Experiment description of studies that identified DCTPP1 in exosomes

1 Experiment ID 412 MISEV standards ✔ EM EV Biophysical techniques ✔ CD81|CD9|CD63|SDCBP|LAMP1|GAPDH|FLOT1|TFRC|FLOT2|TSG101|RAB35 EV Enriched markers ✔ CANX|ACTB EV Negative markers ✔ NTA EV Particle analysis Identified molecule Protein Identification method Mass spectrometry PubMed ID 34108659     Organism Homo sapiens Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker Authors Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R. Journal name Nat Cell Biol Publication year 2021 Sample Breast cancer cells Sample name MCF7 Isolation/purification methods Differential centrifugation Filtration Ultracentrifugation Flotation density - Molecules identified in the study Protein mRNA Methods used in the study RT-qPCR Western blotting Mass spectrometry Flow cytometry

2 Experiment ID 414 MISEV standards ✔ EM EV Biophysical techniques ✔ CD81|CD9|CD63|SDCBP|LAMP1|GAPDH|FLOT1|TFRC|FLOT2|TSG101|RAB35 EV Enriched markers ✔ CANX EV Negative markers ✔ NTA EV Particle analysis Identified molecule Protein Identification method Mass spectrometry PubMed ID 34108659     Organism Homo sapiens Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker Authors Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R. Journal name Nat Cell Biol Publication year 2021 Sample Breast cancer cells Sample name MDA-MB-231 Isolation/purification methods Differential centrifugation Filtration Ultracentrifugation Flotation density - Molecules identified in the study Protein mRNA Methods used in the study RT-qPCR Western blotting Mass spectrometry Flow cytometry

3 Experiment ID 426 MISEV standards ✘ EV Biophysical techniques ✔ SDCBP|FLOT1|CD81|CD9|CD151|FLOT2|TSG101|LAMP1|TFRC|RAB5A|RAB35|GAPDH|UCHL1|ICAM1 EV Enriched markers ✔ CANX EV Negative markers ✘ EV Particle analysis Identified molecule Protein Identification method Mass spectrometry PubMed ID 34108659     Organism Homo sapiens Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker Authors Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R. Journal name Nat Cell Biol Publication year 2021 Sample Breast cancer cells Sample name MDA-MB-231 - Exo-rich fractions 7-10 pooled Isolation/purification methods Differential centrifugation Filtration Ultracentrifugation Size exclusion chromatography Flotation density - Molecules identified in the study Protein Methods used in the study Western blotting Mass spectrometry

4 Experiment ID 427 MISEV standards ✘ EV Biophysical techniques ✔ CD81|SDCBP|FLOT1|CD9|CD151|FLOT2|TSG101|LAMP1|TFRC|RAB5A|RAB35|GAPDH|UCHL1|ICAM1 EV Enriched markers ✔ CANX EV Negative markers ✘ EV Particle analysis Identified molecule Protein Identification method Mass spectrometry PubMed ID 34108659     Organism Homo sapiens Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker Authors Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R. Journal name Nat Cell Biol Publication year 2021 Sample Breast cancer cells Sample name MDA-MB-231 - Exo-rich fractions 1-6 pooled Isolation/purification methods Differential centrifugation Filtration Ultracentrifugation OptiPrep density gradient centrifugation Flotation density - Molecules identified in the study Protein Methods used in the study Mass spectrometry

5 Experiment ID 207 MISEV standards ✔ EM EV Biophysical techniques ✔ TSG101|HSP70|FLOT1 EV Enriched markers ✔ VDAC EV Negative markers ✔ NTA EV Particle analysis Identified molecule protein Identification method Mass spectrometry PubMed ID 23161513     Organism Homo sapiens Experiment description Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS. Authors Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ. Journal name Mol Cell Proteomics Publication year 2012 Sample Colorectal cancer cells Sample name DKO-1 Isolation/purification methods Differential centrifugation Filtration Flotation density - Molecules identified in the study Protein Methods used in the study Mass spectrometry

6 Experiment ID 208 MISEV standards ✔ EM EV Biophysical techniques ✔ TSG101|HSP70|FLOT1 EV Enriched markers ✔ VDAC EV Negative markers ✔ NTA EV Particle analysis Identified molecule protein Identification method Mass spectrometry PubMed ID 23161513     Organism Homo sapiens Experiment description Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS. Authors Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ. Journal name Mol Cell Proteomics Publication year 2012 Sample Colorectal cancer cells Sample name Dks-8 Isolation/purification methods Differential centrifugation Filtration Flotation density - Molecules identified in the study Protein Methods used in the study Mass spectrometry

