Gene description for KRT80
Gene name keratin 80, type II
Gene symbol KRT80
Other names/aliases KB20
Species Homo sapiens
 Database cross references - KRT80
ExoCarta ExoCarta_144501
Vesiclepedia VP_144501
Entrez Gene 144501
HGNC 27056
MIM 611161
UniProt Q6KB66  
 KRT80 identified in exosomes derived from the following tissue/cell type
Chondrocytes 35931686    
Chondrocytes 35931686    
Hepatocellular carcinoma cells 26054723    
Mesenchymal stem cells Unpublished / Not applicable
Squamous carcinoma cells 20124223    
 Gene ontology annotations for KRT80
Molecular Function
    protein binding GO:0005515 IPI
    structural constituent of skin epidermis GO:0030280 IBA
Biological Process
    keratinization GO:0031424 IBA
    intermediate filament organization GO:0045109 IBA
Subcellular Localization
    cytoplasm GO:0005737 IDA
    cytosol GO:0005829 TAS
    intermediate filament GO:0005882 IDA
    desmosome GO:0030057 IDA
    keratin filament GO:0045095 IBA
    keratin filament GO:0045095 NAS
    intermediate filament cytoskeleton GO:0045111 IDA
 Experiment description of studies that identified KRT80 in exosomes
1
Experiment ID 494
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD81|CD151|CD63|CD82|FLOT1|FLOT2|GAPDH|LAMP1|LAMP2|SDCBP|TFRC|TSG101
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35931686    
Organism Homo sapiens
Experiment description Extracellular vesicles enriched in connexin 43 promote a senescent phenotype in bone and synovial cells contributing to osteoarthritis progression
Authors "Varela-Eirín M, Carpintero-Fernández P, Guitián-Caamaño A, Varela-Vázquez A, García-Yuste A, Sánchez-Temprano A, Bravo-López SB, Yañez-Cabanas J, Fonseca E, Largo R, Mobasheri A, Caeiro JR, Mayán MD."
Journal name Cell Death Dis
Publication year 2022
Sample Chondrocytes
Sample name Osteoarthritic cartilage
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 496
MISEV standards
✘ Biophysical techniques
✔
CD9|CD151|CD63|CD81|CD82|FLOT1|FLOT2|GAPDH|LAMP1|LAMP2|SDCBP|TFRC|TSG101
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35931686    
Organism Homo sapiens
Experiment description Extracellular vesicles enriched in connexin 43 promote a senescent phenotype in bone and synovial cells contributing to osteoarthritis progression
Authors "Varela-Eirín M, Carpintero-Fernández P, Guitián-Caamaño A, Varela-Vázquez A, García-Yuste A, Sánchez-Temprano A, Bravo-López SB, Yañez-Cabanas J, Fonseca E, Largo R, Mobasheri A, Caeiro JR, Mayán MD."
Journal name Cell Death Dis
Publication year 2022
Sample Chondrocytes
Sample name Healthy cartilage
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 235
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|Alix|HSC70|GAPDH
Enriched markers
✔
HSP90B1
Negative markers
✔
qNano
Particle analysis
Identified molecule mRNA
Identification method RNA Sequencing
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors "He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N"
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocellular carcinoma cells
Sample name HKCI-8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RT-PCR
RNA Sequencing
4
Experiment ID 126
MISEV standards
✘ Biophysical techniques
✔
GAPDH
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry [LTQ-FT Ultra]
PubMed ID Unpublished / Not applicable
Organism Homo sapiens
Experiment description Mesenchymal Stem Cell Exosomes: The Future MSC-based Therapy?
Authors "Ruenn Chai Lai, Ronne Wee Yeh Yeo, Soon Sim Tan, Bin Zhang, Yijun Yin, Newman Siu Kwan Sze, Andre Choo, and Sai Kiang Lim"
Journal name Mesenchymal Stem Cell Therapy
Publication year 2011
Sample Mesenchymal stem cells
Sample name huES9.E1
Isolation/purification methods HPLC
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Antobody array
Mass spectrometry
5
Experiment ID 191
MISEV standards
✘ Biophysical techniques
✔
Alix|CD81|CD9
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 20124223    
Organism Homo sapiens
Experiment description Hypoxic tumor cell modulates its microenvironment to enhance angiogenic and metastatic potential by secretion of proteins and exosomes.
Authors "Park JE, Tan HS, Datta A, Lai RC, Zhang H, Meng W, Lim SK, Sze SK."
Journal name Mol Cell Proteomics
Publication year 2010
Sample Squamous carcinoma cells
Sample name Squamous carcinoma cell (A431)
Isolation/purification methods Differential centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for KRT80
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 METTL14  
Affinity Capture-MS Homo sapiens
2 CYP17A1 1586
Affinity Capture-MS Homo sapiens
3 PARP1 142
Affinity Capture-MS Homo sapiens
4 KRT36 8689
Two-hybrid Homo sapiens
5 KRT35 3886
Two-hybrid Homo sapiens
6 KRT33B 3884
Two-hybrid Homo sapiens
7 SACM1L 22908
Affinity Capture-MS Homo sapiens
8 LCA5L  
Two-hybrid Homo sapiens
9 DDX3X 1654
Affinity Capture-MS Homo sapiens
10 KRT32  
Two-hybrid Homo sapiens
11 FBXO28  
Affinity Capture-MS Homo sapiens
12 FAM45B 55855
Affinity Capture-MS Homo sapiens
13 KRT34  
Two-hybrid Homo sapiens
14 IFT57 55081
Affinity Capture-MS Homo sapiens
15 TP53 7157
Affinity Capture-MS Homo sapiens
16 KRT28 162605
Two-hybrid Homo sapiens
17 HNRNPA1 3178
Affinity Capture-MS Homo sapiens
18 GINS3  
Two-hybrid Homo sapiens
19 KRT14 3861
Two-hybrid Homo sapiens
20 LMO2  
Two-hybrid Homo sapiens
21 KRT15 3866
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
22 KRT24 192666
Two-hybrid Homo sapiens
23 KRT20 54474
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
24 KRT38 8687
Two-hybrid Homo sapiens
25 C1orf109  
Two-hybrid Homo sapiens
26 OSBP 5007
Affinity Capture-MS Homo sapiens
27 COPS5 10987
Affinity Capture-MS Homo sapiens
28 MEOX2  
Two-hybrid Homo sapiens
29 ZUFSP 221302
Affinity Capture-MS Homo sapiens
30 RRBP1 6238
Affinity Capture-MS Homo sapiens
31 COPS3 8533
Two-hybrid Homo sapiens
32 STYX  
Affinity Capture-MS Homo sapiens
33 KRT19 3880
Two-hybrid Homo sapiens
34 DDX39B 7919
Affinity Capture-MS Homo sapiens
35 KRT16 3868
Two-hybrid Homo sapiens
36 CYLD  
Affinity Capture-MS Homo sapiens
37 CCDC8  
Affinity Capture-MS Homo sapiens
38 UCHL5 51377
Reconstituted Complex Homo sapiens
View the network image/svg+xml
 Pathways in which KRT80 is involved
PathwayEvidenceSource
Developmental Biology TAS Reactome
Formation of the cornified envelope TAS Reactome
Keratinization TAS Reactome





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