Gene description for NT5DC2
Gene name 5'-nucleotidase domain containing 2
Gene symbol NT5DC2
Other names/aliases -
Species Homo sapiens
 Database cross references - NT5DC2
ExoCarta ExoCarta_64943
Vesiclepedia VP_64943
Entrez Gene 64943
HGNC 25717
UniProt Q9H857  
 NT5DC2 identified in exosomes derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Hepatocellular carcinoma cells 26054723    
Hepatocellular carcinoma cells 26054723    
Hepatocellular carcinoma cells 26054723    
Hepatocytes 26054723    
Mesenchymal stem cells 36408942    
 Gene ontology annotations for NT5DC2
Molecular Function
    5'-nucleotidase activity GO:0008253 IBA
    metal ion binding GO:0046872 IEA
Biological Process
    negative regulation of peptidyl-serine phosphorylation GO:0033137 ISS
    negative regulation of catecholamine metabolic process GO:0045914 ISS
    negative regulation of oxidoreductase activity GO:0051354 ISS
    negative regulation of dopamine biosynthetic process GO:1903180 ISS
Subcellular Localization
    cytoplasm GO:0005737 ISS
    mitochondrion GO:0005739 HTP
 Experiment description of studies that identified NT5DC2 in exosomes
1
Experiment ID 489
MISEV standards
EM
Biophysical techniques
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
Canx
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
EM
Biophysical techniques
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
Canx
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
EM
Biophysical techniques
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
Canx
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
EM
Biophysical techniques
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
Canx
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 234
MISEV standards
EM
Biophysical techniques
TSG101|Alix|HSC70|GAPDH
Enriched markers
HSP90B1
Negative markers
qNano
Particle analysis
Identified molecule mRNA
Identification method RNA Sequencing
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors "He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N"
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocellular carcinoma cells
Sample name HKCI-C3
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RT-PCR
RNA Sequencing
6
Experiment ID 235
MISEV standards
EM
Biophysical techniques
TSG101|Alix|HSC70|GAPDH
Enriched markers
HSP90B1
Negative markers
qNano
Particle analysis
Identified molecule mRNA
Identification method RNA Sequencing
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors "He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N"
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocellular carcinoma cells
Sample name HKCI-8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RT-PCR
RNA Sequencing
7
Experiment ID 236
MISEV standards
EM
Biophysical techniques
TSG101|Alix|HSC70|GAPDH
Enriched markers
HSP90B1
Negative markers
qNano
Particle analysis
Identified molecule mRNA
Identification method RNA Sequencing
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors "He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N"
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocellular carcinoma cells
Sample name MHCC97L
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RT-PCR
RNA Sequencing
8
Experiment ID 237
MISEV standards
EM
Biophysical techniques
TSG101|Alix|HSC70|GAPDH
Enriched markers
HSP90B1
Negative markers
qNano
Particle analysis
Identified molecule mRNA
Identification method RNA Sequencing
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors "He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N"
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocytes
Sample name MIHA
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RNA Sequencing
9
Experiment ID 488
MISEV standards
EM
Biophysical techniques
CD9|CD81|CD63|GAPDH|SDCBP|LAMP1|TFRC|UCHL1|FLOT2|LAMP2|FLOT1|ICAM1|RAB5B|CD151|RAB35|TSG101|RAB5A|CD82
Enriched markers
CANX
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Homo sapiens
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Mesenchymal stem cells
Sample name UCMSC
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
