Gene description for EIF2D
Gene name eukaryotic translation initiation factor 2D
Gene symbol EIF2D
Other names/aliases HCA56
LGTN
Species Homo sapiens
 Database cross references - EIF2D
ExoCarta ExoCarta_1939
Vesiclepedia VP_1939
Entrez Gene 1939
HGNC 6583
MIM 613709
UniProt P41214  
 EIF2D identified in sEVs derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Mesenchymal stem cells 36408942    
Thymus 23844026    
 Gene ontology annotations for EIF2D
Molecular Function
    RNA binding GO:0003723 IEA
    translation initiation factor activity GO:0003743 IBA
    translation initiation factor activity GO:0003743 IDA
    signaling receptor activity GO:0038023 TAS
Biological Process
    formation of translation preinitiation complex GO:0001731 IBA
    formation of translation preinitiation complex GO:0001731 IDA
    intracellular protein transport GO:0006886 TAS
    ribosome disassembly GO:0032790 IDA
    IRES-dependent viral translational initiation GO:0075522 IDA
Subcellular Localization
    cytoplasm GO:0005737 IDA
    cytosol GO:0005829 IDA
    nuclear body GO:0016604 IDA
 Experiment description of studies that identified EIF2D in sEVs
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 488
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD81|CD63|GAPDH|SDCBP|LAMP1|TFRC|UCHL1|FLOT2|LAMP2|FLOT1|ICAM1|RAB5B|CD151|RAB35|TSG101|RAB5A|CD82
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Homo sapiens
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Mesenchymal stem cells
Sample name UCMSC
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
6
Experiment ID 217
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|CD81|CD9|CD63
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23844026    
Organism Homo sapiens
Experiment description Characterization of human thymic exosomes.
Authors "Skogberg G, Gudmundsdottir J, van der Post S, Sandstrom K, Bruhn S, Benson M, Mincheva-Nilsson L, Baranov V, Telemo E, Ekwall O."
Journal name PLoS One
Publication year 2013
Sample Thymus
Sample name Normal-Thymus
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for EIF2D
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 ATG16L1 55054
Affinity Capture-MS Homo sapiens
2 DENR 8562
Co-fractionation Homo sapiens
3 DHX15 1665
Co-fractionation Homo sapiens
4 RECQL5  
Co-fractionation Homo sapiens
5 EIF3G 8666
Affinity Capture-MS Homo sapiens
6 FASN 2194
Positive Genetic Homo sapiens
7 WDR5 11091
Co-fractionation Homo sapiens
8 GMPPB 29925
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
9 NIF3L1 60491
Co-fractionation Homo sapiens
10 PSMC3 5702
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
11 NAA38  
Co-fractionation Homo sapiens
12 RPS2 6187
Co-fractionation Homo sapiens
13 EIF1B  
Co-fractionation Homo sapiens
Co-fractionation Homo sapiens
14 KIN  
Co-fractionation Homo sapiens
15 ZNHIT2 741
Co-fractionation Homo sapiens
16 RPS12 6206
Co-fractionation Homo sapiens
17 CUL3 8452
Affinity Capture-MS Homo sapiens
18 CSNK2A1 1457
Affinity Capture-MS Homo sapiens
19 MANF 7873
Co-fractionation Homo sapiens
20 ELAC2 60528
Co-fractionation Homo sapiens
21 HNRNPR 10236
Co-fractionation Homo sapiens
22 EIF1 10209
Co-fractionation Homo sapiens
Co-fractionation Homo sapiens
23 GCN1L1 10985
Co-fractionation Homo sapiens
24 PRPS2 5634
Affinity Capture-MS Homo sapiens
25 RCC2 55920
Co-fractionation Homo sapiens
26 RPS28 6234
Co-fractionation Homo sapiens
27 DUSP12 11266
Affinity Capture-MS Homo sapiens
28 CALU 813
Co-fractionation Homo sapiens
29 PCBP1 5093
Affinity Capture-MS Homo sapiens
30 PSPC1 55269
Affinity Capture-MS Homo sapiens
31 C11orf87  
Affinity Capture-MS Homo sapiens
32 DNAJC18  
Affinity Capture-MS Homo sapiens
33 CKAP5 9793
Co-fractionation Homo sapiens
34 HARS 3035
Co-fractionation Homo sapiens
Co-fractionation Homo sapiens
35 SNRPG 6637
Cross-Linking-MS (XL-MS) Homo sapiens
36 BICD2 23299
Proximity Label-MS Homo sapiens
37 RPS11 6205
Affinity Capture-MS Homo sapiens
38 MCTS1 28985
Co-fractionation Homo sapiens
39 GNB2L1 10399
Affinity Capture-MS Homo sapiens
40 SLC22A4 6583
Affinity Capture-MS Homo sapiens
41 MKI67  
Affinity Capture-MS Homo sapiens
42 RAD51  
Affinity Capture-MS Homo sapiens
43 LARP4 113251
Co-fractionation Homo sapiens
44 CDC5L 988
Affinity Capture-MS Homo sapiens
45 RPS16 6217
Affinity Capture-MS Homo sapiens
View the network image/svg+xml
 Pathways in which EIF2D is involved
No pathways found





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