Gene description for GTPBP1
Gene name GTP binding protein 1
Gene symbol GTPBP1
Other names/aliases GP-1
GP1
HSPC018
Species Homo sapiens
 Database cross references - GTPBP1
ExoCarta ExoCarta_9567
Vesiclepedia VP_9567
Entrez Gene 9567
HGNC 4669
MIM 602245
UniProt O00178  
 GTPBP1 identified in exosomes derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Colorectal cancer cells 23161513    
Colorectal cancer cells 23161513    
Colorectal cancer cells 23161513    
Mesenchymal stem cells 36408942    
Thymus 23844026    
 Gene ontology annotations for GTPBP1
Molecular Function
    tRNA binding GO:0000049 IDA
    RNA binding GO:0003723 HDA
    translation elongation factor activity GO:0003746 IBA
    translation elongation factor activity GO:0003746 IDA
    GTPase activity GO:0003924 IDA
    GTPase activity GO:0003924 ISS
    protein binding GO:0005515 IPI
    GTP binding GO:0005525 IEA
    alpha-aminoacyl-tRNA binding GO:1904678 IDA
Biological Process
    cytoplasmic translation GO:0002181 IDA
    translational elongation GO:0006414 IBA
    immune response GO:0006955 TAS
    signal transduction GO:0007165 TAS
    GTP metabolic process GO:0046039 ISS
    positive regulation of mRNA catabolic process GO:0061014 ISS
Subcellular Localization
    cytoplasmic exosome (RNase complex) GO:0000177 ISS
    cytosol GO:0005829 ISS
    membrane GO:0016020 HDA
 Experiment description of studies that identified GTPBP1 in exosomes
1
Experiment ID 489
MISEV standards
EM
Biophysical techniques
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
Canx
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
EM
Biophysical techniques
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
Canx
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
EM
Biophysical techniques
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
Canx
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
EM
Biophysical techniques
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
Canx
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 207
MISEV standards
EM
Biophysical techniques
TSG101|HSP70|FLOT1
Enriched markers
VDAC
Negative markers
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23161513    
Organism Homo sapiens
Experiment description Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS.
Authors "Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ."
Journal name Mol Cell Proteomics
Publication year 2012
Sample Colorectal cancer cells
Sample name DKO-1
Isolation/purification methods Differential centrifugation
Filtration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
6
Experiment ID 208
MISEV standards
EM
Biophysical techniques
TSG101|HSP70|FLOT1
Enriched markers
VDAC
Negative markers
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23161513    
Organism Homo sapiens
Experiment description Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS.
Authors "Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ."
Journal name Mol Cell Proteomics
Publication year 2012
Sample Colorectal cancer cells
Sample name Dks-8
Isolation/purification methods Differential centrifugation
Filtration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
7
Experiment ID 209
MISEV standards
Biophysical techniques
TSG101|HSP70|FLOT1
Enriched markers
VDAC
Negative markers
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23161513    
Organism Homo sapiens
Experiment description Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS.
Authors "Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ."
Journal name Mol Cell Proteomics
Publication year 2012
Sample Colorectal cancer cells
Sample name DLD-1
Isolation/purification methods Differential centrifugation
Filtration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
8
Experiment ID 488
MISEV standards
EM
Biophysical techniques
CD9|CD81|CD63|GAPDH|SDCBP|LAMP1|TFRC|UCHL1|FLOT2|LAMP2|FLOT1|ICAM1|RAB5B|CD151|RAB35|TSG101|RAB5A|CD82
Enriched markers
CANX
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Homo sapiens
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Mesenchymal stem cells
Sample name UCMSC
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
9
Experiment ID 217
MISEV standards
EM
Biophysical techniques
TSG101|CD81|CD9|CD63
Enriched markers
Negative markers
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23844026    
Organism Homo sapiens
Experiment description Characterization of human thymic exosomes.
Authors "Skogberg G, Gudmundsdottir J, van der Post S, Sandstrom K, Bruhn S, Benson M, Mincheva-Nilsson L, Baranov V, Telemo E, Ekwall O."
