Gene description for CDKN2B
Gene name cyclin-dependent kinase inhibitor 2B (p15, inhibits CDK4)
Gene symbol CDKN2B
Other names/aliases CDK4I
INK4B
MTS2
P15
TP15
p15INK4b
Species Homo sapiens
 Database cross references - CDKN2B
ExoCarta ExoCarta_1030
Vesiclepedia VP_1030
Entrez Gene 1030
HGNC 1788
MIM 600431
UniProt P42772  
 CDKN2B identified in exosomes derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Hepatocellular carcinoma cells 26054723    
 Gene ontology annotations for CDKN2B
Molecular Function
    cyclin-dependent protein serine/threonine kinase inhibitor activity GO:0004861 NAS
    protein kinase binding GO:0019901 IPI
    protein binding GO:0005515 IPI
Biological Process
    transforming growth factor beta receptor signaling pathway GO:0007179 TAS
    negative regulation of cell proliferation GO:0008285 IMP
    regulation of cyclin-dependent protein serine/threonine kinase activity GO:0000079 IDA
    cellular response to nutrient GO:0031670 IMP
    mitotic cell cycle GO:0000278 TAS
    positive regulation of epithelial cell differentiation GO:0030858 IEA
    cell cycle arrest GO:0007050 IMP
    cellular response to extracellular stimulus GO:0031668 IMP
    positive regulation of transcription from RNA polymerase II promoter GO:0045944 TAS
    G2/M transition of mitotic cell cycle GO:0000086 IMP
    transcription, DNA-templated GO:0006351 TAS
    response to cytokine GO:0034097 IEA
    positive regulation of transforming growth factor beta receptor signaling pathway GO:0030511 IMP
    negative regulation of phosphorylation GO:0042326 IDA
    negative regulation of G1/S transition of mitotic cell cycle GO:2000134 IEA
    transcription initiation from RNA polymerase II promoter GO:0006367 TAS
    response to organic cyclic compound GO:0014070 IEA
    aging GO:0007568 IEA
    mitotic cell cycle checkpoint GO:0007093 IMP
    gene expression GO:0010467 TAS
    negative regulation of protein serine/threonine kinase activity GO:0071901 NAS
    megakaryocyte differentiation GO:0030219 IEP
    liver development GO:0001889 IEA
    negative regulation of epithelial cell proliferation GO:0050680 IMP
    spleen development GO:0048536 IEA
Subcellular Localization
    cytosol GO:0005829 TAS
    nucleus GO:0005634 IDA
    nucleoplasm GO:0005654 IDA
    cytoplasm GO:0005737 IDA
 Experiment description of studies that identified CDKN2B in exosomes
1
Experiment ID 489
MISEV standards
✔
EM
EV Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
EV Enriched markers
✔
Canx
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ.
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
EV Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
EV Enriched markers
✔
Canx
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ.
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
EV Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
EV Enriched markers
✔
Canx
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ.
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
EV Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
EV Enriched markers
✔
Canx
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ.
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 235
MISEV standards
✔
EM
EV Biophysical techniques
✔
TSG101|Alix|HSC70|GAPDH
EV Enriched markers
✔
HSP90B1
EV Negative markers
✔
qNano
EV Particle analysis
Identified molecule mRNA
Identification method RNA Sequencing
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocellular carcinoma cells
Sample name HKCI-8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RT-PCR
RNA Sequencing
 Protein-protein interactions for CDKN2B
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 ARPC3 10094
Two-hybrid Homo sapiens
2 IKBKAP 8518
Two-hybrid Homo sapiens
3 MDS025  
Two-hybrid Homo sapiens
4 CDK4 1019
Invivo Homo sapiens
Invivo Homo sapiens
Invitro Homo sapiens
Invitro Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
Two-hybrid Homo sapiens
5 KIAA1377  
Two-hybrid Homo sapiens
6 CDK8 1024
Invitro Homo sapiens
7 MAGEA11  
Two-hybrid Homo sapiens
8 CDK2AP1  
Affinity Capture-MS Homo sapiens
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