Gene description for CAMK2A
Gene name calcium/calmodulin-dependent protein kinase II alpha
Gene symbol CAMK2A
Other names/aliases CAMKA
Species Homo sapiens
 Database cross references - CAMK2A
ExoCarta ExoCarta_815
Vesiclepedia VP_815
Entrez Gene 815
HGNC 1460
MIM 114078
UniProt Q9UQM7  
 CAMK2A identified in exosomes derived from the following tissue/cell type
Retinal pigment epithelial cells 35333565    
Retinal pigment epithelial cells 35333565    
 Gene ontology annotations for CAMK2A
Molecular Function
    protein serine/threonine kinase activity GO:0004674 IDA
    protein serine/threonine kinase activity GO:0004674 ISS
    calcium/calmodulin-dependent protein kinase activity GO:0004683 IBA
    calcium/calmodulin-dependent protein kinase activity GO:0004683 IDA
    calcium/calmodulin-dependent protein kinase activity GO:0004683 TAS
    protein binding GO:0005515 IPI
    calmodulin binding GO:0005516 IBA
    calmodulin binding GO:0005516 IPI
    ATP binding GO:0005524 IEA
    kinase activity GO:0016301 IDA
    glutamate receptor binding GO:0035254 ISS
    identical protein binding GO:0042802 IPI
    protein homodimerization activity GO:0042803 IPI
    metal ion binding GO:0046872 IEA
    protein serine kinase activity GO:0106310 IEA
Biological Process
    G1/S transition of mitotic cell cycle GO:0000082 ISS
    response to ischemia GO:0002931 ISS
    protein phosphorylation GO:0006468 IDA
    calcium ion transport GO:0006816 ISS
    positive regulation of cardiac muscle cell apoptotic process GO:0010666 ISS
    peptidyl-serine phosphorylation GO:0018105 ISS
    cellular response to interferon-beta GO:0035458 IDA
    angiotensin-activated signaling pathway GO:0038166 IDA
    positive regulation of receptor signaling pathway via JAK-STAT GO:0046427 IDA
    protein autophosphorylation GO:0046777 ISS
    regulation of neurotransmitter secretion GO:0046928 ISS
    regulation of neuronal synaptic plasticity GO:0048168 IBA
    regulation of neuronal synaptic plasticity GO:0048168 ISS
    positive regulation of NF-kappaB transcription factor activity GO:0051092 IMP
    negative regulation of hydrolase activity GO:0051346 ISS
    positive regulation of calcium ion transport GO:0051928 ISS
    dendritic spine development GO:0060996 IMP
    cellular response to type II interferon GO:0071346 IDA
    regulation of mitochondrial membrane permeability involved in apoptotic process GO:1902108 ISS
    regulation of protein localization to plasma membrane GO:1903076 IBA
    peptidyl-threonine autophosphorylation GO:1990443 IMP
    regulation of endocannabinoid signaling pathway GO:2000124 ISS
    regulation of neuron migration GO:2001222 IMP
Subcellular Localization
    nucleus GO:0005634 HDA
    nucleoplasm GO:0005654 TAS
    cytoplasm GO:0005737 IBA
    mitochondrion GO:0005739 ISS
    cytosol GO:0005829 TAS
    calcium- and calmodulin-dependent protein kinase complex GO:0005954 IDA
    postsynaptic density GO:0014069 IBA
    endocytic vesicle membrane GO:0030666 TAS
    neuron projection GO:0043005 IBA
    dendritic spine GO:0043197 IDA
 Experiment description of studies that identified CAMK2A in exosomes
1
Experiment ID 834
MISEV standards
✔
EM
Biophysical techniques
✔
LAMP2|CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P"
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 835
MISEV standards
✔
EM
