Gene description for DLG1
Gene name discs, large homolog 1 (Drosophila)
Gene symbol DLG1
Other names/aliases DLGH1
SAP-97
SAP97
dJ1061C18.1.1
hdlg
Species Homo sapiens
 Database cross references - DLG1
ExoCarta ExoCarta_1739
Vesiclepedia VP_1739
Entrez Gene 1739
HGNC 2900
MIM 601014
UniProt Q12959  
 DLG1 identified in sEVs derived from the following tissue/cell type
B cells 20458337    
B cells 20458337    
B cells 20458337    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Colorectal cancer cells 23161513    
Colorectal cancer cells 23161513    
Colorectal cancer cells 23161513    
Colorectal cancer cells 34887515    
Gastric cancer cells 33991177    
Gastric cancer cells 33991177    
Gastric cancer cells 33991177    
Hepatocellular carcinoma cells 26054723    
Melanoma cells 25950383    
Melanoma cells 25950383    
Melanoma cells 25950383    
Mesenchymal stem cells 36408942    
Neuroblastoma cells 25944692    
Ovarian cancer cells 23333927    
Ovarian cancer cells 23333927    
Prostate cancer cells 25844599    
Retinal pigment epithelial cells 35333565    
Retinal pigment epithelial cells 35333565    
Thymus 23844026    
 Gene ontology annotations for DLG1
Molecular Function
    guanylate kinase activity GO:0004385 TAS
    phosphoprotein phosphatase activity GO:0004721 TAS
    protein binding GO:0005515 IPI
    cytoskeletal protein binding GO:0008092 TAS
    potassium channel regulator activity GO:0015459 IDA
    potassium channel regulator activity GO:0015459 ISS
    potassium channel regulator activity GO:0015459 NAS
    kinase binding GO:0019900 IDA
    protein kinase binding GO:0019901 IBA
    phosphatase binding GO:0019902 IPI
    ionotropic glutamate receptor binding GO:0035255 IBA
    transmembrane transporter binding GO:0044325 IPI
    cadherin binding GO:0045296 HDA
    molecular adaptor activity GO:0060090 IEA
    L27 domain binding GO:0097016 IPI
    structural constituent of postsynaptic density GO:0098919 IEA
Biological Process
    negative regulation of transcription by RNA polymerase II GO:0000122 IMP
    branching involved in ureteric bud morphogenesis GO:0001658 IEA
    immunological synapse formation GO:0001771 IEA
    endothelial cell proliferation GO:0001935 IDA
    lens development in camera-type eye GO:0002088 IEA
    actin filament organization GO:0007015 IDA
    establishment or maintenance of cell polarity GO:0007163 TAS
    chemical synaptic transmission GO:0007268 IBA
    nervous system development GO:0007399 IBA
    positive regulation of cell population proliferation GO:0008284 IEA
    regulation of cell shape GO:0008360 IMP
    actin filament polymerization GO:0030041 IEA
    peristalsis GO:0030432 IEA
    positive regulation of actin filament polymerization GO:0030838 IEA
    cortical actin cytoskeleton organization GO:0030866 IDA
    astral microtubule organization GO:0030953 IMP
    protein-containing complex localization GO:0031503 IMP
    membrane raft organization GO:0031579 IEA
    regulation of myelination GO:0031641 IEA
    protein localization to synapse GO:0035418 IBA
    T cell proliferation GO:0042098 IEA
    negative regulation of T cell proliferation GO:0042130 IEA
    regulation of membrane potential GO:0042391 IDA
    amyloid precursor protein metabolic process GO:0042982 IEA
    receptor clustering GO:0043113 IBA
    positive regulation of potassium ion transport GO:0043268 IDA
    phosphatidylinositol 3-kinase/protein kinase B signal transduction GO:0043491 IEA
    cortical microtubule organization GO:0043622 IMP
    establishment or maintenance of epithelial cell apical/basal polarity GO:0045197 IBA
    establishment or maintenance of epithelial cell apical/basal polarity GO:0045197 NAS
    GMP metabolic process GO:0046037 IEA
