Gene description for HSPA12A
Gene name heat shock 70kDa protein 12A
Gene symbol HSPA12A
Other names/aliases -
Species Homo sapiens
 Database cross references - HSPA12A
ExoCarta ExoCarta_259217
Vesiclepedia VP_259217
Entrez Gene 259217
HGNC 19022
MIM 610701
UniProt O43301  
 HSPA12A identified in exosomes derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Mesenchymal stem cells 36408942    
Neuroblastoma cells 25944692    
Thymus 23844026    
Urine 19056867    
 Gene ontology annotations for HSPA12A
Molecular Function
    protein binding GO:0005515 IPI
    ATP binding GO:0005524 IEA
Subcellular Localization
    nucleus GO:0005634 IEA
    cytoplasm GO:0005737 IEA
    extracellular exosome GO:0070062 HDA
 Experiment description of studies that identified HSPA12A in exosomes
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 488
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD81|CD63|GAPDH|SDCBP|LAMP1|TFRC|UCHL1|FLOT2|LAMP2|FLOT1|ICAM1|RAB5B|CD151|RAB35|TSG101|RAB5A|CD82
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Homo sapiens
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Mesenchymal stem cells
Sample name UCMSC
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
6
Experiment ID 224
MISEV standards
✔
EM|AFM
Biophysical techniques
✔
Alix|TSG101|CD63|CD81
Enriched markers
✔
GOLGA2
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25944692    
Organism Homo sapiens
Experiment description Proteogenomic analysis reveals exosomes are more oncogenic than ectosomes
Authors "Keerthikumar S, Gangoda L, Liem M, Fonseka P, Atukorala I, Ozcitti C, Mechler A, Adda CG, Ang CS, Mathivanan S"
Journal name Oncotarget
Publication year 2015
Sample Neuroblastoma cells
Sample name SH-SY5Y
Isolation/purification methods Differential centrifugation
Ultracentrifugation
OptiPrep density gradient
Flotation density 1.10 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
Western blotting
7
Experiment ID 217
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|CD81|CD9|CD63
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23844026    
Organism Homo sapiens
Experiment description Characterization of human thymic exosomes.
Authors "Skogberg G, Gudmundsdottir J, van der Post S, Sandstrom K, Bruhn S, Benson M, Mincheva-Nilsson L, Baranov V, Telemo E, Ekwall O."
Journal name PLoS One
Publication year 2013
Sample Thymus
Sample name Normal-Thymus
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
8
Experiment ID 63
MISEV standards
✘ Biophysical techniques
✔
AQP2
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 19056867    
Organism Homo sapiens
Experiment description Large-scale proteomics and phosphoproteomics of urinary exosomes.
Authors "Gonzales PA, Pisitkun T, Hoffert JD, Tchapyjnikov D, Star RA, Kleta R, Wang NS, Knepper MA"
Journal name JASN
Publication year 2009
Sample Urine
Sample name Urine - Normal
Isolation/purification methods Differential centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [LTQ]
Western blotting
 Protein-protein interactions for HSPA12A
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 TGOLN2 10618
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
2 SLFN11 91607
Affinity Capture-MS Homo sapiens
3 PTPRG 5793
Proximity Label-MS Homo sapiens
4 IKBKAP 8518
Proximity Label-MS Homo sapiens
5 ARHGAP35 2909
Affinity Capture-MS Homo sapiens
6 BCOR  
Affinity Capture-MS Homo sapiens
7 TMEM51 55092
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
8 SNCB 6620
Affinity Capture-MS Homo sapiens
9 CCL22 6367
Affinity Capture-MS Homo sapiens
10 GPS1 2873
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
11 ART3  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
12 TMEM9 252839
Affinity Capture-MS Homo sapiens
13 NRCAM  
Two-hybrid Homo sapiens
14 OAS3 4940
Co-fractionation Homo sapiens
15 LAMP1 3916
Proximity Label-MS Homo sapiens
16 SNAP23 8773
Proximity Label-MS Homo sapiens
