Gene description for TPRKB
Gene name TP53RK binding protein
Gene symbol TPRKB
Other names/aliases CGI-121
Species Homo sapiens
 Database cross references - TPRKB
ExoCarta ExoCarta_51002
Vesiclepedia VP_51002
Entrez Gene 51002
HGNC 24259
MIM 608680
UniProt Q9Y3C4  
 TPRKB identified in sEVs derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
 Gene ontology annotations for TPRKB
Molecular Function
    protein binding GO:0005515 IPI
    protein kinase binding GO:0019901 IPI
Biological Process
    tRNA threonylcarbamoyladenosine modification GO:0002949 IBA
    tRNA threonylcarbamoyladenosine modification GO:0002949 IDA
Subcellular Localization
    EKC/KEOPS complex GO:0000408 IBA
    EKC/KEOPS complex GO:0000408 IDA
    nucleus GO:0005634 IBA
    nucleus GO:0005634 IDA
    cytoplasm GO:0005737 IDA
    cytosol GO:0005829 IBA
    cytosol GO:0005829 IDA
 Experiment description of studies that identified TPRKB in sEVs
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
 Protein-protein interactions for TPRKB
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 P3H1 64175
Co-fractionation Homo sapiens
2 COMMD10 51397
Affinity Capture-MS Homo sapiens
3 BAG3 9531
Affinity Capture-MS Homo sapiens
Affinity Capture-Luminescence Homo sapiens
4 ACTR2 10097
Co-fractionation Homo sapiens
5 POP7  
Affinity Capture-MS Homo sapiens
6 UBE2S  
Co-fractionation Homo sapiens
7 CPSF6 11052
Affinity Capture-MS Homo sapiens
8 KIF1BP 26128
Co-fractionation Homo sapiens
9 TMEM25  
Affinity Capture-MS Homo sapiens
10 NIF3L1 60491
Co-fractionation Homo sapiens
11 Osgep  
Affinity Capture-MS Mus musculus
12 ARPC1B 10095
Co-fractionation Homo sapiens
13 TPD52L2 7165
Co-fractionation Homo sapiens
14 RIT1 6016
Negative Genetic Homo sapiens
15 SH3GL1 6455
Co-fractionation Homo sapiens
16 CALU 813
Co-fractionation Homo sapiens
17 RPA2 6118
Co-fractionation Homo sapiens
18 LAP3 51056
Co-fractionation Homo sapiens
19 RANGAP1 5905
Co-fractionation Homo sapiens
20 C14orf142  
Co-fractionation Homo sapiens
21 IPO11 51194
Co-fractionation Homo sapiens
22 DBNL 28988
Co-fractionation Homo sapiens
23 ARPC2 10109
Co-fractionation Homo sapiens
24 AGFG1 3267
Co-fractionation Homo sapiens
25 PDDC1 347862
Affinity Capture-MS Homo sapiens
26 OSGEP 55644
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
27 NUP43 348995
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
28 C8orf76  
Affinity Capture-MS Homo sapiens
29 POLR3E 55718
Affinity Capture-MS Homo sapiens
30 GORASP1 64689
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
31 ANO4 121601
Affinity Capture-MS Homo sapiens
32 G6PD 2539
Co-fractionation Homo sapiens
33 PRAME  
Affinity Capture-Western Homo sapiens
34 MUT 4594
Co-fractionation Homo sapiens
35 SPOCK2  
Affinity Capture-MS Homo sapiens
36 MBIP  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
37 LAGE3  
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
38 ARPC4 10093
Co-fractionation Homo sapiens
39 SEC31A 22872
Co-fractionation Homo sapiens
40 SH3GLB1 51100
Co-fractionation Homo sapiens
41 HSF1  
Co-fractionation Homo sapiens
42 EGFR 1956
Negative Genetic Homo sapiens
43 MSH5  
Affinity Capture-MS Homo sapiens
44 ACTR3 10096
Co-fractionation Homo sapiens
45 TRIM27  
Two-hybrid Homo sapiens
46 GDI1 2664
Co-fractionation Homo sapiens
47 TP53RK 112858
Two-hybrid Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Co-fractionation Homo sapiens
48 PRKACB 5567
Co-fractionation Homo sapiens
49 THG1L 54974
Co-fractionation Homo sapiens
50 KRAS 3845
Negative Genetic Homo sapiens
51 ARPC5 10092
Co-fractionation Homo sapiens
52 LPP 4026
Co-fractionation Homo sapiens
53 TRMT1 55621
Co-fractionation Homo sapiens
54 METTL1 4234
Co-fractionation Homo sapiens
55 HSPBP1 23640
Co-fractionation Homo sapiens
56 ARPC1A 10552
Co-fractionation Homo sapiens
View the network image/svg+xml
 Pathways in which TPRKB is involved
PathwayEvidenceSource
Metabolism of RNA IEA Reactome
tRNA modification in the nucleus and cytosol IEA Reactome
tRNA processing IEA Reactome





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