    GDP metabolic process GO:0046710 IEA
    reproductive structure development GO:0048608 IEA
    embryonic skeletal system morphogenesis GO:0048704 IEA
    smooth muscle tissue development GO:0048745 IEA
    negative regulation of epithelial cell proliferation GO:0050680 IEA
    establishment of centrosome localization GO:0051660 IMP
    negative regulation of phosphatidylinositol 3-kinase/protein kinase B signal transduction GO:0051898 IEA
    hard palate development GO:0060022 IEA
    negative regulation of ERK1 and ERK2 cascade GO:0070373 IMP
    bicellular tight junction assembly GO:0070830 IDA
    protein localization to plasma membrane GO:0072659 IMP
    protein localization to plasma membrane GO:0072659 TAS
    receptor localization to synapse GO:0097120 IBA
    cell-cell adhesion GO:0098609 IBA
    cell-cell adhesion GO:0098609 IDA
    regulation of ventricular cardiac muscle cell action potential GO:0098911 ISS
    membrane repolarization during ventricular cardiac muscle cell action potential GO:0098915 ISS
    regulation of postsynaptic membrane neurotransmitter receptor levels GO:0099072 IBA
    maintenance of postsynaptic density structure GO:0099562 IEA
    neurotransmitter receptor localization to postsynaptic specialization membrane GO:0099645 IEA
    regulation of non-canonical NF-kappaB signal transduction GO:1901222 IMP
    regulation of sodium ion transmembrane transport GO:1902305 TAS
    regulation of protein localization to synapse GO:1902473 IDA
    positive regulation of protein localization to plasma membrane GO:1903078 IDA
    regulation of potassium ion import GO:1903286 ISS
    negative regulation of p38MAPK cascade GO:1903753 IMP
    regulation of potassium ion export across plasma membrane GO:1903764 ISS
    negative regulation of G1/S transition of mitotic cell cycle GO:2000134 IMP
Subcellular Localization
    immunological synapse GO:0001772 IDA
    immunological synapse GO:0001772 TAS
    basement membrane GO:0005604 IEA
    nucleus GO:0005634 IDA
    cytoplasm GO:0005737 IDA
    endoplasmic reticulum GO:0005783 IDA
    endoplasmic reticulum membrane GO:0005789 IEA
    Golgi apparatus GO:0005794 IDA
    cytosol GO:0005829 TAS
    microtubule GO:0005874 IDA
    plasma membrane GO:0005886 IDA
    plasma membrane GO:0005886 TAS
    cell-cell junction GO:0005911 IDA
    adherens junction GO:0005912 NAS
    bicellular tight junction GO:0005923 IDA
    cytoplasmic side of plasma membrane GO:0009898 IDA
    intercalated disc GO:0014704 TAS
    basolateral plasma membrane GO:0016323 IBA
    basolateral plasma membrane GO:0016323 IDA
    apical plasma membrane GO:0016324 IEA
    lateral plasma membrane GO:0016328 IEA
    cell junction GO:0030054 IDA
    cell projection membrane GO:0031253 IEA
    neuromuscular junction GO:0031594 IBA
    node of Ranvier GO:0033268 IEA
    myelin sheath abaxonal region GO:0035748 IEA
    sarcolemma GO:0042383 IEA
    neuron projection GO:0043005 IBA
    lateral loop GO:0043219 IEA
    membrane raft GO:0045121 IEA
    perinuclear region of cytoplasm GO:0048471 IDA
    extracellular exosome GO:0070062 HDA
    MPP7-DLG1-LIN7 complex GO:0097025 IDA
    synaptic membrane GO:0097060 IDA
    postsynaptic density membrane GO:0098839 IBA
    glutamatergic synapse GO:0098978 IEA
 Experiment description of studies that identified DLG1 in sEVs
1
Experiment ID 79
MISEV standards
✘ Biophysical techniques
✔
CD81|MHCII
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 20458337    
Organism Homo sapiens
Experiment description MHC class II-associated proteins in B-cell exosomes and potential functional implications for exosome biogenesis - Sample 1
Authors "Buschow SI, van Balkom BW, Aalberts M, Heck AJ, Wauben M, Stoorvogel W."
