Gene description for ARHGEF1
Gene name Rho guanine nucleotide exchange factor (GEF) 1
Gene symbol ARHGEF1
Other names/aliases GEF1
LBCL2
LSC
P115-RHOGEF
SUB1.5
Species Homo sapiens
 Database cross references - ARHGEF1
ExoCarta ExoCarta_9138
Entrez Gene 9138
HGNC 681
MIM 601855
UniProt Q92888  
 ARHGEF1 identified in exosomes derived from the following tissue/cell type
Mesenchymal stem cells Unpublished / Not applicable
Prostate cancer cells 25844599    
Thymus 23844026    
 Gene ontology annotations for ARHGEF1
Molecular Function
    GTPase activator activity GO:0005096 IEA
    protein binding GO:0005515 IPI
    Rho guanyl-nucleotide exchange factor activity GO:0005089 IEA
    poly(A) RNA binding GO:0044822 IDA
Biological Process
    negative regulation of axonogenesis GO:0050771 TAS
    cell proliferation GO:0008283 TAS
    positive regulation of GTPase activity GO:0043547 TAS
    neurotrophin TRK receptor signaling pathway GO:0048011 TAS
    regulation of axonogenesis GO:0050770 TAS
    termination of G-protein coupled receptor signaling pathway GO:0038032 IEA
    Rho protein signal transduction GO:0007266 TAS
Subcellular Localization
    cytoplasm GO:0005737 IDA
    plasma membrane GO:0005886 TAS
    cytosol GO:0005829 TAS
 Experiment description of studies that identified ARHGEF1 in exosomes
1
Experiment ID 126
ISEV standards
✘ EV Biophysical techniques
✔
GAPDH
EV Cytosolic markers
✘ EV Membrane markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry [LTQ-FT Ultra]
PubMed ID Unpublished / Not applicable
Organism Homo sapiens
Experiment description Mesenchymal Stem Cell Exosomes: The Future MSC-based Therapy?
Authors Ruenn Chai Lai, Ronne Wee Yeh Yeo, Soon Sim Tan, Bin Zhang, Yijun Yin, Newman Siu Kwan Sze, Andre Choo, and Sai Kiang Lim
Journal name Mesenchymal Stem Cell Therapy
Publication year 2011
Sample Mesenchymal stem cells
Sample name huES9.E1
Isolation/purification methods HPLC
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Antobody array
Mass spectrometry
2
Experiment ID 275
ISEV standards
✔
EM
EV Biophysical techniques
✔
TSG101|Alix|RAB5A
EV Cytosolic markers
✔
CD9|CD82|CD63|CD81
EV Membrane markers
✔
AIF
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25844599    
Organism Homo sapiens
Experiment description Molecular profiling of prostate cancer derived exosomes may reveal a predictive signature for response to docetaxel.
Authors Kharaziha P, Chioureas D, Rutishauser D, Baltatzis G, Lennartsson L, Fonseca P, Azimi A, Hultenby K, Zubarev R, Ullen A, Yachnin J, Nilsson S, Panaretakis T.
Journal name Oncotarget
Publication year 2015
Sample Prostate cancer cells
Sample name DU145 - Docetaxel sensitive
Isolation/purification methods Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.12-1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry/Flow cytometry/Western blotting
3
Experiment ID 217
ISEV standards
✔
EM
EV Biophysical techniques
✔
TSG101
EV Cytosolic markers
✔
CD81|CD9|CD63
EV Membrane markers
✘ EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23844026    
Organism Homo sapiens
Experiment description Characterization of human thymic exosomes.
Authors Skogberg G, Gudmundsdottir J, van der Post S, Sandstrom K, Bruhn S, Benson M, Mincheva-Nilsson L, Baranov V, Telemo E, Ekwall O.
Journal name PLoS One
Publication year 2013
Sample Thymus
Sample name Normal-Thymus
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for ARHGEF1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 ATR  
Protein-peptide Homo sapiens
2 ARHGEF1 9138
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
3 GNA13 10672
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
4 CD44 960
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
5 GNA12 2768
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
6 HRAS 3265
Biochemical Activity Homo sapiens
7 RHOA 387
Biochemical Activity Homo sapiens
8 RAP1A 5906
Biochemical Activity Homo sapiens
9 PIK3CA 5290
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
View the network image/svg+xml
 Pathways in which ARHGEF1 is involved
PathwayEvidenceSource
Axonal growth inhibition (RHOA activation) TAS Reactome
G alpha (12/13) signalling events TAS Reactome





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