Gene description for DOCK4
Gene name dedicator of cytokinesis 4
Gene symbol DOCK4
Other names/aliases -
Species Homo sapiens
 Database cross references - DOCK4
ExoCarta ExoCarta_9732
Entrez Gene 9732
HGNC 19192
MIM 607679
UniProt Q8N1I0  
 DOCK4 identified in exosomes derived from the following tissue/cell type
Endothelial cells 26027894    
 Gene ontology annotations for DOCK4
Molecular Function
    Rac GTPase binding GO:0048365 IDA
    protein binding GO:0005515 IPI
    guanyl-nucleotide exchange factor activity GO:0005085 IEA
    SH3 domain binding GO:0017124 IEA
    receptor tyrosine kinase binding GO:0030971 IPI
    GTPase activator activity GO:0005096 IDA
    PDZ domain binding GO:0030165 IPI
Biological Process
    cell chemotaxis GO:0060326 IMP
    small GTPase mediated signal transduction GO:0007264 IEA
    positive regulation of GTPase activity GO:0043547 IEA
Subcellular Localization
    stereocilium GO:0032420 ISS
    cytosol GO:0005829 IDA
    membrane GO:0016020 IDA
    stereocilium bundle GO:0032421 ISS
    endomembrane system GO:0012505 IEA
 Experiment description of studies that identified DOCK4 in exosomes
1
Experiment ID 226
ISEV standards
EM
EV Biophysical techniques
GAPDH
EV Cytosolic markers
CD9|FLOT1
EV Membrane markers
LMNA|H2AFX|ATP5A1|TOMM20
EV Negative markers
EV Particle analysis
Identified molecule mRNA
Identification method Small RNA sequencing (Illumina HiSeq 2000 (Solexa)
PubMed ID 26027894    
Organism Homo sapiens
Experiment description Quantitative and qualitative analysis of small RNAs in human endothelial cells and exosomes provides insights into localized RNA processing, degradation and sorting
Authors Bas W. M. van Balkom, Almut S. Eisele, D. Michiel Pegtel, Sander Bervoets, Marianne C. Verhaar
Journal name Journal of Extracellular Vesicles
Publication year 2015
Sample Endothelial cells
Sample name HMEC-1
Isolation/purification methods Differential ultracentrifugation
Sucrose density gradient
Flotation density 1.10 g/mL
Molecules identified in the study miRNA
Protein
snoRNA
lncRNA
yRNA
snRNA
mRNA
ncRNA
mtRNA
vtRNA
scaRNA
lincRNA
Methods used in the study Small RNA sequencing (Illumina HiSeq 2000 (Solexa)
Western blotting
 Protein-protein interactions for DOCK4
  Protein Interactor ExoCarta ID Identification method PubMed Species
No interactions are found.
 Pathways in which DOCK4 is involved
PathwayEvidenceSource
Factors involved in megakaryocyte development and platelet production TAS Reactome





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