Gene description for KRT3
Gene name keratin 3, type II
Gene symbol KRT3
Other names/aliases CK3
K3
Species Homo sapiens
 Database cross references - KRT3
ExoCarta ExoCarta_3850
Vesiclepedia VP_3850
Entrez Gene 3850
HGNC 6440
MIM 148043
UniProt P12035  
 KRT3 identified in exosomes derived from the following tissue/cell type
Colorectal cancer cells 19837982    
Mesenchymal stem cells Unpublished / Not applicable
Neuroblastoma cells 25944692    
Retinal pigment epithelial cells 35333565    
Retinal pigment epithelial cells 35333565    
Squamous carcinoma cells 20124223    
 Gene ontology annotations for KRT3
Molecular Function
    protein binding GO:0005515 IPI
    structural constituent of skin epidermis GO:0030280 IBA
Biological Process
    epithelial cell differentiation GO:0030855 ISS
    keratinization GO:0031424 IBA
    intermediate filament cytoskeleton organization GO:0045104 IMP
    intermediate filament organization GO:0045109 IBA
Subcellular Localization
    cytosol GO:0005829 TAS
    intermediate filament GO:0005882 NAS
    keratin filament GO:0045095 IBA
    keratin filament GO:0045095 TAS
    extracellular exosome GO:0070062 HDA
 Experiment description of studies that identified KRT3 in exosomes
1
Experiment ID 21
MISEV standards
EM|IEM
Biophysical techniques
Alix|TSG101|HSP70|CD63
Enriched markers
Negative markers
Particle analysis
Identified molecule protein
Identification method
Mass spectrometry   
PubMed ID 19837982    
Organism Homo sapiens
Experiment description Proteomic and bioinformatic analysis of immunoaffinity-purified exosomes derived from the human colon tumor cell line LIM1215.
Authors "Suresh Mathivanan, Justin W.E. Lim, Bow J. Tauro, Hong Ji, Robert L. Moritz and Richard J. Simpson"
Journal name MCP
Publication year 2009
Sample Colorectal cancer cells
Sample name LIM1215
Isolation/purification methods Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.10-1.12 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [Orbitrap]
Western blotting
2
Experiment ID 126
MISEV standards
Biophysical techniques
GAPDH
Enriched markers
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry [LTQ-FT Ultra]
PubMed ID Unpublished / Not applicable
Organism Homo sapiens
Experiment description Mesenchymal Stem Cell Exosomes: The Future MSC-based Therapy?
Authors "Ruenn Chai Lai, Ronne Wee Yeh Yeo, Soon Sim Tan, Bin Zhang, Yijun Yin, Newman Siu Kwan Sze, Andre Choo, and Sai Kiang Lim"
Journal name Mesenchymal Stem Cell Therapy
Publication year 2011
Sample Mesenchymal stem cells
Sample name huES9.E1
Isolation/purification methods HPLC
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Antobody array
Mass spectrometry
3
Experiment ID 224
MISEV standards
EM|AFM
Biophysical techniques
Alix|TSG101|CD63|CD81
Enriched markers
GOLGA2
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25944692    
Organism Homo sapiens
Experiment description Proteogenomic analysis reveals exosomes are more oncogenic than ectosomes
Authors "Keerthikumar S, Gangoda L, Liem M, Fonseka P, Atukorala I, Ozcitti C, Mechler A, Adda CG, Ang CS, Mathivanan S"
Journal name Oncotarget
Publication year 2015
Sample Neuroblastoma cells
Sample name SH-SY5Y
Isolation/purification methods Differential centrifugation
Ultracentrifugation
OptiPrep density gradient
Flotation density 1.10 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
Western blotting
4
Experiment ID 834
MISEV standards
EM
Biophysical techniques
LAMP2|CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
Enriched markers
CANX
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P"
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
5
Experiment ID 835
MISEV standards
EM
Biophysical techniques
CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
Enriched markers
CANX
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P"
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
6
Experiment ID 191
MISEV standards
Biophysical techniques
Alix|CD81|CD9
Enriched markers
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 20124223    
Organism Homo sapiens
Experiment description Hypoxic tumor cell modulates its microenvironment to enhance angiogenic and metastatic potential by secretion of proteins and exosomes.
Authors "Park JE, Tan HS, Datta A, Lai RC, Zhang H, Meng W, Lim SK, Sze SK."