Journal name ICB
Publication year 2010
Sample B cells
Sample name RN (HLA-DR15)
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Immunobeads (MHC Class II)
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [FT-ICR]
Western blotting
2
Experiment ID 80
MISEV standards
✘ Biophysical techniques
✔
CD81|MHCII
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 20458337    
Organism Homo sapiens
Experiment description MHC class II-associated proteins in B-cell exosomes and potential functional implications for exosome biogenesis -Sample 2
Authors "Buschow SI, van Balkom BW, Aalberts M, Heck AJ, Wauben M, Stoorvogel W."
Journal name ICB
Publication year 2010
Sample B cells
Sample name RN (HLA-DR15)
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Immunobeads (MHC Class II)
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [FT-ICR]
Western blotting
3
Experiment ID 81
MISEV standards
✘ Biophysical techniques
✔
CD81|MHCII
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 20458337    
Organism Homo sapiens
Experiment description MHC class II-associated proteins in B-cell exosomes and potential functional implications for exosome biogenesis - Sample 3
Authors "Buschow SI, van Balkom BW, Aalberts M, Heck AJ, Wauben M, Stoorvogel W."
Journal name ICB
Publication year 2010
Sample B cells
Sample name RN (HLA-DR15)
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Immunobeads (MHC Class II)
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [FT-ICR]
Western blotting
4
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
6
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
7
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
8
Experiment ID 207
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|HSP70|FLOT1
Enriched markers
✔
VDAC
Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23161513    
Organism Homo sapiens
Experiment description Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS.
Authors "Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ."
Journal name Mol Cell Proteomics
Publication year 2012
Sample Colorectal cancer cells
Sample name DKO-1
Isolation/purification methods Differential centrifugation
Filtration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
9
Experiment ID 208
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|HSP70|FLOT1
Enriched markers
✔
VDAC
Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23161513    
Organism Homo sapiens
Experiment description Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS.
Authors "Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ."
Journal name Mol Cell Proteomics
Publication year 2012
Sample Colorectal cancer cells
Sample name Dks-8
Isolation/purification methods Differential centrifugation
Filtration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
10
Experiment ID 209
MISEV standards
✘ Biophysical techniques
✔
TSG101|HSP70|FLOT1
Enriched markers
✔
VDAC
Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23161513    
Organism Homo sapiens
Experiment description Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS.
Authors "Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ."
Journal name Mol Cell Proteomics
Publication year 2012
Sample Colorectal cancer cells
Sample name DLD-1
Isolation/purification methods Differential centrifugation
Filtration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
11
Experiment ID 1203
MISEV standards
✔
EM
Biophysical techniques
✔
SDCBP|FLOT1|CD9|CD81|CD63|EPCAM|GAPDH|LAMP1|TFRC|CD151|CD82|LAMP2|RAB35|TSG101|FLOT2|RAB5B|ICAM1|RAB5A
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 34887515    
Organism Homo sapiens
Experiment description Supermeres are functional extracellular nanoparticles replete with disease biomarkers and therapeutic targets
Authors "Zhang Q, Jeppesen DK, Higginbotham JN, Graves-Deal R, Trinh VQ, Ramirez MA, Sohn Y, Neininger AC, Taneja N, McKinley ET, Niitsu H, Cao Z, Evans R, Glass SE, Ray KC, Fissell WH, Hill S, Rose KL, Huh WJ, Washington MK, Ayers GD, Burnette DT, Sharma S, Rome LH, Franklin JL, Lee YA, Liu Q, Coffey RJ."
Journal name Nat Cell Biol
Publication year 2021
Sample Colorectal cancer cells
Sample name DiFi
Isolation/purification methods Differential centrifugation
Filtration
Centrifugal ultrafiltration
Ultracentrifugation
OptiPrep density gradient centrifugation
Flotation density -
Molecules identified in the study Protein
miRNA
Methods used in the study Western blotting
Mass spectrometry
RNA sequencing
12
Experiment ID 363
MISEV standards
✘ Biophysical techniques
✔
TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1
Enriched markers
✔
DCLK1
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 33991177    
Organism Homo sapiens
Experiment description Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells
Authors "Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M."
