Gene description for Eif3m
Gene name eukaryotic translation initiation factor 3, subunit M
Gene symbol Eif3m
Other names/aliases Ga17
Pcid1
Tango7
Species Mus musculus
 Database cross references - Eif3m
ExoCarta ExoCarta_98221
Vesiclepedia VP_98221
Entrez Gene 98221
UniProt Q99JX4  
 Eif3m identified in sEVs derived from the following tissue/cell type
Colon cancer cells 37309723    
Neural stem cells 25242146    
T-cell lymphoma cells 37309723    
 Gene ontology annotations for Eif3m
Molecular Function
    translation initiation factor activity GO:0003743 IEA
    translation initiation factor activity GO:0003743 ISO
    translation initiation factor binding GO:0031369 IDA
Biological Process
    formation of cytoplasmic translation initiation complex GO:0001732 IEA
    cytoplasmic translational initiation GO:0002183 IBA
    translation GO:0006412 IMP
Subcellular Localization
    eukaryotic translation initiation factor 3 complex GO:0005852 IBA
    eukaryotic translation initiation factor 3 complex GO:0005852 ISO
    eukaryotic 43S preinitiation complex GO:0016282 IEA
    eukaryotic 48S preinitiation complex GO:0033290 IEA
    eukaryotic translation initiation factor 3 complex, eIF3m GO:0071541 IDA
 Experiment description of studies that identified Eif3m in sEVs
1
Experiment ID 907
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample Colon cancer cells
Sample name C26
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 263
MISEV standards
✘ Biophysical techniques
✔
CD63|CD9
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25242146    
Organism Mus musculus
Experiment description Extracellular Vesicles from Neural Stem Cells Transfer IFN-? via Ifngr1 to Activate Stat1 Signaling in Target Cells
Authors "Chiara Cossetti, Nunzio Iraci, Tim R. Mercer, Tommaso Leonardi, Emanuele Alpi, Denise Drago, Clara Alfaro-Cervello, Harpreet K. Saini, Matthew P. Davis, Julia Schaeffer, Beatriz Vega, Matilde Stefanini, CongJian Zhao, Werner Muller, Jose Manuel Garcia-Verdugo, Suresh Mathivanan, Angela Bachi, Anton J. Enright, John S. Mattick, Stefano Pluchino"
Journal name Molecular Cell
Publication year 2014
Sample Neural stem cells
Sample name NPCs - Th1 treated
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.20 g/mL
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
3
Experiment ID 908
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample T-cell lymphoma cells
Sample name EL4
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for Eif3m
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Mettl21e  
Affinity Capture-MS Mus musculus
2 Tmem173  
Proximity Label-MS Mus musculus
3 Fzr1  
Affinity Capture-MS Mus musculus
4 Eif3a 13669
Co-fractionation Mus musculus
5 Eed  
Affinity Capture-MS Mus musculus
6 Fitm2  
Positive Genetic Mus musculus
7 Chaf1a  
Affinity Capture-MS Mus musculus
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