Experiment description of studies that identified UBAP2 in exosomes

1 Experiment ID 426 MISEV standards ✘ Biophysical techniques ✔ SDCBP|FLOT1|CD81|CD9|CD151|FLOT2|TSG101|LAMP1|TFRC|RAB5A|RAB35|GAPDH|UCHL1|ICAM1 Enriched markers ✔ CANX Negative markers ✘ Particle analysis Identified molecule Protein Identification method Mass spectrometry PubMed ID 34108659     Organism Homo sapiens Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R." Journal name Nat Cell Biol Publication year 2021 Sample Breast cancer cells Sample name MDA-MB-231 - Exo-rich fractions 7-10 pooled Isolation/purification methods Differential centrifugation Filtration Ultracentrifugation Size exclusion chromatography Flotation density - Molecules identified in the study Protein Methods used in the study Western blotting Mass spectrometry

2 Experiment ID 427 MISEV standards ✘ Biophysical techniques ✔ CD81|SDCBP|FLOT1|CD9|CD151|FLOT2|TSG101|LAMP1|TFRC|RAB5A|RAB35|GAPDH|UCHL1|ICAM1 Enriched markers ✔ CANX Negative markers ✘ Particle analysis Identified molecule Protein Identification method Mass spectrometry PubMed ID 34108659     Organism Homo sapiens Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R." Journal name Nat Cell Biol Publication year 2021 Sample Breast cancer cells Sample name MDA-MB-231 - Exo-rich fractions 1-6 pooled Isolation/purification methods Differential centrifugation Filtration Ultracentrifugation OptiPrep density gradient centrifugation Flotation density - Molecules identified in the study Protein Methods used in the study Mass spectrometry

3 Experiment ID 419 MISEV standards ✘ Biophysical techniques ✔ SDCBP|FLOT1|CD81|CD9|CD151|FLOT2|TSG101|LAMP1|TFRC|RAB5A|RAB35|GAPDH|UCHL1|ICAM1 Enriched markers ✔ CANX Negative markers ✘ Particle analysis Identified molecule Protein Identification method Mass spectrometry PubMed ID 34108659     Organism Homo sapiens Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R." Journal name Nat Cell Biol Publication year 2021 Sample Embryonic kidney cells Sample name HEK293T - Exo-rich fractions 7-10 pooled Isolation/purification methods Differential centrifugation Filtration Ultracentrifugation Size exclusion chromatography Flotation density - Molecules identified in the study Protein Methods used in the study Western blotting Mass spectrometry

4 Experiment ID 419 MISEV standards ✘ Biophysical techniques ✔ SDCBP|FLOT1|CD81|CD9|CD151|FLOT2|TSG101|LAMP1|TFRC|RAB5A|RAB35|GAPDH|UCHL1|ICAM1 Enriched markers ✔ CANX Negative markers ✘ Particle analysis Identified molecule Protein Identification method Mass spectrometry PubMed ID 34108659     Organism Homo sapiens Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R." Journal name Nat Cell Biol Publication year 2021 Sample Embryonic kidney cells Sample name HEK293T - Exo-rich fractions 7-10 pooled Isolation/purification methods Differential centrifugation Filtration Ultracentrifugation Size exclusion chromatography Flotation density - Molecules identified in the study Protein Methods used in the study Western blotting Mass spectrometry

5 Experiment ID 420 MISEV standards ✘ Biophysical techniques ✔ CD81|SDCBP|FLOT1|CD9|CD151|FLOT2|TSG101|LAMP1|TFRC|RAB5A|RAB35|GAPDH|UCHL1|ICAM1 Enriched markers ✔ CANX Negative markers ✘ Particle analysis Identified molecule Protein Identification method Mass spectrometry PubMed ID 34108659     Organism Homo sapiens Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R." Journal name Nat Cell Biol Publication year 2021 Sample Embryonic kidney cells Sample name HEK293T - Exo-rich fractions 1-6 pooled Isolation/purification methods Differential centrifugation Filtration Ultracentrifugation OptiPrep density gradient centrifugation Flotation density - Molecules identified in the study Protein Methods used in the study Mass spectrometry

