Gene description for LIG1
Gene name ligase I, DNA, ATP-dependent
Gene symbol LIG1
Other names/aliases -
Species Homo sapiens
 Database cross references - LIG1
ExoCarta ExoCarta_3978
Vesiclepedia VP_3978
Entrez Gene 3978
HGNC 6598
MIM 126391
UniProt P18858  
 LIG1 identified in exosomes derived from the following tissue/cell type
Mesenchymal stem cells 36408942    
Ovarian cancer cells 23333927    
Ovarian cancer cells 23333927    
 Gene ontology annotations for LIG1
Molecular Function
    DNA ligase activity GO:0003909 IMP
    ATP binding GO:0005524 IEA
    DNA ligase (ATP) activity GO:0003910 IBA
    DNA binding GO:0003677 IEA
    metal ion binding GO:0046872 IEA
Biological Process
    response to hydrogen peroxide GO:0042542 IEA
    telomere maintenance via semi-conservative replication GO:0032201 TAS
    double-strand break repair via nonhomologous end joining GO:0006303 IEA
    DNA ligation GO:0006266 IMP
    telomere maintenance via recombination GO:0000722 TAS
    anatomical structure morphogenesis GO:0009653 TAS
    cell division GO:0051301 IEA
    double-strand break repair via homologous recombination GO:0000724 TAS
    telomere maintenance GO:0000723 TAS
    DNA repair GO:0006281 TAS
    removal of RNA primer involved in mitotic DNA replication GO:1903469 IBA
    nucleotide-excision repair, DNA gap filling GO:0006297 TAS
    DNA metabolic process GO:0006259 TAS
    transcription-coupled nucleotide-excision repair GO:0006283 TAS
    mismatch repair GO:0006298 TAS
    base-excision repair GO:0006284 TAS
    double-strand break repair GO:0006302 TAS
    DNA biosynthetic process GO:0071897 IEA
    nucleotide-excision repair GO:0006289 TAS
    V(D)J recombination GO:0033151 IDA
    Okazaki fragment processing involved in mitotic DNA replication GO:1903461 IBA
    lagging strand elongation GO:0006273 IBA
    DNA strand elongation involved in DNA replication GO:0006271 TAS
    mitotic cell cycle GO:0000278 TAS
    DNA ligation involved in DNA repair GO:0051103 IBA
Subcellular Localization
    Golgi apparatus GO:0005794 IDA
    nucleus GO:0005634 TAS
    mitochondrion GO:0005739 IBA
    cytoplasm GO:0005737 IBA
    intracellular membrane-bounded organelle GO:0043231 IDA
    nucleoplasm GO:0005654 TAS
 Experiment description of studies that identified LIG1 in exosomes
1
Experiment ID 488
MISEV standards
✔
EM
EV Biophysical techniques
✔
CD9|CD81|CD63|GAPDH|SDCBP|LAMP1|TFRC|UCHL1|FLOT2|LAMP2|FLOT1|ICAM1|RAB5B|CD151|RAB35|TSG101|RAB5A|CD82
EV Enriched markers
✔
CANX
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Homo sapiens
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ.
Journal name Proteomics
Publication year 2023
Sample Mesenchymal stem cells
Sample name UCMSC
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 211
MISEV standards
✔
EM
EV Biophysical techniques
✔
TSG101|Alix|EpCAM|TFRC
EV Enriched markers
✔
cytochrome c|GOLGA2
EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K.
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name IGROV1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
3
Experiment ID 212
MISEV standards
✔
CEM
EV Biophysical techniques
✔
TSG101|Alix|EpCAM|TFRC
EV Enriched markers
✔
Cytochrome C|GOLGA2
EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K.
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name OVCAR-3
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for LIG1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 MRE11A 4361
Two-hybrid Homo sapiens
2 PCNA 5111
Invitro Homo sapiens
View the network image/svg+xml



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