Gene description for GRB7
Gene name growth factor receptor-bound protein 7
Gene symbol GRB7
Other names/aliases -
Species Homo sapiens
 Database cross references - GRB7
ExoCarta ExoCarta_2886
Vesiclepedia VP_2886
Entrez Gene 2886
HGNC 4567
MIM 601522
UniProt Q14451  
 GRB7 identified in exosomes derived from the following tissue/cell type
Colorectal cancer cells 34887515    
Hepatocytes 26054723    
Mesenchymal stem cells 36408942    
 Gene ontology annotations for GRB7
Molecular Function
    phosphatidylinositol binding GO:0035091 IDA
    protein kinase binding GO:0019901 IDA
    RNA binding GO:0003723 IEA
    identical protein binding GO:0042802 IPI
    protein binding GO:0005515 IPI
    SH3/SH2 adaptor activity GO:0005070 TAS
Biological Process
    blood coagulation GO:0007596 TAS
    leukocyte migration GO:0050900 TAS
    stress granule assembly GO:0034063 ISS
    positive regulation of signal transduction GO:0009967 TAS
    epidermal growth factor receptor signaling pathway GO:0007173 TAS
    positive regulation of cell migration GO:0030335 IDA
    negative regulation of translation GO:0017148 ISS
Subcellular Localization
    cytosol GO:0005829 TAS
    cytoplasmic stress granule GO:0010494 ISS
    cell projection GO:0042995 IEA
    focal adhesion GO:0005925 IDA
    plasma membrane GO:0005886 IEA
 Experiment description of studies that identified GRB7 in exosomes
1
Experiment ID 1203
MISEV standards
✔
EM
EV Biophysical techniques
✔
SDCBP|FLOT1|CD9|CD81|CD63|EPCAM|GAPDH|LAMP1|TFRC|CD151|CD82|LAMP2|RAB35|TSG101|FLOT2|RAB5B|ICAM1|RAB5A
EV Enriched markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 34887515    
Organism Homo sapiens
Experiment description Supermeres are functional extracellular nanoparticles replete with disease biomarkers and therapeutic targets
Authors Zhang Q, Jeppesen DK, Higginbotham JN, Graves-Deal R, Trinh VQ, Ramirez MA, Sohn Y, Neininger AC, Taneja N, McKinley ET, Niitsu H, Cao Z, Evans R, Glass SE, Ray KC, Fissell WH, Hill S, Rose KL, Huh WJ, Washington MK, Ayers GD, Burnette DT, Sharma S, Rome LH, Franklin JL, Lee YA, Liu Q, Coffey RJ.
Journal name Nat Cell Biol
Publication year 2021
Sample Colorectal cancer cells
Sample name DiFi
Isolation/purification methods Differential centrifugation
Filtration
Centrifugal ultrafiltration
Ultracentrifugation
OptiPrep density gradient centrifugation
Flotation density -
Molecules identified in the study Protein
miRNA
Methods used in the study Western blotting
Mass spectrometry
RNA sequencing
2
Experiment ID 237
MISEV standards
✔
EM
EV Biophysical techniques
✔
TSG101|Alix|HSC70|GAPDH
EV Enriched markers
✔
HSP90B1
EV Negative markers
✔
qNano
EV Particle analysis
Identified molecule mRNA
Identification method RNA Sequencing
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocytes
Sample name MIHA
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RNA Sequencing
3
Experiment ID 488
MISEV standards
✔
EM
EV Biophysical techniques
✔
CD9|CD81|CD63|GAPDH|SDCBP|LAMP1|TFRC|UCHL1|FLOT2|LAMP2|FLOT1|ICAM1|RAB5B|CD151|RAB35|TSG101|RAB5A|CD82
EV Enriched markers
✔
CANX
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Homo sapiens
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ.
Journal name Proteomics
Publication year 2023
Sample Mesenchymal stem cells
Sample name UCMSC
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
 Protein-protein interactions for GRB7
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 C6orf21  
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
2 SFN 2810
Affinity Capture-MS Homo sapiens
3 KIT 3815
Reconstituted Complex Homo sapiens
4 ERBB2 2064
Invivo Homo sapiens
Invitro Homo sapiens
5 RET 5979
Invivo Homo sapiens
Invitro Homo sapiens
Affinity Capture-MS Homo sapiens
6 INSR 3643
Invivo Homo sapiens
Invitro Homo sapiens
Two-hybrid Homo sapiens
7 EGFR 1956
Invivo Homo sapiens
Invitro Homo sapiens
8 GRB2 2885
Affinity Capture-MS Homo sapiens
9 PTK2 5747
Invivo Homo sapiens
Invitro Homo sapiens
Affinity Capture-MS Homo sapiens
10 RND1  
Two-hybrid Homo sapiens
Reconstituted Complex Homo sapiens
11 ERBB3 2065
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
View the network image/svg+xml
 Pathways in which GRB7 is involved
PathwayEvidenceSource
Downstream signal transduction TAS Reactome
GRB7 events in ERBB2 signaling TAS Reactome
Signaling by SCF-KIT TAS Reactome
Tie2 Signaling TAS Reactome





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