 Protein-protein interactions for NT5DC2
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 UBE2H 7328
Affinity Capture-MS Homo sapiens
2 PDHA1 5160
Proximity Label-MS Homo sapiens
3 CISD3 284106
Affinity Capture-MS Homo sapiens
4 CLPP 8192
Proximity Label-MS Homo sapiens
5 LYRM5  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
6 CS 1431
Proximity Label-MS Homo sapiens
Proximity Label-MS Homo sapiens
7 MRM1  
Affinity Capture-MS Homo sapiens
8 PLEKHA4 57664
Affinity Capture-MS Homo sapiens
9 PMPCB 9512
Proximity Label-MS Homo sapiens
10 TACO1  
Proximity Label-MS Homo sapiens
11 TERF2  
Affinity Capture-MS Homo sapiens
12 HSPB2  
Two-hybrid Homo sapiens
13 MCM2 4171
Affinity Capture-MS Homo sapiens
14 LRPPRC 10128
Proximity Label-MS Homo sapiens
15 KIAA1598 57698
Co-fractionation Homo sapiens
16 TBRG4 9238
Proximity Label-MS Homo sapiens
17 MRPL11 65003
Proximity Label-MS Homo sapiens
18 MTERF3  
Proximity Label-MS Homo sapiens
19 KIF14 9928
Affinity Capture-MS Homo sapiens
20 TIPRL 261726
Co-fractionation Homo sapiens
21 MTRF1L  
Proximity Label-MS Homo sapiens
22 NT5DC3 51559
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
23 MDH2 4191
Proximity Label-MS Homo sapiens
24 TUFM 7284
Proximity Label-MS Homo sapiens
25 IL25  
Affinity Capture-MS Homo sapiens
26 NME4 4833
Affinity Capture-MS Homo sapiens
27 RPA2 6118
Proximity Label-MS Homo sapiens
28 TOLLIP 54472
Affinity Capture-MS Homo sapiens
29 TRIM66  
Affinity Capture-MS Homo sapiens
30 TRUB2  
Proximity Label-MS Homo sapiens
31 CCDC109B 55013
Proximity Label-MS Homo sapiens
32 MTG2  
Proximity Label-MS Homo sapiens
33 C6orf203  
Proximity Label-MS Homo sapiens
34 LIG1 3978
Co-fractionation Homo sapiens
35 CUL3 8452
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
36 TRIM33 51592
Affinity Capture-MS Homo sapiens
37 FASTKD5  
Proximity Label-MS Homo sapiens
38 EGFR 1956
Affinity Capture-MS Homo sapiens
39 C9orf78 51759
Affinity Capture-MS Homo sapiens
40 COX8A  
Proximity Label-MS Homo sapiens
41 PARK2  
Affinity Capture-MS Homo sapiens
42 XPNPEP3 63929
Affinity Capture-MS Homo sapiens
43 PMPCA 23203
Proximity Label-MS Homo sapiens
44 C21orf33  
Proximity Label-MS Homo sapiens
45 SSBP1 6742
Proximity Label-MS Homo sapiens
46 HSCB 150274
Proximity Label-MS Homo sapiens
47 DUSP13  
Affinity Capture-MS Homo sapiens
48 C7orf55  
Affinity Capture-MS Homo sapiens
49 KLHDC9  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
50 CAPZB 832
Affinity Capture-MS Homo sapiens
51 BIRC6 57448
Affinity Capture-MS Homo sapiens
52 MTIF2 4528
Proximity Label-MS Homo sapiens
53 C1QBP 708
Proximity Label-MS Homo sapiens
54 GFM1 85476
Proximity Label-MS Homo sapiens
55 AARS2  
Proximity Label-MS Homo sapiens
56 RPA3 6119
Proximity Label-MS Homo sapiens
57 NTRK1 4914
Affinity Capture-MS Homo sapiens
58 COQ9  
Affinity Capture-MS Homo sapiens
59 CSNK2A2 1459
Affinity Capture-MS Homo sapiens
60 TSFM 10102
Proximity Label-MS Homo sapiens
61 TSNAX 7257
Affinity Capture-MS Homo sapiens
62 POLDIP2 26073
Affinity Capture-MS Homo sapiens
63 BRD1 23774
Affinity Capture-MS Homo sapiens
64 ACAD9 28976
Proximity Label-MS Homo sapiens
65 HINT2 84681
Proximity Label-MS Homo sapiens
66 UFL1 23376
Affinity Capture-MS Homo sapiens
67 POU5F1  
Affinity Capture-MS Homo sapiens
68 EMILIN1 11117
Affinity Capture-MS Homo sapiens
69 ZNF444  
Affinity Capture-MS Homo sapiens
70 METTL17  
Proximity Label-MS Homo sapiens
71 MAD2L2 10459
Proximity Label-MS Homo sapiens
Affinity Capture-MS Homo sapiens
72 ATOH1  
Affinity Capture-MS Homo sapiens
73 MTG1  
Proximity Label-MS Homo sapiens
74 AUH 549
Proximity Label-MS Homo sapiens
75 METTL15  
Proximity Label-MS Homo sapiens
76 SURF6  
Affinity Capture-MS Homo sapiens
77 MCUR1 63933
Proximity Label-MS Homo sapiens
78 NR4A1  
Affinity Capture-MS Homo sapiens
79 EXD2  
Proximity Label-MS Homo sapiens
80 TULP3 7289
Affinity Capture-MS Homo sapiens
81 NDUFS7 374291
Affinity Capture-MS Homo sapiens
View the network image/svg+xml
 Pathways in which NT5DC2 is involved
No pathways found





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