Gene description for FBXW4
Gene name F-box and WD repeat domain containing 4
Gene symbol FBXW4
Other names/aliases DAC
FBW4
FBWD4
SHFM3
SHSF3
Species Homo sapiens
 Database cross references - FBXW4
ExoCarta ExoCarta_6468
Vesiclepedia VP_6468
Entrez Gene 6468
HGNC 10847
MIM 608071
UniProt P57775  
 FBXW4 identified in sEVs derived from the following tissue/cell type
Hepatocytes 26054723    
 Gene ontology annotations for FBXW4
Molecular Function
    protein binding GO:0005515 IPI
Biological Process
    ubiquitin-dependent protein catabolic process GO:0006511 NAS
    Wnt signaling pathway GO:0016055 IEA
    embryonic limb morphogenesis GO:0030326 NAS
    SCF-dependent proteasomal ubiquitin-dependent protein catabolic process GO:0031146 IBA
Subcellular Localization
    ubiquitin ligase complex GO:0000151 NAS
    cytosol GO:0005829 TAS
    SCF ubiquitin ligase complex GO:0019005 IBA
 Experiment description of studies that identified FBXW4 in sEVs
1
Experiment ID 237
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|Alix|HSC70|GAPDH
Enriched markers
✔
HSP90B1
Negative markers
✔
qNano
Particle analysis
Identified molecule mRNA
Identification method RNA Sequencing
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors "He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N"
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocytes
Sample name MIHA
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RNA Sequencing
 Protein-protein interactions for FBXW4
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 YWHAH 7533
Affinity Capture-MS Homo sapiens
2 ATP5H 10476
Affinity Capture-MS Homo sapiens
3 PFDN1 5201
Affinity Capture-MS Homo sapiens
4 CUL1 8454
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
5 SLC25A11 8402
Affinity Capture-MS Homo sapiens
6 GPS1 2873
Affinity Capture-MS Homo sapiens
7 CCT8 10694
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
8 COPS2 9318
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
9 NUP205 23165
Affinity Capture-MS Homo sapiens
10 COPS7A 50813
Affinity Capture-MS Homo sapiens
11 SKIV2L 6499
Affinity Capture-MS Homo sapiens
12 CCT2 10576
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
13 PDCL 5082
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
14 COPS7B 64708
Affinity Capture-MS Homo sapiens
15 CCT6A 908
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
16 CDC37 11140
Two-hybrid Homo sapiens
17 TCP1 6950
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
18 COPS8 10920
Affinity Capture-MS Homo sapiens
19 SKP1 6500
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
20 PSMA2 5683
Affinity Capture-MS Homo sapiens
21 RNF32  
Two-hybrid Homo sapiens
22 CCT4 10575
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
23 CCT6B 10693
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
24 HNRNPR 10236
Affinity Capture-MS Homo sapiens
25 MCM4 4173
Affinity Capture-MS Homo sapiens
26 ANKRD39  
Affinity Capture-MS Homo sapiens
27 DDX58 23586
Affinity Capture-RNA Homo sapiens
28 A2M 2
Two-hybrid Homo sapiens
29 CCT7 10574
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
30 TXNDC9 10190
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
31 CCT5 22948
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
32 SEMG1 6406
Affinity Capture-MS Homo sapiens
33 PFDN5 5204
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
34 RUVBL1 8607
Affinity Capture-MS Homo sapiens
35 PFDN2 5202
Affinity Capture-MS Homo sapiens
36 PPP2R1A 5518
Affinity Capture-MS Homo sapiens
37 SUPT6H 6830
Affinity Capture-MS Homo sapiens
38 PPP2R1B 5519
Affinity Capture-MS Homo sapiens
39 DDX5 1655
Affinity Capture-MS Homo sapiens
40 COPS6 10980
Affinity Capture-MS Homo sapiens
41 DCTN1 1639
Affinity Capture-MS Homo sapiens
42 COPS5 10987
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
43 CCT3 7203
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
44 COPS3 8533
Affinity Capture-MS Homo sapiens
45 DHX9 1660
Affinity Capture-MS Homo sapiens
46 PDCL3 79031
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
47 COPS4 51138
Affinity Capture-MS Homo sapiens
48 SLX4IP  
Affinity Capture-MS Homo sapiens
49 MAST1  
Two-hybrid Homo sapiens
50 REST 5978
Affinity Capture-Western Homo sapiens
51 RCAN1  
Affinity Capture-Western Homo sapiens
52 PDRG1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
53 NLK  
Affinity Capture-Western Homo sapiens
54 CCDC8  
Affinity Capture-MS Homo sapiens
55 ECSIT 51295
Two-hybrid Homo sapiens
View the network image/svg+xml



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