Biophysical techniques
✔
CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P"
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for CAMK2A
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 EIF4G3 8672
Affinity Capture-MS Homo sapiens
2 GPM6A 2823
Affinity Capture-MS Homo sapiens
3 ITGA2B 3674
Biochemical Activity Homo sapiens
4 RBM14-RBM4 100526737
Affinity Capture-MS Homo sapiens
5 SMG6  
Affinity Capture-MS Homo sapiens
6 Rad23a  
Affinity Capture-Western Rattus norvegicus
7 ANGEL2 90806
Affinity Capture-MS Homo sapiens
8 WDR76  
Affinity Capture-MS Homo sapiens
9 TAB1 10454
Affinity Capture-MS Homo sapiens
10 NIT1 4817
Affinity Capture-MS Homo sapiens
11 TRIM55  
Two-hybrid Homo sapiens
12 RPUSD4 84881
Affinity Capture-MS Homo sapiens
13 KANSL3  
Affinity Capture-MS Homo sapiens
14 MOK  
Affinity Capture-MS Homo sapiens
15 DTL  
Affinity Capture-MS Homo sapiens
16 HNRNPUL2 221092
Affinity Capture-MS Homo sapiens
17 eag  
Reconstituted Complex Drosophila melanogaster
18 ARL3 403
Affinity Capture-MS Homo sapiens
19 Lrrc7  
Two-hybrid Rattus norvegicus
Far Western Rattus norvegicus
Affinity Capture-Western Rattus norvegicus
Two-hybrid Rattus norvegicus
20 C11orf30  
Affinity Capture-MS Homo sapiens
21 ARHGAP32  
Affinity Capture-MS Homo sapiens
22 GNAO1 2775
Co-fractionation Homo sapiens
23 CHMP5 51510
Affinity Capture-MS Homo sapiens
24 GABBR1  
Affinity Capture-Western Homo sapiens
25 NUFIP1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
26 DUSP11  
Affinity Capture-MS Homo sapiens
27 CALM3 808
Affinity Capture-MS Homo sapiens
28 CREB1  
Biochemical Activity Homo sapiens
29 PUSL1  
Affinity Capture-MS Homo sapiens
30 PLCG1 5335
Affinity Capture-Western Homo sapiens
31 PLD2 5338
Affinity Capture-MS Homo sapiens
32 CAMK2B 816
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
33 LACTB 114294
Affinity Capture-MS Homo sapiens
34 Arc  
Affinity Capture-Western Mus musculus
35 SENP5  
Affinity Capture-MS Homo sapiens
36 CDCA8 55143
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
37 GTSE1 51512
Affinity Capture-MS Homo sapiens
38 Psmc5 81827
Affinity Capture-Western Rattus norvegicus
39 MYCN  
Affinity Capture-MS Homo sapiens
40 RRP7A 27341
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
41 RAD23A 5886
Affinity Capture-Western Homo sapiens
42 PRNP 5621
Affinity Capture-MS Homo sapiens
43 AGBL5  
Affinity Capture-MS Homo sapiens
44 YWHAB 7529
Biochemical Activity Homo sapiens
45 CAMK2A 815
Biochemical Activity Homo sapiens
Co-localization Homo sapiens
Biochemical Activity Homo sapiens
Biochemical Activity Homo sapiens
Biochemical Activity Homo sapiens
Co-localization Homo sapiens
Biochemical Activity Homo sapiens
Biochemical Activity Homo sapiens
46 GRIA1  
Biochemical Activity Homo sapiens
47 DBNL 28988
Affinity Capture-Western Homo sapiens
48 ARHGEF39 84904
Affinity Capture-MS Homo sapiens
49 PARK2  
Affinity Capture-Western Homo sapiens
50 PNKP 11284
Affinity Capture-MS Homo sapiens
51 RAB21 23011
Affinity Capture-MS Homo sapiens
52 XRN1 54464
Affinity Capture-MS Homo sapiens
53 ZMYM1 79830
Affinity Capture-MS Homo sapiens
54 MAPT  
Biochemical Activity Homo sapiens
55 GIT1 28964
Affinity Capture-Western Homo sapiens
56 NUDC 10726
Cross-Linking-MS (XL-MS) Homo sapiens
57 ACTN4 81
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
58 TAB2  
Affinity Capture-MS Homo sapiens
59 DCTN1 1639