Journal name Mol Cell Proteomics
Publication year 2010
Sample Squamous carcinoma cells
Sample name Squamous carcinoma cell (A431)
Isolation/purification methods Differential centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for KRT3
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 SAPCD2  
Two-hybrid Homo sapiens
2 KRT26 353288
Two-hybrid Homo sapiens
3 HAUS1  
Two-hybrid Homo sapiens
4 KRT37 8688
Two-hybrid Homo sapiens
5 BPIFA1 51297
Two-hybrid Homo sapiens
6 KRT27 342574
Two-hybrid Homo sapiens
7 EPS15 2060
Affinity Capture-MS Homo sapiens
8 SIRT6  
Affinity Capture-MS Homo sapiens
9 SHC1 6464
Affinity Capture-MS Homo sapiens
10 KRT36 8689
Two-hybrid Homo sapiens
11 KRT25 147183
Two-hybrid Homo sapiens
12 KRT33B 3884
Two-hybrid Homo sapiens
13 AP2M1 1173
Affinity Capture-MS Homo sapiens
14 DES 1674
Cross-Linking-MS (XL-MS) Homo sapiens
15 KIFC3 3801
Two-hybrid Homo sapiens
16 INPPL1 3636
Affinity Capture-MS Homo sapiens
17 KRT6A 3853
Cross-Linking-MS (XL-MS) Homo sapiens
18 KRT35 3886
Two-hybrid Homo sapiens
19 CRK 1398
Affinity Capture-MS Homo sapiens
20 NUP62 23636
Two-hybrid Homo sapiens
21 WASH1 100287171
Two-hybrid Homo sapiens
22 KRT34  
Two-hybrid Homo sapiens
23 KRT75 9119
Two-hybrid Homo sapiens
Cross-Linking-MS (XL-MS) Homo sapiens
24 CUL5 8065
Affinity Capture-MS Homo sapiens
25 EPPK1 83481
Affinity Capture-MS Homo sapiens
26 TRAF6 7189
Affinity Capture-MS Homo sapiens
27 PTEN 5728
Affinity Capture-MS Homo sapiens
28 NHLRC2 374354
Affinity Capture-MS Homo sapiens
29 PRPH 5630
Cross-Linking-MS (XL-MS) Homo sapiens
30 EGFR 1956
Affinity Capture-MS Homo sapiens
31 KRT28 162605
Two-hybrid Homo sapiens
32 WWOX 51741
Two-hybrid Homo sapiens
33 KRTAP13-2  
Two-hybrid Homo sapiens
34 LINC00521  
Two-hybrid Homo sapiens
35 ZNF426  
Two-hybrid Homo sapiens
36 TRIM54  
Two-hybrid Homo sapiens
37 CBL 867
Affinity Capture-MS Homo sapiens
38 KRT14 3861
Co-fractionation Homo sapiens
Two-hybrid Homo sapiens
39 ITGAV 3685
Co-fractionation Homo sapiens
40 KRT39  
Two-hybrid Homo sapiens
41 KRT24 192666
Two-hybrid Homo sapiens
42 PLEKHG4  
Two-hybrid Homo sapiens
43 KRT84 3890
Cross-Linking-MS (XL-MS) Homo sapiens
44 HP1BP3 50809
Co-fractionation Homo sapiens
45 KRT20 54474
Two-hybrid Homo sapiens
Cross-Linking-MS (XL-MS) Homo sapiens
46 KRT38 8687
Two-hybrid Homo sapiens
47 KRT12 3859
Cross-Linking-MS (XL-MS) Homo sapiens
48 LHX3  
Two-hybrid Homo sapiens
49 TRIM27  
Two-hybrid Homo sapiens
50 C17orf70  
Affinity Capture-MS Homo sapiens
51 COPS5 10987
Affinity Capture-MS Homo sapiens
52 HYI 81888
Two-hybrid Homo sapiens
53 HGS 9146
Two-hybrid Homo sapiens
54 SKA3  
Affinity Capture-MS Homo sapiens
55 HNRNPU 3192
Co-fractionation Homo sapiens
56 AR 367
Affinity Capture-MS Homo sapiens
57 OIP5  
Two-hybrid Homo sapiens
58 CCDC67  
Two-hybrid Homo sapiens
59 RNF208  
Affinity Capture-MS Homo sapiens
60 VIM 7431
Cross-Linking-MS (XL-MS) Homo sapiens
61 KRT19 3880
Two-hybrid Homo sapiens
Cross-Linking-MS (XL-MS) Homo sapiens
62 KRT5 3852
Co-fractionation Homo sapiens
Cross-Linking-MS (XL-MS) Homo sapiens
63 C3orf62  
Two-hybrid Homo sapiens
64 LURAP1  
Two-hybrid Homo sapiens
65 KRT16 3868
Cross-Linking-MS (XL-MS) Homo sapiens
Two-hybrid Homo sapiens
66 Ncbp2  
Affinity Capture-MS Mus musculus
67 APOA1 335
Cross-Linking-MS (XL-MS) Homo sapiens
View the network image/svg+xml
 Pathways in which KRT3 is involved
PathwayEvidenceSource
Developmental Biology TAS Reactome
Formation of the cornified envelope TAS Reactome
Keratinization TAS Reactome





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