Gene description for FLAD1
Gene name flavin adenine dinucleotide synthetase 1
Gene symbol FLAD1
Other names/aliases FAD1
FADS
PP591
Species Homo sapiens
 Database cross references - FLAD1
ExoCarta ExoCarta_80308
Vesiclepedia VP_80308
Entrez Gene 80308
HGNC 24671
MIM 610595
UniProt Q8NFF5  
 FLAD1 identified in exosomes derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Colorectal cancer cells 34887515    
Mesenchymal stem cells 36408942    
 Gene ontology annotations for FLAD1
Molecular Function
    FMN adenylyltransferase activity GO:0003919 IBA
    FMN adenylyltransferase activity GO:0003919 IDA
    FMN adenylyltransferase activity GO:0003919 TAS
    protein binding GO:0005515 IPI
    ATP binding GO:0005524 IEA
    identical protein binding GO:0042802 IPI
Biological Process
    FAD biosynthetic process GO:0006747 IBA
    FAD biosynthetic process GO:0006747 IDA
    FAD biosynthetic process GO:0006747 IEA
    riboflavin metabolic process GO:0006771 TAS
Subcellular Localization
    mitochondrion GO:0005739 HTP
    mitochondrial matrix GO:0005759 IDA
    cytosol GO:0005829 IDA
    cytosol GO:0005829 IDA
    cytosol GO:0005829 TAS
    plasma membrane GO:0005886 IDA
 Experiment description of studies that identified FLAD1 in exosomes
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 1203
MISEV standards
✔
EM
Biophysical techniques
✔
SDCBP|FLOT1|CD9|CD81|CD63|EPCAM|GAPDH|LAMP1|TFRC|CD151|CD82|LAMP2|RAB35|TSG101|FLOT2|RAB5B|ICAM1|RAB5A
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 34887515    
Organism Homo sapiens
Experiment description Supermeres are functional extracellular nanoparticles replete with disease biomarkers and therapeutic targets
Authors "Zhang Q, Jeppesen DK, Higginbotham JN, Graves-Deal R, Trinh VQ, Ramirez MA, Sohn Y, Neininger AC, Taneja N, McKinley ET, Niitsu H, Cao Z, Evans R, Glass SE, Ray KC, Fissell WH, Hill S, Rose KL, Huh WJ, Washington MK, Ayers GD, Burnette DT, Sharma S, Rome LH, Franklin JL, Lee YA, Liu Q, Coffey RJ."
Journal name Nat Cell Biol
Publication year 2021
Sample Colorectal cancer cells
Sample name DiFi
Isolation/purification methods Differential centrifugation
Filtration
Centrifugal ultrafiltration
Ultracentrifugation
OptiPrep density gradient centrifugation
Flotation density -
Molecules identified in the study Protein
miRNA
Methods used in the study Western blotting
Mass spectrometry
RNA sequencing
6
Experiment ID 488
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD81|CD63|GAPDH|SDCBP|LAMP1|TFRC|UCHL1|FLOT2|LAMP2|FLOT1|ICAM1|RAB5B|CD151|RAB35|TSG101|RAB5A|CD82
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Homo sapiens
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Mesenchymal stem cells
Sample name UCMSC
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
 Protein-protein interactions for FLAD1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 UBE2H 7328
Affinity Capture-MS Homo sapiens
2 TK1 7083
Two-hybrid Homo sapiens
3 GRB7 2886
Two-hybrid Homo sapiens
4 PDE6H  
Affinity Capture-MS Homo sapiens
5 FOXD3  
Affinity Capture-MS Homo sapiens
6 REL 5966
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
7 OTP  
Affinity Capture-MS Homo sapiens
8 TRUB2  
Proximity Label-MS Homo sapiens
9 ISCA1  
Affinity Capture-MS Homo sapiens
10 CELA2A  
Affinity Capture-MS Homo sapiens
11 ANKRD39  
Affinity Capture-MS Homo sapiens
12 ST7  
Affinity Capture-MS Homo sapiens
13 SOX2  
Affinity Capture-MS Homo sapiens
14 RB1CC1 9821
