ExoCarta: Gene summary

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Gene description for KCTD7
Gene name	potassium channel tetramerization domain containing 7
Gene symbol	KCTD7
Other names/aliases	CLN14 EPM3
Species	Homo sapiens
Database cross references - KCTD7
ExoCarta	ExoCarta_154881
Vesiclepedia	VP_154881
Entrez Gene	154881
HGNC	21957
MIM	611725
UniProt	Q96MP8
KCTD7 identified in exosomes derived from the following tissue/cell type
Bone marrow mesenchymal stem cells	36408942
Bone marrow mesenchymal stem cells	36408942
Bone marrow mesenchymal stem cells	36408942
Bone marrow mesenchymal stem cells	36408942

Gene ontology annotations for KCTD7

Molecular Function
	protein binding	GO:0005515	IPI
	identical protein binding	GO:0042802	IEA
	identical protein binding	GO:0042802	IPI
Biological Process
	intracellular potassium ion homeostasis	GO:0030007	IEA
	protein homooligomerization	GO:0051260	IEA
	membrane hyperpolarization	GO:0060081	IBA
	intracellular glutamate homeostasis	GO:0090461	IMP
Subcellular Localization
	cytoplasm	GO:0005737	IDA
	cytosol	GO:0005829	TAS
	plasma membrane	GO:0005886	IBA
	plasma membrane	GO:0005886	IDA

Experiment description of studies that identified KCTD7 in exosomes

Experiment ID

489

MISEV standards
✔ EM	Biophysical techniques
✔ Cd9\|Cd81\|Cd63\|Gapdh\|Sdcbp\|Lamp1\|Aqp1\|Rab5a\|Icam1\|Cd82\|Itga2b\|Tsg101\|Lamp2\|Rab35\|Flot1\|Flot2\|Cd151\|Rab5b\|Tfrc\|Uchl1	Enriched markers
✔ Canx	Negative markers
✔ NTA	Particle analysis

Identified molecule

Protein

Identification method

Mass spectrometry

PubMed ID

36408942

Organism

Rattus norvegicus

Experiment description

Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells

Authors

Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ.

Journal name

Proteomics

Publication year

2023

Sample

Bone marrow mesenchymal stem cells

Sample name

BMSC - Passage 6

Isolation/purification methods

Differential centrifugation
Filtration
Ultracentrifugation

Flotation density

Molecules identified in the study

Protein

Methods used in the study

Western blotting
Mass spectometry

Experiment ID

490

MISEV standards
✔ EM	Biophysical techniques
✔ Cd9\|Cd81\|Cd63\|Gapdh\|Sdcbp\|Lamp1\|Aqp1\|Rab5a\|Icam1\|Cd82\|Itga2b\|Tsg101\|Lamp2\|Rab35\|Flot1\|Flot2\|Cd151\|Rab5b\|Tfrc\|Uchl1	Enriched markers
✔ Canx	Negative markers
✔ NTA	Particle analysis

Identified molecule

Protein

Identification method

Mass spectrometry

PubMed ID

36408942

Organism

Rattus norvegicus

Experiment description

Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells

Authors

Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ.

Journal name

Proteomics

Publication year

2023

Sample

Bone marrow mesenchymal stem cells

Sample name

BMSC - Passage 7

Isolation/purification methods

Differential centrifugation
Filtration
Ultracentrifugation

Flotation density

Molecules identified in the study

Protein

Methods used in the study

Western blotting
Mass spectometry

Experiment ID

491

MISEV standards
✔ EM	Biophysical techniques
✔ Cd9\|Cd81\|Cd63\|Gapdh\|Sdcbp\|Lamp1\|Aqp1\|Rab5a\|Icam1\|Cd82\|Itga2b\|Tsg101\|Lamp2\|Rab35\|Flot1\|Flot2\|Cd151\|Rab5b\|Tfrc\|Uchl1	Enriched markers
✔ Canx	Negative markers
✔ NTA	Particle analysis

Identified molecule

Protein

Identification method

Mass spectrometry

PubMed ID

36408942

Organism

Rattus norvegicus

Experiment description

Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells

Authors

Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ.

