Gene description for KRT20
Gene name keratin 20, type I
Gene symbol KRT20
Other names/aliases CD20
CK-20
CK20
K20
KRT21
Species Homo sapiens
 Database cross references - KRT20
ExoCarta ExoCarta_54474
Vesiclepedia VP_54474
Entrez Gene 54474
HGNC 20412
MIM 608218
UniProt P35900  
 KRT20 identified in exosomes derived from the following tissue/cell type
Colorectal cancer cells 17956143    
Colorectal cancer cells 34887515    
Neuroblastoma cells 25944692    
 Gene ontology annotations for KRT20
Molecular Function
    protein binding GO:0005515 IPI
    structural constituent of cytoskeleton GO:0005200 NAS
Biological Process
    regulation of protein secretion GO:0050708 IEA
    intermediate filament organization GO:0045109 IMP
    apoptotic process GO:0006915 IEA
    cellular response to stress GO:0033554 IEA
Subcellular Localization
    intermediate filament GO:0005882 NAS
    cytoplasm GO:0005737 IDA
 Experiment description of studies that identified KRT20 in exosomes
1
Experiment ID 20
MISEV standards
EM
EV Biophysical techniques
HSP90|CD63|CD81|LAMP1
EV Enriched markers
GOLGA2|cytochrome c
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 17956143    
Organism Homo sapiens
Experiment description Proteomic analysis of microvesicles derived from human colorectal cancer cells.
Authors Choi DS, Lee JM, Park GW, Lim HW, Bang JY, Kim YK, Kwon KH, Kwon HJ, Kim KP, Gho YS
Journal name JPR
Publication year 2007
Sample Colorectal cancer cells
Sample name HT29
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Diafiltration
Flotation density 1.16 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [LTQ]
Western blotting
2
Experiment ID 1203
MISEV standards
EM
EV Biophysical techniques
SDCBP|FLOT1|CD9|CD81|CD63|EPCAM|GAPDH|LAMP1|TFRC|CD151|CD82|LAMP2|RAB35|TSG101|FLOT2|RAB5B|ICAM1|RAB5A
EV Enriched markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 34887515    
Organism Homo sapiens
Experiment description Supermeres are functional extracellular nanoparticles replete with disease biomarkers and therapeutic targets
Authors Zhang Q, Jeppesen DK, Higginbotham JN, Graves-Deal R, Trinh VQ, Ramirez MA, Sohn Y, Neininger AC, Taneja N, McKinley ET, Niitsu H, Cao Z, Evans R, Glass SE, Ray KC, Fissell WH, Hill S, Rose KL, Huh WJ, Washington MK, Ayers GD, Burnette DT, Sharma S, Rome LH, Franklin JL, Lee YA, Liu Q, Coffey RJ.
Journal name Nat Cell Biol
Publication year 2021
Sample Colorectal cancer cells
Sample name DiFi
Isolation/purification methods Differential centrifugation
Filtration
Centrifugal ultrafiltration
Ultracentrifugation
OptiPrep density gradient centrifugation
Flotation density -
Molecules identified in the study Protein
miRNA
Methods used in the study Western blotting
Mass spectrometry
RNA sequencing
3
Experiment ID 224
MISEV standards
EM|AFM
EV Biophysical techniques
Alix|TSG101|CD63|CD81
EV Enriched markers
GOLGA2
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25944692    
Organism Homo sapiens
Experiment description Proteogenomic analysis reveals exosomes are more oncogenic than ectosomes
Authors Keerthikumar S, Gangoda L, Liem M, Fonseka P, Atukorala I, Ozcitti C, Mechler A, Adda CG, Ang CS, Mathivanan S
Journal name Oncotarget
Publication year 2015
Sample Neuroblastoma cells
Sample name SH-SY5Y
Isolation/purification methods Differential centrifugation
Ultracentrifugation
OptiPrep density gradient
Flotation density 1.10 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
Western blotting
 Protein-protein interactions for KRT20
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 C14orf140  
Two-hybrid Homo sapiens
2 DIPA  
Two-hybrid Homo sapiens
3 PRC1 9055
Two-hybrid Homo sapiens
4 KRT15 3866
Two-hybrid Homo sapiens
5 FLJ11806  
Two-hybrid Homo sapiens
6 ABI2 10152
Two-hybrid Homo sapiens
7 COASY 80347
Two-hybrid Homo sapiens
8 VIM 7431
Two-hybrid Homo sapiens
9 KRT20 54474
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
10 AMOTL2 51421
Two-hybrid Homo sapiens
11 FXR2 9513
Two-hybrid Homo sapiens
12 MGC12538  
Two-hybrid Homo sapiens
13 C9orf16  
Two-hybrid Homo sapiens
14 EXOC8 149371
Two-hybrid Homo sapiens
15 EIF4E2  
Two-hybrid Homo sapiens
View the network image/svg+xml
 Pathways in which KRT20 is involved
No pathways found





Perform bioinformatics analysis of your extracellular vesicle data set using FunRich, a open access standalone tool. NEW UPDATED VERSION OF FunRich available for download (12/09/2016) from here