Gene description for NIF3L1
Gene name NIF3 NGG1 interacting factor 3-like 1 (S. cerevisiae)
Gene symbol NIF3L1
Other names/aliases ALS2CR1
CALS-7
MDS015
Species Homo sapiens
 Database cross references - NIF3L1
ExoCarta ExoCarta_60491
Vesiclepedia VP_60491
Entrez Gene 60491
HGNC 13390
MIM 605778
UniProt Q9GZT8  
 NIF3L1 identified in exosomes derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Ovarian cancer cells 23333927    
Ovarian cancer cells 23333927    
 Gene ontology annotations for NIF3L1
Molecular Function
    protein binding GO:0005515 IPI
    identical protein binding GO:0042802 IPI
    RNA polymerase II-specific DNA-binding transcription factor binding GO:0061629 IPI
Biological Process
    negative regulation of transcription by RNA polymerase II GO:0000122 ISS
    neuron differentiation GO:0030182 ISS
    positive regulation of DNA-templated transcription GO:0045893 IDA
Subcellular Localization
    nucleus GO:0005634 ISS
    cytoplasm GO:0005737 IBA
    cytoplasm GO:0005737 IDA
    mitochondrion GO:0005739 IBA
 Experiment description of studies that identified NIF3L1 in exosomes
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 211
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|Alix|EpCAM|TFRC
Enriched markers
✔
cytochrome c|GOLGA2
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors "Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K."
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name IGROV1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
6
Experiment ID 212
MISEV standards
✔
CEM
Biophysical techniques
✔
TSG101|Alix|EpCAM|TFRC
Enriched markers
✔
Cytochrome C|GOLGA2
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors "Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K."
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name OVCAR-3
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for NIF3L1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 ACTR2 10097
Co-fractionation Homo sapiens
2 GNMT 27232
Two-hybrid Homo sapiens
3 DPP9 91039
Co-fractionation Homo sapiens
4 NDRG1 10397
Co-fractionation Homo sapiens
5 ZNF326 284695
Affinity Capture-MS Homo sapiens
6 COIL  
Two-hybrid Homo sapiens
7 STAT5B 6777
Co-fractionation Homo sapiens
8 NPEPL1 79716
Co-fractionation Homo sapiens
9 CCS 9973
Co-fractionation Homo sapiens
10 NOTCH2NL 388677
Affinity Capture-MS Homo sapiens
11 SH3GL1 6455
Co-fractionation Homo sapiens
12 ARPC4 10093
Co-fractionation Homo sapiens
13 LAP3 51056
Co-fractionation Homo sapiens
14 DDX17 10521
Affinity Capture-MS Homo sapiens
15 AP5B1 91056
Two-hybrid Homo sapiens
16 TSG101 7251
Co-fractionation Homo sapiens
17 IP6K3  
Two-hybrid Homo sapiens
18 THOC7 80145
Two-hybrid Homo sapiens
19 CCDC9  
Affinity Capture-MS Homo sapiens
20 ENOX1  
Two-hybrid Homo sapiens
21 KRTAP13-2  
Affinity Capture-MS Homo sapiens
22 CCDC102B  
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
23 APEX1 328
Affinity Capture-RNA Homo sapiens
24 PCBD1 5092
Two-hybrid Homo sapiens
25 LMO2  
Two-hybrid Homo sapiens
26 TSC22D4 81628
Two-hybrid Homo sapiens
27 PPP2R4 5524
Two-hybrid Homo sapiens
28 RBM3 5935
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