Affinity Capture-MS Homo sapiens
15 CCL18  
Affinity Capture-MS Homo sapiens
16 GOLGA6L2  
Affinity Capture-MS Homo sapiens
17 PMPCA 23203
Proximity Label-MS Homo sapiens
18 SPANXN4  
Affinity Capture-MS Homo sapiens
19 HINT2 84681
Proximity Label-MS Homo sapiens
20 FSCN3  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
21 MRPS26 64949
Proximity Label-MS Homo sapiens
22 TULP3 7289
Affinity Capture-MS Homo sapiens
23 DUS1L 64118
Affinity Capture-MS Homo sapiens
24 SDCBP 6386
Two-hybrid Homo sapiens
25 VWA8 23078
Proximity Label-MS Homo sapiens
26 NIF3L1 60491
Two-hybrid Homo sapiens
27 PSPH 5723
Co-fractionation Homo sapiens
28 MDH2 4191
Proximity Label-MS Homo sapiens
29 ANXA7 310
Two-hybrid Homo sapiens
30 C6orf203  
Proximity Label-MS Homo sapiens
31 CDKN1A  
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
32 GFM1 85476
Proximity Label-MS Homo sapiens
33 TUFM 7284
Proximity Label-MS Homo sapiens
34 FAM19A3  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
35 MOV10 4343
Affinity Capture-RNA Homo sapiens
36 BICD2 23299
Affinity Capture-MS Homo sapiens
37 PLEKHO2 80301
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
38 COPS3 8533
Two-hybrid Homo sapiens
39 APTX  
Two-hybrid Homo sapiens
40 KLHL14 57565
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
41 MRRF  
Proximity Label-MS Homo sapiens
42 UBAP2 55833
Affinity Capture-MS Homo sapiens
43 MTIF3  
Proximity Label-MS Homo sapiens
44 RCC1 1104
Two-hybrid Homo sapiens
45 CS 1431
Proximity Label-MS Homo sapiens
46 CUL3 8452
Affinity Capture-MS Homo sapiens
47 SCGB1C1  
Affinity Capture-MS Homo sapiens
48 SORD 6652
Co-fractionation Homo sapiens
49 TNRC6B  
Affinity Capture-MS Homo sapiens
50 NXF1 10482
Affinity Capture-RNA Homo sapiens
51 GEMIN4 50628
Two-hybrid Homo sapiens
52 L3MBTL3  
Two-hybrid Homo sapiens
53 RPA3 6119
Proximity Label-MS Homo sapiens
54 PTPN1 5770
Affinity Capture-MS Homo sapiens
55 ZNF689  
Affinity Capture-MS Homo sapiens
56 ACAD9 28976
Proximity Label-MS Homo sapiens
57 TKFC 26007
Co-fractionation Homo sapiens
58 MCUR1 63933
Proximity Label-MS Homo sapiens
59 PFAS 5198
Co-fractionation Homo sapiens
60 C6orf222  
Affinity Capture-MS Homo sapiens
61 CLPP 8192
Proximity Label-MS Homo sapiens
62 NUDT21 11051
Two-hybrid Homo sapiens
63 PLEKHA4 57664
Affinity Capture-MS Homo sapiens
64 TSFM 10102
Proximity Label-MS Homo sapiens
65 AUH 549
Proximity Label-MS Homo sapiens
66 SMN1 6606
Two-hybrid Homo sapiens
67 PSG1  
Affinity Capture-MS Homo sapiens
68 ICT1 3396
Affinity Capture-MS Homo sapiens
69 CRABP2 1382
Two-hybrid Homo sapiens
70 APEX1 328
Affinity Capture-RNA Homo sapiens
71 UBA52 7311
Affinity Capture-MS Homo sapiens
72 PDHA1 5160
Proximity Label-MS Homo sapiens
73 PRTFDC1 56952
Two-hybrid Homo sapiens
74 COX8A  
Proximity Label-MS Homo sapiens
75 C21orf33  
Proximity Label-MS Homo sapiens
76 CLVS2 134829
Two-hybrid Homo sapiens
77 TCF4  
Two-hybrid Homo sapiens
78 TBRG4 9238
Proximity Label-MS Homo sapiens
79 AARS2  
Proximity Label-MS Homo sapiens
80 TRIM23 373
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
81 C16orf72 29035
Affinity Capture-MS Homo sapiens
82 SH3GLB1 51100
Co-fractionation Homo sapiens
83 C1QBP 708
Proximity Label-MS Homo sapiens
84 SERBP1 26135
Affinity Capture-MS Homo sapiens
85 METTL17  
Proximity Label-MS Homo sapiens
86 FLAD1 80308
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
View the network image/svg+xml



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