Gene description for Eif3e
Gene name eukaryotic translation initiation factor 3, subunit E
Gene symbol Eif3e
Other names/aliases 48kDa
Eif3s6
Int6
eIF3-p46
eIF3-p48
Species Mus musculus
 Database cross references - Eif3e
ExoCarta ExoCarta_16341
Vesiclepedia VP_16341
Entrez Gene 16341
UniProt P60229  
 Eif3e identified in exosomes derived from the following tissue/cell type
Colon cancer cells 37309723    
Fibroblasts 23260141    
Mast cells 17486113    
Neural stem cells 25242146    
T-cell lymphoma cells 37309723    
 Gene ontology annotations for Eif3e
Molecular Function
    translation initiation factor activity GO:0003743 IDA
    translation initiation factor activity GO:0003743 ISO
    transmembrane transporter binding GO:0044325 ISO
Biological Process
    nuclear-transcribed mRNA catabolic process, nonsense-mediated decay GO:0000184 ISO
    nuclear-transcribed mRNA catabolic process, nonsense-mediated decay GO:0000184 ISS
    formation of cytoplasmic translation initiation complex GO:0001732 IEA
    translational initiation GO:0006413 IBA
    translational initiation GO:0006413 IDA
    translational initiation GO:0006413 ISO
    intracellular calcium ion homeostasis GO:0006874 ISO
    positive regulation of translation GO:0045727 IEA
    positive regulation of translation GO:0045727 ISO
    positive regulation of intracellular protein transport GO:0090316 ISO
    positive regulation of mRNA binding GO:1902416 ISO
Subcellular Localization
    nucleus GO:0005634 IBA
    nucleus GO:0005634 ISO
    cytoplasm GO:0005737 ISO
    cytosol GO:0005829 IEA
    cytosol GO:0005829 ISO
    eukaryotic translation initiation factor 3 complex GO:0005852 IBA
    eukaryotic translation initiation factor 3 complex GO:0005852 IDA
    eukaryotic translation initiation factor 3 complex GO:0005852 ISO
    postsynaptic density GO:0014069 EXP
    postsynaptic density GO:0014069 IDA
    eukaryotic 43S preinitiation complex GO:0016282 IEA
    PML body GO:0016605 IEA
    PML body GO:0016605 ISO
    eukaryotic 48S preinitiation complex GO:0033290 IEA
    eukaryotic translation initiation factor 3 complex, eIF3e GO:0071540 IEA
 Experiment description of studies that identified Eif3e in exosomes
1
Experiment ID 907
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample Colon cancer cells
Sample name C26
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 210
MISEV standards
✔
EM
Biophysical techniques
✔
CD81|FLOT1
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23260141    
Organism Mus musculus
Experiment description Exosomes Mediate Stromal Mobilization of Autocrine Wnt-PCP Signaling in Breast Cancer Cell Migration.
Authors "Luga V, Zhang L, Viloria-Petit AM, Ogunjimi AA, Inanlou MR, Chiu E, Buchanan M, Hosein AN, Basik M, Wrana JL."
Journal name Cell
Publication year 2012
Sample Fibroblasts
Sample name Normal-Fibroblasts (L cells)
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
3
Experiment ID 15
MISEV standards
✔
EM
Biophysical techniques
✔
CD63
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule mrna
Identification method Microarray
PubMed ID 17486113    
Organism Mus musculus
Homo sapiens
Experiment description Exosome-mediated transfer of mRNAs and microRNAs is a novel mechanism of genetic exchange between cells.
