Gene description for UBE3B
Gene name ubiquitin protein ligase E3B
Gene symbol UBE3B
Other names/aliases BPIDS
KOS
Species Homo sapiens
 Database cross references - UBE3B
ExoCarta ExoCarta_89910
Vesiclepedia VP_89910
Entrez Gene 89910
HGNC 13478
MIM 608047
UniProt Q7Z3V4  
 UBE3B identified in exosomes derived from the following tissue/cell type
Mesenchymal stem cells 36408942    
Thymus 23844026    
 Gene ontology annotations for UBE3B
Molecular Function
    protein binding GO:0005515 IPI
    ubiquitin protein ligase activity GO:0061630 IBA
    ubiquitin protein ligase activity GO:0061630 IDA
Biological Process
    protein polyubiquitination GO:0000209 IBA
    ubiquitin-dependent protein catabolic process GO:0006511 IBA
    regulation of postsynapse assembly GO:0150052 IEA
Subcellular Localization
    mitochondrion GO:0005739 IDA
    postsynaptic density GO:0014069 IEA
    glutamatergic synapse GO:0098978 IEA
 Experiment description of studies that identified UBE3B in exosomes
1
Experiment ID 488
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD81|CD63|GAPDH|SDCBP|LAMP1|TFRC|UCHL1|FLOT2|LAMP2|FLOT1|ICAM1|RAB5B|CD151|RAB35|TSG101|RAB5A|CD82
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Homo sapiens
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Mesenchymal stem cells
Sample name UCMSC
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 217
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|CD81|CD9|CD63
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23844026    
Organism Homo sapiens
Experiment description Characterization of human thymic exosomes.
Authors "Skogberg G, Gudmundsdottir J, van der Post S, Sandstrom K, Bruhn S, Benson M, Mincheva-Nilsson L, Baranov V, Telemo E, Ekwall O."
Journal name PLoS One
Publication year 2013
Sample Thymus
Sample name Normal-Thymus
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for UBE3B
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 CBS 875
Co-fractionation Homo sapiens
2 CALML3 810
Affinity Capture-MS Homo sapiens
3 UBE3B 89910
Biochemical Activity Homo sapiens
Biochemical Activity Homo sapiens
4 HRAS 3265
Reconstituted Complex Homo sapiens
5 VPS28 51160
Affinity Capture-MS Homo sapiens
6 VAMP8 8673
Affinity Capture-MS Homo sapiens
7 AGPAT9  
Affinity Capture-MS Homo sapiens
8 LMNB1 4001
Affinity Capture-MS Homo sapiens
9 NAPA 8775
Affinity Capture-MS Homo sapiens
10 CALM2 805
Affinity Capture-MS Homo sapiens
11 IQCB1  
Affinity Capture-MS Homo sapiens
12 IFI30  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
13 USP1 7398
Affinity Capture-MS Homo sapiens
14 VAMP4 8674
Affinity Capture-MS Homo sapiens
15 Oxnad1  
Affinity Capture-MS Mus musculus
16 SHFM1 7979
Affinity Capture-MS Homo sapiens
17 CALM1 801
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
18 UBB 7314
Affinity Capture-MS Homo sapiens
19 DLK1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
20 SNF8 11267
Affinity Capture-MS Homo sapiens
21 POLR2A 5430
Co-fractionation Homo sapiens
22 BET1L 51272
Affinity Capture-MS Homo sapiens
23 CAPZB 832
Affinity Capture-MS Homo sapiens
24 TOP2A 7153
Affinity Capture-MS Homo sapiens
25 EDC4 23644
Affinity Capture-MS Homo sapiens
26 MAN1A2 10905
Affinity Capture-MS Homo sapiens
27 MYL4 4635
Affinity Capture-MS Homo sapiens
28 SEC16A 9919
Affinity Capture-MS Homo sapiens
29 GPR182  
Affinity Capture-MS Homo sapiens
30 ORAI1 84876
Affinity Capture-MS Homo sapiens
31 LIG1 3978
Affinity Capture-MS Homo sapiens
32 ELOVL1 64834
Affinity Capture-MS Homo sapiens
33 S100B 6285
Affinity Capture-MS Homo sapiens
34 NAPG 8774
Affinity Capture-MS Homo sapiens
35 SCD 6319
Affinity Capture-MS Homo sapiens
36 RAC1 5879
Affinity Capture-MS Homo sapiens
37 CALM3 808
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Proximity Label-MS Homo sapiens
Affinity Capture-MS Homo sapiens
38 EFCAB2  
Affinity Capture-MS Homo sapiens
39 MYL12B 103910
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
View the network image/svg+xml



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