7 Experiment ID 209 MISEV standards ✘ EV Biophysical techniques ✔ TSG101|HSP70|FLOT1 EV Enriched markers ✔ VDAC EV Negative markers ✔ NTA EV Particle analysis Identified molecule protein Identification method Mass spectrometry PubMed ID 23161513     Organism Homo sapiens Experiment description Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS. Authors Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ. Journal name Mol Cell Proteomics Publication year 2012 Sample Colorectal cancer cells Sample name DLD-1 Isolation/purification methods Differential centrifugation Filtration Flotation density - Molecules identified in the study Protein Methods used in the study Mass spectrometry

8 Experiment ID 407 MISEV standards ✔ EM EV Biophysical techniques ✔ CD9|CD63|CD81|SDCBP|TSG101|LAMP1|GAPDH|FLOT1|TFRC|FLOT2|RAB35 EV Enriched markers ✔ CANX EV Negative markers ✔ NTA EV Particle analysis Identified molecule Protein Identification method Mass spectrometry PubMed ID 34108659     Organism Homo sapiens Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker Authors Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R. Journal name Nat Cell Biol Publication year 2021 Sample Embryonic kidney cells Sample name HEK293T Isolation/purification methods Differential centrifugation Filtration Ultracentrifugation Flotation density - Molecules identified in the study Protein mRNA Methods used in the study RT-qPCR Western blotting Mass spectrometry Flow cytometry

9 Experiment ID 419 MISEV standards ✘ EV Biophysical techniques ✔ SDCBP|FLOT1|CD81|CD9|CD151|FLOT2|TSG101|LAMP1|TFRC|RAB5A|RAB35|GAPDH|UCHL1|ICAM1 EV Enriched markers ✔ CANX EV Negative markers ✘ EV Particle analysis Identified molecule Protein Identification method Mass spectrometry PubMed ID 34108659     Organism Homo sapiens Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker Authors Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R. Journal name Nat Cell Biol Publication year 2021 Sample Embryonic kidney cells Sample name HEK293T - Exo-rich fractions 7-10 pooled Isolation/purification methods Differential centrifugation Filtration Ultracentrifugation Size exclusion chromatography Flotation density - Molecules identified in the study Protein Methods used in the study Western blotting Mass spectrometry

10 Experiment ID 419 MISEV standards ✘ EV Biophysical techniques ✔ SDCBP|FLOT1|CD81|CD9|CD151|FLOT2|TSG101|LAMP1|TFRC|RAB5A|RAB35|GAPDH|UCHL1|ICAM1 EV Enriched markers ✔ CANX EV Negative markers ✘ EV Particle analysis Identified molecule Protein Identification method Mass spectrometry PubMed ID 34108659     Organism Homo sapiens Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker Authors Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R. Journal name Nat Cell Biol Publication year 2021 Sample Embryonic kidney cells Sample name HEK293T - Exo-rich fractions 7-10 pooled Isolation/purification methods Differential centrifugation Filtration Ultracentrifugation Size exclusion chromatography Flotation density - Molecules identified in the study Protein Methods used in the study Western blotting Mass spectrometry

11 Experiment ID 420 MISEV standards ✘ EV Biophysical techniques ✔ CD81|SDCBP|FLOT1|CD9|CD151|FLOT2|TSG101|LAMP1|TFRC|RAB5A|RAB35|GAPDH|UCHL1|ICAM1 EV Enriched markers ✔ CANX EV Negative markers ✘ EV Particle analysis Identified molecule Protein Identification method Mass spectrometry PubMed ID 34108659     Organism Homo sapiens Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker Authors Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R. Journal name Nat Cell Biol Publication year 2021 Sample Embryonic kidney cells Sample name HEK293T - Exo-rich fractions 1-6 pooled Isolation/purification methods Differential centrifugation Filtration Ultracentrifugation OptiPrep density gradient centrifugation Flotation density - Molecules identified in the study Protein Methods used in the study Mass spectrometry

12 Experiment ID 420 MISEV standards ✘ EV Biophysical techniques ✔ CD81|SDCBP|FLOT1|CD9|CD151|FLOT2|TSG101|LAMP1|TFRC|RAB5A|RAB35|GAPDH|UCHL1|ICAM1 EV Enriched markers ✔ CANX EV Negative markers ✘ EV Particle analysis Identified molecule Protein Identification method Mass spectrometry PubMed ID 34108659     Organism Homo sapiens Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker Authors Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R. Journal name Nat Cell Biol Publication year 2021 Sample Embryonic kidney cells Sample name HEK293T - Exo-rich fractions 1-6 pooled Isolation/purification methods Differential centrifugation Filtration Ultracentrifugation OptiPrep density gradient centrifugation Flotation density - Molecules identified in the study Protein Methods used in the study Mass spectrometry

