Gene description for CBS
Gene name cystathionine-beta-synthase
Gene symbol CBS
Other names/aliases HIP4
Species Homo sapiens
 Database cross references - CBS
ExoCarta ExoCarta_875
Entrez Gene 875
HGNC 1550
MIM 613381
UniProt P35520  
 CBS identified in exosomes derived from the following tissue/cell type
Hepatocytes 26054723    
Ovarian cancer cells 23333927    
Ovarian cancer cells 23333927    
 Gene ontology annotations for CBS
Molecular Function
    cystathionine beta-synthase activity GO:0004122 TAS
    protein homodimerization activity GO:0042803 IDA
    metal ion binding GO:0046872 IEA
    nitrite reductase (NO-forming) activity GO:0050421 IDA
    protein binding GO:0005515 IPI
    nitric oxide binding GO:0070026 IDA
    modified amino acid binding GO:0072341 IDA
    ubiquitin protein ligase binding GO:0031625 IPI
    enzyme binding GO:0019899 IPI
    oxygen binding GO:0019825 IDA
    carbon monoxide binding GO:0070025 IDA
    S-adenosyl-L-methionine binding GO:1904047 IDA
    heme binding GO:0020037 IMP
    pyridoxal phosphate binding GO:0030170 IDA
    identical protein binding GO:0042802 IPI
Biological Process
    oxidation-reduction process GO:0055114 IDA
    hydrogen sulfide biosynthetic process GO:0070814 ISS
    L-serine catabolic process GO:0006565 IDA
    L-cysteine catabolic process GO:0019448 IDA
    DNA protection GO:0042262 IMP
    cysteine biosynthetic process via cystathionine GO:0019343 IBA
    cellular nitrogen compound metabolic process GO:0034641 TAS
    L-serine metabolic process GO:0006563 IDA
    cysteine biosynthetic process from serine GO:0006535 IEA
    cysteine biosynthetic process GO:0019344 IDA
    small molecule metabolic process GO:0044281 TAS
    transsulfuration GO:0019346 TAS
    homocysteine catabolic process GO:0043418 IDA
    sulfur amino acid metabolic process GO:0000096 TAS
    homocysteine metabolic process GO:0050667 IDA
Subcellular Localization
    nucleolus GO:0005730 IDA
    intracellular membrane-bounded organelle GO:0043231 IDA
    cytoplasm GO:0005737 IBA
    cytosol GO:0005829 TAS
    nucleus GO:0005634 IDA
 Experiment description of studies that identified CBS in exosomes
1
Experiment ID 237
ISEV standards
✔
EM
EV Biophysical techniques
✔
TSG101|Alix|HSC70|GAPDH
EV Cytosolic markers
✘ EV Membrane markers
✔
HSP90B1
EV Negative markers
✔
qNano
EV Particle analysis
Identified molecule mRNA
Identification method RNA Sequencing
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocytes
Sample name MIHA
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RNA Sequencing
2
Experiment ID 211
ISEV standards
✔
EM
EV Biophysical techniques
✔
TSG101|Alix
EV Cytosolic markers
✔
EpCAM|TFRC
EV Membrane markers
✔
cytochrome c|GOLGA2
EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K.
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name IGROV1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
3
Experiment ID 212
ISEV standards
✔
CEM
EV Biophysical techniques
✔
TSG101|Alix
EV Cytosolic markers
✔
EpCAM|TFRC
EV Membrane markers
✔
Cytochrome C|GOLGA2
EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K.
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name OVCAR-3
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for CBS
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 CBS 875
Invitro Homo sapiens
Invitro Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Invitro Homo sapiens
Invitro Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
2 HID1 283987
Two-hybrid Homo sapiens
3 FXR2 9513
Two-hybrid Homo sapiens
4 VTA1 51534
Two-hybrid Homo sapiens
View the network image/svg+xml
 Pathways in which CBS is involved
PathwayEvidenceSource
Cysteine formation from homocysteine TAS Reactome





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