Gene ontology annotations for KRT3
Experiment description of studies that identified KRT3 in exosomes
1
Experiment ID
21
MISEV standards
✔
EM|IEM
Biophysical techniques
✔
Alix|TSG101|HSP70|CD63
Enriched markers
✘
Negative markers
✘
Particle analysis
Identified molecule
protein
Identification method
PubMed ID
19837982
Organism
Homo sapiens
Experiment description
Proteomic and bioinformatic analysis of immunoaffinity-purified exosomes derived from the human colon tumor cell line LIM1215.
Authors
"Suresh Mathivanan, Justin W.E. Lim, Bow J. Tauro, Hong Ji, Robert L. Moritz and Richard J. Simpson"
Journal name
MCP
Publication year
2009
Sample
Colorectal cancer cells
Sample name
LIM1215
Isolation/purification methods
Filtration Ultracentrifugation Sucrose density gradient
Flotation density
1.10-1.12 g/mL
Molecules identified in the study
Protein
Methods used in the study
Mass spectrometry [Orbitrap] Western blotting
2
Experiment ID
126
MISEV standards
✘
Biophysical techniques
✔
GAPDH
Enriched markers
✘
Negative markers
✘
Particle analysis
Identified molecule
protein
Identification method
Mass spectrometry [LTQ-FT Ultra]
PubMed ID
Unpublished / Not applicable
Organism
Homo sapiens
Experiment description
Mesenchymal Stem Cell Exosomes: The Future MSC-based Therapy?
Authors
"Ruenn Chai Lai, Ronne Wee Yeh Yeo, Soon Sim Tan, Bin Zhang, Yijun Yin, Newman Siu Kwan Sze, Andre Choo, and Sai Kiang Lim"
Journal name
Mesenchymal Stem Cell Therapy
Publication year
2011
Sample
Mesenchymal stem cells
Sample name
huES9.E1
Isolation/purification methods
HPLC
Flotation density
-
Molecules identified in the study
Protein
Methods used in the study
Western blotting Antobody array Mass spectrometry
3
Experiment ID
224
MISEV standards
✔
EM|AFM
Biophysical techniques
✔
Alix|TSG101|CD63|CD81
Enriched markers
✔
GOLGA2
Negative markers
✘
Particle analysis
Identified molecule
protein
Identification method
Mass spectrometry
PubMed ID
25944692
Organism
Homo sapiens
Experiment description
Proteogenomic analysis reveals exosomes are more oncogenic than ectosomes
Authors
"Keerthikumar S, Gangoda L, Liem M, Fonseka P, Atukorala I, Ozcitti C, Mechler A, Adda CG, Ang CS, Mathivanan S"
Journal name
Oncotarget
Publication year
2015
Sample
Neuroblastoma cells
Sample name
SH-SY5Y
Isolation/purification methods
Differential centrifugation Ultracentrifugation OptiPrep density gradient
Flotation density
1.10 g/mL
Molecules identified in the study
Protein
Methods used in the study
Mass spectrometry Western blotting
4
Experiment ID
834
MISEV standards
✔
EM
Biophysical techniques
✔
LAMP2|CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule
Protein
Identification method
Mass spectrometry
PubMed ID
35333565
Organism
Homo sapiens
Experiment description
LAMP2A regulates the loading of proteins into exosomes
Authors
"Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P"
Journal name
Sci Adv
Publication year
2022
Sample
Retinal pigment epithelial cells
Sample name
ARPE-19
Isolation/purification methods
Differential centrifugation Filtration Ultracentrifugation
Flotation density
-
Molecules identified in the study
Protein
Methods used in the study
Western blotting Mass spectrometry
5
Experiment ID
835
MISEV standards
✔
EM
Biophysical techniques
✔
CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule
Protein
Identification method
Mass spectrometry
PubMed ID
35333565
Organism
Homo sapiens
Experiment description
LAMP2A regulates the loading of proteins into exosomes
Authors
"Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P"
Journal name
Sci Adv
Publication year
2022
Sample
Retinal pigment epithelial cells
Sample name
ARPE-19
Isolation/purification methods
Differential centrifugation Filtration Ultracentrifugation
Flotation density
-
Molecules identified in the study
Protein
Methods used in the study
Western blotting Mass spectrometry
6
Experiment ID
191
MISEV standards
✘
Biophysical techniques
✔
Alix|CD81|CD9
Enriched markers
✘
Negative markers
✘
Particle analysis
Identified molecule
protein
Identification method
Mass spectrometry
PubMed ID
20124223
Organism
Homo sapiens
Experiment description
Hypoxic tumor cell modulates its microenvironment to enhance angiogenic and metastatic potential by secretion of proteins and exosomes.
Authors
"Park JE, Tan HS, Datta A, Lai RC, Zhang H, Meng W, Lim SK, Sze SK."
