Gene description for KRT12
Gene name keratin 12, type I
Gene symbol KRT12
Other names/aliases K12
Species Homo sapiens
 Database cross references - KRT12
ExoCarta ExoCarta_3859
Vesiclepedia VP_3859
Entrez Gene 3859
HGNC 6414
MIM 601687
UniProt Q99456  
 KRT12 identified in sEVs derived from the following tissue/cell type
Neuroblastoma cells 25944692    
Saliva 19199708    
Squamous carcinoma cells 20124223    
Urine 21595033    
Urine 21595033    
Urine 21595033    
 Gene ontology annotations for KRT12
Molecular Function
    structural molecule activity GO:0005198 IEA
Biological Process
    morphogenesis of an epithelium GO:0002009 IBA
    morphogenesis of an epithelium GO:0002009 IMP
    visual perception GO:0007601 TAS
    epithelial cell differentiation GO:0030855 IBA
    intermediate filament organization GO:0045109 IBA
    cornea development in camera-type eye GO:0061303 IMP
Subcellular Localization
    cytosol GO:0005829 TAS
    cytoskeleton GO:0005856 IBA
    intermediate filament GO:0005882 IEA
    extracellular exosome GO:0070062 HDA
 Experiment description of studies that identified KRT12 in sEVs
1
Experiment ID 224
MISEV standards
✔
EM|AFM
Biophysical techniques
✔
Alix|TSG101|CD63|CD81
Enriched markers
✔
GOLGA2
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25944692    
Organism Homo sapiens
Experiment description Proteogenomic analysis reveals exosomes are more oncogenic than ectosomes
Authors "Keerthikumar S, Gangoda L, Liem M, Fonseka P, Atukorala I, Ozcitti C, Mechler A, Adda CG, Ang CS, Mathivanan S"
Journal name Oncotarget
Publication year 2015
Sample Neuroblastoma cells
Sample name SH-SY5Y
Isolation/purification methods Differential centrifugation
Ultracentrifugation
OptiPrep density gradient
Flotation density 1.10 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
Western blotting
2
Experiment ID 66
MISEV standards
✔
IEM
Biophysical techniques
✔
TSG101|Alix|CD63|CD81
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 19199708    
Organism Homo sapiens
Experiment description Proteomic analysis of human parotid gland exosomes by multidimensional protein identification technology (MudPIT).
Authors "Gonzalez-Begne M, Lu B, Han X, Hagen FK, Hand AR, Melvin JE, Yates JR"
Journal name JPR
Publication year 2009
Sample Saliva
Sample name Saliva
Isolation/purification methods Differential centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [LTQ]
Western blotting
Immunoelectron Microscopy
3
Experiment ID 191
MISEV standards
✘ Biophysical techniques
✔
Alix|CD81|CD9
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 20124223    
Organism Homo sapiens
Experiment description Hypoxic tumor cell modulates its microenvironment to enhance angiogenic and metastatic potential by secretion of proteins and exosomes.
Authors "Park JE, Tan HS, Datta A, Lai RC, Zhang H, Meng W, Lim SK, Sze SK."
Journal name Mol Cell Proteomics
Publication year 2010
Sample Squamous carcinoma cells
Sample name Squamous carcinoma cell (A431)
Isolation/purification methods Differential centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
4
Experiment ID 193
MISEV standards
✔
EM
Biophysical techniques
✔
CD63|CD9
Enriched markers
✔
PHB
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 21595033    
Organism Homo sapiens
Experiment description Proteomic analysis of urinary exosomes from patients of early IgA nephropathy and thin basement membrane nephropathy.
Authors "Moon PG, Lee JE, You S, Kim TK, Cho JH, Kim IS, Kwon TH, Kim CD, Park SH, Hwang D, Kim YL, Baek MC."
Journal name Proteomics
Publication year 2011
Sample Urine
Sample name Urine - Normal
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
5
Experiment ID 194
MISEV standards
✘ Biophysical techniques
✘ Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 21595033    
Organism Homo sapiens
Experiment description Proteomic analysis of urinary exosomes from patients of early IgA nephropathy and thin basement membrane nephropathy.
Authors "Moon PG, Lee JE, You S, Kim TK, Cho JH, Kim IS, Kwon TH, Kim CD, Park SH, Hwang D, Kim YL, Baek MC."
Journal name Proteomics
Publication year 2011
Sample Urine
Sample name Urine - Patients of basement membrane nephropathy
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
6
Experiment ID 195
MISEV standards
✘ Biophysical techniques
✘ Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 21595033    
Organism Homo sapiens
Experiment description Proteomic analysis of urinary exosomes from patients of early IgA nephropathy and thin basement membrane nephropathy.
Authors "Moon PG, Lee JE, You S, Kim TK, Cho JH, Kim IS, Kwon TH, Kim CD, Park SH, Hwang D, Kim YL, Baek MC."
Journal name Proteomics
Publication year 2011
Sample Urine
Sample name Urine - Patients of early IgA nephropathy
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for KRT12
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 KRT6A 3853
Cross-Linking-MS (XL-MS) Homo sapiens
2 KRT76 51350
Cross-Linking-MS (XL-MS) Homo sapiens
3 KRT8 3856
Cross-Linking-MS (XL-MS) Homo sapiens
4 CRK 1398
Affinity Capture-MS Homo sapiens
5 PRPH 5630
Cross-Linking-MS (XL-MS) Homo sapiens
6 KRT2 3849
Cross-Linking-MS (XL-MS) Homo sapiens
7 ALB 213
Cross-Linking-MS (XL-MS) Homo sapiens
8 TRAF6 7189
Affinity Capture-MS Homo sapiens
9 KRT3 3850
Cross-Linking-MS (XL-MS) Homo sapiens
10 DES 1674
Cross-Linking-MS (XL-MS) Homo sapiens
11 VIM 7431
Cross-Linking-MS (XL-MS) Homo sapiens
12 KRT19 3880
Cross-Linking-MS (XL-MS) Homo sapiens
13 KRT5 3852
Cross-Linking-MS (XL-MS) Homo sapiens
14 KRT6B 3854
Cross-Linking-MS (XL-MS) Homo sapiens
15 KRT7 3855
Cross-Linking-MS (XL-MS) Homo sapiens
16 CYLD  
Affinity Capture-MS Homo sapiens
17 KRT75 9119
Cross-Linking-MS (XL-MS) Homo sapiens
18 UCHL5 51377
Reconstituted Complex Homo sapiens
View the network image/svg+xml
 Pathways in which KRT12 is involved
PathwayEvidenceSource
Developmental Biology TAS Reactome
Formation of the cornified envelope TAS Reactome
Keratinization TAS Reactome





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