Gene description for LUC7L3
Gene name LUC7-like 3 (S. cerevisiae)
Gene symbol LUC7L3
Other names/aliases CRA
CREAP-1
CROP
LUC7A
OA48-18
hLuc7A
Species Homo sapiens
 Database cross references - LUC7L3
ExoCarta ExoCarta_51747
Vesiclepedia VP_51747
Entrez Gene 51747
HGNC 24309
MIM 609434
UniProt O95232  
 LUC7L3 identified in sEVs derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Chondrocytes 35931686    
Chondrocytes 35931686    
Gastric cancer cells 33991177    
Gastric cancer cells 33991177    
Gastric cancer cells 33991177    
Mesenchymal stem cells 36408942    
Retinal pigment epithelial cells 35333565    
Retinal pigment epithelial cells 35333565    
 Gene ontology annotations for LUC7L3
Molecular Function
    DNA binding GO:0003677 IEA
    RNA binding GO:0003723 HDA
    mRNA binding GO:0003729 IBA
    mRNA binding GO:0003729 IMP
    protein binding GO:0005515 IPI
Biological Process
    mRNA splice site recognition GO:0006376 IBA
    RNA splicing GO:0008380 IMP
Subcellular Localization
    nucleus GO:0005634 IDA
    nucleoplasm GO:0005654 IDA
    nucleoplasm GO:0005654 TAS
    U1 snRNP GO:0005685 IBA
    nuclear speck GO:0016607 IDA
    U2-type prespliceosome GO:0071004 IBA
 Experiment description of studies that identified LUC7L3 in sEVs
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 497
MISEV standards
✘ Biophysical techniques
✔
CD9|CD151|CD63|CD81|CD82|FLOT1|FLOT2|GAPDH|LAMP1|LAMP2|RAB5A|SDCBP|TFRC|TSG101|UCHL1
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35931686    
Organism Homo sapiens
Experiment description Extracellular vesicles enriched in connexin 43 promote a senescent phenotype in bone and synovial cells contributing to osteoarthritis progression
Authors "Varela-Eirín M, Carpintero-Fernández P, Guitián-Caamaño A, Varela-Vázquez A, García-Yuste A, Sánchez-Temprano A, Bravo-López SB, Yañez-Cabanas J, Fonseca E, Largo R, Mobasheri A, Caeiro JR, Mayán MD."
Journal name Cell Death Dis
Publication year 2022
Sample Chondrocytes
Sample name T/C-28a2
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
6
Experiment ID 498
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD151|CD63|CD81|CD82|FLOT1|FLOT2|GAPDH|LAMP1|LAMP2|RAB5A|SDCBP|TFRC|TSG101|UCHL1
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35931686    
Organism Homo sapiens
Experiment description Extracellular vesicles enriched in connexin 43 promote a senescent phenotype in bone and synovial cells contributing to osteoarthritis progression
Authors "Varela-Eirín M, Carpintero-Fernández P, Guitián-Caamaño A, Varela-Vázquez A, García-Yuste A, Sánchez-Temprano A, Bravo-López SB, Yañez-Cabanas J, Fonseca E, Largo R, Mobasheri A, Caeiro JR, Mayán MD."
Journal name Cell Death Dis
Publication year 2022
Sample Chondrocytes
Sample name T/C-28a2
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
7
Experiment ID 363
MISEV standards
✘ Biophysical techniques
✔
TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1
Enriched markers
✔
DCLK1
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 33991177    
Organism Homo sapiens
Experiment description Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells
Authors "Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M."
Journal name Proteomics
Publication year 2021
Sample Gastric cancer cells
Sample name MKN1 - 100K pellet
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
8
Experiment ID 364
MISEV standards
✘ Biophysical techniques
✔
TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1
Enriched markers
✔
DCLK1
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 33991177    
Organism Homo sapiens
Experiment description Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells
Authors "Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M."
Journal name Proteomics
Publication year 2021
Sample Gastric cancer cells
Sample name MKN1 - 100K pellet
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
9
Experiment ID 365
MISEV standards
✘ Biophysical techniques
✔
TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1
Enriched markers
✔
DCLK1
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 33991177    
Organism Homo sapiens
Experiment description Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells
Authors "Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M."
