ExoCarta: Gene summary

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Gene description for KRT20
Gene name	keratin 20, type I
Gene symbol	KRT20
Other names/aliases	CD20 CK-20 CK20 K20 KRT21
Species	Homo sapiens
Database cross references - KRT20
ExoCarta	ExoCarta_54474
Vesiclepedia	VP_54474
Entrez Gene	54474
HGNC	20412
MIM	608218
UniProt	P35900
KRT20 identified in exosomes derived from the following tissue/cell type
Colorectal cancer cells	17956143
Colorectal cancer cells	34887515
Neuroblastoma cells	25944692

Gene ontology annotations for KRT20

Molecular Function
	structural constituent of cytoskeleton	GO:0005200	NAS
	protein binding	GO:0005515	IPI
Biological Process
	apoptotic process	GO:0006915	IEA
	cellular response to starvation	GO:0009267	IEA
	epithelial cell differentiation	GO:0030855	IBA
	intermediate filament organization	GO:0045109	IBA
	intermediate filament organization	GO:0045109	IMP
	regulation of protein secretion	GO:0050708	IEA
Subcellular Localization
	cytoplasm	GO:0005737	IDA
	cytosol	GO:0005829	IDA
	cytosol	GO:0005829	TAS
	cytoskeleton	GO:0005856	IBA
	intermediate filament	GO:0005882	NAS
	intermediate filament cytoskeleton	GO:0045111	IDA

Experiment description of studies that identified KRT20 in exosomes

Experiment ID

MISEV standards
✔ EM	Biophysical techniques
✔ HSP90\|CD63\|CD81\|LAMP1	Enriched markers
✔ GOLGA2\|cytochrome c	Negative markers
✘	Particle analysis

Identified molecule

protein

Identification method

Mass spectrometry

PubMed ID

17956143

Organism

Homo sapiens

Experiment description

Proteomic analysis of microvesicles derived from human colorectal cancer cells.

Authors

"Choi DS, Lee JM, Park GW, Lim HW, Bang JY, Kim YK, Kwon KH, Kwon HJ, Kim KP, Gho YS"

Journal name

JPR

Publication year

2007

Sample

Colorectal cancer cells

Sample name

HT29

Isolation/purification methods

Differential centrifugation
Sucrose density gradient
Diafiltration

Flotation density

1.16 g/mL

Molecules identified in the study

Protein

Methods used in the study

Mass spectrometry [LTQ]
Western blotting

Experiment ID

1203

MISEV standards
✔ EM	Biophysical techniques
✔ SDCBP\|FLOT1\|CD9\|CD81\|CD63\|EPCAM\|GAPDH\|LAMP1\|TFRC\|CD151\|CD82\|LAMP2\|RAB35\|TSG101\|FLOT2\|RAB5B\|ICAM1\|RAB5A	Enriched markers
✘	Negative markers
✘	Particle analysis

Identified molecule

protein

Identification method

Mass spectrometry

PubMed ID

34887515

Organism

Homo sapiens

Experiment description

Supermeres are functional extracellular nanoparticles replete with disease biomarkers and therapeutic targets

Authors

"Zhang Q, Jeppesen DK, Higginbotham JN, Graves-Deal R, Trinh VQ, Ramirez MA, Sohn Y, Neininger AC, Taneja N, McKinley ET, Niitsu H, Cao Z, Evans R, Glass SE, Ray KC, Fissell WH, Hill S, Rose KL, Huh WJ, Washington MK, Ayers GD, Burnette DT, Sharma S, Rome LH, Franklin JL, Lee YA, Liu Q, Coffey RJ."

Journal name

Nat Cell Biol

Publication year

2021

Sample

Colorectal cancer cells

Sample name

DiFi

Isolation/purification methods

Differential centrifugation
Filtration
Centrifugal ultrafiltration
Ultracentrifugation
OptiPrep density gradient centrifugation

Flotation density

Molecules identified in the study

Protein
miRNA

Methods used in the study

Western blotting
Mass spectrometry
RNA sequencing

Experiment ID

224

MISEV standards
✔ EM\|AFM	Biophysical techniques
✔ Alix\|TSG101\|CD63\|CD81	Enriched markers
✔ GOLGA2	Negative markers
✘	Particle analysis

Identified molecule

protein

Identification method

Mass spectrometry

PubMed ID

25944692

Organism

Homo sapiens

Experiment description

Proteogenomic analysis reveals exosomes are more oncogenic than ectosomes

Authors

"Keerthikumar S, Gangoda L, Liem M, Fonseka P, Atukorala I, Ozcitti C, Mechler A, Adda CG, Ang CS, Mathivanan S"