Journal name Proteomics
Publication year 2021
Sample Gastric cancer cells
Sample name MKN1 - 100K pellet
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
13
Experiment ID 364
MISEV standards
✘ Biophysical techniques
✔
TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1
Enriched markers
✔
DCLK1
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 33991177    
Organism Homo sapiens
Experiment description Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells
Authors "Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M."
Journal name Proteomics
Publication year 2021
Sample Gastric cancer cells
Sample name MKN1 - 100K pellet
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
14
Experiment ID 365
MISEV standards
✘ Biophysical techniques
✔
TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1
Enriched markers
✔
DCLK1
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 33991177    
Organism Homo sapiens
Experiment description Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells
Authors "Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M."
Journal name Proteomics
Publication year 2021
Sample Gastric cancer cells
Sample name MKN1 - 100K pellet
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
15
Experiment ID 235
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|Alix|HSC70|GAPDH
Enriched markers
✔
HSP90B1
Negative markers
✔
qNano
Particle analysis
Identified molecule mRNA
Identification method RNA Sequencing
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors "He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N"
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocellular carcinoma cells
Sample name HKCI-8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RT-PCR
RNA Sequencing
16
Experiment ID 257
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|FLOT1
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25950383    
Organism Homo sapiens
Experiment description Proteome characterization of melanoma exosomes reveals a specific signature for metastatic cell lines
Authors "Lazar I, Clement E, Ducoux-Petit M, Denat L, Soldan V, Dauvillier S, Balor S4, Burlet-Schiltz O1, Larue L, Muller C Nieto L"
Journal name Pigment Cell Melanoma Res
Publication year 2015
Sample Melanoma cells
Sample name Daju
Isolation/purification methods Differential centrifugation
Unltracentrifugation
Sucrose density gradient
Flotation density 1.13 - 1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
17
Experiment ID 258
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|FLOT1|CD81
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25950383    
Organism Homo sapiens
Experiment description Proteome characterization of melanoma exosomes reveals a specific signature for metastatic cell lines
Authors "Lazar I, Clement E, Ducoux-Petit M, Denat L, Soldan V, Dauvillier S, Balor S4, Burlet-Schiltz O1, Larue L, Muller C Nieto L"
Journal name Pigment Cell Melanoma Res
Publication year 2015
Sample Melanoma cells
Sample name SKMEL28
Isolation/purification methods Differential centrifugation
Unltracentrifugation
Sucrose density gradient
Flotation density 1.13 - 1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
18
Experiment ID 260
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|FLOT1|CD81
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25950383    
Organism Homo sapiens
Experiment description Proteome characterization of melanoma exosomes reveals a specific signature for metastatic cell lines
Authors "Lazar I, Clement E, Ducoux-Petit M, Denat L, Soldan V, Dauvillier S, Balor S4, Burlet-Schiltz O1, Larue L, Muller C Nieto L"
Journal name Pigment Cell Melanoma Res
Publication year 2015
Sample Melanoma cells
Sample name 1205Lu
Isolation/purification methods Differential centrifugation
Unltracentrifugation
Sucrose density gradient
Flotation density 1.13 - 1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
19
Experiment ID 488
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD81|CD63|GAPDH|SDCBP|LAMP1|TFRC|UCHL1|FLOT2|LAMP2|FLOT1|ICAM1|RAB5B|CD151|RAB35|TSG101|RAB5A|CD82
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Homo sapiens
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Mesenchymal stem cells
Sample name UCMSC
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
20
Experiment ID 224
MISEV standards
✔
EM|AFM
Biophysical techniques
✔
Alix|TSG101|CD63|CD81
Enriched markers
✔
GOLGA2
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25944692    
Organism Homo sapiens
Experiment description Proteogenomic analysis reveals exosomes are more oncogenic than ectosomes
Authors "Keerthikumar S, Gangoda L, Liem M, Fonseka P, Atukorala I, Ozcitti C, Mechler A, Adda CG, Ang CS, Mathivanan S"
Journal name Oncotarget
Publication year 2015
Sample Neuroblastoma cells
Sample name SH-SY5Y
Isolation/purification methods Differential centrifugation
Ultracentrifugation
OptiPrep density gradient
Flotation density 1.10 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
Western blotting
21
Experiment ID 211
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|Alix|EpCAM|TFRC
Enriched markers
✔
cytochrome c|GOLGA2
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors "Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K."