Affinity Capture-MS Homo sapiens
60 CDK5R1  
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
61 ARMC1 55156
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
62 SMAD2 4087
Co-localization Homo sapiens
63 YWHAQ 10971
Affinity Capture-Western Homo sapiens
64 EIF4G2 1982
Affinity Capture-MS Homo sapiens
65 GRIN2A  
Reconstituted Complex Homo sapiens
Biochemical Activity Homo sapiens
66 LYPLA1 10434
Affinity Capture-MS Homo sapiens
67 HDAC5 10014
Affinity Capture-Western Homo sapiens
68 THAP11 57215
Affinity Capture-MS Homo sapiens
69 CYLD  
Affinity Capture-Western Homo sapiens
70 TRIM63  
Two-hybrid Homo sapiens
71 KCNC1  
Affinity Capture-Western Homo sapiens
72 ACTN1 87
Co-localization Homo sapiens
73 DNAAF2  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
74 FNDC3B 64778
Affinity Capture-MS Homo sapiens
75 SMCR8 140775
Affinity Capture-MS Homo sapiens
76 MAP7 9053
Affinity Capture-MS Homo sapiens
77 SMCHD1 23347
Affinity Capture-MS Homo sapiens
78 GRM5  
Reconstituted Complex Homo sapiens
79 NOS3  
Affinity Capture-Western Homo sapiens
80 DLG1 1739
Affinity Capture-Western Homo sapiens
Biochemical Activity Homo sapiens
81 ELP3 55140
Affinity Capture-MS Homo sapiens
82 FKBP8 23770
Affinity Capture-Western Homo sapiens
83 BAP1 8314
Affinity Capture-MS Homo sapiens
84 MAP3K7  
Affinity Capture-MS Homo sapiens
85 ELP2 55250
Affinity Capture-MS Homo sapiens
86 KIAA0895  
Affinity Capture-MS Homo sapiens
87 PLEKHA5 54477
Affinity Capture-MS Homo sapiens
88 PATL1 219988
Affinity Capture-MS Homo sapiens
89 MTG1  
Affinity Capture-MS Homo sapiens
90 GRIN2B  
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
Biochemical Activity Homo sapiens
91 PKP4 8502
Affinity Capture-MS Homo sapiens
92 PSMC5 5705
Biochemical Activity Homo sapiens
93 CACNA1B 774
Reconstituted Complex Homo sapiens
94 SLC6A3  
Affinity Capture-Western Homo sapiens
95 HAX1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
96 AKT1 207
Affinity Capture-MS Homo sapiens
97 PMFBP1 83449
Affinity Capture-MS Homo sapiens
98 Ostm1  
Affinity Capture-MS Mus musculus
99 CAMK2G 818
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
100 RBMS2 5939
Affinity Capture-MS Homo sapiens
101 FAM120A 23196
Affinity Capture-MS Homo sapiens
102 PIP5K1A 8394
Affinity Capture-MS Homo sapiens
103 IKBKAP 8518
Affinity Capture-MS Homo sapiens
104 SHANK3 85358
Affinity Capture-Western Homo sapiens
105 RIMS1  
Biochemical Activity Homo sapiens
106 DLG3 1741
Affinity Capture-MS Homo sapiens
107 NINL  
Affinity Capture-MS Homo sapiens
108 SECISBP2L  
Affinity Capture-MS Homo sapiens
109 PTTG1  
Biochemical Activity Homo sapiens
110 DCTN2 10540
Affinity Capture-MS Homo sapiens
111 SSBP2  
Affinity Capture-MS Homo sapiens
112 RCHY1  
Biochemical Activity Homo sapiens
113 HSP90AA1 3320
Affinity Capture-Luminescence Homo sapiens
114 SNRK  
Affinity Capture-MS Homo sapiens
115 ATF1  
Biochemical Activity Homo sapiens
116 HNRNPK 3190
Co-fractionation Homo sapiens
117 ATP6V0C 527
Affinity Capture-MS Homo sapiens
118 INA 9118
Affinity Capture-MS Homo sapiens
119 DGUOK  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
120 LIN54  
Affinity Capture-MS Homo sapiens
121 CAMK2D 817
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
122 CUL4A 8451
Affinity Capture-MS Homo sapiens
123 DNAH14  
Affinity Capture-MS Homo sapiens
124 LRRC7 57554
