Gene description for ARHGAP35
Gene name Rho GTPase activating protein 35
Gene symbol ARHGAP35
Other names/aliases GRF-1
GRLF1
P190-A
P190A
p190ARhoGAP
p190RhoGAP
Species Homo sapiens
 Database cross references - ARHGAP35
ExoCarta ExoCarta_2909
Vesiclepedia VP_2909
Entrez Gene 2909
HGNC 4591
MIM 605277
UniProt Q9NRY4  
 ARHGAP35 identified in sEVs derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Colorectal cancer cells 23161513    
Colorectal cancer cells 23161513    
Colorectal cancer cells 23161513    
Endothelial cells 26027894    
Mesenchymal stem cells 36408942    
Neuroblastoma cells 25944692    
 Gene ontology annotations for ARHGAP35
Molecular Function
    DNA binding GO:0003677 IEA
    GTPase activity GO:0003924 IEA
    GTPase activator activity GO:0005096 IBA
    GTPase activator activity GO:0005096 IDA
    GTPase activator activity GO:0005096 TAS
    GTP binding GO:0005525 IEA
    phospholipid binding GO:0005543 IDA
Biological Process
    neural tube closure GO:0001843 IEA
    Rho protein signal transduction GO:0007266 IBA
    axon guidance GO:0007411 ISS
    axonal fasciculation GO:0007413 ISS
    regulation of actin polymerization or depolymerization GO:0008064 ISS
    regulation of cell shape GO:0008360 IEA
    regulation of cell size GO:0008361 IBA
    positive regulation of neuron projection development GO:0010976 ISS
    cell migration GO:0016477 ISS
    central nervous system neuron axonogenesis GO:0021955 ISS
    mammary gland development GO:0030879 ISS
    forebrain development GO:0030900 IEA
    establishment or maintenance of actin cytoskeleton polarity GO:0030950 ISS
    regulation of actin cytoskeleton organization GO:0032956 ISS
    negative regulation of Rho protein signal transduction GO:0035024 ISS
    camera-type eye development GO:0043010 IEA
    negative regulation of vascular permeability GO:0043116 IEA
    wound healing, spreading of cells GO:0044319 ISS
    positive regulation of cilium assembly GO:0045724 ISS
    regulation of axonogenesis GO:0050770 IBA
    regulation of axonogenesis GO:0050770 ISS
    regulation of small GTPase mediated signal transduction GO:0051056 TAS
    neuron projection guidance GO:0097485 ISS
Subcellular Localization
    nucleus GO:0005634 IEA
    cytosol GO:0005829 IBA
    cytosol GO:0005829 TAS
    plasma membrane GO:0005886 IEA
    actin cytoskeleton GO:0015629 IEA
    ciliary basal body GO:0036064 ISS
 Experiment description of studies that identified ARHGAP35 in sEVs
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 207
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|HSP70|FLOT1
Enriched markers
✔
VDAC
Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23161513    
Organism Homo sapiens
Experiment description Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS.
Authors "Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ."
Journal name Mol Cell Proteomics
Publication year 2012
Sample Colorectal cancer cells
Sample name DKO-1
Isolation/purification methods Differential centrifugation
Filtration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
6
Experiment ID 208
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|HSP70|FLOT1
Enriched markers
✔
VDAC
Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23161513    
Organism Homo sapiens
Experiment description Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS.
Authors "Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ."
Journal name Mol Cell Proteomics
Publication year 2012
Sample Colorectal cancer cells
Sample name Dks-8
Isolation/purification methods Differential centrifugation
Filtration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
7
Experiment ID 209
MISEV standards
✘ Biophysical techniques
✔
TSG101|HSP70|FLOT1
Enriched markers
✔
VDAC
Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23161513    
Organism Homo sapiens
Experiment description Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS.
Authors "Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ."
Journal name Mol Cell Proteomics
Publication year 2012
Sample Colorectal cancer cells
Sample name DLD-1
Isolation/purification methods Differential centrifugation
Filtration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
8
Experiment ID 226
MISEV standards
✔
EM
Biophysical techniques
✔
GAPDH|CD9|FLOT1
Enriched markers
✔
LMNA|H2AFX|ATP5A1|TOMM20
Negative markers
✘ Particle analysis
Identified molecule mRNA
Identification method Small RNA sequencing (Illumina HiSeq 2000 (Solexa)
PubMed ID 26027894    
Organism Homo sapiens
Experiment description "Quantitative and qualitative analysis of small RNAs in human endothelial cells and exosomes provides insights into localized RNA processing, degradation and sorting"
Authors "Bas W. M. van Balkom, Almut S. Eisele, D. Michiel Pegtel, Sander Bervoets, Marianne C. Verhaar"
Journal name Journal of Extracellular Vesicles
