Gene description for REST
Gene name RE1-silencing transcription factor
Gene symbol REST
Other names/aliases NRSF
XBR
Species Homo sapiens
 Database cross references - REST
ExoCarta ExoCarta_5978
Entrez Gene 5978
HGNC 9966
MIM 600571
UniProt Q13127  
 REST identified in exosomes derived from the following tissue/cell type
Endothelial cells 26027894    
 Gene ontology annotations for REST
Molecular Function
    core promoter binding GO:0001047 IDA
    RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in negative regulation of transcription GO:0001078 IDA
    core promoter sequence-specific DNA binding GO:0001046 IDA
    sequence-specific DNA binding transcription factor activity GO:0003700 IDA
    chromatin binding GO:0003682 ISS
    transcription factor binding GO:0008134 IPI
    outward rectifier potassium channel activity GO:0015271 IMP
    protein binding GO:0005515 IPI
    transcription regulatory region DNA binding GO:0044212 IDA
    metal ion binding GO:0046872 IEA
Biological Process
    negative regulation of insulin secretion GO:0046676 IMP
    transcription from RNA polymerase II promoter GO:0006366 IEA
    negative regulation of transcription, DNA-templated GO:0045892 NAS
    negative regulation of neurogenesis GO:0050768 ISS
    positive regulation of cysteine-type endopeptidase activity involved in apoptotic process GO:0043280 IMP
    regulation of transcription, DNA-templated GO:0006355 NAS
    negative regulation of amniotic stem cell differentiation GO:2000798 IMP
    negative regulation of mesenchymal stem cell differentiation GO:2000740 IMP
    cellular response to drug GO:0035690 IMP
    hematopoietic progenitor cell differentiation GO:0002244 IEA
    negative regulation of gene expression GO:0010629 IMP
    positive regulation of transcription, DNA-templated GO:0045893 IDA
    cellular response to glucocorticoid stimulus GO:0071385 IDA
    chromatin organization GO:0006325 TAS
    negative regulation of aldosterone biosynthetic process GO:0032348 IMP
    positive regulation of apoptotic process GO:0043065 IMP
    negative regulation of dense core granule biogenesis GO:2000706 ISS
    cardiac muscle cell myoblast differentiation GO:0060379 ISS
    potassium ion transmembrane transport GO:0071805 IMP
    negative regulation by host of viral transcription GO:0043922 IDA
    negative regulation of cortisol biosynthetic process GO:2000065 IMP
    histone H4 deacetylation GO:0070933 IDA
    negative regulation of cell proliferation GO:0008285 IMP
    negative regulation of neuron differentiation GO:0045665 IMP
    negative regulation of transcription from RNA polymerase II promoter GO:0000122 TAS
    cellular response to electrical stimulus GO:0071257 IMP
    negative regulation of calcium ion-dependent exocytosis GO:0045955 ISS
Subcellular Localization
    cytosol GO:0005829 IEA
    nucleoplasm GO:0005654 TAS
    transcriptional repressor complex GO:0017053 IDA
    nucleus GO:0005634 NAS
 Experiment description of studies that identified REST in exosomes
1
Experiment ID 226
ISEV standards
EM
EV Biophysical techniques
GAPDH
EV Cytosolic markers
CD9|FLOT1
EV Membrane markers
LMNA|H2AFX|ATP5A1|TOMM20
EV Negative markers
EV Particle analysis
Identified molecule mRNA
Identification method Small RNA sequencing (Illumina HiSeq 2000 (Solexa)
PubMed ID 26027894    
Organism Homo sapiens
Experiment description Quantitative and qualitative analysis of small RNAs in human endothelial cells and exosomes provides insights into localized RNA processing, degradation and sorting
Authors Bas W. M. van Balkom, Almut S. Eisele, D. Michiel Pegtel, Sander Bervoets, Marianne C. Verhaar
Journal name Journal of Extracellular Vesicles
Publication year 2015
Sample Endothelial cells
Sample name HMEC-1
Isolation/purification methods Differential ultracentrifugation
Sucrose density gradient
Flotation density 1.10 g/mL
Molecules identified in the study miRNA
Protein
snoRNA
lncRNA
yRNA
snRNA
mRNA
ncRNA
mtRNA
vtRNA
scaRNA
lincRNA
Methods used in the study Small RNA sequencing (Illumina HiSeq 2000 (Solexa)
Western blotting
 Protein-protein interactions for REST
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 RCOR1  
Invivo Homo sapiens
Invitro Homo sapiens
Two-hybrid Homo sapiens
2 SIN3B  
Invivo Homo sapiens
Invitro Homo sapiens
Two-hybrid Homo sapiens
View the network image/svg+xml
 Pathways in which REST is involved
PathwayEvidenceSource
HDACs deacetylate histones TAS Reactome





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