29 PPP2R5D 5528
Co-fractionation Homo sapiens
30 NME7 29922
Two-hybrid Homo sapiens
31 ACTR3 10096
Co-fractionation Homo sapiens
32 YES1 7525
Two-hybrid Homo sapiens
33 STAT3 6774
Two-hybrid Homo sapiens
34 ORC5  
Two-hybrid Homo sapiens
35 OXT  
Affinity Capture-MS Homo sapiens
36 NOL3 8996
Two-hybrid Homo sapiens
37 LPP 4026
Co-fractionation Homo sapiens
38 LAMTOR3 8649
Two-hybrid Homo sapiens
39 KRTAP6-1  
Affinity Capture-MS Homo sapiens
40 BDKRB2  
Two-hybrid Homo sapiens
41 SEPT1 1731
Two-hybrid Homo sapiens
42 CHN1  
Co-fractionation Homo sapiens
43 CDKN2D  
Two-hybrid Homo sapiens
44 NIF3L1 60491
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
45 PRNP 5621
Affinity Capture-MS Homo sapiens
46 DMRTB1  
Two-hybrid Homo sapiens
47 RBMX 27316
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
48 KRTAP15-1  
Affinity Capture-MS Homo sapiens
49 SULT2A1  
Two-hybrid Homo sapiens
50 ACP2 53
Co-fractionation Homo sapiens
51 TOM1L1 10040
Co-fractionation Homo sapiens
52 PPIH 10465
Two-hybrid Homo sapiens
53 PFDN5 5204
Co-fractionation Homo sapiens
54 FUBP1 8880
Co-fractionation Homo sapiens
55 SH2D1A 4068
Affinity Capture-MS Homo sapiens
56 TRIM21 6737
Two-hybrid Homo sapiens
57 ARPC2 10109
Co-fractionation Homo sapiens
58 BEND7 222389
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
59 PPP2CA 5515
Co-fractionation Homo sapiens
60 DCTPP1 79077
Two-hybrid Homo sapiens
61 YWHAQ 10971
Affinity Capture-Western Homo sapiens
62 TPRKB 51002
Co-fractionation Homo sapiens
63 FAM49B 51571
Two-hybrid Homo sapiens
64 SAT1  
Two-hybrid Homo sapiens
65 DUSP23 54935
Two-hybrid Homo sapiens
66 METTL1 4234
Co-fractionation Homo sapiens
67 FAM73B  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
68 VPS37B 79720
Co-fractionation Homo sapiens
69 DYNLT3 6990
Two-hybrid Homo sapiens
70 PAPLN  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
71 KCNIP1  
Two-hybrid Homo sapiens
72 TRIP13 9319
Two-hybrid Homo sapiens
73 PALM2  
Co-fractionation Homo sapiens
74 GALT 2592
Affinity Capture-MS Homo sapiens
75 COPS2 9318
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Two-hybrid Homo sapiens
76 UGT1A4  
Affinity Capture-MS Homo sapiens
77 C9orf24  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
78 BAG1 573
Co-fractionation Homo sapiens
79 KCTD9  
Two-hybrid Homo sapiens
80 MAGEA11  
Two-hybrid Homo sapiens
81 DUPD1  
Two-hybrid Homo sapiens
82 C14orf119  
Two-hybrid Homo sapiens
83 NT5DC1 221294
Co-fractionation Homo sapiens
84 SULT1A2  
Two-hybrid Homo sapiens
85 TCL1A  
Two-hybrid Homo sapiens
86 OSGEP 55644
Co-fractionation Homo sapiens
87 TNFRSF18  
Affinity Capture-MS Homo sapiens
88 AGFG1 3267
Co-fractionation Homo sapiens
89 SNF8 11267
Two-hybrid Homo sapiens
90 NATD1  
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
91 SYT17  
Two-hybrid Homo sapiens
92 KRT15 3866
Two-hybrid Homo sapiens
93 KIF1BP 26128
Co-fractionation Homo sapiens
94 RABAC1 10567
Two-hybrid Homo sapiens
95 AVP  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
96 FXR2 9513
Two-hybrid Homo sapiens
97 EIF2D 1939
Co-fractionation Homo sapiens
98 ALKBH4  
Two-hybrid Homo sapiens
99 TACC1 6867
Co-fractionation Homo sapiens
