Gene description for THUMPD3
Gene name THUMP domain containing 3
Gene symbol THUMPD3
Other names/aliases -
Species Homo sapiens
 Database cross references - THUMPD3
ExoCarta ExoCarta_25917
Vesiclepedia VP_25917
Entrez Gene 25917
HGNC 24493
UniProt Q9BV44  
 THUMPD3 identified in exosomes derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Mesenchymal stem cells 36408942    
 Gene ontology annotations for THUMPD3
Molecular Function
    tRNA binding GO:0000049 IEA
    protein binding GO:0005515 IPI
    tRNA (guanine) methyltransferase activity GO:0016423 IBA
    tRNA (guanine(6)-N2)-methyltransferase activity GO:0160117 IMP
    tRNA (guanine(7)-N2)-methyltransferase activity GO:0160118 IMP
Biological Process
    tRNA methylation GO:0030488 IBA
    tRNA methylation GO:0030488 IMP
Subcellular Localization
    nucleolus GO:0005730 IDA
    cytoplasm GO:0005737 IDA
    cytosol GO:0005829 IDA
 Experiment description of studies that identified THUMPD3 in exosomes
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 488
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD81|CD63|GAPDH|SDCBP|LAMP1|TFRC|UCHL1|FLOT2|LAMP2|FLOT1|ICAM1|RAB5B|CD151|RAB35|TSG101|RAB5A|CD82
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Homo sapiens
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Mesenchymal stem cells
Sample name UCMSC
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
 Protein-protein interactions for THUMPD3
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 UGGT2 55757
Affinity Capture-MS Homo sapiens
2 DDX5 1655
Co-fractionation Homo sapiens
3 VPS35 55737
Co-fractionation Homo sapiens
4 PLEKHA4 57664
Affinity Capture-MS Homo sapiens
5 GAGE1  
Affinity Capture-MS Homo sapiens
6 LSM8 51691
Two-hybrid Homo sapiens
7 CTU1 90353
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
8 D2HGDH 728294
Affinity Capture-MS Homo sapiens
9 THUMPD1 55623
Co-fractionation Homo sapiens
Co-fractionation Homo sapiens
10 MICU3  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
11 AVIL  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
12 L3HYPDH 112849
Affinity Capture-MS Homo sapiens
13 NSUN3  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
14 CTNND2 1501
Affinity Capture-MS Homo sapiens
15 LIN28A  
Affinity Capture-MS Homo sapiens
16 NAPA 8775
Co-fractionation Homo sapiens
17 DNAJA2 10294
Affinity Capture-MS Homo sapiens
18 PPM1B 5495
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
19 GRSF1 2926
Proximity Label-MS Homo sapiens
20 ACOT11 26027
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
21 FAM96A  
Affinity Capture-MS Homo sapiens
22 EN1  
Affinity Capture-MS Homo sapiens
23 SULT1C4  
Affinity Capture-MS Homo sapiens
24 GATC  
Affinity Capture-MS Homo sapiens
25 PRG2  
Affinity Capture-MS Homo sapiens
26 CCT8L2  
Affinity Capture-MS Homo sapiens
27 ZFR 51663
Co-fractionation Homo sapiens
28 TSEN2  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
29 SSB 6741
Co-fractionation Homo sapiens
30 PLCG1 5335
Co-fractionation Homo sapiens
31 CTU2 348180
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
32 PARK2  
Affinity Capture-MS Homo sapiens
33 C16orf72 29035
Affinity Capture-MS Homo sapiens
34 XPO7 23039
Co-fractionation Homo sapiens
35 METTL21B  
Affinity Capture-MS Homo sapiens
36 SLAMF1 6504
Affinity Capture-MS Homo sapiens
37 ADAM19 8728
Affinity Capture-MS Homo sapiens
38 DCTN3 11258
Affinity Capture-MS Homo sapiens
39 APOBEC3C 27350
Affinity Capture-MS Homo sapiens
40 APEX1 328
Affinity Capture-RNA Homo sapiens
41 ZNF501  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
42 VPS26A 9559
Co-fractionation Homo sapiens
43 PRR3  
Affinity Capture-MS Homo sapiens
44 SEC24D 9871
Co-fractionation Homo sapiens
45 ARHGAP25  
Affinity Capture-MS Homo sapiens
46 USP46 64854
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
47 TRMU  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
48 FXR2 9513
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
49 RBM3 5935
Affinity Capture-MS Homo sapiens
50 NTRK1 4914
Affinity Capture-MS Homo sapiens
51 LMNA 4000
Co-fractionation Homo sapiens
52 MICU1 10367
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
53 LECT2  
Affinity Capture-MS Homo sapiens
54 TRMT112 51504
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
55 ZNF653  
Affinity Capture-MS Homo sapiens
56 FAM114A1 92689
Co-fractionation Homo sapiens
57 CAMK2D 817
Affinity Capture-MS Homo sapiens
58 EDRF1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
59 TGM1 7051
Affinity Capture-MS Homo sapiens
60 ZC3H14  
Co-fractionation Homo sapiens
61 CKAP4 10970
Proximity Label-MS Homo sapiens
62 METTL5  
Co-fractionation Homo sapiens
63 GRK7  
Affinity Capture-MS Homo sapiens
64 PUS1 80324
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
65 GTSF1L  
Affinity Capture-MS Homo sapiens
66 EFNB3 1949
Affinity Capture-MS Homo sapiens
67 EXD2  
Proximity Label-MS Homo sapiens
68 HUWE1 10075
Affinity Capture-MS Homo sapiens
69 CARS 833
Co-fractionation Homo sapiens
70 ESRP1 54845
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
View the network image/svg+xml
 Pathways in which THUMPD3 is involved
No pathways found





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