13 Experiment ID 405 MISEV standards ✔ EM EV Biophysical techniques ✔ CD9|CD63|CD81|SDCBP|LAMP1|GAPDH|FLOT1|TFRC|FLOT2|TSG101|RAB35 EV Enriched markers ✔ CANX EV Negative markers ✔ NTA EV Particle analysis Identified molecule Protein Identification method Mass spectrometry PubMed ID 34108659     Organism Homo sapiens Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker Authors Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R. Journal name Nat Cell Biol Publication year 2021 Sample Foreskin fibroblasts Sample name BJ Isolation/purification methods Differential centrifugation Filtration Ultracentrifugation Flotation density - Molecules identified in the study Protein mRNA Methods used in the study RT-qPCR Western blotting Mass spectrometry Flow cytometry

14 Experiment ID 417 MISEV standards ✔ EM EV Biophysical techniques ✔ CD81|CD63|SDCBP|LAMP1|CD9|GAPDH|FLOT1|TFRC|FLOT2|TSG101|RAB35 EV Enriched markers ✔ CANX|ACTB EV Negative markers ✔ NTA EV Particle analysis Identified molecule Protein Identification method Mass spectrometry PubMed ID 34108659     Organism Homo sapiens Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker Authors Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R. Journal name Nat Cell Biol Publication year 2021 Sample Lymphoma cells Sample name Raji Isolation/purification methods Differential centrifugation Filtration Ultracentrifugation Flotation density - Molecules identified in the study Protein mRNA Methods used in the study RT-qPCR Western blotting Mass spectrometry Flow cytometry

15 Experiment ID 411 MISEV standards ✔ EM EV Biophysical techniques ✔ CD81|CD9|CD63|SDCBP|LAMP1|GAPDH|FLOT1|TFRC|FLOT2|TSG101|RAB35 EV Enriched markers ✔ CANX EV Negative markers ✔ NTA EV Particle analysis Identified molecule Protein Identification method Mass spectrometry PubMed ID 34108659     Organism Homo sapiens Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker Authors Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R. Journal name Nat Cell Biol Publication year 2021 Sample Mammary cancer-associated fibroblasts Sample name mCAF Isolation/purification methods Differential centrifugation Filtration Ultracentrifugation Flotation density - Molecules identified in the study Protein mRNA Methods used in the study RT-qPCR Western blotting Mass spectrometry Flow cytometry

16 Experiment ID 418 MISEV standards ✔ EM EV Biophysical techniques ✔ CD81|CD9|CD63|SDCBP|LAMP1|GAPDH|FLOT1|TFRC|FLOT2|TSG101|RAB35 EV Enriched markers ✔ CANX EV Negative markers ✔ NTA EV Particle analysis Identified molecule Protein Identification method Mass spectrometry PubMed ID 34108659     Organism Homo sapiens Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker Authors Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R. Journal name Nat Cell Biol Publication year 2021 Sample Monocytic leukemia cells Sample name THP-1 Isolation/purification methods Differential centrifugation Filtration Ultracentrifugation Flotation density - Molecules identified in the study Protein mRNA Methods used in the study RT-qPCR Western blotting Mass spectrometry Flow cytometry

17 Experiment ID 418 MISEV standards ✔ EM EV Biophysical techniques ✔ CD81|CD9|CD63|SDCBP|LAMP1|GAPDH|FLOT1|TFRC|FLOT2|TSG101|RAB35 EV Enriched markers ✔ CANX EV Negative markers ✔ NTA EV Particle analysis Identified molecule Protein Identification method Mass spectrometry PubMed ID 34108659     Organism Homo sapiens Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker Authors Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R. Journal name Nat Cell Biol Publication year 2021 Sample Monocytic leukemia cells Sample name THP-1 Isolation/purification methods Differential centrifugation Filtration Ultracentrifugation Flotation density - Molecules identified in the study Protein mRNA Methods used in the study RT-qPCR Western blotting Mass spectrometry Flow cytometry