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name IGROV1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
22
Experiment ID 212
MISEV standards
✔
CEM
Biophysical techniques
✔
TSG101|Alix|EpCAM|TFRC
Enriched markers
✔
Cytochrome C|GOLGA2
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors "Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K."
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name OVCAR-3
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
23
Experiment ID 275
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|Alix|RAB5A|CD9|CD82|CD63|CD81
Enriched markers
✔
AIF
Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25844599    
Organism Homo sapiens
Experiment description Molecular profiling of prostate cancer derived exosomes may reveal a predictive signature for response to docetaxel.
Authors "Kharaziha P, Chioureas D, Rutishauser D, Baltatzis G, Lennartsson L, Fonseca P, Azimi A, Hultenby K, Zubarev R, Ullen A, Yachnin J, Nilsson S, Panaretakis T."
Journal name Oncotarget
Publication year 2015
Sample Prostate cancer cells
Sample name DU145 - Docetaxel sensitive
Isolation/purification methods Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.12-1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry/Flow cytometry/Western blotting
24
Experiment ID 834
MISEV standards
✔
EM
Biophysical techniques
✔
LAMP2|CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P"
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
25
Experiment ID 835
MISEV standards
✔
EM
Biophysical techniques
✔
CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P"
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
26
Experiment ID 217
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|CD81|CD9|CD63
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23844026    
Organism Homo sapiens
Experiment description Characterization of human thymic exosomes.
Authors "Skogberg G, Gudmundsdottir J, van der Post S, Sandstrom K, Bruhn S, Benson M, Mincheva-Nilsson L, Baranov V, Telemo E, Ekwall O."
Journal name PLoS One
Publication year 2013
Sample Thymus
Sample name Normal-Thymus
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for DLG1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 NET1 10276
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
2 GJD3  
Proximity Label-MS Homo sapiens
3 OCLN 100506658
Proximity Label-MS Homo sapiens
4 LIN7B  
Affinity Capture-MS Homo sapiens
5 DLGAP2  
Affinity Capture-MS Homo sapiens
6 MARCKS 4082
Proximity Label-MS Homo sapiens
7 LAMP3  
Proximity Label-MS Homo sapiens
8 GJA1 2697
Proximity Label-MS Homo sapiens
9 ARHGEF26 26084
Affinity Capture-MS Homo sapiens
10 PARD3 56288
Proximity Label-MS Homo sapiens
11 GLB1 2720
Co-fractionation Homo sapiens
12 MARCH2  
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
13 UBE3A 7337
Reconstituted Complex Homo sapiens
14 LAMP2 3920
Proximity Label-MS Homo sapiens
15 STX4 6810
Proximity Label-MS Homo sapiens
16 KIF13B 23303
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
17 B3GAT1  
Proximity Label-MS Homo sapiens
18 MLLT4 4301
Proximity Label-MS Homo sapiens
19 TFAM 7019
Cross-Linking-MS (XL-MS) Homo sapiens
Cross-Linking-MS (XL-MS) Homo sapiens
20 LRRC1 55227
Two-hybrid Homo sapiens
21 KCNJ4  
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
22 KHDRBS1 10657
Protein-peptide Homo sapiens
23 GPRC5B 51704
Affinity Capture-MS Homo sapiens
24 MPP2 4355
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
25 SLC31A1 1317
Affinity Capture-MS Homo sapiens
26 KCNJ2 3759
Reconstituted Complex Homo sapiens
27 STX6 10228
Proximity Label-MS Homo sapiens
