Gene description for HSPA12B
Gene name heat shock 70kD protein 12B
Gene symbol HSPA12B
Other names/aliases C20orf60
dJ1009E24.2
Species Homo sapiens
 Database cross references - HSPA12B
ExoCarta ExoCarta_116835
Vesiclepedia VP_116835
Entrez Gene 116835
HGNC 16193
MIM 610702
UniProt Q96MM6  
 HSPA12B identified in exosomes derived from the following tissue/cell type
Mesenchymal stem cells 36408942    
Thymus 23844026    
 Gene ontology annotations for HSPA12B
Molecular Function
    protein binding GO:0005515 IPI
    ATP binding GO:0005524 IEA
 Experiment description of studies that identified HSPA12B in exosomes
1
Experiment ID 488
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD81|CD63|GAPDH|SDCBP|LAMP1|TFRC|UCHL1|FLOT2|LAMP2|FLOT1|ICAM1|RAB5B|CD151|RAB35|TSG101|RAB5A|CD82
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Homo sapiens
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Mesenchymal stem cells
Sample name UCMSC
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 217
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|CD81|CD9|CD63
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23844026    
Organism Homo sapiens
Experiment description Characterization of human thymic exosomes.
Authors "Skogberg G, Gudmundsdottir J, van der Post S, Sandstrom K, Bruhn S, Benson M, Mincheva-Nilsson L, Baranov V, Telemo E, Ekwall O."
Journal name PLoS One
Publication year 2013
Sample Thymus
Sample name Normal-Thymus
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for HSPA12B
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 NOTCH2NL 388677
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
2 ARHGAP35 2909
Affinity Capture-MS Homo sapiens
3 VWA8 23078
Affinity Capture-MS Homo sapiens
4 PPP6R2 9701
Proximity Label-MS Homo sapiens
5 SPTAN1 6709
Proximity Label-MS Homo sapiens
6 ZC3H7A  
Proximity Label-MS Homo sapiens
Affinity Capture-MS Homo sapiens
7 TRAF2 7186
Two-hybrid Homo sapiens
8 HK1 3098
Affinity Capture-MS Homo sapiens
9 TRIM25 7706
Affinity Capture-MS Homo sapiens
10 PAGR1  
Affinity Capture-MS Homo sapiens
11 AIFM1 9131
Affinity Capture-MS Homo sapiens
12 CEP89 84902
Proximity Label-MS Homo sapiens
13 PMS1  
Proximity Label-MS Homo sapiens
14 PODXL 5420
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
15 YTHDF1 54915
Affinity Capture-MS Homo sapiens
16 AKAP12 9590
Proximity Label-MS Homo sapiens
Affinity Capture-MS Homo sapiens
17 DNAJA2 10294
Affinity Capture-MS Homo sapiens
18 BSG 682
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
19 PPM1E  
Affinity Capture-MS Homo sapiens
20 KRTAP10-8  
Two-hybrid Homo sapiens
21 FERMT2 10979
Proximity Label-MS Homo sapiens
22 KRT34  
Two-hybrid Homo sapiens
23 BUB1B  
Proximity Label-MS Homo sapiens
24 NBPF19  
Two-hybrid Homo sapiens
25 ADGRL2 23266
Proximity Label-MS Homo sapiens
26 NR3C1 2908
Proximity Label-MS Homo sapiens
27 USP10 9100
Proximity Label-MS Homo sapiens
28 MALSU1  
Affinity Capture-MS Homo sapiens
29 KRT31 3881
Two-hybrid Homo sapiens
30 AQP3  
Affinity Capture-MS Homo sapiens
31 SDCCAG3  
Affinity Capture-MS Homo sapiens
32 RCN1 5954
Affinity Capture-MS Homo sapiens
33 SPTBN1 6711
Proximity Label-MS Homo sapiens
34 MAPRE1 22919
Proximity Label-MS Homo sapiens
35 MAP7D2 256714
Proximity Label-MS Homo sapiens
36 FAM193A  
Proximity Label-MS Homo sapiens
37 WDR62  
Proximity Label-MS Homo sapiens
38 PLEKHG4  
Affinity Capture-MS Homo sapiens
39 SNAP23 8773
Proximity Label-MS Homo sapiens
40 FCGR3A  
Affinity Capture-MS Homo sapiens
41 EPB41L3 23136
Affinity Capture-MS Homo sapiens
42 CCDC77  
Proximity Label-MS Homo sapiens
43 WDR41  
Proximity Label-MS Homo sapiens
44 CYSRT1 375791
Two-hybrid Homo sapiens
45 VANGL1 81839
Proximity Label-MS Homo sapiens
46 NME7 29922
Affinity Capture-MS Homo sapiens
47 RASAL2 9462
Proximity Label-MS Homo sapiens
48 FAM174A 345757
Affinity Capture-MS Homo sapiens
49 DCTN1 1639
Proximity Label-MS Homo sapiens
50 CEP131 22994
Proximity Label-MS Homo sapiens
51 EPHA2 1969
Proximity Label-MS Homo sapiens
52 KRT40  
Two-hybrid Homo sapiens
53 RCN2 5955
Affinity Capture-MS Homo sapiens
54 TNIK 23043
Proximity Label-MS Homo sapiens
55 PPP6R1 22870
Affinity Capture-MS Homo sapiens
56 HSPA12A 259217
Affinity Capture-MS Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
Proximity Label-MS Homo sapiens
57 AP3D1 8943
Proximity Label-MS Homo sapiens
58 ARHGEF10 9639
Proximity Label-MS Homo sapiens
59 SNX29  
Proximity Label-MS Homo sapiens
60 CBWD1 55871
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
61 EEF2K  
Affinity Capture-MS Homo sapiens
Proximity Label-MS Homo sapiens
Affinity Capture-MS Homo sapiens
62 FTSJ3 117246
Proximity Label-MS Homo sapiens
63 AP5M1 55745
Affinity Capture-MS Homo sapiens
64 EFEMP2 30008
Two-hybrid Homo sapiens
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