Gene description for Eif3a
Gene name eukaryotic translation initiation factor 3, subunit A
Gene symbol Eif3a
Other names/aliases A830012B05Rik
Csma
Eif3
Eif3s10
mKIAA0139
Species Mus musculus
 Database cross references - Eif3a
ExoCarta ExoCarta_13669
Vesiclepedia VP_13669
Entrez Gene 13669
UniProt P23116  
 Eif3a identified in exosomes derived from the following tissue/cell type
Colon cancer cells 37309723    
Fibroblasts 23260141    
Macrophages 23658846    
Macrophages 23658846    
Macrophages 23658846    
Mast cells 17486113    
Pancreatic cells 19351151    
T-cell lymphoma cells 37309723    
 Gene ontology annotations for Eif3a
Molecular Function
    RNA binding GO:0003723 IEA
    RNA binding GO:0003723 ISO
    mRNA binding GO:0003729 IBA
    translation initiation factor activity GO:0003743 IBA
    translation initiation factor activity GO:0003743 IDA
    translation initiation factor activity GO:0003743 ISO
    protein binding GO:0005515 IPI
    receptor tyrosine kinase binding GO:0030971 IEA
    receptor tyrosine kinase binding GO:0030971 ISO
Biological Process
    formation of cytoplasmic translation initiation complex GO:0001732 IBA
    formation of cytoplasmic translation initiation complex GO:0001732 IDA
    formation of cytoplasmic translation initiation complex GO:0001732 ISO
    translation reinitiation GO:0002188 IBA
    negative regulation of ERK1 and ERK2 cascade GO:0070373 IEA
    negative regulation of ERK1 and ERK2 cascade GO:0070373 ISO
    IRES-dependent viral translational initiation GO:0075522 IEA
    IRES-dependent viral translational initiation GO:0075522 ISO
    viral translational termination-reinitiation GO:0075525 IEA
    viral translational termination-reinitiation GO:0075525 ISO
Subcellular Localization
    nucleoplasm GO:0005654 IEA
    nucleoplasm GO:0005654 ISO
    nucleolus GO:0005730 IEA
    nucleolus GO:0005730 ISO
    centrosome GO:0005813 IEA
    cytosol GO:0005829 IEA
    cytosol GO:0005829 ISO
    eukaryotic translation initiation factor 3 complex GO:0005852 IDA
    eukaryotic translation initiation factor 3 complex GO:0005852 ISO
    microtubule GO:0005874 IEA
    microtubule GO:0005874 ISO
    postsynaptic density GO:0014069 EXP
    postsynaptic density GO:0014069 IDA
    eukaryotic 43S preinitiation complex GO:0016282 IEA
    eukaryotic 48S preinitiation complex GO:0033290 IEA
    multi-eIF complex GO:0043614 IBA
    eukaryotic translation initiation factor 3 complex, eIF3e GO:0071540 IBA
    eukaryotic translation initiation factor 3 complex, eIF3m GO:0071541 IBA
    eukaryotic translation initiation factor 3 complex, eIF3m GO:0071541 IDA
 Experiment description of studies that identified Eif3a in exosomes
1
Experiment ID 907
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample Colon cancer cells
Sample name C26
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 210
MISEV standards
✔
EM
Biophysical techniques
✔
CD81|FLOT1
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23260141    
Organism Mus musculus
Experiment description Exosomes Mediate Stromal Mobilization of Autocrine Wnt-PCP Signaling in Breast Cancer Cell Migration.
Authors "Luga V, Zhang L, Viloria-Petit AM, Ogunjimi AA, Inanlou MR, Chiu E, Buchanan M, Hosein AN, Basik M, Wrana JL."
Journal name Cell
Publication year 2012
Sample Fibroblasts
Sample name Normal-Fibroblasts (L cells)
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
3
Experiment ID 214
MISEV standards
✔
EM
Biophysical techniques
✘ Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23658846    
Organism Mus musculus
Experiment description Immunomodulatory impact of leishmania-induced macrophage exosomes: a comparative proteomic and functional analysis.
Authors "Hassani K, Olivier M."
