Gene description for GTF2B
Gene name general transcription factor IIB
Gene symbol GTF2B
Other names/aliases TF2B
TFIIB
Species Homo sapiens
 Database cross references - GTF2B
ExoCarta ExoCarta_2959
Entrez Gene 2959
HGNC 4648
MIM 189963
UniProt Q00403  
 GTF2B identified in exosomes derived from the following tissue/cell type
Thymus 23844026    
 Gene ontology annotations for GTF2B
Molecular Function
    protein binding GO:0005515 IPI
    TBP-class protein binding GO:0017025 IEA
    thyroid hormone receptor binding GO:0046966 IPI
    zinc ion binding GO:0008270 IEA
    transcription factor binding GO:0008134 IPI
    core promoter binding GO:0001047 IEA
Biological Process
    transcription from RNA polymerase II promoter GO:0006366 TAS
    gene expression GO:0010467 TAS
    transcription elongation from RNA polymerase II promoter GO:0006368 TAS
    viral process GO:0016032 TAS
    transcription initiation from RNA polymerase II promoter GO:0006367 TAS
    regulation of transcription, DNA-templated GO:0006355 IEA
Subcellular Localization
    nucleus GO:0005634 IDA
    nucleoplasm GO:0005654 TAS
    nucleolus GO:0005730 IDA
 Experiment description of studies that identified GTF2B in exosomes
1
Experiment ID 217
ISEV standards
EM
EV Biophysical techniques
TSG101
EV Cytosolic markers
CD81|CD9|CD63
EV Membrane markers
EV Negative markers
NTA
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23844026    
Organism Homo sapiens
Experiment description Characterization of human thymic exosomes.
Authors Skogberg G, Gudmundsdottir J, van der Post S, Sandstrom K, Bruhn S, Benson M, Mincheva-Nilsson L, Baranov V, Telemo E, Ekwall O.
Journal name PLoS One
Publication year 2013
Sample Thymus
Sample name Normal-Thymus
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for GTF2B
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 FOXF2  
Invitro Homo sapiens
2 GTF2F1 2962
Co-purification Homo sapiens
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
3 HSF1  
Reconstituted Complex Homo sapiens
4 GTF2E1  
Invitro Homo sapiens
Reconstituted Complex Homo sapiens
Co-purification Homo sapiens
5 NCOA1  
Reconstituted Complex Homo sapiens
6 REL 5966
Reconstituted Complex Homo sapiens
7 NOLC1 9221
Invitro Homo sapiens
8 GTF2F2  
Reconstituted Complex Homo sapiens
9 RELA  
Invitro Homo sapiens
10 CREBBP  
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
11 BCL3  
Reconstituted Complex Homo sapiens
12 CTBP1 1487
Reconstituted Complex Homo sapiens
13 IKZF1  
Invitro Homo sapiens
Reconstituted Complex Homo sapiens
14 POLR2A 5430
Co-purification Homo sapiens
Reconstituted Complex Homo sapiens
15 TAF11  
Co-purification Homo sapiens
Reconstituted Complex Homo sapiens
16 TBP  
Co-crystal Structure Homo sapiens
Co-purification Homo sapiens
Reconstituted Complex Homo sapiens
17 JUN 3725
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
18 MED21  
Affinity Capture-Western Homo sapiens
19 GTF2H4  
Co-purification Homo sapiens
Reconstituted Complex Homo sapiens
20 MYC  
Reconstituted Complex Homo sapiens
21 GTF2E2  
Invitro Homo sapiens
Affinity Capture-Western Homo sapiens
22 MCM2 4171
Invitro Homo sapiens
23 NR2F1  
Invitro Homo sapiens
24 NR5A1  
Invitro Homo sapiens
25 PSMC2 5701
Invivo Homo sapiens
Invitro Homo sapiens
26 TBPL1  
Reconstituted Complex Homo sapiens
27 NCOA4 8031
Reconstituted Complex Homo sapiens
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