Journal name

Oncotarget

Publication year

2015

Sample

Neuroblastoma cells

Sample name

SH-SY5Y

Isolation/purification methods

Differential centrifugation
Ultracentrifugation
OptiPrep density gradient

Flotation density

1.10 g/mL

Molecules identified in the study

Protein

Methods used in the study

Mass spectrometry
Western blotting

Protein-protein interactions for KRT20

Protein Interactor

ExoCarta ID

Identification method

PubMed

Species

KRTAP10-7

Two-hybrid

Homo sapiens

KRT72

140807

Two-hybrid

Homo sapiens

KRT76

51350

Two-hybrid		Homo sapiens
Cross-Linking-MS (XL-MS)		Homo sapiens

KRT18

3875

Cross-Linking-MS (XL-MS)

Homo sapiens

NOTCH2NL

388677

Two-hybrid

Homo sapiens

ANKRD36BP1

Two-hybrid

Homo sapiens

KRTAP12-3

Two-hybrid

Homo sapiens

KRT3

3850

Cross-Linking-MS (XL-MS)		Homo sapiens
Two-hybrid		Homo sapiens

BCAS2

10286

Two-hybrid

Homo sapiens

KRT6B

3854

Cross-Linking-MS (XL-MS)

Homo sapiens

KRT84

3890

Cross-Linking-MS (XL-MS)

Homo sapiens

TXLNA

200081

Two-hybrid

Homo sapiens

EXOC8

149371

Two-hybrid

Homo sapiens

KRT6A

3853

Cross-Linking-MS (XL-MS)

Homo sapiens

ZC2HC1C

Two-hybrid

Homo sapiens

AMOTL2

51421

Two-hybrid		Homo sapiens
Two-hybrid		Homo sapiens

KRTAP10-8

Two-hybrid		Homo sapiens
Two-hybrid		Homo sapiens

NUP62

23636

Two-hybrid		Homo sapiens
Two-hybrid		Homo sapiens

ZC3H14

Two-hybrid

Homo sapiens

KRT34

Two-hybrid

Homo sapiens

COASY

80347

Two-hybrid

Homo sapiens

DES

1674

Two-hybrid

Homo sapiens

PRPH

5630

Two-hybrid

Homo sapiens

KRTAP1-1

Two-hybrid

Homo sapiens

SNAPIN

23557

Two-hybrid

Homo sapiens

KRT6C

286887

Cross-Linking-MS (XL-MS)

Homo sapiens

EIF4E2

Two-hybrid

Homo sapiens

KRT27

342574

Two-hybrid

Homo sapiens

PLEKHA5

54477

Two-hybrid

Homo sapiens

KRT79

338785

Cross-Linking-MS (XL-MS)

Homo sapiens

KRT15

3866

Two-hybrid		Homo sapiens
Two-hybrid		Homo sapiens

KRT24

192666

Two-hybrid

Homo sapiens

KRT7

3855

Cross-Linking-MS (XL-MS)

Homo sapiens

ABI2

10152

Two-hybrid		Homo sapiens
Two-hybrid		Homo sapiens

KRT20

54474

Two-hybrid		Homo sapiens
Two-hybrid		Homo sapiens

KRT80

144501

Two-hybrid		Homo sapiens
Two-hybrid		Homo sapiens

FXR2

9513

Two-hybrid

Homo sapiens

VCP

7415

Affinity Capture-MS

Homo sapiens

CYSRT1

375791

Two-hybrid

Homo sapiens

CEMIP

57214

Affinity Capture-MS

Homo sapiens

CCDC85B

Two-hybrid

Homo sapiens

KRT75

9119

Two-hybrid		Homo sapiens
Cross-Linking-MS (XL-MS)		Homo sapiens

PIK3R2

5296

Two-hybrid

Homo sapiens

PRC1

9055

Two-hybrid

Homo sapiens

KRT40

Two-hybrid

Homo sapiens

KRT13

3860

Two-hybrid

Homo sapiens

USHBP1

Two-hybrid

Homo sapiens

KRT2

3849

Cross-Linking-MS (XL-MS)

Homo sapiens

TFIP11

Two-hybrid		Homo sapiens
Two-hybrid		Homo sapiens

VIM

7431

Two-hybrid		Homo sapiens
Two-hybrid		Homo sapiens

WASH1

100287171

Two-hybrid

Homo sapiens

KRT5

3852

Cross-Linking-MS (XL-MS)

Homo sapiens

LINC00238

Two-hybrid

Homo sapiens

C9orf16

Two-hybrid		Homo sapiens
Two-hybrid		Homo sapiens
Two-hybrid		Homo sapiens

KRTAP6-3

Two-hybrid

Homo sapiens

KRTAP10-9

Two-hybrid

Homo sapiens

View the network

Pathways in which KRT20 is involved

Pathway	Evidence	Source
Developmental Biology	TAS	Reactome
Formation of the cornified envelope	TAS	Reactome
Keratinization	TAS	Reactome

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