Gene description for PLEKHA6
Gene name pleckstrin homology domain containing, family A member 6
Gene symbol PLEKHA6
Other names/aliases PEPP-3
PEPP3
Species Homo sapiens
 Database cross references - PLEKHA6
ExoCarta ExoCarta_22874
Vesiclepedia VP_22874
Entrez Gene 22874
HGNC 17053
MIM 607771
UniProt Q9Y2H5  
 PLEKHA6 identified in exosomes derived from the following tissue/cell type
Hepatocellular carcinoma cells 26054723    
Hepatocellular carcinoma cells 26054723    
Retinal pigment epithelial cells 35333565    
Retinal pigment epithelial cells 35333565    
 Gene ontology annotations for PLEKHA6
Molecular Function
    protein binding GO:0005515 IPI
 Experiment description of studies that identified PLEKHA6 in exosomes
1
Experiment ID 234
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|Alix|HSC70|GAPDH
Enriched markers
✔
HSP90B1
Negative markers
✔
qNano
Particle analysis
Identified molecule mRNA
Identification method RNA Sequencing
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors "He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N"
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocellular carcinoma cells
Sample name HKCI-C3
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RT-PCR
RNA Sequencing
2
Experiment ID 236
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|Alix|HSC70|GAPDH
Enriched markers
✔
HSP90B1
Negative markers
✔
qNano
Particle analysis
Identified molecule mRNA
Identification method RNA Sequencing
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors "He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N"
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocellular carcinoma cells
Sample name MHCC97L
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RT-PCR
RNA Sequencing
3
Experiment ID 834
MISEV standards
✔
EM
Biophysical techniques
✔
LAMP2|CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P"
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
4
Experiment ID 835
MISEV standards
✔
EM
Biophysical techniques
✔
CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P"
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for PLEKHA6
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 HNRNPH1 3187
Affinity Capture-RNA Homo sapiens
2 MYBPHL  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
3 UBE2H 7328
Affinity Capture-MS Homo sapiens
4 HNRNPD 3184
Cross-Linking-MS (XL-MS) Homo sapiens
5 FOXA1  
Affinity Capture-MS Homo sapiens
6 PLEKHA4 57664
Affinity Capture-MS Homo sapiens
7 MYCN  
Affinity Capture-MS Homo sapiens
8 WBP5  
Affinity Capture-MS Homo sapiens
9 XPO1 7514
Affinity Capture-MS Homo sapiens
10 KCTD9  
Affinity Capture-MS Homo sapiens
11 PARD3 56288
Proximity Label-MS Homo sapiens
12 CTNNB1 1499
Affinity Capture-MS Homo sapiens
Proximity Label-MS Homo sapiens
13 UBE2Z 65264
Two-hybrid Homo sapiens
14 MED9  
Affinity Capture-MS Homo sapiens
15 ZMYND19  
Affinity Capture-MS Homo sapiens
16 PFDN1 5201
Affinity Capture-MS Homo sapiens
17 APOE 348
Two-hybrid Homo sapiens
18 HSP90AB1 3326
Cross-Linking-MS (XL-MS) Homo sapiens
19 ARMC8 25852
Affinity Capture-MS Homo sapiens
20 PLEKHA5 54477
Two-hybrid Homo sapiens
21 CDH1 999
Proximity Label-MS Homo sapiens
22 MYC  
Affinity Capture-MS Homo sapiens
23 NTRK1 4914
Affinity Capture-MS Homo sapiens
24 EPHA2 1969
Proximity Label-MS Homo sapiens
25 CTNNA1 1495
Proximity Label-MS Homo sapiens
26 LCK 3932
Proximity Label-MS Homo sapiens
View the network image/svg+xml



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