Gene description for DRAP1
Gene name DR1-associated protein 1 (negative cofactor 2 alpha)
Gene symbol DRAP1
Other names/aliases NC2-alpha
Species Homo sapiens
 Database cross references - DRAP1
ExoCarta ExoCarta_10589
Vesiclepedia VP_10589
Entrez Gene 10589
HGNC 3019
MIM 602289
UniProt Q14919  
 DRAP1 identified in exosomes derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Chondrocytes 35931686    
Chondrocytes 35931686    
 Gene ontology annotations for DRAP1
Molecular Function
    core promoter sequence-specific DNA binding GO:0001046 IBA
    RNA polymerase II general transcription initiation factor binding GO:0001091 IDA
    RNA polymerase II general transcription initiation factor binding GO:0001091 IPI
    DNA binding GO:0003677 IDA
    protein binding GO:0005515 IPI
    RNA polymerase II general transcription initiation factor activity GO:0016251 IBA
    RNA polymerase II general transcription initiation factor activity GO:0016251 IDA
    TBP-class protein binding GO:0017025 IPI
    identical protein binding GO:0042802 IPI
    protein heterodimerization activity GO:0046982 IEA
Biological Process
    negative regulation of transcription by RNA polymerase II GO:0000122 IDA
    transcription by RNA polymerase II GO:0006366 IBA
Subcellular Localization
    nucleus GO:0005634 IBA
    negative cofactor 2 complex GO:0017054 IBA
    RNA polymerase II transcription regulator complex GO:0090575 IDA
 Experiment description of studies that identified DRAP1 in exosomes
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 497
MISEV standards
✘ Biophysical techniques
✔
CD9|CD151|CD63|CD81|CD82|FLOT1|FLOT2|GAPDH|LAMP1|LAMP2|RAB5A|SDCBP|TFRC|TSG101|UCHL1
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35931686    
Organism Homo sapiens
Experiment description Extracellular vesicles enriched in connexin 43 promote a senescent phenotype in bone and synovial cells contributing to osteoarthritis progression
Authors "Varela-Eirín M, Carpintero-Fernández P, Guitián-Caamaño A, Varela-Vázquez A, García-Yuste A, Sánchez-Temprano A, Bravo-López SB, Yañez-Cabanas J, Fonseca E, Largo R, Mobasheri A, Caeiro JR, Mayán MD."
Journal name Cell Death Dis
Publication year 2022
Sample Chondrocytes
Sample name T/C-28a2
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
6
Experiment ID 498
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD151|CD63|CD81|CD82|FLOT1|FLOT2|GAPDH|LAMP1|LAMP2|RAB5A|SDCBP|TFRC|TSG101|UCHL1
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35931686    
Organism Homo sapiens
Experiment description Extracellular vesicles enriched in connexin 43 promote a senescent phenotype in bone and synovial cells contributing to osteoarthritis progression
Authors "Varela-Eirín M, Carpintero-Fernández P, Guitián-Caamaño A, Varela-Vázquez A, García-Yuste A, Sánchez-Temprano A, Bravo-López SB, Yañez-Cabanas J, Fonseca E, Largo R, Mobasheri A, Caeiro JR, Mayán MD."
Journal name Cell Death Dis
Publication year 2022
Sample Chondrocytes
Sample name T/C-28a2
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
 Protein-protein interactions for DRAP1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 PTPRF 5792
Cross-Linking-MS (XL-MS) Homo sapiens
2 MCRS1  
Two-hybrid Homo sapiens
3 FOXH1  
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
4 PRKCSH 5589
Co-fractionation Homo sapiens
5 SDCBP 6386
Two-hybrid Homo sapiens
6 MED17  
Affinity Capture-MS Homo sapiens
7 TK1 7083
Two-hybrid Homo sapiens
8 RBM25 58517
Co-fractionation Homo sapiens
9 TPD52L2 7165
Co-fractionation Homo sapiens
10 SRPK2 6733
Biochemical Activity Homo sapiens
11 MRPS11  
Affinity Capture-MS Homo sapiens
12 ZEB1  
Two-hybrid Homo sapiens
13 HSPBP1 23640
Co-fractionation Homo sapiens
14 BTF3 689
Affinity Capture-MS Homo sapiens
15 RAD23B 5887
Co-fractionation Homo sapiens
16 DR1  
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Co-fractionation Homo sapiens
Affinity Capture-Luminescence Homo sapiens
Affinity Capture-MS Homo sapiens
Cross-Linking-MS (XL-MS) Homo sapiens
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Co-fractionation Homo sapiens
Two-hybrid Homo sapiens
17 FEZ1  
Two-hybrid Homo sapiens
Reconstituted Complex Homo sapiens
18 PLEKHF2 79666
Two-hybrid Homo sapiens
19 RPL9 6133
Two-hybrid Homo sapiens
20 HNRNPA1 3178
Cross-Linking-MS (XL-MS) Homo sapiens
21 MAP1B 4131
Cross-Linking-MS (XL-MS) Homo sapiens
22 TTF2 8458
Two-hybrid Homo sapiens
23 MORF4L1  
Two-hybrid Homo sapiens
24 POLR2A 5430
Co-fractionation Homo sapiens
25 GTF2B 2959
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
26 APP 351
Reconstituted Complex Homo sapiens
27 FOXD3  
Affinity Capture-MS Homo sapiens
28 NCK2 8440
Two-hybrid Homo sapiens
29 TBP  
Affinity Capture-MS Homo sapiens
Co-crystal Structure Homo sapiens
Affinity Capture-Western Homo sapiens
30 LMO2  
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
31 TAF9B  
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
32 SNRNP70 6625
Co-fractionation Homo sapiens
33 NFYB  
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
34 TAF9 6880
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
35 DRAP1 10589
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
36 GTF2F1 2962
Co-fractionation Homo sapiens
37 FOXK2  
Affinity Capture-MS Homo sapiens
38 GTF2E1 2960
Co-fractionation Homo sapiens
39 GAPDH 2597
Cross-Linking-MS (XL-MS) Homo sapiens
40 BRCC3 79184
Co-fractionation Homo sapiens
41 SUMO3 6612
Co-fractionation Homo sapiens
42 ABCE1 6059
Cross-Linking-MS (XL-MS) Homo sapiens
43 TNFRSF14  
Two-hybrid Homo sapiens
44 POLE3  
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
45 FEZ2 9637
Two-hybrid Homo sapiens
46 CDKN2C  
Two-hybrid Homo sapiens
47 DNAJB11 51726
Two-hybrid Homo sapiens
48 PIK3R3 8503
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
49 RPL19 6143
Cross-Linking-MS (XL-MS) Homo sapiens
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