Journal name

Proteomics

Publication year

2023

Sample

Bone marrow mesenchymal stem cells

Sample name

BMSC - Passage 8

Isolation/purification methods

Differential centrifugation
Filtration
Ultracentrifugation

Flotation density

Molecules identified in the study

Protein

Methods used in the study

Western blotting
Mass spectometry

Experiment ID

492

MISEV standards
✔ EM	Biophysical techniques
✔ Cd9\|Cd81\|Cd63\|Gapdh\|Sdcbp\|Lamp1\|Aqp1\|Rab5a\|Icam1\|Cd82\|Itga2b\|Tsg101\|Lamp2\|Rab35\|Flot1\|Flot2\|Cd151\|Rab5b\|Tfrc\|Uchl1	Enriched markers
✔ Canx	Negative markers
✔ NTA	Particle analysis

Identified molecule

Protein

Identification method

Mass spectrometry

PubMed ID

36408942

Organism

Rattus norvegicus

Experiment description

Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells

Authors

Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ.

Journal name

Proteomics

Publication year

2023

Sample

Bone marrow mesenchymal stem cells

Sample name

BMSC - Passage 9

Isolation/purification methods

Differential centrifugation
Filtration
Ultracentrifugation

Flotation density

Molecules identified in the study

Protein

Methods used in the study

Western blotting
Mass spectometry

Protein-protein interactions for KCTD7

Protein Interactor

ExoCarta ID

Identification method

PubMed

Species

HNRNPH1

3187

Affinity Capture-RNA

Homo sapiens

SHKBP1

Affinity Capture-MS

Homo sapiens

TRIM2

Two-hybrid

Homo sapiens

TRIM3

10612

Two-hybrid

Homo sapiens

EFHC2

Two-hybrid

Homo sapiens

NIF3L1

60491

Two-hybrid

Homo sapiens

MDFI

Two-hybrid

Homo sapiens

ZNF438

Two-hybrid

Homo sapiens

PARVG

64098

Two-hybrid

Homo sapiens

ZNF552

Two-hybrid

Homo sapiens

ATXN7L2

Two-hybrid

Homo sapiens

RAB4A

5867

Two-hybrid

Homo sapiens

ADAMTSL4

Two-hybrid

Homo sapiens

PIN1

5300

Two-hybrid

Homo sapiens

COPS6

10980

Affinity Capture-MS

Homo sapiens

REL

5966

Two-hybrid

Homo sapiens

INCA1

Two-hybrid

Homo sapiens

VEZF1

Two-hybrid

Homo sapiens

CUL3

8452

Affinity Capture-Western		Homo sapiens
Affinity Capture-MS		Homo sapiens

UBC

7316

Reconstituted Complex

Homo sapiens

Rabep2

Affinity Capture-MS

Mus musculus

DVL3

1857

Two-hybrid

Homo sapiens

TCEA2

Two-hybrid

Homo sapiens

PSMA1

5682

Two-hybrid

Homo sapiens

NXF1

10482

Two-hybrid

Homo sapiens

Rabep1

Affinity Capture-MS

Mus musculus

KCTD7

154881

Two-hybrid		Homo sapiens
Two-hybrid		Homo sapiens

PRR22

Two-hybrid

Homo sapiens

TERF1

7013

Two-hybrid

Homo sapiens

WDYHV1

Two-hybrid

Homo sapiens

PPARA

Two-hybrid

Homo sapiens

INO80B

83444

Two-hybrid

Homo sapiens

GORASP2

26003

Two-hybrid

Homo sapiens

KRT75

9119

Two-hybrid

Homo sapiens

KAT5

Two-hybrid

Homo sapiens

MEOX2

Two-hybrid

Homo sapiens

ZNF587

Two-hybrid

Homo sapiens

GAS2L2

Two-hybrid

Homo sapiens

PRPF18

Two-hybrid

Homo sapiens

KRTAP13-3

Two-hybrid

Homo sapiens

TCEANC

Two-hybrid

Homo sapiens

KCTD3

Affinity Capture-MS

Homo sapiens

ZNF474

Two-hybrid

Homo sapiens

ZNF417

Two-hybrid

Homo sapiens

BAG4

Two-hybrid

Homo sapiens

View the network

Pathways in which KCTD7 is involved

Pathway	Evidence	Source
Adaptive Immune System	TAS	Reactome
Antigen processing: Ubiquitination & Proteasome degradation	TAS	Reactome
Class I MHC mediated antigen processing & presentation	TAS	Reactome
Immune System	TAS	Reactome
Metabolism of proteins	TAS	Reactome
Neddylation	TAS	Reactome
Post-translational protein modification	TAS	Reactome

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