Journal name Proteomics
Publication year 2021
Sample Gastric cancer cells
Sample name MKN1 - 100K pellet
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
10
Experiment ID 488
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD81|CD63|GAPDH|SDCBP|LAMP1|TFRC|UCHL1|FLOT2|LAMP2|FLOT1|ICAM1|RAB5B|CD151|RAB35|TSG101|RAB5A|CD82
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Homo sapiens
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Mesenchymal stem cells
Sample name UCMSC
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
11
Experiment ID 834
MISEV standards
✔
EM
Biophysical techniques
✔
LAMP2|CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P"
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
12
Experiment ID 835
MISEV standards
✔
EM
Biophysical techniques
✔
CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P"
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for LUC7L3
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 UBE2H 7328
Affinity Capture-MS Homo sapiens
2 RIT1 6016
Negative Genetic Homo sapiens
3 EML4 27436
Co-fractionation Homo sapiens
4 SRPK2 6733
Biochemical Activity Homo sapiens
Affinity Capture-MS Homo sapiens
5 MRPS27 23107
Co-fractionation Homo sapiens
6 DYNC1I2 1781
Co-fractionation Homo sapiens
7 RPA2 6118
Proximity Label-MS Homo sapiens
8 DHX8 1659
Proximity Label-MS Homo sapiens
9 KIF20A 10112
Affinity Capture-MS Homo sapiens
10 NABP2  
Affinity Capture-MS Homo sapiens
11 CAND1 55832
Affinity Capture-MS Homo sapiens
12 SNRPF 6636
Affinity Capture-MS Homo sapiens
13 SNRPD2 6633
Co-fractionation Homo sapiens
14 GRB7 2886
Affinity Capture-MS Homo sapiens
15 Rrp1b 72462
Affinity Capture-MS Mus musculus
16 DDX23 9416
Proximity Label-MS Homo sapiens
17 PARK2  
Affinity Capture-MS Homo sapiens
18 RNPS1 10921
Affinity Capture-MS Homo sapiens
Two-hybrid Homo sapiens
19 B3GNT2 10678
Affinity Capture-MS Homo sapiens
20 KIF23 9493
Affinity Capture-MS Homo sapiens
21 TRMT112 51504
Co-fractionation Homo sapiens
22 COPS5 10987
Affinity Capture-MS Homo sapiens
23 FBXW7  
Affinity Capture-MS Homo sapiens
24 PRC1 9055
Affinity Capture-MS Homo sapiens
25 SNRPC 6631
Affinity Capture-MS Homo sapiens
26 MTMR2 8898
Affinity Capture-MS Homo sapiens
27 OBSL1 23363
Affinity Capture-MS Homo sapiens
28 RCC1 1104
Co-fractionation Homo sapiens
29 EED  
Affinity Capture-MS Homo sapiens
30 MECP2 4204
Affinity Capture-MS Homo sapiens
31 YWHAG 7532
Affinity Capture-MS Homo sapiens
32 CUL1 8454
Affinity Capture-MS Homo sapiens
33 CTCF  
Co-fractionation Homo sapiens
34 KIF14 9928
Affinity Capture-MS Homo sapiens
35 UNK  
Affinity Capture-RNA Homo sapiens
36 NF2 4771
Affinity Capture-MS Homo sapiens
37 JMJD6 23210
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
38 HIST1H4A 8359
Affinity Capture-MS Homo sapiens
39 MEPCE 56257
Affinity Capture-MS Homo sapiens
40 PHAX  
Co-fractionation Homo sapiens
41 SMU1 55234
Co-fractionation Homo sapiens
42 NFIA 4774
Co-fractionation Homo sapiens
43 CHMP4B 128866
Affinity Capture-MS Homo sapiens
44 LRRFIP1 9208
Co-fractionation Homo sapiens
45 CEP170 9859
Co-fractionation Homo sapiens
46 BARD1 580
Affinity Capture-MS Homo sapiens
47 NTRK1 4914
Affinity Capture-MS Homo sapiens
