Gene description for CYP17A1
Gene name cytochrome P450, family 17, subfamily A, polypeptide 1
Gene symbol CYP17A1
Other names/aliases CPT7
CYP17
P450C17
S17AH
Species Homo sapiens
 Database cross references - CYP17A1
ExoCarta ExoCarta_1586
Vesiclepedia VP_1586
Entrez Gene 1586
HGNC 2593
MIM 609300
UniProt P05093  
 CYP17A1 identified in sEVs derived from the following tissue/cell type
Prostate cancer cells 25844599    
 Gene ontology annotations for CYP17A1
Molecular Function
    steroid 17-alpha-monooxygenase activity GO:0004508 IBA
    steroid 17-alpha-monooxygenase activity GO:0004508 IDA
    steroid 17-alpha-monooxygenase activity GO:0004508 IMP
    steroid 17-alpha-monooxygenase activity GO:0004508 TAS
    iron ion binding GO:0005506 IEA
    lyase activity GO:0016829 IEA
    oxygen binding GO:0019825 TAS
    heme binding GO:0020037 IDA
Biological Process
    steroid biosynthetic process GO:0006694 TAS
    androgen biosynthetic process GO:0006702 TAS
    glucocorticoid biosynthetic process GO:0006704 IEA
    glucocorticoid biosynthetic process GO:0006704 TAS
    sex differentiation GO:0007548 TAS
    steroid metabolic process GO:0008202 IDA
    hormone biosynthetic process GO:0042446 IBA
    hormone biosynthetic process GO:0042446 IDA
    progesterone metabolic process GO:0042448 IBA
    progesterone metabolic process GO:0042448 IDA
Subcellular Localization
    endoplasmic reticulum GO:0005783 NAS
    endoplasmic reticulum membrane GO:0005789 TAS
    axon GO:0030424 IEA
    neuronal cell body GO:0043025 IEA
 Experiment description of studies that identified CYP17A1 in sEVs
1
Experiment ID 275
MISEV standards
EM
Biophysical techniques
TSG101|Alix|RAB5A|CD9|CD82|CD63|CD81
Enriched markers
AIF
Negative markers
NTA
Particle analysis
Identified molecule protein
Identification method Western blotting
Mass spectrometry
PubMed ID 25844599    
Organism Homo sapiens
Experiment description Molecular profiling of prostate cancer derived exosomes may reveal a predictive signature for response to docetaxel.
Authors "Kharaziha P, Chioureas D, Rutishauser D, Baltatzis G, Lennartsson L, Fonseca P, Azimi A, Hultenby K, Zubarev R, Ullen A, Yachnin J, Nilsson S, Panaretakis T."
Journal name Oncotarget
Publication year 2015
Sample Prostate cancer cells
Sample name DU145 - Docetaxel sensitive
Isolation/purification methods Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.12-1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry/Flow cytometry/Western blotting
 Protein-protein interactions for CYP17A1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 PPP2R4 5524
Affinity Capture-Western Homo sapiens
2 RPAIN  
Two-hybrid Homo sapiens
3 BARX1  
Two-hybrid Homo sapiens
4 CDO1  
Two-hybrid Homo sapiens
5 UBA52 7311
Affinity Capture-MS Homo sapiens
6 HSP90AB1 3326
Two-hybrid Homo sapiens
7 KRT80 144501
Affinity Capture-MS Homo sapiens
8 ATP5E 514
Two-hybrid Homo sapiens
9 TIGD7  
Two-hybrid Homo sapiens
View the network image/svg+xml



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