Authors "Valadi H, Ekstrom K, Bossios A, Sjostrand M, Lee JJ, Lotvall JO"
Journal name NCB
Publication year 2007
Sample Mast cells
Sample name MC9
Bone marrow-derived mast cells
HMC-1
Isolation/purification methods Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.11-1.21 g/mL
Molecules identified in the study Protein
mRNA
miRNA
Methods used in the study Mass spectrometry [MALDI TOF]
Western blotting
Microarray
miRCURY LNA Array
4
Experiment ID 263
MISEV standards
✘ Biophysical techniques
✔
CD63|CD9
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25242146    
Organism Mus musculus
Experiment description Extracellular Vesicles from Neural Stem Cells Transfer IFN-? via Ifngr1 to Activate Stat1 Signaling in Target Cells
Authors "Chiara Cossetti, Nunzio Iraci, Tim R. Mercer, Tommaso Leonardi, Emanuele Alpi, Denise Drago, Clara Alfaro-Cervello, Harpreet K. Saini, Matthew P. Davis, Julia Schaeffer, Beatriz Vega, Matilde Stefanini, CongJian Zhao, Werner Muller, Jose Manuel Garcia-Verdugo, Suresh Mathivanan, Angela Bachi, Anton J. Enright, John S. Mattick, Stefano Pluchino"
Journal name Molecular Cell
Publication year 2014
Sample Neural stem cells
Sample name NPCs - Th1 treated
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.20 g/mL
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
5
Experiment ID 908
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample T-cell lymphoma cells
Sample name EL4
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for Eif3e
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 RPSA 3921
Affinity Capture-MS Homo sapiens
2 EIF3A 8661
Affinity Capture-MS Homo sapiens
3 UTP18  
Affinity Capture-MS Homo sapiens
4 IGF2BP1 10642
Affinity Capture-MS Homo sapiens
5 RPS3A 6189
Affinity Capture-MS Homo sapiens
6 EIF3C 8663
Affinity Capture-MS Homo sapiens
7 GOLPH3 64083
Affinity Capture-MS Homo sapiens
8 EIF3K 27335
Affinity Capture-MS Homo sapiens
9 TOP2B 7155
Affinity Capture-MS Homo sapiens
10 RPL13A 23521
Affinity Capture-MS Homo sapiens
11 RPL37A 6168
Affinity Capture-MS Homo sapiens
12 TBC1D16  
Affinity Capture-MS Homo sapiens
13 RPS19 6223
Affinity Capture-MS Homo sapiens
14 RPL10 6134
Affinity Capture-MS Homo sapiens
15 RPL15 6138
Affinity Capture-MS Homo sapiens
16 GTPBP1 9567
Affinity Capture-MS Homo sapiens
17 EIF4A1 1973
Affinity Capture-MS Homo sapiens
18 BRWD1  
Affinity Capture-MS Homo sapiens
19 FAU 2197
Affinity Capture-MS Homo sapiens
20 RPS3 6188
Affinity Capture-MS Homo sapiens
21 Tmem173  
Proximity Label-MS Mus musculus
22 RPS18 6222
Affinity Capture-MS Homo sapiens
23 PSIP1 11168
Affinity Capture-MS Homo sapiens
24 DIMT1 27292
Affinity Capture-MS Homo sapiens
25 TOP2A 7153
Affinity Capture-MS Homo sapiens
26 RPS20 6224
Affinity Capture-MS Homo sapiens
27 Unk  
Affinity Capture-RNA Mus musculus
28 EIF3B 8662
Affinity Capture-MS Homo sapiens
29 NPM1 4869
Affinity Capture-MS Homo sapiens
30 RPS8 6202
Affinity Capture-MS Homo sapiens
31 LSG1  
Affinity Capture-MS Homo sapiens
32 Atg16l1  
Affinity Capture-MS Mus musculus
33 POLRMT 5442
Affinity Capture-MS Homo sapiens
34 Eif3a 13669
Co-fractionation Mus musculus
35 C12orf43  
Affinity Capture-MS Homo sapiens
36 RPS11 6205
Affinity Capture-MS Homo sapiens
37 ERI1  
Affinity Capture-MS Homo sapiens
38 RPL19 6143
Affinity Capture-MS Homo sapiens
39 BRIX1 55299
Affinity Capture-MS Homo sapiens
40 RPL27 6155
Affinity Capture-MS Homo sapiens
41 PNO1 56902
Affinity Capture-MS Homo sapiens
42 RPL4 6124
Affinity Capture-MS Homo sapiens
43 RPS10 6204
Affinity Capture-MS Homo sapiens
44 BLM 641
Affinity Capture-MS Homo sapiens
45 NOP2 4839
Affinity Capture-MS Homo sapiens