Reconstituted Complex Homo sapiens
View the network image/svg+xml
 Pathways in which CAMK2A is involved
PathwayEvidenceSource
Activation of NMDA receptors and postsynaptic events IEA Reactome
Activation of NMDA receptors and postsynaptic events TAS Reactome
Assembly and cell surface presentation of NMDA receptors IEA Reactome
Beta-catenin independent WNT signaling TAS Reactome
Ca-dependent events IEA Reactome
Ca2+ pathway TAS Reactome
Calmodulin induced events IEA Reactome
CaM pathway IEA Reactome
CaMK IV-mediated phosphorylation of CREB IEA Reactome
Cardiac conduction TAS Reactome
Cellular response to heat stress TAS Reactome
Cellular responses to stimuli TAS Reactome
Cellular responses to stress TAS Reactome
CREB1 phosphorylation through NMDA receptor-mediated activation of RAS signaling IEA Reactome
Cytokine Signaling in Immune system TAS Reactome
DAG and IP3 signaling IEA Reactome
Disease TAS Reactome
Diseases of signal transduction by growth factor receptors and second messengers TAS Reactome
G alpha (i) signalling events IEA Reactome
G-protein mediated events IEA Reactome
Gene expression (Transcription) IEA Reactome
Generic Transcription Pathway IEA Reactome
Glutamate binding, activation of AMPA receptors and synaptic plasticity TAS Reactome
GPCR downstream signalling IEA Reactome
HSF1-dependent transactivation TAS Reactome
Immune System TAS Reactome
Interferon gamma signaling TAS Reactome
Interferon Signaling TAS Reactome
Intracellular signaling by second messengers IEA Reactome
Ion channel transport TAS Reactome
Ion homeostasis TAS Reactome
Ion transport by P-type ATPases TAS Reactome
Long-term potentiation IEA Reactome
MAPK family signaling cascades TAS Reactome
MAPK1/MAPK3 signaling TAS Reactome
Muscle contraction TAS Reactome
Negative regulation of NMDA receptor-mediated neuronal transmission IEA Reactome
Neuronal System IEA Reactome
Neuronal System TAS Reactome
Neurotransmitter receptors and postsynaptic signal transmission IEA Reactome
Neurotransmitter receptors and postsynaptic signal transmission TAS Reactome
Oncogenic MAPK signaling TAS Reactome
Opioid Signalling IEA Reactome
Paradoxical activation of RAF signaling by kinase inactive BRAF TAS Reactome
Phase 0 - rapid depolarisation TAS Reactome
PLC beta mediated events IEA Reactome
Post NMDA receptor activation events IEA Reactome
RAF activation TAS Reactome
RAF/MAP kinase cascade TAS Reactome
Ras activation upon Ca2+ influx through NMDA receptor IEA Reactome
Regulation of MECP2 expression and activity IEA Reactome
RNA Polymerase II Transcription IEA Reactome
Signal Transduction TAS Reactome
Signal Transduction IEA Reactome
Signaling by BRAF and RAF1 fusions TAS Reactome
Signaling by GPCR IEA Reactome
Signaling by moderate kinase activity BRAF mutants TAS Reactome
Signaling by RAF1 mutants TAS Reactome
Signaling by RAS mutants TAS Reactome
Signaling by WNT TAS Reactome
Signaling downstream of RAS mutants TAS Reactome
Trafficking of AMPA receptors TAS Reactome
Transcriptional Regulation by MECP2 IEA Reactome
Transmission across Chemical Synapses IEA Reactome
Transmission across Chemical Synapses TAS Reactome
Transport of small molecules TAS Reactome
Unblocking of NMDA receptors, glutamate binding and activation TAS Reactome
Unblocking of NMDA receptors, glutamate binding and activation IEA Reactome





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