Gene description for TRMT112
Gene name tRNA methyltransferase 11-2 homolog (S. cerevisiae)
Gene symbol TRMT112
Other names/aliases HSPC152
HSPC170
TRM112
TRMT11-2
Species Homo sapiens
 Database cross references - TRMT112
ExoCarta ExoCarta_51504
Vesiclepedia VP_51504
Entrez Gene 51504
HGNC 26940
UniProt Q9UI30  
 TRMT112 identified in exosomes derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Gastric cancer cells 33991177    
Gastric cancer cells 33991177    
Gastric cancer cells 33991177    
Mesenchymal stem cells 36408942    
Thymus 23844026    
 Gene ontology annotations for TRMT112
Molecular Function
    protein binding GO:0005515 IPI
    protein methyltransferase activity GO:0008276 IDA
    protein heterodimerization activity GO:0046982 IDA
    tRNA methyltransferase activator activity GO:0141106 IBA
    tRNA methyltransferase activator activity GO:0141106 IDA
Biological Process
    maturation of LSU-rRNA GO:0000470 IBA
    peptidyl-glutamine methylation GO:0018364 IDA
    tRNA methylation GO:0030488 IMP
    maturation of SSU-rRNA GO:0030490 IBA
    rRNA methylation GO:0031167 IDA
    transcription initiation-coupled chromatin remodeling GO:0045815 IDA
    rRNA (guanine-N7)-methylation GO:0070476 IMP
    positive regulation of rRNA processing GO:2000234 IBA
    positive regulation of rRNA processing GO:2000234 IMP
Subcellular Localization
    nucleus GO:0005634 IBA
    nucleoplasm GO:0005654 IDA
    nucleoplasm GO:0005654 TAS
    cytoplasm GO:0005737 IBA
    cytosol GO:0005829 TAS
    protein-containing complex GO:0032991 IDA
    tRNA (m2G10) methyltransferase complex GO:0043528 IBA
    perinuclear region of cytoplasm GO:0048471 IDA
 Experiment description of studies that identified TRMT112 in exosomes
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 363
MISEV standards
✘ Biophysical techniques
✔
TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1
Enriched markers
✔
DCLK1
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 33991177    
Organism Homo sapiens
Experiment description Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells
Authors "Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M."
Journal name Proteomics
Publication year 2021
Sample Gastric cancer cells
Sample name MKN1 - 100K pellet
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
6
Experiment ID 364
MISEV standards
✘ Biophysical techniques
✔
TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1
Enriched markers
✔
DCLK1
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 33991177    
Organism Homo sapiens
Experiment description Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells
Authors "Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M."
Journal name Proteomics
Publication year 2021
Sample Gastric cancer cells
Sample name MKN1 - 100K pellet
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
7
Experiment ID 365
MISEV standards
✘ Biophysical techniques
✔
TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1
Enriched markers
✔
DCLK1
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 33991177    
Organism Homo sapiens
Experiment description Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells
Authors "Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M."
Journal name Proteomics
Publication year 2021
Sample Gastric cancer cells
Sample name MKN1 - 100K pellet
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
8
Experiment ID 488
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD81|CD63|GAPDH|SDCBP|LAMP1|TFRC|UCHL1|FLOT2|LAMP2|FLOT1|ICAM1|RAB5B|CD151|RAB35|TSG101|RAB5A|CD82
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Homo sapiens
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Mesenchymal stem cells
Sample name UCMSC
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
9
Experiment ID 217
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|CD81|CD9|CD63
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23844026    
Organism Homo sapiens
Experiment description Characterization of human thymic exosomes.
Authors "Skogberg G, Gudmundsdottir J, van der Post S, Sandstrom K, Bruhn S, Benson M, Mincheva-Nilsson L, Baranov V, Telemo E, Ekwall O."