Publication year 2015
Sample Endothelial cells
Sample name HMEC-1
Isolation/purification methods Differential ultracentrifugation
Sucrose density gradient
Flotation density 1.10 g/mL
Molecules identified in the study miRNA
Protein
snoRNA
lncRNA
yRNA
snRNA
mRNA
ncRNA
mtRNA
vtRNA
scaRNA
lincRNA
Methods used in the study Small RNA sequencing (Illumina HiSeq 2000 (Solexa)
Western blotting
9
Experiment ID 488
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD81|CD63|GAPDH|SDCBP|LAMP1|TFRC|UCHL1|FLOT2|LAMP2|FLOT1|ICAM1|RAB5B|CD151|RAB35|TSG101|RAB5A|CD82
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Homo sapiens
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Mesenchymal stem cells
Sample name UCMSC
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
10
Experiment ID 224
MISEV standards
✔
EM|AFM
Biophysical techniques
✔
Alix|TSG101|CD63|CD81
Enriched markers
✔
GOLGA2
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25944692    
Organism Homo sapiens
Experiment description Proteogenomic analysis reveals exosomes are more oncogenic than ectosomes
Authors "Keerthikumar S, Gangoda L, Liem M, Fonseka P, Atukorala I, Ozcitti C, Mechler A, Adda CG, Ang CS, Mathivanan S"
Journal name Oncotarget
Publication year 2015
Sample Neuroblastoma cells
Sample name SH-SY5Y
Isolation/purification methods Differential centrifugation
Ultracentrifugation
OptiPrep density gradient
Flotation density 1.10 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
Western blotting
 Protein-protein interactions for ARHGAP35
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 CBS 875
Affinity Capture-MS Homo sapiens
2 PA2G4 5036
Affinity Capture-MS Homo sapiens
3 RASA1 5921
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-MS Homo sapiens
4 FTL 2512
Affinity Capture-MS Homo sapiens
5 NME2 4831
Affinity Capture-MS Homo sapiens
6 NUDT21 11051
Affinity Capture-MS Homo sapiens
7 MECP2 4204
Affinity Capture-MS Homo sapiens
8 CIRBP 1153
Affinity Capture-MS Homo sapiens
9 GLOD4 51031
Affinity Capture-MS Homo sapiens
10 PRPF4B 8899
Affinity Capture-MS Homo sapiens
11 GRB2 2885
Affinity Capture-Western Homo sapiens
12 FAM167A  
Affinity Capture-MS Homo sapiens
13 MTPN 136319
Affinity Capture-MS Homo sapiens
14 RND1  
Affinity Capture-Western Homo sapiens
15 BAG2 9532
Affinity Capture-MS Homo sapiens
16 TCERG1 10915
Co-fractionation Homo sapiens
17 STOML2 30968
Affinity Capture-MS Homo sapiens
18 PFN1 5216
Proximity Label-MS Homo sapiens
19 PRDX2 7001
Affinity Capture-MS Homo sapiens
20 ACTC1 70
Proximity Label-MS Homo sapiens
21 SSSCA1 10534
Affinity Capture-MS Homo sapiens
22 HSPA12B 116835
Affinity Capture-MS Homo sapiens
23 MIB1 57534
Proximity Label-MS Homo sapiens
24 TRIM65 201292
Co-localization Homo sapiens
25 SF1 7536
Affinity Capture-MS Homo sapiens
26 SQSTM1 8878
Proximity Label-MS Homo sapiens
27 PLEKHA2 59339
Affinity Capture-MS Homo sapiens
28 DUT 1854
Affinity Capture-MS Homo sapiens
29 CSTB 1476
Affinity Capture-MS Homo sapiens
30 CFL1 1072
Affinity Capture-MS Homo sapiens
31 RAC3 5881
Affinity Capture-MS Homo sapiens
32 UCHL3 7347
Affinity Capture-MS Homo sapiens
33 BOLA2 552900
Affinity Capture-MS Homo sapiens
34 KIAA1598 57698
Co-fractionation Homo sapiens
35 CDC42 998
Reconstituted Complex Homo sapiens
36 DNAJA2 10294
Affinity Capture-MS Homo sapiens
37 RND3 390
Affinity Capture-Western Homo sapiens
38 C12orf74  
Affinity Capture-MS Homo sapiens
39 RND2  
Affinity Capture-Western Homo sapiens
40 CHCHD3 54927
Affinity Capture-MS Homo sapiens
41 PSPC1 55269
Affinity Capture-MS Homo sapiens
42 RPA3 6119
Proximity Label-MS Homo sapiens
43 SNRNP70 6625
Affinity Capture-MS Homo sapiens
44 HMGN1  
Affinity Capture-MS Homo sapiens
45 NTRK1 4914
Affinity Capture-MS Homo sapiens
46 AAMDC  
Affinity Capture-MS Homo sapiens
47 GOLGA1  
Proximity Label-MS Homo sapiens
48 SRSF2 6427
Affinity Capture-MS Homo sapiens
49 SERPINB2 5055
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
50 HSPA1A 3303
Affinity Capture-MS Homo sapiens
51 C14orf166 51637
Affinity Capture-MS Homo sapiens
52 MOB2 81532
Affinity Capture-MS Homo sapiens
53 ACTB 60
Proximity Label-MS Homo sapiens
54 BCL6  
Affinity Capture-MS Homo sapiens
55 MCTS1 28985
Affinity Capture-MS Homo sapiens
56 RHOB 388
Proximity Label-MS Homo sapiens
Affinity Capture-MS Homo sapiens
57 RAC1 5879
FRET Homo sapiens
Reconstituted Complex Homo sapiens
58 UBA6 55236
Co-fractionation Homo sapiens
59 HSPA12A 259217
Affinity Capture-MS Homo sapiens
60 RHOA 387
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
FRET Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-MS Homo sapiens
61 KRT31 3881
Two-hybrid Homo sapiens
62 SF3B3 23450
Affinity Capture-MS Homo sapiens
63 KIAA1211L 343990
Affinity Capture-MS Homo sapiens
64 Arhgap28  
Affinity Capture-MS Mus musculus
65 KLHL14 57565
Affinity Capture-MS Homo sapiens
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