Journal name PLoS One
Publication year 2013
Sample Thymus
Sample name Normal-Thymus
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for GTPBP1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 ATG16L1 55054
Affinity Capture-MS Homo sapiens
2 DUSP19  
Affinity Capture-MS Homo sapiens
3 HECTD1 25831
Affinity Capture-MS Homo sapiens
4 Gspt1 14852
Affinity Capture-MS Mus musculus
5 UBE2H 7328
Affinity Capture-MS Homo sapiens
6 MINK1 50488
Affinity Capture-MS Homo sapiens
7 RAD21 5885
Affinity Capture-Western Homo sapiens
8 MYCN  
Affinity Capture-MS Homo sapiens
9 FAM175B 23172
Affinity Capture-MS Homo sapiens
10 KIF3A 11127
Affinity Capture-MS Homo sapiens
11 Cit 12704
Affinity Capture-MS Mus musculus
12 FBXL6  
Affinity Capture-MS Homo sapiens
13 TP53 7157
Affinity Capture-MS Homo sapiens
14 EIF2S3 1968
Co-fractionation Homo sapiens
15 CPEB1  
Proximity Label-MS Homo sapiens
16 ZEB1  
Affinity Capture-MS Homo sapiens
17 E2F4  
Affinity Capture-MS Homo sapiens
18 ISOC2  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
19 NF2 4771
Affinity Capture-MS Homo sapiens
20 RAB17 64284
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
21 CHAMP1  
Affinity Capture-MS Homo sapiens
22 CUL3 8452
Affinity Capture-MS Homo sapiens
23 CPNE9  
Affinity Capture-MS Homo sapiens
24 CAND1 55832
Affinity Capture-MS Homo sapiens
25 PARK2  
Affinity Capture-MS Homo sapiens
26 PTK2 5747
Proximity Label-MS Homo sapiens
27 PLEKHA4 57664
Affinity Capture-MS Homo sapiens
28 DDX58 23586
Affinity Capture-RNA Homo sapiens
29 CORO1C 23603
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
30 VASP 7408
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
31 APP 351
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
32 NXF1 10482
Co-fractionation Homo sapiens
33 UFL1 23376
Affinity Capture-MS Homo sapiens
34 STK11 6794
Negative Genetic Homo sapiens
35 CER1  
Affinity Capture-MS Homo sapiens
36 SNRNP70 6625
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
37 EEF2KMT  
Affinity Capture-MS Homo sapiens
38 MYC  
Affinity Capture-MS Homo sapiens
39 IRF5  
Affinity Capture-MS Homo sapiens
40 NIN 51199
Proximity Label-MS Homo sapiens
41 PRC1 9055
Co-fractionation Homo sapiens
42 NTRK1 4914
Affinity Capture-MS Homo sapiens
43 UBE2O 63893
Affinity Capture-MS Homo sapiens
44 TRIM31  
Affinity Capture-MS Homo sapiens
45 Rrbp1  
Affinity Capture-MS Mus musculus
46 NHP2L1 4809
Co-fractionation Homo sapiens
47 COPS5 10987
Affinity Capture-MS Homo sapiens
48 TULP3 7289
Affinity Capture-MS Homo sapiens
49 DDRGK1 65992
Affinity Capture-MS Homo sapiens
50 LOX 4015
Affinity Capture-MS Homo sapiens
51 Rpl35 66489
Affinity Capture-MS Mus musculus
52 FSCN1 6624
Affinity Capture-MS Homo sapiens
53 Eif3e 16341
Affinity Capture-MS Mus musculus
54 RPA3 6119
Proximity Label-MS Homo sapiens
55 CEBPA  
Protein-peptide Homo sapiens
56 RAB27A 5873
Affinity Capture-MS Homo sapiens
57 HDGF 3068
Affinity Capture-MS Homo sapiens
58 MEX3C 51320
Affinity Capture-MS Homo sapiens
59 CDC5L 988
Affinity Capture-MS Homo sapiens
View the network image/svg+xml
 Pathways in which GTPBP1 is involved
No pathways found





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