Gene description for QPCT
Gene name glutaminyl-peptide cyclotransferase
Gene symbol QPCT
Other names/aliases GCT
QC
sQC
Species Homo sapiens
 Database cross references - QPCT
ExoCarta ExoCarta_25797
Vesiclepedia VP_25797
Entrez Gene 25797
HGNC 9753
MIM 607065
UniProt Q16769  
 QPCT identified in exosomes derived from the following tissue/cell type
Colorectal cancer cells 17956143    
Colorectal cancer cells 23161513    
Colorectal cancer cells 23161513    
Colorectal cancer cells 23161513    
Mesenchymal stem cells 36408942    
 Gene ontology annotations for QPCT
Molecular Function
    protein binding GO:0005515 IPI
    zinc ion binding GO:0008270 IBA
    zinc ion binding GO:0008270 IDA
    glutaminyl-peptide cyclotransferase activity GO:0016603 IBA
    glutaminyl-peptide cyclotransferase activity GO:0016603 IDA
Biological Process
    peptidyl-pyroglutamic acid biosynthetic process, using glutaminyl-peptide cyclotransferase GO:0017186 IDA
    protein modification process GO:0036211 TAS
Subcellular Localization
    extracellular region GO:0005576 TAS
    specific granule lumen GO:0035580 TAS
    extracellular exosome GO:0070062 HDA
    tertiary granule lumen GO:1904724 TAS
    ficolin-1-rich granule lumen GO:1904813 TAS
 Experiment description of studies that identified QPCT in exosomes
1
Experiment ID 20
MISEV standards
✔
EM
Biophysical techniques
✔
HSP90|CD63|CD81|LAMP1
Enriched markers
✔
GOLGA2|cytochrome c
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 17956143    
Organism Homo sapiens
Experiment description Proteomic analysis of microvesicles derived from human colorectal cancer cells.
Authors "Choi DS, Lee JM, Park GW, Lim HW, Bang JY, Kim YK, Kwon KH, Kwon HJ, Kim KP, Gho YS"
Journal name JPR
Publication year 2007
Sample Colorectal cancer cells
Sample name HT29
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Diafiltration
Flotation density 1.16 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [LTQ]
Western blotting
2
Experiment ID 207
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|HSP70|FLOT1
Enriched markers
✔
VDAC
Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23161513    
Organism Homo sapiens
Experiment description Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS.
Authors "Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ."
Journal name Mol Cell Proteomics
Publication year 2012
Sample Colorectal cancer cells
Sample name DKO-1
Isolation/purification methods Differential centrifugation
Filtration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
3
Experiment ID 208
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|HSP70|FLOT1
Enriched markers
✔
VDAC
Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23161513    
Organism Homo sapiens
Experiment description Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS.
Authors "Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ."
Journal name Mol Cell Proteomics
Publication year 2012
Sample Colorectal cancer cells
Sample name Dks-8
Isolation/purification methods Differential centrifugation
Filtration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
4
Experiment ID 209
MISEV standards
✘ Biophysical techniques
✔
TSG101|HSP70|FLOT1
Enriched markers
✔
VDAC
Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23161513    
Organism Homo sapiens
Experiment description Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS.
Authors "Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ."
Journal name Mol Cell Proteomics
Publication year 2012
Sample Colorectal cancer cells
Sample name DLD-1
Isolation/purification methods Differential centrifugation
Filtration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
5
Experiment ID 488
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD81|CD63|GAPDH|SDCBP|LAMP1|TFRC|UCHL1|FLOT2|LAMP2|FLOT1|ICAM1|RAB5B|CD151|RAB35|TSG101|RAB5A|CD82
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Homo sapiens
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Mesenchymal stem cells
Sample name UCMSC
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
 Protein-protein interactions for QPCT
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 UBE2H 7328
Affinity Capture-MS Homo sapiens
2 CUL1 8454
Affinity Capture-MS Homo sapiens
3 ARIH2 10425
Affinity Capture-MS Homo sapiens
4 HRAS 3265
Affinity Capture-Western Homo sapiens
Co-localization Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
5 NUBP2 10101
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
6 UBAP2 55833
Affinity Capture-MS Homo sapiens
7 UBQLN2 29978
Two-hybrid Homo sapiens
8 CUL5 8065
Affinity Capture-MS Homo sapiens
9 CUL3 8452
Affinity Capture-MS Homo sapiens
10 CAND1 55832
Affinity Capture-MS Homo sapiens
11 STK38L 23012
Affinity Capture-MS Homo sapiens
12 CMBL 134147
Affinity Capture-MS Homo sapiens
13 CUL4B 8450
Affinity Capture-MS Homo sapiens
14 UBB 7314
Affinity Capture-MS Homo sapiens
15 DCUN1D1 54165
Affinity Capture-MS Homo sapiens
16 MFAP1  
Affinity Capture-MS Homo sapiens
17 SUV420H2  
Affinity Capture-MS Homo sapiens
18 CUL4A 8451
Affinity Capture-MS Homo sapiens
19 DHRS4 10901
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
20 FRAS1 80144
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
21 COPS5 10987
Affinity Capture-MS Homo sapiens
22 NUBP1 4682
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
23 C9orf72  
Affinity Capture-MS Homo sapiens
24 CUL2 8453
Affinity Capture-MS Homo sapiens
25 NEDD8 4738
Affinity Capture-MS Homo sapiens
26 NAP1L5  
Affinity Capture-MS Homo sapiens
27 ELP3 55140
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
View the network image/svg+xml
 Pathways in which QPCT is involved
PathwayEvidenceSource
Immune System TAS Reactome
Innate Immune System TAS Reactome
Neutrophil degranulation TAS Reactome





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