Gene description for CDKN2B
Gene name cyclin-dependent kinase inhibitor 2B (p15, inhibits CDK4)
Gene symbol CDKN2B
Other names/aliases CDK4I
INK4B
MTS2
P15
TP15
p15INK4b
Species Homo sapiens
 Database cross references - CDKN2B
ExoCarta ExoCarta_1030
Vesiclepedia VP_1030
Entrez Gene 1030
HGNC 1788
MIM 600431
UniProt P42772  
 CDKN2B identified in sEVs derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Hepatocellular carcinoma cells 26054723    
 Gene ontology annotations for CDKN2B
Molecular Function
    cyclin-dependent protein serine/threonine kinase inhibitor activity GO:0004861 IBA
    cyclin-dependent protein serine/threonine kinase inhibitor activity GO:0004861 IDA
    cyclin-dependent protein serine/threonine kinase inhibitor activity GO:0004861 NAS
    protein binding GO:0005515 IPI
    protein kinase binding GO:0019901 IPI
Biological Process
    regulation of cyclin-dependent protein serine/threonine kinase activity GO:0000079 IDA
    liver development GO:0001889 IEA
    negative regulation of cell population proliferation GO:0008285 IBA
    negative regulation of cell population proliferation GO:0008285 IMP
    response to organic cyclic compound GO:0014070 IEA
    megakaryocyte differentiation GO:0030219 IEP
    positive regulation of transforming growth factor beta receptor signaling pathway GO:0030511 IMP
    positive regulation of epithelial cell differentiation GO:0030858 IEA
    cellular response to nutrient GO:0031670 IMP
    response to cytokine GO:0034097 IEA
    negative regulation of phosphorylation GO:0042326 IDA
    spleen development GO:0048536 IEA
    negative regulation of epithelial cell proliferation GO:0050680 IMP
    negative regulation of glial cell proliferation GO:0060253 IEA
    regulation of G0 to G1 transition GO:0070316 IMP
    cellular response to cell-matrix adhesion GO:0071460 IMP
    cellular response to transforming growth factor beta stimulus GO:0071560 IEA
    cellular senescence GO:0090398 IMP
    regulation of G1/S transition of mitotic cell cycle GO:2000045 IBA
    negative regulation of G1/S transition of mitotic cell cycle GO:2000134 IEA
Subcellular Localization
    nucleus GO:0005634 IBA
    nucleus GO:0005634 IDA
    cytoplasm GO:0005737 IBA
    cytoplasm GO:0005737 IDA
    cytosol GO:0005829 TAS
 Experiment description of studies that identified CDKN2B in sEVs
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 235
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|Alix|HSC70|GAPDH
Enriched markers
✔
HSP90B1
Negative markers
✔
qNano
Particle analysis
Identified molecule mRNA
Identification method RNA Sequencing
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors "He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N"
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocellular carcinoma cells
Sample name HKCI-8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RT-PCR
RNA Sequencing
 Protein-protein interactions for CDKN2B
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 ARPC3 10094
Two-hybrid Homo sapiens
2 IKBKAP 8518
Two-hybrid Homo sapiens
3 AK2 204
Affinity Capture-MS Homo sapiens
4 NIF3L1 60491
Two-hybrid Homo sapiens
5 CCNC  
Two-hybrid Homo sapiens
6 MAGEA11  
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
7 MAPK1 5594
Affinity Capture-MS Homo sapiens
8 INCA1  
Two-hybrid Homo sapiens
9 CDC37 11140
Affinity Capture-MS Homo sapiens
10 TGFB1I1 7041
Two-hybrid Homo sapiens
11 CDK6 1021
Affinity Capture-Western Homo sapiens
FRET Homo sapiens
Affinity Capture-MS Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
FRET Homo sapiens
12 CNKSR3 154043
Two-hybrid Homo sapiens
13 CCDC33 80125
Two-hybrid Homo sapiens
14 APP 351
Reconstituted Complex Homo sapiens
15 CCDC90B  
Two-hybrid Homo sapiens
16 KIAA1377  
Two-hybrid Homo sapiens
17 CDK4 1019
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
FRET Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
18 RUSC1  
Two-hybrid Homo sapiens
19 PIAS2  
Co-localization Homo sapiens
20 PYCRL 65263
Two-hybrid Homo sapiens
21 CDKN2D  
Affinity Capture-MS Homo sapiens
22 FAM127A  
Two-hybrid Homo sapiens
23 ISL1  
Two-hybrid Homo sapiens
24 CDKN2C  
Affinity Capture-MS Homo sapiens
25 CDKN2A 1029
Affinity Capture-MS Homo sapiens
26 TRAF1 7185
Two-hybrid Homo sapiens
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