Gene description for BLOC1S2
Gene name biogenesis of lysosomal organelles complex-1, subunit 2
Gene symbol BLOC1S2
Other names/aliases BLOS2
CEAP
CEAP11
Species Homo sapiens
 Database cross references - BLOC1S2
ExoCarta ExoCarta_282991
Vesiclepedia VP_282991
Entrez Gene 282991
HGNC 20984
MIM 609768
UniProt Q6QNY1  
 BLOC1S2 identified in exosomes derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Hepatocytes 26054723    
 Gene ontology annotations for BLOC1S2
Molecular Function
    protein binding GO:0005515 IPI
    gamma-tubulin binding GO:0043015 IBA
    gamma-tubulin binding GO:0043015 IDA
Biological Process
    microtubule nucleation GO:0007020 NAS
    anterograde axonal transport GO:0008089 ISS
    positive regulation of cell population proliferation GO:0008284 IDA
    extrinsic apoptotic signaling pathway via death domain receptors GO:0008625 IDA
    endosomal transport GO:0016197 IBA
    neuron projection development GO:0031175 ISS
    lysosome localization GO:0032418 IBA
    lysosome localization GO:0032418 IMP
    lysosome localization GO:0032418 NAS
    melanosome organization GO:0032438 NAS
    positive regulation of DNA-templated transcription GO:0045893 IDA
    anterograde synaptic vesicle transport GO:0048490 ISS
    regulation of endosome size GO:0051036 NAS
    platelet dense granule organization GO:0060155 NAS
    regulation of lysosome size GO:0062196 NAS
    organelle transport along microtubule GO:0072384 NAS
    mitochondrial outer membrane permeabilization GO:0097345 IDA
Subcellular Localization
    gamma-tubulin complex GO:0000930 IBA
    gamma-tubulin complex GO:0000930 IDA
    nucleus GO:0005634 IDA
    mitochondrion GO:0005739 IDA
    centrosome GO:0005813 IDA
    centrosome GO:0005813 IEA
    cytosol GO:0005829 IEA
    BLOC-1 complex GO:0031083 IBA
    BLOC-1 complex GO:0031083 IDA
    BLOC-1 complex GO:0031083 IPI
    cytoplasmic side of lysosomal membrane GO:0098574 NAS
    BORC complex GO:0099078 IBA
    BORC complex GO:0099078 IDA
    BORC complex GO:0099078 IPI
    axon cytoplasm GO:1904115 IEA
 Experiment description of studies that identified BLOC1S2 in exosomes
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 237
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|Alix|HSC70|GAPDH
Enriched markers
✔
HSP90B1
Negative markers
✔
qNano
Particle analysis
Identified molecule mRNA
Identification method RNA Sequencing
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors "He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N"
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocytes
Sample name MIHA
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RNA Sequencing
 Protein-protein interactions for BLOC1S2
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 LAMTOR5 10542
Affinity Capture-MS Homo sapiens
2 BRD2  
Affinity Capture-MS Homo sapiens
3 TMEM55B 90809
Affinity Capture-MS Homo sapiens
4 SNX7 51375
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
5 VAV1 7409
Affinity Capture-MS Homo sapiens
6 C8orf33 65265
Two-hybrid Homo sapiens
7 DCTN2 10540
Two-hybrid Homo sapiens
8 MEF2BNB  
Affinity Capture-MS Homo sapiens
9 BLOC1S4 55330
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
10 BLOC1S6  
Co-purification Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
11 CUL3 8452
Affinity Capture-MS Homo sapiens
12 IFT20 90410
Affinity Capture-MS Homo sapiens
13 CCNC  
Two-hybrid Homo sapiens
14 EXOC8 149371
Two-hybrid Homo sapiens
15 MEST 4232
Two-hybrid Homo sapiens
16 CCHCR1  
Two-hybrid Homo sapiens
17 TEX35  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
18 C15orf59  
Affinity Capture-MS Homo sapiens
19 GADD45G  
Two-hybrid Homo sapiens
20 CCDC146  
Two-hybrid Homo sapiens
21 HMOX2 3163
Two-hybrid Homo sapiens
22 TSKS  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
23 FAM9B  
Two-hybrid Homo sapiens
24 COG7 91949
Affinity Capture-MS Homo sapiens
25 IFT57 55081
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
26 LAMTOR2 28956
Affinity Capture-MS Homo sapiens
27 TRIML2  
Affinity Capture-MS Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
28 LAMTOR4 389541
Affinity Capture-MS Homo sapiens
29 IKBIP 121457
Affinity Capture-MS Homo sapiens
30 FAM161B  
Two-hybrid Homo sapiens
31 BFSP2  
Two-hybrid Homo sapiens
32 SNAPIN 23557
Two-hybrid Homo sapiens
Co-fractionation Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
Two-hybrid Homo sapiens
33 BLOC1S3 388552
Affinity Capture-Western Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
34 BCAS4  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
35 SSC5D 284297
Two-hybrid Homo sapiens
36 EXOC1 55763
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
37 TRIM69 140691
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
38 KRT27 342574
Affinity Capture-MS Homo sapiens
39 COG6 57511
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
40 PAM16  
Two-hybrid Homo sapiens
41 ARHGEF1 9138
Affinity Capture-MS Homo sapiens
42 C10orf32  
Affinity Capture-MS Homo sapiens
43 BLOC1S1  
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
44 DTNBP1  
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
45 BLOC1S5 63915
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
46 GEM  
Two-hybrid Homo sapiens
47 OSBPL9 114883
Affinity Capture-MS Homo sapiens
48 RAB7A 7879
Proximity Label-MS Homo sapiens
49 MYPOP  
Affinity Capture-MS Homo sapiens
50 SYBU  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
51 KXD1 79036
Affinity Capture-MS Homo sapiens
52 TRAF3IP1 26146
Affinity Capture-MS Homo sapiens
53 RDH11 51109
Two-hybrid Homo sapiens
54 ANGPT4  
Affinity Capture-MS Homo sapiens
55 ACTB 60
Affinity Capture-MS Homo sapiens
56 LAMTOR1 55004
Affinity Capture-MS Homo sapiens
57 SH2D4A 63898
Two-hybrid Homo sapiens
58 CCDC53 51019
Affinity Capture-MS Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
59 SCOC 60592
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
60 P4HA3 283208
Two-hybrid Homo sapiens
61 CTNNA3  
Two-hybrid Homo sapiens
62 WHAMMP3  
Affinity Capture-MS Homo sapiens
63 ARFIP2 23647
Two-hybrid Homo sapiens
64 BRK1 55845
Affinity Capture-MS Homo sapiens
65 WASH1 100287171
Two-hybrid Homo sapiens
66 ERP27  
Two-hybrid Homo sapiens
67 STX2 2054
Two-hybrid Homo sapiens
68 APP 351
Reconstituted Complex Homo sapiens
69 C17orf59 54785
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
70 KRT26 353288
Two-hybrid Homo sapiens
71 TNNT1  
Two-hybrid Homo sapiens
View the network image/svg+xml
 Pathways in which BLOC1S2 is involved
No pathways found





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