Gene description for GNMT
Gene name glycine N-methyltransferase
Gene symbol GNMT
Other names/aliases -
Species Homo sapiens
 Database cross references - GNMT
ExoCarta ExoCarta_27232
Vesiclepedia VP_27232
Entrez Gene 27232
HGNC 4415
MIM 606628
UniProt Q14749  
 GNMT identified in exosomes derived from the following tissue/cell type
Chondrocytes 35931686    
Chondrocytes 35931686    
 Gene ontology annotations for GNMT
Molecular Function
    protein binding GO:0005515 IPI
    folic acid binding GO:0005542 IEA
    glycine binding GO:0016594 IBA
    glycine binding GO:0016594 IDA
    glycine N-methyltransferase activity GO:0017174 IBA
    glycine N-methyltransferase activity GO:0017174 IDA
    identical protein binding GO:0042802 IBA
    identical protein binding GO:0042802 IPI
    S-adenosyl-L-methionine binding GO:1904047 IBA
Biological Process
    glycogen metabolic process GO:0005977 IEA
    regulation of gluconeogenesis GO:0006111 IBA
    methionine metabolic process GO:0006555 IEA
    one-carbon metabolic process GO:0006730 IBA
    methylation GO:0032259 IEA
    protein modification process GO:0036211 NAS
    S-adenosylhomocysteine metabolic process GO:0046498 IBA
    S-adenosylmethionine metabolic process GO:0046500 IBA
    S-adenosylmethionine metabolic process GO:0046500 IDA
    protein homotetramerization GO:0051289 IBA
    protein homotetramerization GO:0051289 IPI
    sarcosine metabolic process GO:1901052 IBA
Subcellular Localization
    cytosol GO:0005829 IBA
    cytosol GO:0005829 IDA
    cytosol GO:0005829 TAS
 Experiment description of studies that identified GNMT in exosomes
1
Experiment ID 494
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD81|CD151|CD63|CD82|FLOT1|FLOT2|GAPDH|LAMP1|LAMP2|SDCBP|TFRC|TSG101
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35931686    
Organism Homo sapiens
Experiment description Extracellular vesicles enriched in connexin 43 promote a senescent phenotype in bone and synovial cells contributing to osteoarthritis progression
Authors "Varela-Eirín M, Carpintero-Fernández P, Guitián-Caamaño A, Varela-Vázquez A, García-Yuste A, Sánchez-Temprano A, Bravo-López SB, Yañez-Cabanas J, Fonseca E, Largo R, Mobasheri A, Caeiro JR, Mayán MD."
Journal name Cell Death Dis
Publication year 2022
Sample Chondrocytes
Sample name Osteoarthritic cartilage
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 496
MISEV standards
✘ Biophysical techniques
✔
CD9|CD151|CD63|CD81|CD82|FLOT1|FLOT2|GAPDH|LAMP1|LAMP2|SDCBP|TFRC|TSG101
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35931686    
Organism Homo sapiens
Experiment description Extracellular vesicles enriched in connexin 43 promote a senescent phenotype in bone and synovial cells contributing to osteoarthritis progression
Authors "Varela-Eirín M, Carpintero-Fernández P, Guitián-Caamaño A, Varela-Vázquez A, García-Yuste A, Sánchez-Temprano A, Bravo-López SB, Yañez-Cabanas J, Fonseca E, Largo R, Mobasheri A, Caeiro JR, Mayán MD."
Journal name Cell Death Dis
Publication year 2022
Sample Chondrocytes
Sample name Healthy cartilage
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
 Protein-protein interactions for GNMT
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 RPSA 3921
Two-hybrid Homo sapiens
2 NOTCH2NL 388677
Two-hybrid Homo sapiens
3 GNMT 27232
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
4 SDCBP 6386
Two-hybrid Homo sapiens
5 TERF2  
Two-hybrid Homo sapiens
6 NIF3L1 60491
Two-hybrid Homo sapiens
7 FRG1 2483
Two-hybrid Homo sapiens
8 POT1  
Two-hybrid Homo sapiens
9 PREX2  
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
10 SRD5A2  
Two-hybrid Homo sapiens
11 ADAMTSL4  
Two-hybrid Homo sapiens
12 ZNF337  
Two-hybrid Homo sapiens
13 ARR3 407
Two-hybrid Homo sapiens
14 PLEKHF2 79666
Two-hybrid Homo sapiens
15 GABRQ  
Two-hybrid Homo sapiens
16 TERF2IP 54386
Two-hybrid Homo sapiens
17 KRTAP10-3  
Two-hybrid Homo sapiens
18 NUDT18  
Two-hybrid Homo sapiens
19 CCDC153  
Two-hybrid Homo sapiens
20 SNRNP200 23020
Two-hybrid Homo sapiens
21 TERF1 7013
Two-hybrid Homo sapiens
22 HDAC7  
Two-hybrid Homo sapiens
23 LACRT 90070
Affinity Capture-MS Homo sapiens
24 WDYHV1  
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
25 ARRB1 408
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
26 TRIM35 23087
Two-hybrid Homo sapiens
27 ATP1B2  
Affinity Capture-MS Homo sapiens
28 HPRT1 3251
Two-hybrid Homo sapiens
29 NSF 4905
Affinity Capture-MS Homo sapiens
30 COX7B 1349
Two-hybrid Homo sapiens
31 KRTAP4-2  
Two-hybrid Homo sapiens
32 TINF2  
Two-hybrid Homo sapiens
33 KRT222 125113
Affinity Capture-MS Homo sapiens
34 HUWE1 10075
Affinity Capture-Western Homo sapiens
35 GAGE2A  
Two-hybrid Homo sapiens
View the network image/svg+xml



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