6 Experiment ID 420 MISEV standards ✘ Biophysical techniques ✔ CD81|SDCBP|FLOT1|CD9|CD151|FLOT2|TSG101|LAMP1|TFRC|RAB5A|RAB35|GAPDH|UCHL1|ICAM1 Enriched markers ✔ CANX Negative markers ✘ Particle analysis Identified molecule Protein Identification method Mass spectrometry PubMed ID 34108659     Organism Homo sapiens Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R." Journal name Nat Cell Biol Publication year 2021 Sample Embryonic kidney cells Sample name HEK293T - Exo-rich fractions 1-6 pooled Isolation/purification methods Differential centrifugation Filtration Ultracentrifugation OptiPrep density gradient centrifugation Flotation density - Molecules identified in the study Protein Methods used in the study Mass spectrometry

7 Experiment ID 434 MISEV standards ✘ Biophysical techniques ✔ SDCBP|FLOT1|CD81|CD9|CD151|FLOT2|TSG101|LAMP1|TFRC|RAB5A|RAB35|GAPDH|UCHL1|ICAM1 Enriched markers ✔ CANX Negative markers ✘ Particle analysis Identified molecule Protein Identification method Mass spectrometry PubMed ID 34108659     Organism Homo sapiens Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R." Journal name Nat Cell Biol Publication year 2021 Sample Pancreatic cancer cells Sample name PANC-1 - Exo-rich fractions 7-10 pooled Isolation/purification methods Differential centrifugation Filtration Ultracentrifugation Size exclusion chromatography Flotation density - Molecules identified in the study Protein Methods used in the study Western blotting Mass spectrometry

8 Experiment ID 435 MISEV standards ✘ Biophysical techniques ✔ CD81|SDCBP|FLOT1|CD9|CD151|FLOT2|TSG101|LAMP1|TFRC|RAB5A|RAB35|GAPDH|UCHL1|ICAM1 Enriched markers ✔ CANX Negative markers ✘ Particle analysis Identified molecule Protein Identification method Mass spectrometry PubMed ID 34108659     Organism Homo sapiens Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R." Journal name Nat Cell Biol Publication year 2021 Sample Pancreatic cancer cells Sample name PANC-1 - Exo-rich fractions 1-6 pooled Isolation/purification methods Differential centrifugation Filtration Ultracentrifugation OptiPrep density gradient centrifugation Flotation density - Molecules identified in the study Protein Methods used in the study Mass spectrometry