18 Experiment ID 413 MISEV standards ✔ EM EV Biophysical techniques ✔ CD81|CD9|CD63|SDCBP|LAMP1|GAPDH|FLOT1|TFRC|FLOT2|TSG101|RAB35 EV Enriched markers ✔ CANX EV Negative markers ✔ NTA EV Particle analysis Identified molecule Protein Identification method Mass spectrometry PubMed ID 34108659     Organism Homo sapiens Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker Authors Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R. Journal name Nat Cell Biol Publication year 2021 Sample Normal mammary epithelial cells Sample name MCF10A Isolation/purification methods Differential centrifugation Filtration Ultracentrifugation Flotation density - Molecules identified in the study Protein mRNA Methods used in the study RT-qPCR Western blotting Mass spectrometry Flow cytometry

19 Experiment ID 211 MISEV standards ✔ EM EV Biophysical techniques ✔ TSG101|Alix|EpCAM|TFRC EV Enriched markers ✔ cytochrome c|GOLGA2 EV Negative markers ✘ EV Particle analysis Identified molecule protein Identification method Mass spectrometry PubMed ID 23333927     Organism Homo sapiens Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes. Authors Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K. Journal name J Proteomics Publication year 2013 Sample Ovarian cancer cells Sample name IGROV1 Isolation/purification methods Differential centrifugation Ultracentrifugation Sucrose density gradient Flotation density 1.09-1.15 g/mL Molecules identified in the study Protein Methods used in the study Mass spectrometry

20 Experiment ID 212 MISEV standards ✔ CEM EV Biophysical techniques ✔ TSG101|Alix|EpCAM|TFRC EV Enriched markers ✔ Cytochrome C|GOLGA2 EV Negative markers ✘ EV Particle analysis Identified molecule protein Identification method Mass spectrometry PubMed ID 23333927     Organism Homo sapiens Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes. Authors Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K. Journal name J Proteomics Publication year 2013 Sample Ovarian cancer cells Sample name OVCAR-3 Isolation/purification methods Differential centrifugation Ultracentrifugation Sucrose density gradient Flotation density 1.09-1.15 g/mL Molecules identified in the study Protein Methods used in the study Mass spectrometry

21 Experiment ID 406 MISEV standards ✔ EM EV Biophysical techniques ✔ CD9|CD63|SDCBP|LAMP1|GAPDH|FLOT1|TFRC|FLOT2|TSG101|RAB35|CD81 EV Enriched markers ✔ CANX EV Negative markers ✔ NTA EV Particle analysis Identified molecule Protein Identification method Mass spectrometry PubMed ID 34108659     Organism Homo sapiens Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker Authors Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R. Journal name Nat Cell Biol Publication year 2021 Sample Pancreatic cancer cells Sample name BxPC3 Isolation/purification methods Differential centrifugation Filtration Ultracentrifugation Flotation density - Molecules identified in the study Protein mRNA Methods used in the study RT-qPCR Western blotting Mass spectrometry Flow cytometry

22 Experiment ID 415 MISEV standards ✔ EM EV Biophysical techniques ✔ CD81|CD9|CD63|SDCBP|LAMP1|GAPDH|FLOT1|TFRC|FLOT2|TSG101|RAB35 EV Enriched markers ✔ CANX EV Negative markers ✔ NTA EV Particle analysis Identified molecule Protein Identification method Mass spectrometry PubMed ID 34108659     Organism Homo sapiens Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker Authors Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R. Journal name Nat Cell Biol Publication year 2021 Sample Pancreatic cancer cells Sample name PANC-1 Isolation/purification methods Differential centrifugation Filtration Ultracentrifugation Flotation density - Molecules identified in the study Protein mRNA Methods used in the study RT-qPCR Western blotting Mass spectrometry Flow cytometry

23 Experiment ID 434 MISEV standards ✘ EV Biophysical techniques ✔ SDCBP|FLOT1|CD81|CD9|CD151|FLOT2|TSG101|LAMP1|TFRC|RAB5A|RAB35|GAPDH|UCHL1|ICAM1 EV Enriched markers ✔ CANX EV Negative markers ✘ EV Particle analysis Identified molecule Protein Identification method Mass spectrometry PubMed ID 34108659     Organism Homo sapiens Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker Authors Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R. Journal name Nat Cell Biol Publication year 2021 Sample Pancreatic cancer cells Sample name PANC-1 - Exo-rich fractions 7-10 pooled Isolation/purification methods Differential centrifugation Filtration Ultracentrifugation Size exclusion chromatography Flotation density - Molecules identified in the study Protein Methods used in the study Western blotting Mass spectrometry

