture-MS Homo sapiens
72 PHLPP1  
Proximity Label-MS Homo sapiens
73 LIMA1 51474
Affinity Capture-MS Homo sapiens
74 FTL 2512
Affinity Capture-MS Homo sapiens
75 DCLK1 9201
Affinity Capture-MS Homo sapiens
76 CIAO1 9391
Affinity Capture-MS Homo sapiens
77 XPO1 7514
Affinity Capture-MS Homo sapiens
78 RC3H2  
Affinity Capture-MS Homo sapiens
79 CDC25B 994
Affinity Capture-MS Homo sapiens
80 CCDC186 55088
Two-hybrid Homo sapiens
81 LAMB2 3913
Two-hybrid Homo sapiens
82 EPB41L4A 64097
Proximity Label-MS Homo sapiens
83 CTNNB1 1499
Proximity Label-MS Homo sapiens
84 YWHAZ 7534
Affinity Capture-MS Homo sapiens
85 ARF6 382
Proximity Label-MS Homo sapiens
86 SPTBN1 6711
Two-hybrid Homo sapiens
87 SYDE1 85360
Affinity Capture-MS Homo sapiens
88 PLEKHA5 54477
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
89 AURKB 9212
Affinity Capture-MS Homo sapiens
90 MAPRE3  
Proximity Label-MS Homo sapiens
91 PRKACA 5566
Proximity Label-MS Homo sapiens
92 ABTB2 25841
Affinity Capture-MS Homo sapiens
93 PPP2R1A 5518
Affinity Capture-MS Homo sapiens
94 GIGYF1  
Affinity Capture-MS Homo sapiens
95 SEC16A 9919
Affinity Capture-MS Homo sapiens
96 RAB9A 9367
Proximity Label-MS Homo sapiens
97 RHOB 388
Proximity Label-MS Homo sapiens
98 CLTC 1213
Affinity Capture-MS Homo sapiens
99 CTNNA1 1495
Proximity Label-MS Homo sapiens
100 PPP2R5A 5525
Affinity Capture-MS Homo sapiens
101 KRT19 3880
Two-hybrid Homo sapiens
102 FHL2 2274
Affinity Capture-MS Homo sapiens
103 PPP2CB 5516
Affinity Capture-MS Homo sapiens
104 FLOT1 10211
Proximity Label-MS Homo sapiens
105 HIF1A 3091
Affinity Capture-MS Homo sapiens
106 DENND6A 201627
Proximity Label-MS Homo sapiens
107 RNF43  
Proximity Label-MS Homo sapiens
108 KIF2A 3796
Proximity Label-MS Homo sapiens
109 PLEKHA4 57664
Affinity Capture-MS Homo sapiens
110 PROM1 8842
Two-hybrid Homo sapiens
111 SPTAN1 6709
Two-hybrid Homo sapiens
112 PLEKHA6 22874
Two-hybrid Homo sapiens
113 LAMTOR1 55004
Proximity Label-MS Homo sapiens
114 FBXL6  
Proximity Label-MS Homo sapiens
115 MAX  
Two-hybrid Homo sapiens
116 RTKN 6242
Affinity Capture-MS Homo sapiens
117 DENND1A 57706
Affinity Capture-MS Homo sapiens
118 PPM1H  
Affinity Capture-MS Homo sapiens
119 PLEC 5339
Two-hybrid Homo sapiens
120 EZR 7430
Proximity Label-MS Homo sapiens
121 SRSF12  
Affinity Capture-MS Homo sapiens
122 LCK 3932
Proximity Label-MS Homo sapiens
123 SH3BP5L  
Two-hybrid Homo sapiens
124 LATS2 26524
Proximity Label-MS Homo sapiens
125 ELAVL1 1994
Affinity Capture-RNA Homo sapiens
126 C11orf52 91894
Proximity Label-MS Homo sapiens
127 LATS1  
Proximity Label-MS Homo sapiens
128 TRIM52  
Affinity Capture-MS Homo sapiens
129 PDZD11 51248
Two-hybrid Homo sapiens
130 PPP1R13B  
Two-hybrid Homo sapiens
131 KRT20 54474
Two-hybrid Homo sapiens
132 EXTL3 2137
Two-hybrid Homo sapiens
133 UPF1 5976
Two-hybrid Homo sapiens
134 RAB11A 8766
Proximity Label-MS Homo sapiens
135 SSSCA1 10534
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
136 EPHA2 1969
Proximity Label-MS Homo sapiens
137 KRAS 3845
Proximity Label-MS Homo sapiens
138 SH3KBP1 30011
Affinity Capture-MS Homo sapiens
139 MAST3  
Affinity Capture-MS Homo sapiens
140 MAGI1  
Affinity Capture-MS Homo sapiens
141 CAV1 857
Proximity Label-MS Homo sapiens
142 SH3PXD2A 9644
Affinity Capture-MS Homo sapiens
View the network image/svg+xml



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