28 RAB35 11021
Proximity Label-MS Homo sapiens
29 BTRC 8945
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
30 GRIK2  
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
31 EBAG9 9166
Proximity Label-MS Homo sapiens
32 FAM20C 56975
Affinity Capture-MS Homo sapiens
33 KCNA4  
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
34 KIF14 9928
Affinity Capture-MS Homo sapiens
35 PRNP 5621
Affinity Capture-MS Homo sapiens
36 CASK 8573
Affinity Capture-Western Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Two-hybrid Homo sapiens
FRET Homo sapiens
Affinity Capture-Western Homo sapiens
37 DLGAP1  
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Far Western Homo sapiens
38 CAMK2A 815
Affinity Capture-Western Homo sapiens
Biochemical Activity Homo sapiens
39 GRIA1  
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
FRET Homo sapiens
Affinity Capture-Western Homo sapiens
40 SRP9 6726
Affinity Capture-MS Homo sapiens
41 GUCY1A2  
Affinity Capture-Western Homo sapiens
42 PRDM1  
Affinity Capture-MS Homo sapiens
43 MAPK12  
Co-localization Homo sapiens
44 PARK2  
Co-localization Homo sapiens
45 CXADR 1525
Proximity Label-MS Homo sapiens
46 DLGAP4 22839
Affinity Capture-MS Homo sapiens
47 LYN 4067
Proximity Label-MS Homo sapiens
48 MCAM 4162
Proximity Label-MS Homo sapiens
49 CERS2 29956
Affinity Capture-MS Homo sapiens
50 GJB7  
Affinity Capture-MS Homo sapiens
51 DNAJC5 80331
Proximity Label-MS Homo sapiens
52 MKRN2 23609
Affinity Capture-RNA Homo sapiens
53 DIRAS3  
Proximity Label-MS Homo sapiens
54 NTRK1 4914
Affinity Capture-MS Homo sapiens
55 LIN7C 55327
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Cross-Linking-MS (XL-MS) Homo sapiens
56 SPATA2  
Affinity Capture-MS Homo sapiens
57 LAMP1 3916
Proximity Label-MS Homo sapiens
58 CDH1 999
Proximity Label-MS Homo sapiens
59 KCNA5  
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
60 KCNJ6  
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
61 APBA1  
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
62 ADAMTS1 9510
Affinity Capture-MS Homo sapiens
63 GRIN2A  
Affinity Capture-Western Homo sapiens
Two-hybrid Homo sapiens
Reconstituted Complex Homo sapiens
64 ATP2B4 493
Reconstituted Complex Homo sapiens
65 EFNB3 1949
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
66 KCNC1  
Affinity Capture-Western Homo sapiens
67 PHLPP1  
Proximity Label-MS Homo sapiens
68 Lin7c 22343
Affinity Capture-MS Mus musculus
69 RAB5A 5868
Proximity Label-MS Homo sapiens
70 ADGRA2 25960
Affinity Capture-Western Homo sapiens
Two-hybrid Homo sapiens
Reconstituted Complex Homo sapiens
71 EPB41L4A 64097
Proximity Label-MS Homo sapiens
72 CTNNB1 1499
Proximity Label-MS Homo sapiens
73 PTEN 5728
Reconstituted Complex Homo sapiens
74 ERBB4 2066
Affinity Capture-Western Homo sapiens
75 ARF6 382
Proximity Label-MS Homo sapiens
76 ARHGEF16 27237
Affinity Capture-MS Homo sapiens
77 EFCAB11  
Affinity Capture-MS Homo sapiens
78 KIF26B  
Affinity Capture-MS Homo sapiens
79 LIN7A 8825
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
80 GRIN1 2902
FRET Homo sapiens
81 PSPC1 55269
Affinity Capture-MS Homo sapiens
82 GRIN2B  
Affinity Capture-Western Homo sapiens
Two-hybrid Homo sapiens
Protein-peptide Homo sapiens
83 LMNB1 4001
Affinity Capture-MS Homo sapiens
84 RAB9A 9367
Proximity Label-MS Homo sapiens
85 EPB41 2035
Reconstituted Complex Homo sapiens
86 TJAP1 93643
Affinity Capture-Western Homo sapiens
Two-hybrid Homo sapiens
87 TGFA  
Reconstituted Complex Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
Co-localization Homo sapiens
88 ACTN2 88