Journal name PLoS Negl Trop Dis
Publication year 2013
Sample Macrophages
Sample name Leishmania-infected-Macrophage (J774A.1)
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Filtration
Protease inhibitors
Sucrose density gradient
Filtration
Ultracentrifugation
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
4
Experiment ID 215
MISEV standards
✔
EM
Biophysical techniques
✘ Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23658846    
Organism Mus musculus
Experiment description Immunomodulatory impact of leishmania-induced macrophage exosomes: a comparative proteomic and functional analysis.
Authors "Hassani K, Olivier M."
Journal name PLoS Negl Trop Dis
Publication year 2013
Sample Macrophages
Sample name LPS-treated-Macrophage (J774A.1)
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Filtration
Protease inhibitors
Sucrose density gradient
Filtration
Ultracentrifugation
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
5
Experiment ID 216
MISEV standards
✔
EM
Biophysical techniques
✘ Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23658846    
Organism Mus musculus
Experiment description Immunomodulatory impact of leishmania-induced macrophage exosomes: a comparative proteomic and functional analysis.
Authors "Hassani K, Olivier M."
Journal name PLoS Negl Trop Dis
Publication year 2013
Sample Macrophages
Sample name Normal-Macrophage (J774A.1)
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Filtration
Protease inhibitors
Sucrose density gradient
Filtration
Ultracentrifugation
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
6
Experiment ID 15
MISEV standards
✔
EM
Biophysical techniques
✔
CD63
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 17486113    
Organism Mus musculus
Homo sapiens
Experiment description Exosome-mediated transfer of mRNAs and microRNAs is a novel mechanism of genetic exchange between cells.
Authors "Valadi H, Ekstrom K, Bossios A, Sjostrand M, Lee JJ, Lotvall JO"
Journal name NCB
Publication year 2007
Sample Mast cells
Sample name MC9
Bone marrow-derived mast cells
HMC-1
Isolation/purification methods Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.11-1.21 g/mL
Molecules identified in the study Protein
mRNA
miRNA
Methods used in the study Mass spectrometry [MALDI TOF]
Western blotting
Microarray
miRCURY LNA Array
7
Experiment ID 188
MISEV standards
✔
EM
Biophysical techniques
✔
GAPDH|UCHL1|HSP90
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 19351151    
Organism Mus musculus
Experiment description Characterization of vesicles secreted from insulinoma NIT-1 cells.
Authors "Lee HS, Jeong J, Lee KJ."
Journal name J Proteome Res
Publication year 2009
Sample Pancreatic cells
Sample name Pancreatic beta cell (NIT-1)
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
8
Experiment ID 908
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample T-cell lymphoma cells
Sample name EL4
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for Eif3a
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 GNL2 29889
Affinity Capture-MS Homo sapiens
2 RPSA 3921
Affinity Capture-MS Homo sapiens
3 EIF3A 8661
Affinity Capture-MS Homo sapiens
4 Kctd13  
Affinity Capture-MS Mus musculus
5 MPG 4350
Affinity Capture-MS Homo sapiens
6 MRPL48  
Affinity Capture-MS Homo sapiens
7 FAM98A 25940
Affinity Capture-MS Homo sapiens
8 RPS3A 6189
Affinity Capture-MS Homo sapiens
9 SKIV2L 6499
Affinity Capture-MS Homo sapiens