48 RAD18  
Affinity Capture-MS Homo sapiens
49 DNAJA1 3301
Co-fractionation Homo sapiens
50 LUC7L2 51631
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
51 BANF1 8815
Co-fractionation Homo sapiens
52 TRIM11  
Affinity Capture-MS Homo sapiens
53 ENO1 2023
Affinity Capture-RNA Homo sapiens
54 CUL2 8453
Affinity Capture-MS Homo sapiens
55 RRP1B 23076
Affinity Capture-MS Homo sapiens
56 SRPK1 6732
Biochemical Activity Homo sapiens
57 ANLN 54443
Affinity Capture-MS Homo sapiens
58 OGT 8473
Reconstituted Complex Homo sapiens
59 ZBTB2 57621
Affinity Capture-MS Homo sapiens
60 EAF1  
Affinity Capture-MS Homo sapiens
61 ECT2 1894
Affinity Capture-MS Homo sapiens
62 SNRPB 6628
Affinity Capture-MS Homo sapiens
63 SRSF1 6426
Two-hybrid Homo sapiens
64 CUL3 8452
Affinity Capture-MS Homo sapiens
65 EGFR 1956
Affinity Capture-MS Homo sapiens
66 SUZ12  
Affinity Capture-MS Homo sapiens
67 SART1 9092
Co-fractionation Homo sapiens
68 NXF1 10482
Affinity Capture-RNA Homo sapiens
Co-fractionation Homo sapiens
69 STRAP 11171
Co-fractionation Homo sapiens
70 TADA2A  
Affinity Capture-MS Homo sapiens
71 RPA3 6119
Proximity Label-MS Homo sapiens
72 NLRP3  
Affinity Capture-MS Homo sapiens
73 AKAP5  
Affinity Capture-MS Homo sapiens
74 DDB1 1642
Co-fractionation Homo sapiens
75 CUL7 9820
Affinity Capture-MS Homo sapiens
76 DDRGK1 65992
Affinity Capture-MS Homo sapiens
77 RNF2  
Affinity Capture-MS Homo sapiens
78 BMI1  
Affinity Capture-MS Homo sapiens
79 HAT1 8520
Co-fractionation Homo sapiens
80 HIST1H2AJ 8331
Co-fractionation Homo sapiens
81 PAIP1 10605
Co-fractionation Homo sapiens
82 PLEKHA4 57664
Affinity Capture-MS Homo sapiens
83 CLK1  
Biochemical Activity Homo sapiens
84 ZRANB2 9406
Affinity Capture-MS Homo sapiens
85 FBXL6  
Affinity Capture-MS Homo sapiens
86 FOLR1 2348
Affinity Capture-MS Homo sapiens
87 WWOX 51741
Affinity Capture-MS Homo sapiens
88 SNRPA 6626
Affinity Capture-MS Homo sapiens
89 PTTG1  
Synthetic Growth Defect Homo sapiens
90 RUVBL2 10856
Co-fractionation Homo sapiens
91 HDAC1 3065
Co-fractionation Homo sapiens
92 SHMT2 6472
Affinity Capture-RNA Homo sapiens
93 S100A9 6280
Co-fractionation Homo sapiens
94 DDX58 23586
Affinity Capture-RNA Homo sapiens
95 VTN 7448
Co-fractionation Homo sapiens
96 ESR1  
Affinity Capture-MS Homo sapiens
97 UFL1 23376
Affinity Capture-MS Homo sapiens
98 TXN 7295
Co-fractionation Homo sapiens
99 ITSN2 50618
Two-hybrid Homo sapiens
100 MOV10 4343
Affinity Capture-RNA Homo sapiens
101 YBX3 8531
Co-fractionation Homo sapiens
102 TRIM31  
Affinity Capture-MS Homo sapiens
103 C1QBP 708
Proximity Label-MS Homo sapiens
104 TXNIP 10628
Two-hybrid Homo sapiens
105 SNW1 22938
Affinity Capture-MS Homo sapiens
106 KRAS 3845
Negative Genetic Homo sapiens
107 HNRNPU 3192
Co-fractionation Homo sapiens
108 SYN1  
Affinity Capture-MS Homo sapiens
109 RC3H1 149041
Affinity Capture-MS Homo sapiens
110 SRSF7 6432
Co-fractionation Homo sapiens
111 PRMT1 3276
Affinity Capture-MS Homo sapiens
112 LUC7L 55692
Affinity Capture-MS Homo sapiens
113 LUC7L3 51747
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
View the network image/svg+xml



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