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
89 AMOT 154796
Affinity Capture-MS Homo sapiens
90 RHOB 388
Proximity Label-MS Homo sapiens
91 KRAS 3845
Affinity Capture-MS Homo sapiens
Proximity Label-MS Homo sapiens
92 CTNNA1 1495
Affinity Capture-Western Homo sapiens
93 CRIPT 9419
Reconstituted Complex Homo sapiens
94 FLOT1 10211
Proximity Label-MS Homo sapiens
95 HUWE1 10075
Affinity Capture-MS Homo sapiens
96 TULP4 56995
Affinity Capture-MS Homo sapiens
97 ADAM10 102
Affinity Capture-Western Homo sapiens
98 ADAM17 6868
Two-hybrid Homo sapiens
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Co-localization Homo sapiens
99 LAMTOR1 55004
Proximity Label-MS Homo sapiens
100 Myo6  
Two-hybrid Mus musculus
Reconstituted Complex Mus musculus
101 DLG3 1741
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
102 EZR 7430
Reconstituted Complex Homo sapiens
Proximity Label-MS Homo sapiens
103 LCK 3932
Co-localization Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
104 C11orf52 91894
Proximity Label-MS Homo sapiens
105 KCNA3  
Affinity Capture-Western Homo sapiens
106 RAB2A 5862
Proximity Label-MS Homo sapiens
107 DNAJC5B  
Proximity Label-MS Homo sapiens
108 ATP2B2 491
Two-hybrid Homo sapiens
109 RAB11A 8766
Proximity Label-MS Homo sapiens
110 EPHA2 1969
Proximity Label-MS Homo sapiens
111 CACNG2  
Affinity Capture-Western Homo sapiens
112 RAB5C 5878
Proximity Label-MS Homo sapiens
113 MAP1A 4130
Affinity Capture-Western Homo sapiens
114 PTPRE 5791
Proximity Label-MS Homo sapiens
115 HSPA12A 259217
Proximity Label-MS Homo sapiens
116 IRS1 3667
Co-fractionation Homo sapiens
117 CAV1 857
Proximity Label-MS Homo sapiens
118 KCNJ12 3768
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
119 BAI1  
Protein-peptide Homo sapiens
View the network image/svg+xml
 Pathways in which DLG1 is involved
PathwayEvidenceSource
Activation of Ca-permeable Kainate Receptor TAS Reactome
Activation of kainate receptors upon glutamate binding TAS Reactome
Activation of NMDA receptors and postsynaptic events IEA Reactome
Activation of NMDA receptors and postsynaptic events TAS Reactome
Assembly and cell surface presentation of NMDA receptors IEA Reactome
Axon guidance IEA Reactome
CREB1 phosphorylation through NMDA receptor-mediated activation of RAS signaling IEA Reactome
Developmental Biology IEA Reactome
Glutamate binding, activation of AMPA receptors and synaptic plasticity TAS Reactome
Ionotropic activity of kainate receptors TAS Reactome
L1CAM interactions IEA Reactome
Long-term potentiation IEA Reactome
MAPK family signaling cascades TAS Reactome
MAPK1/MAPK3 signaling TAS Reactome
Negative regulation of NMDA receptor-mediated neuronal transmission IEA Reactome
Nervous system development IEA Reactome
Neuronal System IEA Reactome
Neuronal System TAS Reactome
Neurotransmitter receptors and postsynaptic signal transmission IEA Reactome
Neurotransmitter receptors and postsynaptic signal transmission TAS Reactome
NrCAM interactions IEA Reactome
Post NMDA receptor activation events IEA Reactome
Protein-protein interactions at synapses TAS Reactome
RAF/MAP kinase cascade TAS Reactome
Ras activation upon Ca2+ influx through NMDA receptor IEA Reactome
Signal Transduction TAS Reactome
Synaptic adhesion-like molecules TAS Reactome
Trafficking of AMPA receptors TAS Reactome
Transmission across Chemical Synapses IEA Reactome
Transmission across Chemical Synapses TAS Reactome
Unblocking of NMDA receptors, glutamate binding and activation TAS Reactome
Unblocking of NMDA receptors, glutamate binding and activation IEA Reactome





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