10 RPL13 6137
Affinity Capture-MS Homo sapiens
11 EIF3K 27335
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
12 RPL13A 23521
Affinity Capture-MS Homo sapiens
13 G3BP1 10146
Affinity Capture-MS Homo sapiens
14 RPS19 6223
Affinity Capture-MS Homo sapiens
15 RPL10 6134
Affinity Capture-MS Homo sapiens
16 RPL14 9045
Affinity Capture-MS Homo sapiens
17 EIF4A1 1973
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
18 FAU 2197
Affinity Capture-MS Homo sapiens
19 RPS3 6188
Affinity Capture-MS Homo sapiens
20 RPS18 6222
Affinity Capture-MS Homo sapiens
21 PSIP1 11168
Affinity Capture-MS Homo sapiens
22 RPS20 6224
Affinity Capture-MS Homo sapiens
23 Rps9 76846
Co-fractionation Mus musculus
24 Unk  
Affinity Capture-RNA Mus musculus
25 CCDC137  
Affinity Capture-MS Homo sapiens
26 EIF3B 8662
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
27 RPL24 6152
Affinity Capture-MS Homo sapiens
28 NPM1 4869
Affinity Capture-MS Homo sapiens
29 Paip1  
Affinity Capture-Western Mus musculus
30 Atg16l1  
Affinity Capture-MS Mus musculus
31 EIF3E 3646
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
32 NMT1 4836
Affinity Capture-MS Homo sapiens
33 RPS11 6205
Affinity Capture-MS Homo sapiens
34 ERI1  
Affinity Capture-MS Homo sapiens
35 RPL19 6143
Affinity Capture-MS Homo sapiens
36 TRMT10C 54931
Affinity Capture-MS Homo sapiens
37 LARP4B 23185
Affinity Capture-MS Homo sapiens
38 EIF3C 8663
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
39 BRIX1 55299
Affinity Capture-MS Homo sapiens
40 TTC37 9652
Affinity Capture-MS Homo sapiens
41 RPL27 6155
Affinity Capture-MS Homo sapiens
42 NSUN2 54888
Affinity Capture-MS Homo sapiens
43 SRP14 6727
Affinity Capture-MS Homo sapiens
44 RPL4 6124
Affinity Capture-MS Homo sapiens
45 Ddb1 13194
Affinity Capture-MS Mus musculus
46 RPS10 6204
Affinity Capture-MS Homo sapiens
47 RPL5 6125
Affinity Capture-MS Homo sapiens
48 RPL18A 6142
Affinity Capture-MS Homo sapiens
49 RPL27A 6157
Affinity Capture-MS Homo sapiens
50 RFC4 5984
Affinity Capture-MS Homo sapiens
51 DDX1 1653
Affinity Capture-MS Homo sapiens
52 EIF4G2 1982
Affinity Capture-MS Homo sapiens
53 EIF3H 8667
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
54 SND1 27044
Affinity Capture-MS Homo sapiens
55 RPS15A 6210
Affinity Capture-MS Homo sapiens
56 GTPBP4 23560
Affinity Capture-MS Homo sapiens
57 EIF5B 9669
Affinity Capture-MS Homo sapiens
58 EIF4A2 1974
Affinity Capture-MS Homo sapiens
59 LARP1 23367
Affinity Capture-MS Homo sapiens
60 Rps18 20084
Co-fractionation Mus musculus
61 EIF3G 8666
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
62 RPL32 6161
Affinity Capture-MS Homo sapiens
63 RPL10A 4736
Affinity Capture-MS Homo sapiens
64 LARP4 113251
Affinity Capture-MS Homo sapiens
65 RPS2 6187
Affinity Capture-MS Homo sapiens
66 BAZ1B 9031
Affinity Capture-MS Homo sapiens
67 EIF4G1 1981
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
68 ASCC1 51008
Affinity Capture-MS Homo sapiens
69 ZNF622 90441
Affinity Capture-MS Homo sapiens
70 NAT10 55226
Affinity Capture-MS Homo sapiens
71 DHX29 54505
Affinity Capture-MS Homo sapiens
72 NPM3 10360
Affinity Capture-MS Homo sapiens
73 RPL3 6122
Affinity Capture-MS Homo sapiens
74 HSD17B10 3028
Affinity Capture-MS Homo sapiens
75 EIF4E 1977
Affinity Capture-MS Homo sapiens
76 Eif1 20918
Co-fractionation Mus musculus
77 RPL7 6129
Affinity Capture-MS Homo sapiens
78 POLR2A 5430
Affinity Capture-MS Homo sapiens