100 CCDC85B  
Two-hybrid Homo sapiens
101 DHPS 1725
Two-hybrid Homo sapiens
102 TRIM27  
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
103 C1orf94  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
104 TIFA  
Two-hybrid Homo sapiens
105 TP53RK 112858
Co-fractionation Homo sapiens
106 FBLIM1 54751
Affinity Capture-MS Homo sapiens
107 CUTC  
Two-hybrid Homo sapiens
108 RPIA 22934
Two-hybrid Homo sapiens
109 DCTD 1635
Two-hybrid Homo sapiens
110 AARS 16
Co-fractionation Homo sapiens
111 ARPIN  
Two-hybrid Homo sapiens
112 UBXN2B  
Affinity Capture-MS Homo sapiens
113 PPP2CB 5516
Co-fractionation Homo sapiens
114 CDKN2C  
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
115 FAM103A1  
Affinity Capture-MS Homo sapiens
116 EPHB6  
Two-hybrid Homo sapiens
117 MEOX1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
118 NAPRT 93100
Co-fractionation Homo sapiens
119 NUDT21 11051
Two-hybrid Homo sapiens
120 PLXNB2 23654
Co-fractionation Homo sapiens
121 TPD52L2 7165
Co-fractionation Homo sapiens
122 STK16 8576
Two-hybrid Homo sapiens
123 TRAF2 7186
Two-hybrid Homo sapiens
124 GRB2 2885
Two-hybrid Homo sapiens
125 VPS28 51160
Co-fractionation Homo sapiens
126 CASP7  
Two-hybrid Homo sapiens
127 NEK6 10783
Two-hybrid Homo sapiens
128 CALU 813
Co-fractionation Homo sapiens
129 POLR1C 9533
Two-hybrid Homo sapiens
130 FLAD1 80308
Two-hybrid Homo sapiens
131 PNPLA1 285848
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
132 KCTD7 154881
Two-hybrid Homo sapiens
133 C9orf64 84267
Affinity Capture-MS Homo sapiens
134 PRTFDC1 56952
Two-hybrid Homo sapiens
135 EIF5A2 56648
Two-hybrid Homo sapiens
136 MAPRE2 10982
Two-hybrid Homo sapiens
137 GRPEL1 80273
Co-fractionation Homo sapiens
138 PNMA1 9240
Two-hybrid Homo sapiens
139 EIF4A3 9775
Two-hybrid Homo sapiens
140 SPACA4  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
141 DDX19A 55308
Co-fractionation Homo sapiens
142 AARSD1 80755
Co-fractionation Homo sapiens
143 GORASP1 64689
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
144 HBQ1  
Affinity Capture-MS Homo sapiens
145 SULT1A3 6818
Two-hybrid Homo sapiens
146 TXN 7295
Two-hybrid Homo sapiens
147 DMC1  
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
148 DCDC2  
Two-hybrid Homo sapiens
149 EIF4B 1975
Affinity Capture-MS Homo sapiens
150 SH3GLB1 51100
Co-fractionation Homo sapiens
151 KXD1 79036
Two-hybrid Homo sapiens
152 RPRD1B 58490
Co-fractionation Homo sapiens
153 KCTD17 79734
Two-hybrid Homo sapiens
154 Mlxipl  
Affinity Capture-Western Mus musculus
155 S100A1 6271
Two-hybrid Homo sapiens
156 CDKN2B 1030
Two-hybrid Homo sapiens
157 NUDT14 256281
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
158 ZBED1  
Two-hybrid Homo sapiens
159 VIM 7431
Two-hybrid Homo sapiens
160 ODF1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
161 NGB  
Two-hybrid Homo sapiens
162 RPA3 6119
Co-fractionation Homo sapiens
Proximity Label-MS Homo sapiens
163 RANGAP1 5905
Co-fractionation Homo sapiens
164 SSX2IP  
Two-hybrid Homo sapiens
View the network image/svg+xml
 Pathways in which NIF3L1 is involved
No pathways found





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