Journal name
Mol Cell Proteomics
Publication year
2010
Sample
Squamous carcinoma cells
Sample name
Squamous carcinoma cell (A431)
Isolation/purification methods
Differential centrifugation
Flotation density
-
Molecules identified in the study
Protein
Methods used in the study
Mass spectrometry
Protein-protein interactions for KRT3
Protein Interactor
ExoCarta ID
Identification method
PubMed
Species
1
SAPCD2
Two-hybrid
Homo sapiens
2
KRT26
353288
Two-hybrid
Homo sapiens
3
HAUS1
Two-hybrid
Homo sapiens
4
KRT37
8688
Two-hybrid
Homo sapiens
5
BPIFA1
51297
Two-hybrid
Homo sapiens
6
KRT27
342574
Two-hybrid
Homo sapiens
7
EPS15
2060
Affinity Capture-MS
Homo sapiens
8
SIRT6
Affinity Capture-MS
Homo sapiens
9
SHC1
6464
Affinity Capture-MS
Homo sapiens
10
KRT36
8689
Two-hybrid
Homo sapiens
11
KRT25
147183
Two-hybrid
Homo sapiens
12
KRT33B
3884
Two-hybrid
Homo sapiens
13
AP2M1
1173
Affinity Capture-MS
Homo sapiens
14
DES
1674
Cross-Linking-MS (XL-MS)
Homo sapiens
15
KIFC3
3801
Two-hybrid
Homo sapiens
16
INPPL1
3636
Affinity Capture-MS
Homo sapiens
17
KRT6A
3853
Cross-Linking-MS (XL-MS)
Homo sapiens
18
KRT35
3886
Two-hybrid
Homo sapiens
19
CRK
1398
Affinity Capture-MS
Homo sapiens
20
NUP62
23636
Two-hybrid
Homo sapiens
21
WASH1
100287171
Two-hybrid
Homo sapiens
22
KRT34
Two-hybrid
Homo sapiens
23
KRT75
9119
Two-hybrid
Homo sapiens
Cross-Linking-MS (XL-MS)
Homo sapiens
24
CUL5
8065
Affinity Capture-MS
Homo sapiens
25
EPPK1
83481
Affinity Capture-MS
Homo sapiens
26
TRAF6
7189
Affinity Capture-MS
Homo sapiens
27
PTEN
5728
Affinity Capture-MS
Homo sapiens
28
NHLRC2
374354
Affinity Capture-MS
Homo sapiens
29
PRPH
5630
Cross-Linking-MS (XL-MS)
Homo sapiens
30
EGFR
1956
Affinity Capture-MS
Homo sapiens
31
KRT28
162605
Two-hybrid
Homo sapiens
32
WWOX
51741
Two-hybrid
Homo sapiens
33
KRTAP13-2
Two-hybrid
Homo sapiens
34
LINC00521
Two-hybrid
Homo sapiens
35
ZNF426
Two-hybrid
Homo sapiens
36
TRIM54
Two-hybrid
Homo sapiens
37
CBL
867
Affinity Capture-MS
Homo sapiens
38
KRT14
3861
Co-fractionation
Homo sapiens
Two-hybrid
Homo sapiens
39
ITGAV
3685
Co-fractionation
Homo sapiens
40
KRT39
Two-hybrid
Homo sapiens
41
KRT24
192666
Two-hybrid
Homo sapiens
42
PLEKHG4
Two-hybrid
Homo sapiens
43
KRT84
3890
Cross-Linking-MS (XL-MS)
Homo sapiens
44
HP1BP3
50809
Co-fractionation
Homo sapiens
45
KRT20
54474
Two-hybrid
Homo sapiens
Cross-Linking-MS (XL-MS)
Homo sapiens
46
KRT38
8687
Two-hybrid
Homo sapiens
47
KRT12
3859
Cross-Linking-MS (XL-MS)
Homo sapiens
48
LHX3
Two-hybrid
Homo sapiens
49
TRIM27
Two-hybrid
Homo sapiens
50
C17orf70
Affinity Capture-MS
Homo sapiens
51
COPS5
10987
Affinity Capture-MS
Homo sapiens
52
HYI
81888
Two-hybrid
Homo sapiens
53
HGS
9146
Two-hybrid
Homo sapiens
54
SKA3
Affinity Capture-MS
Homo sapiens
55
HNRNPU
3192
Co-fractionation
Homo sapiens
56
AR
367
Affinity Capture-MS
Homo sapiens
57
OIP5
Two-hybrid
Homo sapiens
58
CCDC67
Two-hybrid
Homo sapiens
59
RNF208
Affinity Capture-MS
Homo sapiens
60
VIM
7431
Cross-Linking-MS (XL-MS)
Homo sapiens
61
KRT19
3880
Two-hybrid
Homo sapiens
Cross-Linking-MS (XL-MS)
Homo sapiens
62
KRT5
3852
Co-fractionation
Homo sapiens
Cross-Linking-MS (XL-MS)
Homo sapiens
63
C3orf62
Two-hybrid
Homo sapiens
64
LURAP1
Two-hybrid
Homo sapiens
65
KRT16
3868
Cross-Linking-MS (XL-MS)
Homo sapiens
Two-hybrid
Homo sapiens
66
Ncbp2
Affinity Capture-MS
Mus musculus
67
APOA1
335
Cross-Linking-MS (XL-MS)
Homo sapiens
View the network
image/svg+xml
Pathways in which KRT3 is involved