Gene description for KCNJ2
Gene name potassium channel, inwardly rectifying subfamily J, member 2
Gene symbol KCNJ2
Other names/aliases ATFB9
HHBIRK1
HHIRK1
IRK1
KIR2.1
LQT7
SQT3
Species Homo sapiens
 Database cross references - KCNJ2
ExoCarta ExoCarta_3759
Vesiclepedia VP_3759
Entrez Gene 3759
HGNC 6263
MIM 600681
UniProt P63252  
 KCNJ2 identified in exosomes derived from the following tissue/cell type
Hepatocellular carcinoma cells 26054723    
Hepatocytes 26054723    
Mesenchymal stem cells 36408942    
 Gene ontology annotations for KCNJ2
Molecular Function
    phosphatidylinositol-4,5-bisphosphate binding GO:0005546 IDA
    inward rectifier potassium channel activity GO:0005242 IDA
    identical protein binding GO:0042802 IEA
    voltage-gated potassium channel activity involved in cardiac muscle cell action potential repolarization GO:0086008 IMP
Biological Process
    regulation of membrane repolarization GO:0060306 IDA
    cellular response to mechanical stimulus GO:0071260 IEA
    positive regulation of potassium ion transmembrane transport GO:1901381 IEA
    magnesium ion transport GO:0015693 IEA
    cellular potassium ion homeostasis GO:0030007 TAS
    cardiac muscle cell action potential involved in contraction GO:0086002 IMP
    protein homotetramerization GO:0051289 IDA
    membrane repolarization during action potential GO:0086011 IMP
    regulation of skeletal muscle contraction via regulation of action potential GO:0014861 IMP
    relaxation of cardiac muscle GO:0055119 IMP
    membrane repolarization during cardiac muscle cell action potential GO:0086013 TAS
    membrane depolarization during cardiac muscle cell action potential GO:0086012 TAS
    synaptic transmission GO:0007268 TAS
    potassium ion import GO:0010107 IDA
    potassium ion transport GO:0006813 IDA
    relaxation of skeletal muscle GO:0090076 IMP
    potassium ion transmembrane transport GO:0071805 IDA
    regulation of heart rate by cardiac conduction GO:0086091 IMP
    regulation of resting membrane potential GO:0060075 TAS
Subcellular Localization
    smooth endoplasmic reticulum GO:0005790 IEA
    intercalated disc GO:0014704 IEA
    Golgi apparatus GO:0005794 IEA
    voltage-gated potassium channel complex GO:0008076 IDA
    neuronal cell body GO:0043025 IEA
    dendritic spine GO:0043197 IEA
    T-tubule GO:0030315 IEA
    plasma membrane GO:0005886 TAS
    integral component of plasma membrane GO:0005887 TAS
    intrinsic component of membrane GO:0031224 IDA
    rough endoplasmic reticulum GO:0005791 IEA
 Experiment description of studies that identified KCNJ2 in exosomes
1
Experiment ID 236
MISEV standards
✔
EM
EV Biophysical techniques
✔
TSG101|Alix|HSC70|GAPDH
EV Enriched markers
✔
HSP90B1
EV Negative markers
✔
qNano
EV Particle analysis
Identified molecule mRNA
Identification method RNA Sequencing
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocellular carcinoma cells
Sample name MHCC97L
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RT-PCR
RNA Sequencing
2
Experiment ID 237
MISEV standards
✔
EM
EV Biophysical techniques
✔
TSG101|Alix|HSC70|GAPDH
EV Enriched markers
✔
HSP90B1
EV Negative markers
✔
qNano
EV Particle analysis
Identified molecule mRNA
Identification method RNA Sequencing
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocytes
Sample name MIHA
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RNA Sequencing
3
Experiment ID 488
MISEV standards
✔
EM
EV Biophysical techniques
✔
CD9|CD81|CD63|GAPDH|SDCBP|LAMP1|TFRC|UCHL1|FLOT2|LAMP2|FLOT1|ICAM1|RAB5B|CD151|RAB35|TSG101|RAB5A|CD82
EV Enriched markers
✔
CANX
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Homo sapiens
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ.
Journal name Proteomics
Publication year 2023
Sample Mesenchymal stem cells
Sample name UCMSC
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
 Protein-protein interactions for KCNJ2
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 KCNJ11  
Invitro Homo sapiens
2 DLG1 1739
Invitro Homo sapiens
Invivo Homo sapiens
3 TRAK2 66008
Affinity Capture-Western Homo sapiens
Two-hybrid Homo sapiens
4 IL16  
Invivo Homo sapiens
Two-hybrid Homo sapiens
5 KCNJ8  
Invivo Homo sapiens
Invitro Homo sapiens
6 AKAP5  
Invivo Homo sapiens
Invitro Homo sapiens
7 DLG4  
Invivo Homo sapiens
Two-hybrid Homo sapiens
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