Protein-protein interactions for UBAP2

Protein Interactor ExoCarta ID Identification method PubMed Species 1 HNRNPH1 3187 Affinity Capture-RNA Homo sapiens 2 UBE2H 7328 Affinity Capture-MS Homo sapiens 3 ISX   Two-hybrid Homo sapiens 4 RBPMS 11030 Two-hybrid Homo sapiens 5 AES 166 Two-hybrid Homo sapiens 6 ZC3H14   Co-fractionation Homo sapiens 7 MGAT5B   Two-hybrid Homo sapiens 8 C9orf78 51759 Affinity Capture-MS Homo sapiens 9 ZFR 51663 Co-fractionation Homo sapiens 10 Actb 11461 Affinity Capture-MS Mus musculus 11 PSMB8 5696 Two-hybrid Homo sapiens 12 VPS29 51699 Co-fractionation Homo sapiens 13 RPS20 6224 Proximity Label-MS Homo sapiens 14 HSPA2 3306 Affinity Capture-MS Homo sapiens 15 PLAC8   Two-hybrid Homo sapiens 16 HGS 9146 Two-hybrid Homo sapiens 17 STX7 8417 Co-fractionation Homo sapiens 18 FBL 2091 Proximity Label-MS Homo sapiens 19 TOMM40 10452 Co-fractionation Homo sapiens 20 KLHL20   Affinity Capture-MS Homo sapiens 21 TEX37   Two-hybrid Homo sapiens 22 CPEB1   Proximity Label-MS Homo sapiens 23 HSPBP1 23640 Affinity Capture-MS Homo sapiens 24 BANP   Two-hybrid Homo sapiens 25 DLD 1738 Co-fractionation Homo sapiens 26 PRDX5 25824 Co-fractionation Homo sapiens 27 KRTAP15-1   Two-hybrid Homo sapiens 28 WWP2 11060 Affinity Capture-MS Homo sapiens 29 UBAP2L 9898 Co-fractionation Homo sapiens 30 RPA4   Proximity Label-MS Homo sapiens 31 DAZAP2   Two-hybrid Homo sapiens 32 PLEKHB2 55041 Two-hybrid Homo sapiens 33 CDH1 999 Proximity Label-MS Homo sapiens 34 HAO2   Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 35 NTRK1 4914 Affinity Capture-MS Homo sapiens 36 GORASP2 26003 Affinity Capture-MS Homo sapiens 37 C10orf55   Two-hybrid Homo sapiens 38 PPP2CA 5515 Two-hybrid Homo sapiens 39 NOXA1   Two-hybrid Homo sapiens 40 VENTX   Two-hybrid Homo sapiens 41 ZNF34   Two-hybrid Homo sapiens 42 ITCH 83737 Reconstituted Complex Homo sapiens 43 PHLPP1   Proximity Label-MS Homo sapiens 44 FOXH1   Two-hybrid Homo sapiens 45 SPAG8   Two-hybrid Homo sapiens 46 IGFN1   Two-hybrid Homo sapiens 47 PIP 5304 Affinity Capture-MS Homo sapiens 48 BAG2 9532 Affinity Capture-MS Homo sapiens 49 QPCT 25797 Affinity Capture-MS Homo sapiens 50 CUL3 8452 Affinity Capture-MS Homo sapiens 51 FAM46B   Two-hybrid Homo sapiens 52 CDKL5   Affinity Capture-MS Homo sapiens 53 FUS 2521 Affinity Capture-MS Homo sapiens 54 OTX1   Two-hybrid Homo sapiens 55 TFG 10342 Two-hybrid Homo sapiens 56 SCYL2 55681 Affinity Capture-MS Homo sapiens 57 MYC   Affinity Capture-MS Homo sapiens 58 BAG1 573 Affinity Capture-MS Homo sapiens 59 RHOXF2   Two-hybrid Homo sapiens 60 HSPA1A 3303 Affinity Capture-MS Homo sapiens 61 ATPAF2 91647 Two-hybrid Homo sapiens 62 VDAC1 7416 Co-fractionation Homo sapiens 63 SHC3   Two-hybrid Homo sapiens 64 THY1 7070 Co-fractionation Homo sapiens 65 NPAS2   Two-hybrid Homo sapiens 66 PLEKHA4 57664 Affinity Capture-MS Homo sapiens 67 NUTF2 10204 Two-hybrid Homo sapiens 68 SIRT6   Affinity Capture-MS Homo sapiens 69 PARP1 142 Proximity Label-MS Homo sapiens 70 VPS37C 55048 Two-hybrid Homo sapiens 71 WWOX 51741 Affinity Capture-MS Homo sapiens 72 FXR1 8087 Affinity Capture-MS Homo sapiens 73 JMJD7   Two-hybrid Homo sapiens 74 LCK 3932 Two-hybrid Homo sapiens 75 WRAP53   Two-hybrid Homo sapiens 76 ASB13   Two-hybrid Homo sapiens 77 S100A10 6281 Co-fractionation Homo sapiens 78 PRMT1 3276 Affinity Capture-MS Homo sapiens 79 G3BP2 9908 Affinity Capture-MS Homo sapiens 80 RAVER1 125950 Affinity Capture-MS Homo sapiens 81 RBFOX2 23543 Affinity Capture-MS Homo sapiens 82 NUP50 10762 Co-fractionation Homo sapiens 83 GLUL 2752 Affinity Capture-MS Homo sapiens 84 PINK1   Affinity Capture-MS Homo sapiens 85 KANK2 25959 Two-hybrid Homo sapiens 86 PRSS53   Two-hybrid Homo sapiens 87 SERBP1 26135 Proximity Label-MS Homo sapiens 88 TEP1 7011 Two-hybrid Homo sapiens 89 FLAD1 80308 Affinity Capture-MS Homo sapiens 90 RPA3 6119 Proximity Label-MS Homo sapiens 91 FAM168B 130074 Two-hybrid Homo sapiens View the network

Pathways in which UBAP2 is involved

No pathways found