Gene description for SKIV2L
Gene name superkiller viralicidic activity 2-like (S. cerevisiae)
Gene symbol SKIV2L
Other names/aliases 170A
DDX13
HLP
SKI2
SKI2W
SKIV2
THES2
Species Homo sapiens
 Database cross references - SKIV2L
ExoCarta ExoCarta_6499
Vesiclepedia VP_6499
Entrez Gene 6499
HGNC 10898
MIM 600478
UniProt Q15477  
 SKIV2L identified in exosomes derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Chondrocytes 35931686    
Chondrocytes 35931686    
Mesenchymal stem cells 36408942    
Ovarian cancer cells 23333927    
Ovarian cancer cells 23333927    
Thymus 23844026    
 Gene ontology annotations for SKIV2L
Molecular Function
    DNA clamp loader activity GO:0003689 IEA
    RNA binding GO:0003723 IEA
    RNA helicase activity GO:0003724 IBA
    protein binding GO:0005515 IPI
    ATP binding GO:0005524 IEA
    ATP hydrolysis activity GO:0016887 IEA
    3'-5' RNA helicase activity GO:0034458 IDA
    cohesin loader activity GO:0061775 IEA
    chromatin extrusion motor activity GO:0140584 IEA
    ATP-dependent H3-H4 histone complex chaperone activity GO:0140665 IEA
    ATP-dependent H2AZ histone chaperone activity GO:0140849 IEA
Biological Process
    chromatin remodeling GO:0006338 IEA
    nuclear-transcribed mRNA catabolic process, 3'-5' exonucleolytic nonsense-mediated decay GO:0070478 IBA
    nuclear-transcribed mRNA catabolic process, 3'-5' exonucleolytic nonsense-mediated decay GO:0070478 IDA
    rescue of stalled ribosome GO:0072344 IDA
    chromatin looping GO:0140588 IEA
Subcellular Localization
    nucleus GO:0005634 IEA
    cytosol GO:0005829 TAS
    Ski complex GO:0055087 IBA
    Ski complex GO:0055087 IDA
 Experiment description of studies that identified SKIV2L in exosomes
1
Experiment ID 489
MISEV standards
EM
Biophysical techniques
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
Canx
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
EM
Biophysical techniques
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
Canx
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
EM
Biophysical techniques
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
Canx
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
EM
Biophysical techniques
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
Canx
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 497
MISEV standards
Biophysical techniques
CD9|CD151|CD63|CD81|CD82|FLOT1|FLOT2|GAPDH|LAMP1|LAMP2|RAB5A|SDCBP|TFRC|TSG101|UCHL1
Enriched markers
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35931686    
Organism Homo sapiens
Experiment description Extracellular vesicles enriched in connexin 43 promote a senescent phenotype in bone and synovial cells contributing to osteoarthritis progression
Authors "Varela-Eirín M, Carpintero-Fernández P, Guitián-Caamaño A, Varela-Vázquez A, García-Yuste A, Sánchez-Temprano A, Bravo-López SB, Yañez-Cabanas J, Fonseca E, Largo R, Mobasheri A, Caeiro JR, Mayán MD."
Journal name Cell Death Dis
Publication year 2022
Sample Chondrocytes
Sample name T/C-28a2
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
6
Experiment ID 498
MISEV standards
EM
Biophysical techniques
CD9|CD151|CD63|CD81|CD82|FLOT1|FLOT2|GAPDH|LAMP1|LAMP2|RAB5A|SDCBP|TFRC|TSG101|UCHL1
Enriched markers
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35931686    
Organism Homo sapiens
Experiment description Extracellular vesicles enriched in connexin 43 promote a senescent phenotype in bone and synovial cells contributing to osteoarthritis progression
Authors "Varela-Eirín M, Carpintero-Fernández P, Guitián-Caamaño A, Varela-Vázquez A, García-Yuste A, Sánchez-Temprano A, Bravo-López SB, Yañez-Cabanas J, Fonseca E, Largo R, Mobasheri A, Caeiro JR, Mayán MD."
Journal name Cell Death Dis
Publication year 2022
Sample Chondrocytes
Sample name T/C-28a2
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
7
Experiment ID 488
MISEV standards
EM
Biophysical techniques
CD9|CD81|CD63|GAPDH|SDCBP|LAMP1|TFRC|UCHL1|FLOT2|LAMP2|FLOT1|ICAM1|RAB5B|CD151|RAB35|TSG101|RAB5A|CD82
Enriched markers
CANX
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Homo sapiens
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Mesenchymal stem cells
Sample name UCMSC
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
8
Experiment ID 211
MISEV standards
EM
Biophysical techniques
TSG101|Alix|EpCAM|TFRC
Enriched markers
cytochrome c|GOLGA2
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors "Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K."
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name IGROV1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
9
Experiment ID 212
MISEV standards
CEM
Biophysical techniques
TSG101|Alix|EpCAM|TFRC
Enriched markers
Cytochrome C|GOLGA2
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors "Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K."
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name OVCAR-3
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
10
Experiment ID 217
MISEV standards
EM
Biophysical techniques
TSG101|CD81|CD9|CD63
Enriched markers
Negative markers
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23844026    
Organism Homo sapiens
Experiment description Characterization of human thymic exosomes.
Authors "Skogberg G, Gudmundsdottir J, van der Post S, Sandstrom K, Bruhn S, Benson M, Mincheva-Nilsson L, Baranov V, Telemo E, Ekwall O."
