Gene description for GNMT
Gene name glycine N-methyltransferase
Gene symbol GNMT
Other names/aliases -
Species Homo sapiens
 Database cross references - GNMT
ExoCarta ExoCarta_27232
Vesiclepedia VP_27232
Entrez Gene 27232
HGNC 4415
MIM 606628
UniProt Q14749  
 GNMT identified in exosomes derived from the following tissue/cell type
Chondrocytes 35931686    
Chondrocytes 35931686    
 Gene ontology annotations for GNMT
Molecular Function
    protein binding GO:0005515 IPI
    glycine binding GO:0016594 IDA
    glycine N-methyltransferase activity GO:0017174 IDA
    folic acid binding GO:0005542 IEA
Biological Process
    methylation GO:0032259 IEA
    glycogen metabolic process GO:0005977 IEA
    S-adenosylmethionine metabolic process GO:0046500 IDA
    regulation of gluconeogenesis GO:0006111 IEA
    methionine metabolic process GO:0006555 IEA
    cellular protein modification process GO:0006464 NAS
    one-carbon metabolic process GO:0006730 IEA
    protein homotetramerization GO:0051289 IPI
Subcellular Localization
    cytoplasm GO:0005737 IDA
 Experiment description of studies that identified GNMT in exosomes
1
Experiment ID 494
MISEV standards
✔
EM
EV Biophysical techniques
✔
CD9|CD81|CD151|CD63|CD82|FLOT1|FLOT2|GAPDH|LAMP1|LAMP2|SDCBP|TFRC|TSG101
EV Enriched markers
✘ EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35931686    
Organism Homo sapiens
Experiment description Extracellular vesicles enriched in connexin 43 promote a senescent phenotype in bone and synovial cells contributing to osteoarthritis progression
Authors Varela-Eirín M, Carpintero-Fernández P, Guitián-Caamaño A, Varela-Vázquez A, García-Yuste A, Sánchez-Temprano A, Bravo-López SB, Yañez-Cabanas J, Fonseca E, Largo R, Mobasheri A, Caeiro JR, Mayán MD.
Journal name Cell Death Dis
Publication year 2022
Sample Chondrocytes
Sample name Osteoarthritic cartilage
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 496
MISEV standards
✘ EV Biophysical techniques
✔
CD9|CD151|CD63|CD81|CD82|FLOT1|FLOT2|GAPDH|LAMP1|LAMP2|SDCBP|TFRC|TSG101
EV Enriched markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35931686    
Organism Homo sapiens
Experiment description Extracellular vesicles enriched in connexin 43 promote a senescent phenotype in bone and synovial cells contributing to osteoarthritis progression
Authors Varela-Eirín M, Carpintero-Fernández P, Guitián-Caamaño A, Varela-Vázquez A, García-Yuste A, Sánchez-Temprano A, Bravo-López SB, Yañez-Cabanas J, Fonseca E, Largo R, Mobasheri A, Caeiro JR, Mayán MD.
Journal name Cell Death Dis
Publication year 2022
Sample Chondrocytes
Sample name Healthy cartilage
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
 Protein-protein interactions for GNMT
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 FLJ22494  
Two-hybrid Homo sapiens
2 GNMT 27232
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
3 ARR3 407
Two-hybrid Homo sapiens
4 PLEKHF2 79666
Two-hybrid Homo sapiens
5 NIF3L1 60491
Two-hybrid Homo sapiens
6 ARRB1 408
Two-hybrid Homo sapiens
View the network image/svg+xml
 Pathways in which GNMT is involved
No pathways found





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