Journal name PLoS One
Publication year 2013
Sample Thymus
Sample name Normal-Thymus
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for TRMT112
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 RPL11 6135
Affinity Capture-MS Homo sapiens
2 ATG16L1 55054
Affinity Capture-MS Homo sapiens
3 SCOC 60592
Co-fractionation Homo sapiens
4 DHX15 1665
Co-fractionation Homo sapiens
5 NAPRT 93100
Co-fractionation Homo sapiens
6 SRSF11 9295
Co-fractionation Homo sapiens
7 MECP2 4204
Affinity Capture-MS Homo sapiens
8 Eif3a 13669
Affinity Capture-MS Mus musculus
9 PLEKHA4 57664
Affinity Capture-MS Homo sapiens
10 RBM39 9584
Co-fractionation Homo sapiens
11 THOC1 9984
Co-fractionation Homo sapiens
12 NCSTN 23385
Co-fractionation Homo sapiens
13 UBE2H 7328
Affinity Capture-MS Homo sapiens
14 RBM14 10432
Co-fractionation Homo sapiens
15 THUMPD3 25917
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
16 FAS 355
Proximity Label-MS Homo sapiens
17 IPO7 10527
Co-fractionation Homo sapiens
18 RIT1 6016
Negative Genetic Homo sapiens
19 DYNC1I2 1781
Proximity Label-MS Homo sapiens
20 WBSCR22  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
21 RLIM 51132
Affinity Capture-MS Homo sapiens
22 SRSF1 6426
Co-fractionation Homo sapiens
23 BYSL 705
Co-crystal Structure Homo sapiens
24 NOP56 10528
Co-fractionation Homo sapiens
25 EFNA3  
Affinity Capture-MS Homo sapiens
26 RPL10 6134
Affinity Capture-MS Homo sapiens
27 HIST1H4A 8359
Affinity Capture-MS Homo sapiens
28 SRSF5 6430
Co-fractionation Homo sapiens
29 THOC7 80145
Co-fractionation Homo sapiens
30 CAND1 55832
Co-fractionation Homo sapiens
31 Actb 11461
Affinity Capture-MS Mus musculus
32 PRPF40A 55660
Co-fractionation Homo sapiens
33 METTL5  
Two-hybrid Homo sapiens
34 SRSF3 6428
Co-fractionation Homo sapiens
35 SART1 9092
Co-fractionation Homo sapiens
36 KLF16  
Affinity Capture-MS Homo sapiens
37 KLF8  
Affinity Capture-MS Homo sapiens
38 RIOK1 83732
Co-crystal Structure Homo sapiens
39 ACIN1 22985
Co-fractionation Homo sapiens
40 SF3A1 10291
Co-fractionation Homo sapiens
41 UFL1 23376
Affinity Capture-MS Homo sapiens
42 SAFB 6294
Co-fractionation Homo sapiens
43 Arhgap6  
Affinity Capture-MS Mus musculus
44 Srp72  
Affinity Capture-MS Mus musculus
45 TRMT11 60487
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
46 IPO4 79711
Co-fractionation Homo sapiens
47 Inpp5b  
Affinity Capture-MS Mus musculus
48 EGFR 1956
Negative Genetic Homo sapiens
49 SF3B1 23451
Co-fractionation Homo sapiens
50 Rrbp1  
Affinity Capture-MS Mus musculus
51 SYNCRIP 10492
Affinity Capture-MS Homo sapiens
52 MEOX2  
Two-hybrid Homo sapiens
53 SRSF10 10772
Co-fractionation Homo sapiens
54 NPM1 4869
Affinity Capture-MS Homo sapiens
55 C9orf72  
Affinity Capture-MS Homo sapiens
56 FAM207A  
Affinity Capture-MS Homo sapiens
Co-crystal Structure Homo sapiens
57 Rpl35 66489
Affinity Capture-MS Mus musculus
58 CUL2 8453
Affinity Capture-MS Homo sapiens
59 Eif3e 16341
Affinity Capture-MS Mus musculus
60 KRAS 3845
Negative Genetic Homo sapiens
61 LUC7L3 51747
Co-fractionation Homo sapiens
62 RGS20 8601
Affinity Capture-MS Homo sapiens
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