Gene description for DOCK4
Gene name dedicator of cytokinesis 4
Gene symbol DOCK4
Other names/aliases -
Species Homo sapiens
 Database cross references - DOCK4
ExoCarta ExoCarta_9732
Vesiclepedia VP_9732
Entrez Gene 9732
HGNC 19192
MIM 607679
UniProt Q8N1I0  
 DOCK4 identified in exosomes derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Endothelial cells 26027894    
Mesenchymal stem cells 36408942    
 Gene ontology annotations for DOCK4
Molecular Function
    guanyl-nucleotide exchange factor activity GO:0005085 IBA
    guanyl-nucleotide exchange factor activity GO:0005085 IDA
    GTPase activator activity GO:0005096 IEA
    protein binding GO:0005515 IPI
    SH3 domain binding GO:0017124 IEA
    PDZ domain binding GO:0030165 IPI
    receptor tyrosine kinase binding GO:0030971 IPI
    small GTPase binding GO:0031267 IBA
    small GTPase binding GO:0031267 IDA
Biological Process
    small GTPase-mediated signal transduction GO:0007264 IEA
    cell chemotaxis GO:0060326 IBA
    cell chemotaxis GO:0060326 IMP
    regulation of postsynapse assembly GO:0150052 IEA
    negative regulation of vascular associated smooth muscle contraction GO:1904694 IMP
    positive regulation of vascular associated smooth muscle cell migration GO:1904754 IMP
Subcellular Localization
    nucleolus GO:0005730 IDA
    cytoplasm GO:0005737 IBA
    Golgi apparatus GO:0005794 IDA
    cytosol GO:0005829 IDA
    cytosol GO:0005829 TAS
    plasma membrane GO:0005886 IBA
    plasma membrane GO:0005886 IDA
    membrane GO:0016020 IDA
    stereocilium GO:0032420 ISS
    stereocilium bundle GO:0032421 ISS
    postsynapse GO:0098794 IEA
    glutamatergic synapse GO:0098978 IEA
 Experiment description of studies that identified DOCK4 in exosomes
1
Experiment ID 489
MISEV standards
EM
Biophysical techniques
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
Canx
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
EM
Biophysical techniques
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
Canx
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
EM
Biophysical techniques
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
Canx
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
EM
Biophysical techniques
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
Canx
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 226
MISEV standards
EM
Biophysical techniques
GAPDH|CD9|FLOT1
Enriched markers
LMNA|H2AFX|ATP5A1|TOMM20
Negative markers
Particle analysis
Identified molecule mRNA
Identification method Small RNA sequencing (Illumina HiSeq 2000 (Solexa)
PubMed ID 26027894    
Organism Homo sapiens
Experiment description "Quantitative and qualitative analysis of small RNAs in human endothelial cells and exosomes provides insights into localized RNA processing, degradation and sorting"
Authors "Bas W. M. van Balkom, Almut S. Eisele, D. Michiel Pegtel, Sander Bervoets, Marianne C. Verhaar"
Journal name Journal of Extracellular Vesicles
Publication year 2015
Sample Endothelial cells
Sample name HMEC-1
Isolation/purification methods Differential ultracentrifugation
Sucrose density gradient
Flotation density 1.10 g/mL
Molecules identified in the study miRNA
Protein
snoRNA
lncRNA
yRNA
snRNA
mRNA
ncRNA
mtRNA
vtRNA
scaRNA
lincRNA
Methods used in the study Small RNA sequencing (Illumina HiSeq 2000 (Solexa)
Western blotting
6
Experiment ID 488
MISEV standards
EM
Biophysical techniques
CD9|CD81|CD63|GAPDH|SDCBP|LAMP1|TFRC|UCHL1|FLOT2|LAMP2|FLOT1|ICAM1|RAB5B|CD151|RAB35|TSG101|RAB5A|CD82
Enriched markers
CANX
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Homo sapiens
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Mesenchymal stem cells
Sample name UCMSC
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
 Protein-protein interactions for DOCK4
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 UBE2H 7328
Affinity Capture-MS Homo sapiens
2 GYPA  
Affinity Capture-MS Homo sapiens
3 GRK5 2869
Affinity Capture-MS Homo sapiens
4 ERMAP 114625
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
5 ARHGEF17 9828
Affinity Capture-MS Homo sapiens
6 PLEKHA4 57664
Affinity Capture-MS Homo sapiens
7 MUL1  
Affinity Capture-MS Homo sapiens
8 EFNA4  
Affinity Capture-MS Homo sapiens
9 ELMO3 79767
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
10 GRB2 2885
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Two-hybrid Homo sapiens
11 ELMO1 9844
Affinity Capture-MS Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
12 GAR1 54433
Affinity Capture-MS Homo sapiens
13 YWHAB 7529
Affinity Capture-MS Homo sapiens
14 PFN1 5216
Proximity Label-MS Homo sapiens
15 KRT8 3856
Proximity Label-MS Homo sapiens
16 NPTN 27020
Affinity Capture-MS Homo sapiens
17 RPL22L1 200916
Affinity Capture-MS Homo sapiens
18 FASLG 356
Protein-peptide Homo sapiens
19 ARHGAP11B  
Affinity Capture-MS Homo sapiens
20 VSIG8  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
21 EGFR 1956
Affinity Capture-MS Homo sapiens
22 EIF4A3 9775
Affinity Capture-MS Homo sapiens
23 ELMO2 63916
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
24 OCIAD1 54940
Affinity Capture-MS Homo sapiens
25 RNF115  
Two-hybrid Homo sapiens
26 ARHGEF1 9138
Affinity Capture-MS Homo sapiens
27 MAPRE3  
Proximity Label-MS Homo sapiens
28 RPS20 6224
Cross-Linking-MS (XL-MS) Homo sapiens
29 ITSN1 6453
Affinity Capture-MS Homo sapiens
30 STAT1 6772
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
31 HNRNPL 3191
Affinity Capture-MS Homo sapiens
32 ARHGAP36  
Affinity Capture-MS Homo sapiens
33 ARRB1 408
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
34 NTRK1 4914
Affinity Capture-MS Homo sapiens
35 PXN 5829
Proximity Label-MS Homo sapiens
36 HSPA1A 3303
Cross-Linking-MS (XL-MS) Homo sapiens
37 Abr 109934
Affinity Capture-MS Mus musculus
38 BICD2 23299
Proximity Label-MS Homo sapiens
39 BICD1 636
Proximity Label-MS Homo sapiens
40 MAGOH 4116
Affinity Capture-MS Homo sapiens
41 DOCK3  
Affinity Capture-MS Homo sapiens
42 DGCR2 9993
Affinity Capture-MS Homo sapiens
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