46 RPL5 6125
Affinity Capture-MS Homo sapiens
47 RPL18A 6142
Affinity Capture-MS Homo sapiens
48 RPL27A 6157
Affinity Capture-MS Homo sapiens
49 EIF4G2 1982
Affinity Capture-MS Homo sapiens
50 RPLP0 6175
Affinity Capture-MS Homo sapiens
51 RPS16 6217
Affinity Capture-MS Homo sapiens
52 RPS15A 6210
Affinity Capture-MS Homo sapiens
53 GTPBP4 23560
Affinity Capture-MS Homo sapiens
54 SRPK1 6732
Affinity Capture-MS Homo sapiens
55 RPL11 6135
Affinity Capture-MS Homo sapiens
56 CGGBP1 8545
Affinity Capture-MS Homo sapiens
57 EIF3G 8666
Affinity Capture-MS Homo sapiens
58 RPL32 6161
Affinity Capture-MS Homo sapiens
59 SPATS2L 26010
Affinity Capture-MS Homo sapiens
60 RPL23 9349
Affinity Capture-MS Homo sapiens
61 RPS2 6187
Affinity Capture-MS Homo sapiens
62 PLA2G4A 5321
Affinity Capture-MS Homo sapiens
63 EIF4G1 1981
Affinity Capture-MS Homo sapiens
64 SMARCA5 8467
Affinity Capture-MS Homo sapiens
65 ZNF622 90441
Affinity Capture-MS Homo sapiens
66 NAT10 55226
Affinity Capture-MS Homo sapiens
67 RFC2 5982
Affinity Capture-MS Homo sapiens
68 Rps19 20085
Co-fractionation Mus musculus
69 PHIP 55023
Affinity Capture-MS Homo sapiens
70 RPS7 6201
Affinity Capture-MS Homo sapiens
71 RPL21 6144
Affinity Capture-MS Homo sapiens
72 RPL3 6122
Affinity Capture-MS Homo sapiens
73 EBNA1BP2 10969
Affinity Capture-MS Homo sapiens
74 RPS27 6232
Affinity Capture-MS Homo sapiens
75 RPL17 6139
Affinity Capture-MS Homo sapiens
76 RPS13 6207
Affinity Capture-MS Homo sapiens
77 CSNK2A2 1459
Affinity Capture-MS Homo sapiens
78 RPS14 6208
Affinity Capture-MS Homo sapiens
79 RPL12 6136
Affinity Capture-MS Homo sapiens
80 RPL22 6146
Affinity Capture-MS Homo sapiens
81 HP1BP3 50809
Affinity Capture-MS Homo sapiens
82 PPP6C 5537
Affinity Capture-MS Homo sapiens
83 PPAN 56342
Affinity Capture-MS Homo sapiens
84 Eed  
Affinity Capture-MS Mus musculus
85 SUPT16H 11198
Affinity Capture-MS Homo sapiens
86 GNL3 26354
Affinity Capture-MS Homo sapiens
87 Fancd2  
Affinity Capture-MS Mus musculus
88 EIF3D 8664
Affinity Capture-MS Homo sapiens
89 EIF3H 8667
Affinity Capture-MS Homo sapiens
90 RPL13 6137
Affinity Capture-MS Homo sapiens
91 EIF3F 8665
Affinity Capture-MS Homo sapiens
92 ILF3 3609
Affinity Capture-MS Homo sapiens
93 FBXL6  
Affinity Capture-MS Homo sapiens
94 RPL6 6128
Affinity Capture-MS Homo sapiens
95 RPS21 6227
Affinity Capture-MS Homo sapiens
96 EIF3I 8668
Affinity Capture-MS Homo sapiens
97 MINA 84864
Affinity Capture-MS Homo sapiens
98 Atxn1  
Proximity Label-MS Mus musculus
Affinity Capture-MS Mus musculus
99 RPS12 6206
Affinity Capture-MS Homo sapiens
100 RPS4X 6191
Affinity Capture-MS Homo sapiens
101 RPS15 6209
Affinity Capture-MS Homo sapiens
102 Chaf1a  
Affinity Capture-MS Mus musculus
103 TRMT112 51504
Affinity Capture-MS Homo sapiens
104 PRRC2B  
Affinity Capture-MS Homo sapiens
105 Mettl21e  
Affinity Capture-MS Mus musculus
106 Dcc  
Affinity Capture-Western Mus musculus
107 RPL10A 4736
Affinity Capture-MS Homo sapiens
108 RPL38 6169
Affinity Capture-MS Homo sapiens
109 RPS24 6229
Affinity Capture-MS Homo sapiens
110 EIF3J 8669
Affinity Capture-MS Homo sapiens
111 IBTK 25998
Affinity Capture-MS Homo sapiens
112 RPL7A 6130
Affinity Capture-MS Homo sapiens
113 RPS6 6194
Affinity Capture-MS Homo sapiens
114 RPL8 6132
Affinity Capture-MS Homo sapiens
115 RPL23A 6147
Affinity Capture-MS Homo sapiens
116 RPS29 6235
Affinity Capture-MS Homo sapiens
117 HNRNPU 3192
Affinity Capture-MS Homo sapiens
118 EIF3M 10480
Affinity Capture-MS Homo sapiens
119 CUL4A 8451
Affinity Capture-MS Homo sapiens
120 EIF3L 51386
Affinity Capture-MS Homo sapiens
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