24 Experiment ID 435 MISEV standards ✘ EV Biophysical techniques ✔ CD81|SDCBP|FLOT1|CD9|CD151|FLOT2|TSG101|LAMP1|TFRC|RAB5A|RAB35|GAPDH|UCHL1|ICAM1 EV Enriched markers ✔ CANX EV Negative markers ✘ EV Particle analysis Identified molecule Protein Identification method Mass spectrometry PubMed ID 34108659     Organism Homo sapiens Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker Authors Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R. Journal name Nat Cell Biol Publication year 2021 Sample Pancreatic cancer cells Sample name PANC-1 - Exo-rich fractions 1-6 pooled Isolation/purification methods Differential centrifugation Filtration Ultracentrifugation OptiPrep density gradient centrifugation Flotation density - Molecules identified in the study Protein Methods used in the study Mass spectrometry

25 Experiment ID 408 MISEV standards ✔ EM EV Biophysical techniques ✔ CD9|CD63|CD81|SDCBP|LAMP1|GAPDH|FLOT1|TFRC|FLOT2|TSG101|RAB35 EV Enriched markers ✔ CANX|ACTB EV Negative markers ✔ NTA EV Particle analysis Identified molecule Protein Identification method Mass spectrometry PubMed ID 34108659     Organism Homo sapiens Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker Authors Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R. Journal name Nat Cell Biol Publication year 2021 Sample Pancreatic duct epithalial cells Sample name HPDE Isolation/purification methods Differential centrifugation Filtration Ultracentrifugation Flotation density - Molecules identified in the study Protein mRNA Methods used in the study RT-qPCR Western blotting Mass spectrometry Flow cytometry

26 Experiment ID 409 MISEV standards ✔ EM EV Biophysical techniques ✔ CD81|CD63|SDCBP|LAMP1|CD9|GAPDH|FLOT1|TFRC|FLOT2|TSG101|RAB35 EV Enriched markers ✔ CANX EV Negative markers ✔ NTA EV Particle analysis Identified molecule Protein Identification method Mass spectrometry PubMed ID 34108659     Organism Homo sapiens Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker Authors Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R. Journal name Nat Cell Biol Publication year 2021 Sample Pancreatic duct epithalial cells Sample name HPNE Isolation/purification methods Differential centrifugation Filtration Ultracentrifugation Flotation density - Molecules identified in the study Protein mRNA Methods used in the study RT-qPCR Western blotting Mass spectrometry Flow cytometry

27 Experiment ID 416 MISEV standards ✔ EM EV Biophysical techniques ✔ CD81|CD9|CD63|SDCBP|LAMP1|GAPDH|FLOT1|TFRC|FLOT2|TSG101|RAB35 EV Enriched markers ✔ CANX EV Negative markers ✔ NTA EV Particle analysis Identified molecule Protein Identification method Mass spectrometry PubMed ID 34108659     Organism Homo sapiens Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker Authors Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R. Journal name Nat Cell Biol Publication year 2021 Sample Pluripotent stem cells Sample name PSC Isolation/purification methods Differential centrifugation Filtration Ultracentrifugation Flotation density - Molecules identified in the study Protein mRNA Methods used in the study RT-qPCR Western blotting Mass spectrometry Flow cytometry

28 Experiment ID 410 MISEV standards ✔ EM EV Biophysical techniques ✔ CD81|CD63|SDCBP|LAMP1|CD9|GAPDH|FLOT1|TFRC|FLOT2|TSG101|RAB35 EV Enriched markers ✔ CANX EV Negative markers ✔ NTA EV Particle analysis Identified molecule Protein Identification method Mass spectrometry PubMed ID 34108659     Organism Homo sapiens Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker Authors Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R. Journal name Nat Cell Biol Publication year 2021 Sample T lymphocytes Sample name Jurkat Isolation/purification methods Differential centrifugation Filtration Ultracentrifugation Flotation density - Molecules identified in the study Protein mRNA Methods used in the study RT-qPCR Western blotting Mass spectrometry Flow cytometry

Protein-protein interactions for DCTPP1

Protein Interactor ExoCarta ID Identification method PubMed Species 1 FLJ10204   Two-hybrid Homo sapiens 2 DCTPP1 79077 Two-hybrid Homo sapiens Two-hybrid Homo sapiens Two-hybrid Homo sapiens Two-hybrid Homo sapiens 3 NIF3L1 60491 Two-hybrid Homo sapiens View the network

Pathways in which DCTPP1 is involved

No pathways found