Journal name PLoS One
Publication year 2013
Sample Thymus
Sample name Normal-Thymus
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for SKIV2L
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 CBS 875
Co-fractionation Homo sapiens
2 UBE2H 7328
Affinity Capture-MS Homo sapiens
3 WDR1 9948
Two-hybrid Homo sapiens
4 FERMT1 55612
Co-fractionation Homo sapiens
5 EXOSC10 5394
Two-hybrid Homo sapiens
6 TNRC17  
Two-hybrid Homo sapiens
7 Eif3a 13669
Affinity Capture-MS Mus musculus
8 PLEKHA4 57664
Affinity Capture-MS Homo sapiens
9 RHOU 58480
Reconstituted Complex Homo sapiens
10 PFDN6 10471
Affinity Capture-MS Homo sapiens
11 LGR4 55366
Affinity Capture-MS Homo sapiens
12 ITSN1 6453
Co-fractionation Homo sapiens
13 SLFN11 91607
Affinity Capture-MS Homo sapiens
14 CDC73  
Affinity Capture-MS Homo sapiens
15 RBM8A 9939
Affinity Capture-MS Homo sapiens
16 KIF14 9928
Affinity Capture-MS Homo sapiens
17 EIF2S3 1968
Affinity Capture-MS Homo sapiens
18 C9orf78 51759
Affinity Capture-MS Homo sapiens
19 LIN28A  
Affinity Capture-MS Homo sapiens
20 CACNB3  
Two-hybrid Homo sapiens
21 MFSD4  
Affinity Capture-MS Homo sapiens
22 SIRT7  
Affinity Capture-MS Homo sapiens
23 ZNF746  
Affinity Capture-MS Homo sapiens
24 RPA2 6118
Proximity Label-MS Homo sapiens
25 TTC37 9652
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
26 PRKAA2 5563
Two-hybrid Homo sapiens
27 SRP9 6726
Affinity Capture-MS Homo sapiens
28 GPR182  
Affinity Capture-MS Homo sapiens
29 MGARP  
Affinity Capture-MS Homo sapiens
30 RPL10 6134
Affinity Capture-MS Homo sapiens
31 TRIM33 51592
Affinity Capture-MS Homo sapiens
32 EGFR 1956
Affinity Capture-MS Homo sapiens
33 SRP72 6731
Affinity Capture-MS Homo sapiens
34 CDKL5  
Two-hybrid Homo sapiens
35 CAPRIN1 4076
Affinity Capture-MS Homo sapiens
36 HSD17B13  
Affinity Capture-MS Homo sapiens
37 PMPCA 23203
Two-hybrid Homo sapiens
38 PFDN4 5203
Affinity Capture-MS Homo sapiens
39 FKBP5 2289
Affinity Capture-MS Homo sapiens
40 XRN1 54464
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
41 CCT7 10574
Affinity Capture-MS Homo sapiens
42 VBP1 7411
Affinity Capture-MS Homo sapiens
43 KIAA1429 25962
Affinity Capture-MS Homo sapiens
44 FBXW4 6468
Affinity Capture-MS Homo sapiens
45 WDR61 80349
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
46 BSG 682
Affinity Capture-MS Homo sapiens
47 PFDN2 5202
Affinity Capture-MS Homo sapiens
48 ALYREF 10189
Co-fractionation Homo sapiens
49 PSPC1 55269
Affinity Capture-MS Homo sapiens
50 RPA3 6119
Proximity Label-MS Homo sapiens
51 NDOR1  
Co-fractionation Homo sapiens
52 RAN 5901
Co-fractionation Homo sapiens
53 TTC33  
Affinity Capture-MS Homo sapiens
54 NTRK1 4914
Affinity Capture-MS Homo sapiens
55 GPR45  
Affinity Capture-MS Homo sapiens
56 SRSF7 6432
Affinity Capture-MS Homo sapiens
57 SPRTN  
Affinity Capture-MS Homo sapiens
58 HIST1H1E 3008
Affinity Capture-MS Homo sapiens
59 Rrbp1  
Affinity Capture-MS Mus musculus
60 PHF5A 84844
Affinity Capture-MS Homo sapiens
61 FAM174A 345757
Affinity Capture-MS Homo sapiens
62 BRD1 23774
Affinity Capture-MS Homo sapiens
63 HIST1H1C 3006
Affinity Capture-MS Homo sapiens
64 TRIM66  
Affinity Capture-MS Homo sapiens
65 NPM1 4869
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
66 C9orf72  
Affinity Capture-MS Homo sapiens
67 Rpl35 66489
Affinity Capture-MS Mus musculus
68 PAF1 54623
Affinity Capture-MS Homo sapiens
69 RAB4B 53916
Affinity Capture-MS Homo sapiens
70 FSCN1 6624
Affinity Capture-MS Homo sapiens
71 PLCG1 5335
Co-fractionation Homo sapiens
72 TSSK2  
Two-hybrid Homo sapiens
73 GALNT13 114805
Two-hybrid Homo sapiens
74 PFDN1 5201
Affinity Capture-MS Homo sapiens
75 PFDN5 5204
Affinity Capture-MS Homo sapiens
76 CDK11A 728642
Two-hybrid Homo sapiens
77 EXOSC4 54512
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
78 SNRPB 6628
Two-hybrid Homo sapiens
79 UBXN6 80700
Affinity Capture-MS Homo sapiens
80 RPS16 6217
Affinity Capture-MS Homo sapiens
View the network image/svg+xml



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