17 SORL1 6653
Two-hybrid Homo sapiens
Co-localization Homo sapiens
Reconstituted Complex Homo sapiens
18 ICAM1 3383
Affinity Capture-MS Homo sapiens
19 LAMP3  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
20 VANGL1 81839
Proximity Label-MS Homo sapiens
21 TNF  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
22 AKAP12 9590
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Proximity Label-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
23 CD58 965
Affinity Capture-MS Homo sapiens
24 BSG 682
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
25 NPTN 27020
Affinity Capture-MS Homo sapiens
26 MISP 126353
Affinity Capture-MS Homo sapiens
27 M6PR 4074
Two-hybrid Homo sapiens
28 ITPR3 3710
Affinity Capture-MS Homo sapiens
29 DLG1 1739
Proximity Label-MS Homo sapiens
30 COPS3 8533
Affinity Capture-MS Homo sapiens
31 HSPA12B 116835
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
Proximity Label-MS Homo sapiens
Affinity Capture-MS Homo sapiens
32 PRCC  
Affinity Capture-MS Homo sapiens
33 MGARP  
Affinity Capture-MS Homo sapiens
34 HRAS 3265
Reconstituted Complex Homo sapiens
35 NCOA7  
Affinity Capture-MS Homo sapiens
36 CBWD2  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
37 LAMP2 3920
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
38 TMEM263 90488
Affinity Capture-MS Homo sapiens
39 WBP1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
40 CHUK 1147
Affinity Capture-MS Homo sapiens
41 SYVN1 84447
Affinity Capture-Western Homo sapiens
42 LONP2 83752
Affinity Capture-MS Homo sapiens
43 CLNS1A 1207
Proximity Label-MS Homo sapiens
44 ELP2 55250
Proximity Label-MS Homo sapiens
45 SPTBN1 6711
Proximity Label-MS Homo sapiens
46 PLEKHA4 57664
Affinity Capture-MS Homo sapiens
47 KRT222 125113
Affinity Capture-MS Homo sapiens
48 APEX1 328
Affinity Capture-RNA Homo sapiens
49 PALLD 23022
Affinity Capture-MS Homo sapiens
50 DNAJC5 80331
Proximity Label-MS Homo sapiens
51 PLEKHG4  
Affinity Capture-MS Homo sapiens
52 EPB41L3 23136
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Proximity Label-MS Homo sapiens
Affinity Capture-MS Homo sapiens
53 SPTAN1 6709
Proximity Label-MS Homo sapiens
54 AP5M1 55745
Affinity Capture-MS Homo sapiens
55 PHACTR4 65979
Affinity Capture-MS Homo sapiens
56 MED23 9439
Co-fractionation Homo sapiens
57 NAAA  
Affinity Capture-MS Homo sapiens
58 GPR45  
Affinity Capture-MS Homo sapiens
59 COPS6 10980
Affinity Capture-MS Homo sapiens
60 FAM174A 345757
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
61 EPHA2 1969
Proximity Label-MS Homo sapiens
62 DDX3X 1654
Affinity Capture-MS Homo sapiens
63 GLMP 112770
Affinity Capture-MS Homo sapiens
64 SNW1 22938
Affinity Capture-MS Homo sapiens
65 EBAG9 9166
Proximity Label-MS Homo sapiens
66 MACROD2  
Affinity Capture-MS Homo sapiens
67 NFXL1 152518
Affinity Capture-MS Homo sapiens
68 PRKAR2A 5576
Affinity Capture-MS Homo sapiens
69 PODXL 5420
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
70 COPS7B 64708
Affinity Capture-MS Homo sapiens
71 ARHGEF10 9639
Affinity Capture-MS Homo sapiens
72 CBWD1 55871
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
73 ICAM5 7087
Affinity Capture-MS Homo sapiens
74 USP32 84669
Affinity Capture-MS Homo sapiens
75 CTDSPL 10217
Proximity Label-MS Homo sapiens
76 RB1CC1 9821
Proximity Label-MS Homo sapiens
Affinity Capture-MS Homo sapiens
77 CD80 941
Affinity Capture-MS Homo sapiens
78 MGST3 4259
Affinity Capture-MS Homo sapiens
79 SLC12A2 6558
Proximity Label-MS Homo sapiens
80 CDC5L 988
Affinity Capture-MS Homo sapiens
81 DKKL1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
82 AIFM1 9131
Affinity Capture-MS Homo sapiens
View the network image/svg+xml



Perform bioinformatics analysis of your extracellular vesicle data set using FunRich, a open access standalone tool. NEW UPDATED VERSION OF FunRich available for download (12/09/2016) from here