79 RPS27 6232
Affinity Capture-MS Homo sapiens
80 HIST1H1B 3009
Affinity Capture-MS Homo sapiens
81 Rps10 67097
Co-fractionation Mus musculus
82 DDX47 51202
Affinity Capture-MS Homo sapiens
83 Uba5  
Co-fractionation Mus musculus
84 LYAR 55646
Affinity Capture-MS Homo sapiens
85 RPS14 6208
Affinity Capture-MS Homo sapiens
86 RPL35A 6165
Affinity Capture-MS Homo sapiens
87 RPL22 6146
Affinity Capture-MS Homo sapiens
88 HP1BP3 50809
Affinity Capture-MS Homo sapiens
89 PPAN 56342
Affinity Capture-MS Homo sapiens
90 Eif3h 68135
Co-fractionation Mus musculus
91 Eed  
Affinity Capture-MS Mus musculus
92 RPL11 6135
Affinity Capture-MS Homo sapiens
93 Eif3e 16341
Co-fractionation Mus musculus
94 TSR1 55720
Affinity Capture-MS Homo sapiens
95 GNB2L1 10399
Affinity Capture-MS Homo sapiens
96 Rps20 67427
Co-fractionation Mus musculus
97 EIF3D 8664
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
98 RPS16 6217
Affinity Capture-MS Homo sapiens
99 Eif5b 226982
Co-fractionation Mus musculus
100 PA2G4 5036
Affinity Capture-MS Homo sapiens
101 SUPT16H 11198
Affinity Capture-MS Homo sapiens
102 Foxl2  
Two-hybrid Mus musculus
103 EIF3F 8665
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
104 Rps25 75617
Co-fractionation Mus musculus
105 GIGYF2 26058
Affinity Capture-MS Homo sapiens
106 PRRC2A 7916
Affinity Capture-MS Homo sapiens
107 RPS25 6230
Affinity Capture-MS Homo sapiens
108 RPS21 6227
Affinity Capture-MS Homo sapiens
109 EIF3I 8668
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
110 C9orf114  
Affinity Capture-MS Homo sapiens
111 EIF1AX 1964
Affinity Capture-MS Homo sapiens
112 DDX3X 1654
Affinity Capture-MS Homo sapiens
113 PRRC2C 23215
Affinity Capture-MS Homo sapiens
114 Atxn1  
Affinity Capture-MS Mus musculus
115 RPS12 6206
Affinity Capture-MS Homo sapiens
116 RPS15 6209
Affinity Capture-MS Homo sapiens
117 SRP72 6731
Affinity Capture-MS Homo sapiens
118 Chaf1a  
Affinity Capture-MS Mus musculus
119 TRMT112 51504
Affinity Capture-MS Homo sapiens
120 Eif3k 73830
Co-fractionation Mus musculus
121 PRRC2B  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
122 Mettl21e  
Affinity Capture-MS Mus musculus
123 Fzr1  
Affinity Capture-MS Mus musculus
124 RPS28 6234
Affinity Capture-MS Homo sapiens
125 ERCC6  
Affinity Capture-MS Homo sapiens
126 RPL38 6169
Affinity Capture-MS Homo sapiens
127 RPS24 6229
Affinity Capture-MS Homo sapiens
128 Cdkn2a  
Affinity Capture-Western Mus musculus
Two-hybrid Mus musculus
129 EIF3J 8669
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
130 RTCB 51493
Affinity Capture-MS Homo sapiens
131 RPL7A 6130
Affinity Capture-MS Homo sapiens
132 Eif3m 98221
Co-fractionation Mus musculus
133 C14orf169  
Affinity Capture-MS Homo sapiens
134 RPS6 6194
Affinity Capture-MS Homo sapiens
135 RPL8 6132
Affinity Capture-MS Homo sapiens
136 SERBP1 26135
Affinity Capture-MS Homo sapiens
137 Rps26 27370
Co-fractionation Mus musculus
138 RPL23A 6147
Affinity Capture-MS Homo sapiens
139 RPS29 6235
Affinity Capture-MS Homo sapiens
140 HNRNPU 3192
Affinity Capture-MS Homo sapiens
141 EIF3M 10480
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
142 DDX31  
Affinity Capture-MS Homo sapiens
143 EMG1 10436
Affinity Capture-MS Homo sapiens
144 RPL17 6139
Affinity Capture-MS Homo sapiens
145 EIF3L 51386
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
View the network image/svg+xml



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