Gene description for NIF3L1
Gene name NIF3 NGG1 interacting factor 3-like 1 (S. cerevisiae)
Gene symbol NIF3L1
Other names/aliases ALS2CR1
CALS-7
MDS015
Species Homo sapiens
 Database cross references - NIF3L1
ExoCarta ExoCarta_60491
Vesiclepedia VP_60491
Entrez Gene 60491
HGNC 13390
MIM 605778
UniProt Q9GZT8  
 NIF3L1 identified in exosomes derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Ovarian cancer cells 23333927    
Ovarian cancer cells 23333927    
 Gene ontology annotations for NIF3L1
Molecular Function
    transcription factor binding GO:0008134 IPI
    protein binding GO:0005515 IPI
    identical protein binding GO:0042802 IPI
Biological Process
    negative regulation of nucleic acid-templated transcription GO:1903507 ISS
    neuron differentiation GO:0030182 ISS
    positive regulation of transcription, DNA-templated GO:0045893 IDA
Subcellular Localization
    nucleus GO:0005634 ISS
    cytoplasm GO:0005737 IDA
    mitochondrion GO:0005739 IBA
 Experiment description of studies that identified NIF3L1 in exosomes
1
Experiment ID 489
ISEV standards
✔
EM
EV Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
EV Enriched markers
✔
Canx
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ.
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
ISEV standards
✔
EM
EV Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
EV Enriched markers
✔
Canx
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ.
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
ISEV standards
✔
EM
EV Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
EV Enriched markers
✔
Canx
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ.
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
ISEV standards
✔
EM
EV Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
EV Enriched markers
✔
Canx
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ.
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 211
ISEV standards
✔
EM
EV Biophysical techniques
✔
TSG101|Alix|EpCAM|TFRC
EV Enriched markers
✔
cytochrome c|GOLGA2
EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K.
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name IGROV1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
6
Experiment ID 212
ISEV standards
✔
CEM
EV Biophysical techniques
✔
TSG101|Alix|EpCAM|TFRC
EV Enriched markers
✔
Cytochrome C|GOLGA2
EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K.
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name OVCAR-3
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for NIF3L1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 TRIP13 9319
Two-hybrid Homo sapiens
2 NUDT21 11051
Two-hybrid Homo sapiens
3 GNMT 27232
Two-hybrid Homo sapiens
4 TRAF2 7186
Two-hybrid Homo sapiens
5 COPS2 9318
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Two-hybrid Homo sapiens
6 NIF3L1 60491
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
7 FLJ10094  
Two-hybrid Homo sapiens
8 DMRTB1  
Two-hybrid Homo sapiens
9 MAGEA11  
Two-hybrid Homo sapiens
10 PRTFDC1 56952
Two-hybrid Homo sapiens
11 MAPRE2 10982
Two-hybrid Homo sapiens
12 THOC7 80145
Two-hybrid Homo sapiens
13 SNF8 11267
Two-hybrid Homo sapiens
14 Wbscr14  
Affinity Capture-Western Mus musculus
15 TIFA  
Two-hybrid Homo sapiens
16 PCBD1 5092
Two-hybrid Homo sapiens
17 KRT15 3866
Two-hybrid Homo sapiens
18 TSC22D4 81628
Two-hybrid Homo sapiens
19 RABAC1 10567
Two-hybrid Homo sapiens
20 TRIM21 6737
Two-hybrid Homo sapiens
21 DHPS 1725
Two-hybrid Homo sapiens
22 KXD1 79036
Two-hybrid Homo sapiens
23 BEND7 222389
Two-hybrid Homo sapiens
24 DIPA  
Two-hybrid Homo sapiens
25 KCTD17 79734
Two-hybrid Homo sapiens
26 YWHAQ 10971
Affinity Capture-Western Homo sapiens
27 CUTC  
Two-hybrid Homo sapiens
28 RPIA 22934
Two-hybrid Homo sapiens
29 S100A1 6271
Two-hybrid Homo sapiens
30 VIM 7431
Two-hybrid Homo sapiens
31 NOL3 8996
Two-hybrid Homo sapiens
32 DCTD 1635
Two-hybrid Homo sapiens
33 DCTPP1 79077
Two-hybrid Homo sapiens
34 ZBED1  
Two-hybrid Homo sapiens
View the network image/svg+xml
 Pathways in which NIF3L1 is involved
No pathways found





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