Gene description for LIG1
Gene name ligase I, DNA, ATP-dependent
Gene symbol LIG1
Other names/aliases -
Species Homo sapiens
 Database cross references - LIG1
ExoCarta ExoCarta_3978
Entrez Gene 3978
HGNC 6598
MIM 126391
UniProt P18858  
 LIG1 identified in exosomes derived from the following tissue/cell type
Ovarian cancer cells 23333927    
Ovarian cancer cells 23333927    
 Gene ontology annotations for LIG1
Molecular Function
    DNA ligase activity GO:0003909 IMP
    ATP binding GO:0005524 IEA
    DNA ligase (ATP) activity GO:0003910 IBA
    DNA binding GO:0003677 IEA
    metal ion binding GO:0046872 IEA
Biological Process
    response to hydrogen peroxide GO:0042542 IEA
    telomere maintenance via semi-conservative replication GO:0032201 TAS
    double-strand break repair via nonhomologous end joining GO:0006303 IEA
    DNA ligation GO:0006266 IMP
    telomere maintenance via recombination GO:0000722 TAS
    anatomical structure morphogenesis GO:0009653 TAS
    cell division GO:0051301 IEA
    double-strand break repair via homologous recombination GO:0000724 TAS
    telomere maintenance GO:0000723 TAS
    DNA repair GO:0006281 TAS
    removal of RNA primer involved in mitotic DNA replication GO:1903469 IBA
    nucleotide-excision repair, DNA gap filling GO:0006297 TAS
    DNA metabolic process GO:0006259 TAS
    transcription-coupled nucleotide-excision repair GO:0006283 TAS
    mismatch repair GO:0006298 TAS
    base-excision repair GO:0006284 TAS
    double-strand break repair GO:0006302 TAS
    DNA biosynthetic process GO:0071897 IEA
    nucleotide-excision repair GO:0006289 TAS
    V(D)J recombination GO:0033151 IDA
    Okazaki fragment processing involved in mitotic DNA replication GO:1903461 IBA
    lagging strand elongation GO:0006273 IBA
    DNA strand elongation involved in DNA replication GO:0006271 TAS
    mitotic cell cycle GO:0000278 TAS
    DNA ligation involved in DNA repair GO:0051103 IBA
Subcellular Localization
    Golgi apparatus GO:0005794 IDA
    nucleus GO:0005634 TAS
    mitochondrion GO:0005739 IBA
    cytoplasm GO:0005737 IBA
    intracellular membrane-bounded organelle GO:0043231 IDA
    nucleoplasm GO:0005654 TAS
 Experiment description of studies that identified LIG1 in exosomes
1
Experiment ID 211
ISEV standards
EM
EV Biophysical techniques
TSG101|Alix
EV Cytosolic markers
EpCAM|TFRC
EV Membrane markers
cytochrome c|GOLGA2
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K.
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name IGROV1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
2
Experiment ID 212
ISEV standards
CEM
EV Biophysical techniques
TSG101|Alix
EV Cytosolic markers
EpCAM|TFRC
EV Membrane markers
Cytochrome C|GOLGA2
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K.
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name OVCAR-3
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for LIG1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 MRE11A 4361
Two-hybrid Homo sapiens
2 PCNA 5111
Invitro Homo sapiens
View the network image/svg+xml



Perform bioinformatics analysis of your extracellular vesicle data set using FunRich, a open access standalone tool. NEW UPDATED VERSION OF FunRich available for download (12/09/2016) from here